Comprehensive In-Service Training Program to Enhance Informatics Competency for Nurses in Taiwan

Chu Li Tseng,Zu Chun Lin,Yu Chin Lee,Huea Hwa Jiang,Li Li Li,Yu Lin Han 한국간호과학회 2011 한국간호과학회 학술대회 Vol.2011 No.10

Proteomic Analysis of the Aging-related Proteins in Human Normal Colon Epithelial Tissue

Li, Ming,Xiao, Zhi-Qiang,Chen, Zhu-Chu,Li, Jian-Ling,Li, Cui,Zhang, Peng-Fei,Li, Mao-Yu Korean Society for Biochemistry and Molecular Biol 2007 Journal of biochemistry and molecular biology Vol.40 No.1

In order to screen the aging related proteins in human normal colon epithelia, the comparative proteomics analysis was applied to get the two-dimensional electrophoresis (2-DE) profiles with high resolution and reproducibility from normal colon epithelial tissues of young and aged people. Differential proteins between the colon epithelia of two age groups were found with PDQuest software. The thirty five differential protein-spots were identified by peptide mass fingerprint (PMF) based on matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOFMS) and database searching. Among them there are sixteen proteins which are significantly up-regulated in the colonic mucosal epithelia of young people group, which include ATP synthase beta chain, electron transfer flavoprotein alpha-subunit, catalase, glutathione peroxidase 1, annexin A2 and heat shock cognate 71 kDa protein, etc.; There are nineteen proteins which are significantly up-regulated in the colonic mucosal epithelia of aged people group, which include far upstream element-binding protein 1, nucleoside diphosphate kinase B, protein disulfide-isomerase precursor and VDAC-2, etc.. The identified differential proteins appear to be involved in metabolism, energy generation, chaperone, antioxidation, signal transduction, protein folding and apoptosis. The data will help to understand the molecular mechanisms of human colon epithelial aging.

Proteomic Analysis of the Aging-related Proteins in Human Normal Colon Epithelial Tissue

( Ming Li ),( Zhi Qiang Xiao ),( Zhu Chu Chen ),( Jian Ling Li ),( Cui Li ),( Peng Fei Zhang ),( Mao Yu Li ) 생화학분자생물학회 2007 BMB Reports Vol.40 No.1

Fertility and prognosis assessment between bleomycin/etoposide/cisplatin and paclitaxel/carboplatin chemotherapy regimens in the conservative treatment of malignant ovarian germ cell tumors: a multicenter and retrospective study

Ran Chu,Penglin Liu,Jingying Chen,Xiaodong Cheng,Kezhen Li,Yanci Che,Jianliu Wang,Li Li,Xi Zhang,Shu Yao,Li Song,Ying Zhao,Changzhen Huang,Ying Xue,Xiyu Pan,Junting Li,Zhongshao Chen,Jie Jiang,Beihua 대한부인종양학회 2023 Journal of Gynecologic Oncology Vol.34 No.2

Objective: To evaluate the impact of bleomycin/etoposide/cisplatin (BEP) and paclitaxel/carboplatin (PC) chemotherapy regimens on the fertility and prognostic outcomes in malignant ovarian germ cell tumor (MOGCT) patients who underwent fertility-sparing surgery (FSS). Methods: A propensity score matching algorithm was performed between the BEP and PC groups. The χ2 test and the Kaplan-Meier method were used to compare the fertility outcome, disease-free survival (DFS) and overall survival (OS). The Cox proportional hazards regression analysis was used to identify risk factor of DFS. Results: We included 213 patients, 185 (86.9%) underwent BEP chemotherapy, and 28 (13.1%) underwent PC chemotherapy. The median age was 22 years (range, 8–44 years), and the median follow-up period was 63 months (range, 2–191 months). Fifty-one (29.3%) patients had a pregnancy plan, and 35 (85.4%) delivered successfully. In the before and after propensity score matching cohorts, there were no significant differences in spontaneous abortion, selective termination of pregnancy, during-pregnancy status, and live birth between the BEP and PC groups (p>0.05). Fourteen (6.6%) patients experienced recurrence, including 11 (5.9%) in the BEP group and 3 (10.7%) in the PC group. Four (1.9%) patients in the BEP group died. Kaplan-Meier analysis revealed no significant differences in DFS (p=0.328) and OS (p=0.446) between the BEP and PC groups, and the same survival results were observed in the after matching cohort. Conclusion: The PC regimen is as safe as the BEP regimen for MOGCT patients with fertility preservation treatment, and no differences were observed in fertility and clinical prognosis.

Reduced expression of HSP27 following HAD-B treatment is associated with Her2 downregulation in NIH:OVCAR-3 human ovarian cancer cells

LI, KUO CHU,HEO, KYUN,AMBADE, NITIN,KIM, MIN KYUNG,KIM, KYUNG-HEE,YOO, BYONG CHUL,YOO, HWA-SEUNG SPANDIDOS PUBLICATIONS 2015 MOLECULAR MEDICINE REPORTS Vol.12 No.3

<P>The Korean traditional medicine, HangAmDan (HAD), was developed in 1996 for use as an antitumor agent, and has since been modified to HAD?B (an altered form of HAD), in order to potentiate its therapeutic effects. In the present study, the effect of HAD?B on the proliferation and invasion of NIH:OVCAR?3 and SKOV?3 human ovarian cancer cell lines was investigated. In addition, the expression of major signal transduction molecules and changes in the proteome in these cells were measured. HAD?B treatment effectively induced a reduction in the levels of cell proliferation in serum?free conditioned media. However, unaltered levels of PARP and caspase?3 indicated that HAD?B does not reduce proliferation by inducing apoptotic cell death. Fluorescence?activated cell sorting analysis revealed no significant change in apoptosis following HAD-B treatment. Invasion assay results indicated a reduced rate of invasion following HAD?B treatment. HAD?B also influenced the expression of major signal transduction molecules; the phosphorylation of mTOR and AKT was reduced, while that of ERK was increased. Alterations in the proteomes of the two cell lines were investigated following HAD?B treatment. Among the 9 proteins with differential expression, heat?shock protein β?1 (HSP27) was downregulated in NIH:OVCAR?3 cells treated with HAD?B. The reduced expression of HSP27 was associated with human epidermal growth factor receptor 2 (Her2) downregulation in these cells. In conclusion, the results of the current proteome assessment suggest that HAD?B has the potential to suppress the proliferation and invasion of human ovarian cancer cells. HAD?B treatment of NIH:OVCAR?3 cells suppressed HSP27 expression and was also associated with Her2 downregulation.</P>

Research Paper : Effects of phosphorus and light intensity on the growth and competition of the two weed species Veronica persica and Chorispora tenella

( Li Chu Yin ),( Jian Xin Luo ),( Lan Fang Luo ),( Gui Xian Xie ) 한국잡초학회 2012 Weed Biology and Management Vol.12 No.1

Veronica persica Poir. (Persian speedwell) is an invasive alien weed species in the upland soils throughout China. Its dominance in weed flora could be alleviated by Chorispora tenella (Pall.) (tender chorispora) in P-poor soils and in an intense light environment. Here, three experiments in growth chambers were carried out in order to explore the effect of P and light intensity on the growth and competition of V. persica and C. tenella, the pH value in their rhizospheres, and the kinetic parameters (Km and Vmax) of the H2PO4 - uptake by the two weed species. The ANOVA results showed that the light intensity and P level in the soil obviously influenced the growth of the two weed species. However, the effect of the P level in the soil was greater for V. persica and the effect of the light intensity was greater for C. tenella. The restriction of the growth of V. persica by a low P level in the soil was much more than that of C. tenella, but it was reversed in the two species in relation to a low light intensity. In addition, the pH was lower in the rhizosphere of C. tenella after the P-deficient treatment and there was a lower Km in the uptake of H2PO4 - than for V. persica, suggesting that C. tenella could adapt to a low-P environment more easily. In conclusion, C. tenella growing together with V. persica had a competitive advantage under high-light-intensity and low-P-level conditions, but it lost its advantage with an increase in P fertilization and a decrease in light intensity.

Simvastatin attenuates delayed encephalopathy induced by carbon monoxide poisoning in rats by regulating oxidative stress, inflammation and NF-κB pathway

Chu Zhe,Liu Zuolong,Li Wei,Xu Dahai,Pang Li 대한독성 유전단백체 학회 2021 Molecular & cellular toxicology Vol.17 No.2

Background Carbon monoxide (CO) poisoning is a leading cause of morbidity and mortality worldwide. The delayed encephalopathy occurs a period after poisoning in patients without effective treatment. Simvastatin (Sim), a lipid-lowering drug, was reported to exert endothelial protective effects and inhibit inflammatory response. This research focused on the effects of Sim on delayed encephalopathy caused by CO poisoning. Objective The acute CO poisoning model was established by exposing rats to 2500 ppm CO gas for 40 min, then 3000 ppm for 30 min or until they lost consciousness. Rats in the treatment group were given Sim (20 mg/kg/day, ig.). The behavioral tests included the Morris water maze test and shuttle box. The pathological changes were evaluated by H and E staining. The inflammatory mediators were analyzed by ELISA. The expression levels of eNOS, iNOS and the NF-κB-related proteins were analyzed by Western blot. Results The results showed that Sim could alleviate CO-induced behavioral disorders and the hippocampal nerve cells apoptosis. Sim administration reversed the effects of CO on oxidative stress-related molecules. Sim could also inhibit the production of the inflammatory mediators induced by CO. The level of eNOS was decreased after CO exposure, while iNOS was increased. Sim could significantly inhibit the effects of CO. Furthermore, Sim inhibited the phosphorylation of IκBα (an NF-κB inhibitory protein), i.e., the activation of NF-κB, which indicated that Simvastatin reduced the inflammatory response induced by CO poisoning partially through inhibiting the activation of NF-κB signaling pathway. Conclusion To sum up, our research indicated that Sim could attenuate the delayed encephalopathy induced by CO poisoning via regulating oxidative stress, inflammation and NF-κB pathway. Background Carbon monoxide (CO) poisoning is a leading cause of morbidity and mortality worldwide. The delayed encephalopathy occurs a period after poisoning in patients without effective treatment. Simvastatin (Sim), a lipid-lowering drug, was reported to exert endothelial protective effects and inhibit inflammatory response. This research focused on the effects of Sim on delayed encephalopathy caused by CO poisoning. Objective The acute CO poisoning model was established by exposing rats to 2500 ppm CO gas for 40 min, then 3000 ppm for 30 min or until they lost consciousness. Rats in the treatment group were given Sim (20 mg/kg/day, ig.). The behavioral tests included the Morris water maze test and shuttle box. The pathological changes were evaluated by H and E staining. The inflammatory mediators were analyzed by ELISA. The expression levels of eNOS, iNOS and the NF-κB-related proteins were analyzed by Western blot. Results The results showed that Sim could alleviate CO-induced behavioral disorders and the hippocampal nerve cells apoptosis. Sim administration reversed the effects of CO on oxidative stress-related molecules. Sim could also inhibit the production of the inflammatory mediators induced by CO. The level of eNOS was decreased after CO exposure, while iNOS was increased. Sim could significantly inhibit the effects of CO. Furthermore, Sim inhibited the phosphorylation of IκBα (an NF-κB inhibitory protein), i.e., the activation of NF-κB, which indicated that Simvastatin reduced the inflammatory response induced by CO poisoning partially through inhibiting the activation of NF-κB signaling pathway. Conclusion To sum up, our research indicated that Sim could attenuate the delayed encephalopathy induced by CO poisoning via regulating oxidative stress, inflammation and NF-κB pathway.

Poster Presentations : Basic Research, Parenteral Nutrition ; The Use of Glycerol in Post-Operative Hypocaloric Peripheral Parenteral Nutrition

( Li Chu Sun ),( Ying Ling Shih ),( Chien Yu Lu ),( Jan Sing Hsieh ),( Jaw Yuan Wang ) 한국정맥경장영양학회 2005 The 11th PENSA Congress Vol.2005 No.-

Fuzzy system and Improved APIT (FIAPIT) combined range-free localization method for WSN

( Xiaofeng Li ),( Liangfeng Chen ),( Jianping Wang ),( Zhong Chu ),( Qiyue Li ),( Wei Sun ) 한국인터넷정보학회 2015 KSII Transactions on Internet and Information Syst Vol.9 No.7

Among numerous localization schemes proposed specifically for Wireless Sensor Network (WSN), the range-free localization algorithms based on the received signal strength indication (RSSI) have attracted considerable research interest for their simplicity and low cost. As a typical range-free algorithm, Approximate Point In Triangulation test (APIT) suffers from significant estimation errors due to its theoretical defects and RSSI inaccuracy. To address these problems, a novel localization method called FIAPIT, which is a combination of an improved APIT (IAPIT) and a fuzzy logic system, is proposed. The proposed IAPIT addresses the theoretical defects of APIT in near (it`s defined as a point adjacent to a sensor is closer to three vertexes of a triangle area where the sensor resides simultaneously) and far (the opposite case of the near case) cases partly. To compensate for negative effects of RSSI inaccuracy, a fuzzy system, whose logic inference is based on IAPIT, is applied. Finally, the sensor`s coordinates are estimated as the weighted average of centers of gravity (COGs) of triangles` intersection areas. Each COG has a different weight inferred by FIAPIT. Numerical simulations were performed to compare four algorithms with varying system parameters. The results show that IAPIT corrects the defects of APIT when adjacent nodes are enough, and FIAPIT is better than others when RSSI is inaccuracy.

Exploiting the Reversibility of GTBP1 Catalyzed One-pot Reactions for the Synergistical Synthesis of Ponasterone A and Phenolic Glycosides

Yuqiang Li,Bingfeng Li,Tianyi Chen,Bin Wu,Jianlin Chu,Bingfang He 한국생물공학회 2021 Biotechnology and Bioprocess Engineering Vol.26 No.3

In the process of glycosyltransferase-catalyzed transglycosylation, the synthesis of uridine diphosphate sugars is generally thermodynamically disfavored. This study found that GTBP1 from Bacillus pumilus BF1 displayed the potential to reversely synthesize uridine diphosphate glucose (UDPG, UDP-glucose), which catalyzed the deglycosylation of ponasteroside A and the transglycosylation of phenolic compounds in one-pot reaction. Sequence alignment and phylogenetic tree analysis of GTBP1 and other GTs with reversible glycosylation ability were also implemented. Using solvent engineering strategy, the reaction time in water-organic biphasic was shortened and the conversion was improved. The final conversion of ponasterone A was reached 93.4%, and the final yield of ferulic acid glycoside was 92.7%. Using the fed-batch technology with the coupled reaction, the cumulative product of ponasterone A was about 1.97 g/L, and the level of produced ferulic acid glycoside was about 1.52 g/L. The substrate specificity of the GTBP1 was also confirmed. It implies the diversity of GTBP1's ability to construct UDP cycles.