추황배(Pyrus pyrifolia Nakai cv. Chuhwangbae) 과피로부터 1종의 Sterol과 3종의 배당체 화합물의 단리·동정

이유건(Yu Geon Lee1),조정용(Jeong-Yong Cho),이현주(Hyun Joo Lee),이용현(Yong Hyun Lee),이상현(Sang-Hyun Lee),한태호(Tae-Ho Han),김월수(Wol-Soo Kim),박근형(Keun-Hyung Park),문제학(Jae-Hak Moon) 한국식품과학회 2013 한국식품과학회지 Vol.45 No.5

본 연구에서는 배의 유용성 증명을 위한 일환으로 배의 화학성분을 분자수준에서 밝히고자 하였다. 이에 배 과피 MeOH 추출물을 용매분획하여 얻은 EtOAc-산성 분획과 EtOAc-중성분획을 대상으로 Sephadex LH-20, silica gel, 그리고 ODS colmn chromatography와 HPLC를 이용하여 정제 및 단리하였다. 그 결과, EtOAc-산성 분획과 EtOAc-중성 분획으로부터 각각 2종씩의 화합물을 단리하였다. 단리된 화합물 1-4는 1H- 및 13C-NMR 분석을 통하여 각각 (S)-(+)-2-cis-abscisic acid O-β-D-glucopyranosyl ester (화합물 1), 1-[4-O-β-D-glucopyranosyl]phenyl ethanone(piceoside, 화합물 2), β-sitosterol (화합물 3), 그리고 β-sitosteryl 3-O-β-D-glucopyranoside (화합물 4)로 동정되었다. 단리된 3종의 배당체 화합물(화합물 1, 2, 4)들은 본 연구에 의해 배로부터 처음 동정되었으며, 화합물 3은 추황배로부터 처음 동정되었다. 본 연구결과가 배 함유 성분연구는 물론 배의 기능성 해명 연구에도 추후 중요한 기초자료로 활용되길 기대한다. We isolated and identified antioxidants from acidic and neutral ethyl acetate fractions of the peel of pear (Pyrus pyrifolia N. cv. Chuhwangbae). We isolated 4 compounds from the methanol extract, by using 3 different types of column chromatography (Sephadex LH-20, silica gel, and octadecylsilane) and preparative HPLC. We identified the isolated compounds as (S)-(+)-2-cis-abscisic acid O-β-D-glucopyranosyl ester (compound 1), 1-[4-O-β-D-glucopyranosyl]phenyl ethanone (picroside, compound 2), β-sitosterol (compound 3), and β-sitosteryl 3-O-β-D-glucopyranoside (compound 4) by nuclear magnetic resonance analysis. We are the first to report the identification of compounds 1, 2, and 4 from pear.

정상인에서 부분적 수면박탈이 수면구조에 미치는 영향

왕성근,이정규,이충숙,최하석,유남재,정인형,이선우 충남대학교 의과대학 지역사회의학연구소 1992 충남의대잡지 Vol.19 No.2

To identify the effects of partial sleep deprivation on the sleep structure, the authors performed polysomnographic study for 9 healthy young adults. The subjects were college students, ranging in age from 20 to 25 years, without personal past or present histories of medical, neurological, or psychiatric illness and suffering from current sleep disturbance or parasomomnias. Sleep records were analyzed according to the criteria of Rechtschaffen and Kales' manual and tried paired t-test. The results were summarized as follows. 1. As for sleep parameters, total sleep time(TST), REM latency and REM duration were not changed significantly during deprivation period and recovery period compared with baseline. Sleep efficiency increased significantly during deprivation period and recovery period compaired with baseline, and sleep latency and WASO were shortened significantly during deprivation period and recovery period compared with baseline. 2. As for sleep structure, 1) The absolute value(minutes) of stage 1 decreased significantly during deprivation period and first recovery night compared with baseline, and the relative value(percentage) of stage 1 decreased significantly during deprivation period and recovery period compared with baseline. 2) The absolute value(minutes) of stage 2 decreased significantly during deprivation period compared with baseline, and the relative value(percentage) of stage 2 decreased significantly during deprivation period and first recovery night compared with baseline. 3) The absolute value(minutes) of slow wave sleep were not changed significantly during deprivation period and recovery period compared with baseline, and the relative value(percentage) of slow wave sleep increased significantly during recovery period compared with baseline. 4) The absolute value(minutes) of REM sleep decreased significantly during deprivation period compared with baseline, and the relative value(percentage) of REM sleep decreased significantly in the first deprivation night but, were not changed significantly during recovery period compared with baseline.

관상동맥 질환에서 SDF-1α의 농도

김보영,박용규,박형서,노상필,정승현,이유선,이정우,이재환,최시완,정진옥,성인환 충남대학교 의과대학 의학연구소 2003 충남의대잡지 Vol.30 No.1

Atherosclerosis is now viewed as an inflammatory disease of the vascular system. Expression of several chemokines, including monocyte chemoattractant protein(MCP)-1, MCP-4, RANTES(regulated on activation normal T-cell expressed and secreted), and interleukin-8(IL-8) are increased in human atherosclerotic plaques compared with normal vessels. They are involved in the pathogenesis of atherosclerosis and plaque rupture by activating and directing leukocytes into the atherosclerotic lesions. However, some are involved in homeostatic functions such as normal leukocyte traffic and growth regulation. SDF-la is a multi-functional cytokine that is involved in myelogenesis, hematopoiesis, angiogenesis and injured gastric mucosal regeneration in the gastric ulcer patient. SDF-la is recently shown to be highly expressed in atherosclerotic plaques and a potent platelet agonist. At least in high concentrations, SDF-la may mediate antiinflammatory and matrix stabilizing effects in unstable angina. Many studies are going on to know the function of SDF-la in coronary artery diseases. I investigated the difference of the plasma level of SDF-la between control group and coronary artery disease group. Total 75 subjects were enrolled. The diagnosis of coronary artery disease was confirmed in all patients by coronary angiography. Control subjects in this study were confirmed normal by coronary angiography. Clinical profile and risk factors were also reviewed. Control subjects in this study were 27 (M=10, F=17). Plasma for the study was collected before the angiography and centrifuged. SDF-la analysis was performed by ELISA. Plasma level of SDF-la is significantly increased in patients with stable angina(n=20) and unstable angina group(n=28) compared with healthy control group(n=27). the risk factors do not influence the plasma level of SDF-la in coronary artery diseases. In this study, plasma level of SDF-la is increased in patients with stable angina and unstable angina groups compared with healthy control group(P<0.05). the risk factors do not influence the plasma level of SDF-la in coronary artery diseases.

혈액투석 환자들의 식염인지도와 식품섭취에 관한 연구

유정아,이용욱,김종규 대한보건협회 2000 대한보건연구 Vol.26 No.1

This study was performed to investigate the perception of salt and food intake of the outpatients with hemodialysis and to evaluate the relationship between dietary survey assessment and blood biochemical data. It was based on a nutrient analysis of food intake records and blood analysis collected from 40 hemodialysis outpatients (male 24, female 16) of a hospital in Seoul from June to October 1998. Each of the subjects provided a 24-hour recall of food intake on the previous day, frequency of consumption, and attitude about diet therapy to a trained interviewer. The subjects' salt solution preference was tested by sensory evaluation and fasting blood samples from the subjects were analyzed by a biochemicalanalyzer. Among several dietary therapy methods, most preferred limiting salt intake. Among the four basic flavors: sweet, salty, sour, and bitter, and spicy hot flavor, they preferred salty and spicy hot foods. The subjects' preferred concentration of salt solution was in the order of 0.50%>0.25%>0.75%, similar to the normal population as others have reported. Food intake frequency showed that the subjects frequently eat cereals and vegetables (2∼3 times per day), and oils and candies (1 time per day). They eat meats, fishes, eggs, legumes, milk and fruits moderately (2∼3 times per week). They eat less often potatoes, bread & biscuits (1 time per week), and meat products and salt-fermented fishes & salted vegetables (2∼3 times per month) and they rarely eat nuts. The nutrient intake of the subjects was not satisfactory, especially the energy and protein intakes were much lower than the required amounts for the subjects. Also the calcium, phosphorus, and vitamin A, B_1, B_2 and niacin intakes did not meet the recommended dietary allowance of Koreans. The sodium intake was at a normal range. In the results of the blood analysis, the hemoglobin and hematocrit levels of the subjects were much lower than the normal range. Total protein, albumin, total cholesterol, and sodium levels were at normal range. Uric acid and potassium levels were at an acceptable range. However, blood urea nitrogen, creatinine, calcium and phosphorus levels were higher than the normal range. There was a significant correlation between the preference of salt solution and sodium intake in the subjects (p<0.05). A significant negative correlation between calcium intake and hemoglobin, between iron intake and serum potassium or creatinine, between protein intake and hemoglobin or hematocrit, and between vitamin B_2 intake and hematocrit were observed (p<0.05). A significant positive correlation was found between potassium intake and blood urea nitrogen (p<0.05). It seemed that the patients did not get enough information about the therapeutic diet from registered dietitians. Because of anorexia and poor intake of nutrients, most of the patients had energy and protein malnutrition. The serum sodium level of the subjects were acceptable, therefore limitation of sodium intake level which may cause the anorexia may not be necessary. These hemodialysis patients need education, adequate diet therapy, and energy rich foods based on their individual biochemical data.

광주전남지역 정상 신생아의 코의 형태와 계측치에 대한 연구

이유진,양정열,이승찬,천지선,박상기 朝鮮大學校 附設 醫學硏究所 2003 The Medical Journal of Chosun University Vol.28 No.2

Background and Objectives: Cleft lip and palate are common defects in congenital deformity. They are the most frequent deformity in the face. Nose deformity is most common among various facial deformity at the center of face. So we must operate to correct the deformity at the earliest stage to give a patient and parent spiritual relief from anxiety, and it is the trend of the treatment recently. We must know the facial anthropometry in newborn to try at the early stage, but there is a lack of literature and research on newborns. So we carried out this research to form base data for operations. Materials and Methods : The noses of 90 newborns in Gwangju-Cheonnam area were analyzed quantitatively, based on 10 nasal measuring points and 13 anthropometric values taken directly from the face of newborns. The relationship between the nasal measurements were studied in 11 proportion indices. Results : Following are our mean values in newborn. Width of the nasal root was 13. 9mm. Width of the nose was 22. lrnm, Width of the columella was 4. 5mm. Height of the nose was 22.6mm. Length of the nasal bridge was 20.1mm. Nasal tip protrusion was 8.9mm. Width of the nostril floor was 6.4mm. Thickness of the nasal ala and length of the nasal ala were 4.0mm, 15.4mm. Length of the columella was 3.9mm. The most common nostril shape was Type II (0-45 degrees in inclination of the nostril axis from the horizontal). Nasal index was 97.8. Columella-nasal tip protrusion length index was 43.9. Conclusion : The development of the columella was relatively incomplete in the case of newborn and was the result to appear. The normal indicies could be used clinically in identifying the it's normality as well as morphologic characteristics of the craniofacial structures. They also could be used in differential diagnosis between the morphologically similar congenital craniofacial anomalies. In preoperative design, in setting the criteria of the postoperative results, and assessing the effect of the operation, Finally, they supply the plastic surgeon and the other specialists in the craniofacial surgery with basic normal population data.

이산선택모형을 이용한 주거용수용가의 전력서비스 전환비용 추정

이종수,이동헌,이정동,박유리 한국환경경제학회 한국자원경제학회 2004 자원·환경경제연구 Vol.13 No.2

전력산업은 필수재적인 성격과 막대한 초기시설투자의 필요 등으로 독점적 시장구조를 갖고 있는 것이 일반적이었다. 그러나 기술의 진보로 인해 전력산업과 같은 네트워크 산업에서의 규모의 경제로 인한 효과가 감소되면서 자연독점적 산업에서의 경쟁도입이 점차 확산되고 있는 것이 세계적인 추세이다. 우리나라에서도 현재 전력산업 구조개편이 추진되고 있지만 아직 소매시장에서의 경쟁 도입은 이루어지지 않은 상황이다. 소매경쟁은 구조개편의 성과를 확인할 수 있는 완성단계로서 소매시장에서의 경쟁이 효율적으로 일어나는 가의 문제는 구조개편을 추진하는 정책결정자의 입장에서 중요한 문제이나 이에 대한 정량적인 연구는 거의 없는 현실이다. 본 논문은 전력소매시장에서 경쟁의 유효성을 전환비용을 통해 정량적으로 분석하였다. 전환비용이 크면 시장에 경쟁이 도입되더라도 실질적으로 소비자가 기존 사업자에게 고착 될 가능성이 높으므로 전환비용의 크기를 정량적으로 측정하는 것은 향후 전력소매시장에 도입된 경쟁이 유의하게 작용할 것인가에 대해 정보를 제공할 수 있다. 이를 위해 본 논문에서는 컨조인트 분석을 통해 얻어진 가상적인 상황에서의 소비자의 전력상품에 대한 진술선호 자료를 바탕으로 전력소매시장에서의 전환비용을 정량적으로 추정하였다. 실증분석 결과, 전력상품의 특성상 절차상의 전환비용은 크지 않았으나 기존 공급업자에 대한 상대적인 브랜드 로열티가 크게 나타났으며, 전력상품의 선택시 소비자는 가격을 가장 중요한 요인으로 고려하고 있는 것으로 나타났다 이 결과는 영국 등의 해외 소매경쟁 자료와 유사한 것이며, 이를 통해 신규 진입기업의 경영효율성에 따른 가격경쟁력과 소비자의 전환가능성의 관계 파악이 가능하므로 개별기업이나 정책결정자에게 소매경쟁 진출 및 도입의 판단근거를 제시할 수 있을 것이다. Generally, electricity market has monopoly market structure because of need of enormous investment for infrastructure. However, the introduction of competition in network industry as electricity is a tendency of the world with decreasing the effects of economy of scale due to the advancement of technology. Now, electricity industry restructuring is in progress but the competition in electricity retail market is not in force yet in Korea. Whether a effective competition exist or not is very important to policy decision maker who drive restructuring, but there are small numbers of quantitative researches on that. In this study, we estimated the effectiveness of competition in the electricity retail market through switching costs. If switching costs are high, consumers actually can be locked in incumbent firm in spite of introduction of competition. Therefore switching is a critical factor to determine effectiveness of competition and to estimate the size of switching costs quantitatively can proffer the information about whether the competition in the electricity retail market is effective or not in the future. We estimated switching costs using consumer' stated-preference data by conjoint analysis. In according to estimation results, the cost of switching process is not so high, but the relative brand loyalty of an incumbent company is significantly high. And the price is considered as the most important factor choosing an electric service commodity. Based on the empirical results, it is possible to analyze the relationship between suppliers' competitiveness resulted from management efficiency and customers' switching possibilities. The paper therefore provides guidance for suppliers in deciding to enter into retail competition and for policy makers in introducing retail competition. And it has a significance of estimating the switching costs directly.

ILP 프로세서에서 성능향상을 위한 조건실행에 관한 연구

이상정,김유신 순천향대학교 부설 산업기술연구소 1998 순천향 산업기술연구소논문집 Vol.3 No.3

In this paper, predicated execution and its effect for improving performance in ILP processors are studied. Predicated execution, which represents through if-conversion that conditionally executes parts of control flow graphs, transforms control dependency into data dependency. The larger basic block after if-conversion generally contains more amounts of parallelism, permitting the compiler to schedule instructions that are more parallel and use the processor resources better. To evaluate this, it is developed the simulator which supports predicated execution. Using the simulator, the performance effects of predicated execution are analyzed. The results show that the scheme with predicated execution increases performance by 21% over the scheme without it.

외상 환자에서 의식소실 유무와 외상후 스트레스 장애의 유병율, 증상 양상, 우울 및 불안에 관한 연구

이유진,연규월,우행원,김영철,임원정 大韓神經精神醫學會 2002 신경정신의학 Vol.41 No.4

연구목적 : 본 연구에서는 의식 소실 유무에 따른 외상후 스트레스 장애 유병율 및 증상, 우울, 불안 증상을 알아보기 위해 외상을 당한 환자를 의식 소실이 있었던 환자군과 의식 소실이 없었던 환자군으로 구분하여 두 집단의 외상후 스트레스 장애의 유병율, 증상 및 불안 정도를 비교해보고자 하였다. 방 법 : 사고를 당하고 1달 이상 경과한 환자 120명(남자 69명, 여자 51명)을 대상으로 하였다. 대상군을 의식 소실 유무에 따라 소실군과 비소실군으로 구분하고 사회인구학적 변수, 사고 당시 의식 소실의 유무, 의식 소실 시간, 사고의 종류, 사고에 대한 기억, 사고의 심각도, 손상 심각도 점수(Injury Severity Score)를 조사하여비교하였다. 또한 외상후 스트레스 장애 척도(Clinician Adminstered PTSD Scale), Beck의 우울증 척도(Beck Depression Inventory), 상태 특성 불안처도- Ⅰ, Ⅱ(State Trait Anxiety Inventory- Ⅰ, Ⅱ)를 이용하여 조사하여 두 군 사이의 외상후 스트레스 장애유병율 및 증상, 우울, 불안 정도를 비교하였다. 결 과 : 통 연구 대상자 120명중 의식 소실이 있었던 환자군(소실군)은 56명(46.7%)이었고 의식 소실이 없었던 환자군(비소실군)은 64명(53.3%)이었다. 총 대상자 120명중 30명(25%)이 외상후 스트레스 장애로 진단되었으며 소실군 56명중 17명(30.4%)이 비소실군 64명중 13명(20.3%0이 각각 외상후 스트레스 장애로 진단되었으나 두 군간의 차이는 통계적으로 유의하지 않았다(x^2=1.607, P>0.05). 외상후 스트레스 장애 증상 척도에서는 소실군에서 사고에 대한 기억 상실, 흥미 상실, 이탈, 감정 둔마, 수면자애, 예민함, 집중력 감소 증상의 빈도가 유의하게 높았다(P<0.05). 비소실군에서느 반복적인 회상과 놀람 반응 빈도가 유의하게 높았다(p<0.05). Beck의 우울증 척도 점수는 소실군에서 38.8±8.8, 비소실군에서 31.0±7.9로 의식 소실을 보고한 환자군의 점수가 유의하게 높았으며(t=-3.16, 0<0.05) 상태 특성 불안 척도-I, II는 소실군에서 각각 44.5±3.3, 44.6±3.9, 비소실군에서 각각 42.9±3.0, 42.3±3.4로 소실군의 점수가 유의하게 높았다(t=-2.75, p<0.05, t=-3.38, p<0.05). 결 론 : 사고 당시 의식 소실이 없었던 환자군과 비교하여 외상후 스트레스장애가 비슷한 빈도로 진단되며 의식 소실이 외상후 스트레스 장애의 발생에 유의한 영향을 미침은 물론 불안 및 우울에도 유의한 영향을 미친다고 할 수 있다. Objectives :This study investigated PTSD prevalence, symptoms, depressed mood and anxiety, comparing two groups of the patients in trauma ; one who had experienced the loss of consciousness(group "A" here after) and the other group of patients who had not(group "B" here after). Method : subjects were 120 patients(age18-66) who had received trauma(traffic accident, fall down) more than 1 month. Before they consisted of 56 patients who had experienced the loss of the consciousness(46.7%) and 64 who had not(53.3%). Men were 69 and women were 51. Clinician administered PTSD Scale, Beck Depression Inventory, State Trait Anxiety Inventory-I, II were administered. The factors such as sociodemographic variables, unconsciousness at the time of trauma, memory about tranmatic accidents were considered. Result : Out of 120 subjects, 30 patients(25%) were diagnosed as PTSD : 17 from 56(30.4%) and 13 from 64(20.3%) were found in the group "A" and "B" respectively. Prevalence of PTSD was higher in the group "A" showed significant difference between two groups(x^2=1.607,p>0.05). The CAPS of group "A" showed significantly high frequency in the loss of interest, detachment, restricted affect, sleep difficulty, irritability/anger. concentration difficulty(p<0.05), while the group "B" scored high in intrusive recall and increased startle. The scores of BDI, STAI-I, II were significantly higher in the group "A"(t=-3.16, p<0.05)(t=-2.75, p<0.05, t=-3.38, p<0.05). Conclusion : PTSD was mre frequent and depressed mood and anxiety appeared more often in the group qho experienced the loss of consciousness. Thus the loss of the consciousness at the time of trauma prones of the aevelopment PTSD and symptom of aepression and anxiety.

방위에 따른 속리산과 오대산의 산림군락 구조분석

유재은,이정호,권기원 명지대학교 자연과학연구소 2004 자연과학논문집 Vol.23 No.-

본 연구는 방위에 따른 속리산 및 오대산 삼림식생을 분석하고자 하였다. 속리산 조사구와 오대산 조사구를 방위에 따라 출현하는 주요 종의 중요치를 살펴보면 속리산의 경우 북향에서는 쇠물푸레, 벚나무, 개옻나무, 굴피나무, 참회나무 등 8종에 중요치가 높게 분포하고 있음을 볼 수 있고, 동향에서는 소나무, 고로쇠나무, 산초나무, 소태나무, 작살나무 등 6종이, 남향에서는 굴참나무, 졸참나무, 생강나무, 물푸레나무, 짝자래나무 등 13종이, 서향에서는 신갈나무, 당단풍, 쪽동백나무, 노린재나무, 철쭉 등 13종에 중요치가 높게 출현한다. 오대산의 경우를 보면 북향에서는 당단풍, 까치박달, 거제수나무, 음나무, 잣나무등 18종이 중요치가 높게 분포하고 있음을 볼 수 있고, 동향에서는 전나무, 소나무, 느릅나무, 산벚나무, 들메나무 등 5종이, 남향에서는 신간나무, 물푸레나무, 철쭉, 진달래, 개벚지나무 등 7종이, 서향에서는 피나무, 고로쇠나무, 난티나무, 쪽동백나무, 황벽나무 등 10종이 중요치가 높게 출현함을 알 수 있다. 이러한 결과로 볼 때 속리산에서는 남향과 서향이 각각 13종씩 26종으로 북향과 동향의 14종 보다 배에 가까운 많은 수의 나무들이 중요치가 높게 출현하고 있는 것에 반하여, 오대산에서는 북향과 동향이 각각 18종과 5종씩 23종으로 남향과 서향의 17종 보다 월등히 많게 출현함을 알 수 있다. 이와 같은 결과는 속리산과 오대산이 정반대의 현상으로 속리산이 해발고가 낮고 난온대성 수종의 우위를 점하는 것에 비하여 오대산은 해발고가 높고 냉온대성 수종이 우위를 차지하고 있는데서 오는 현상으로 보여진다. This study was to research environment of the forest vegetation structure according to the directions in Mt. Sokri and Odae. According to direction, the importance value of 8 species of Fraxinus sieboldiana, Prunus serrulate var. spontanea, Rhus trichocarpa ect. appeared highly on the north, 6 species of Pinus densiflora, Acer mono, Zanthoxylum schinifolium ect. highly appeared on the east, 13 species of Quercus variabilis, Quercus serrata, Lindera obtusiloba ect. highly appeared on the south, 13 species of Quercus mongolica, Acer pseudo-sieboldianum, styrax obassia ect. highly appeared appreared on the west in Mt. Sokri. The importance value of 18 species of Acer pseudo-sieboldianum, Carpinus cordata, Betula costata ect. highly appeared on the north, 5 species of Abies holophylla, Pinus densiflora, Ulmus dabidiana var. japonica ect. highly appeared on the east, 7 species of Quercus on the south, 10 species of Tilia amurensis, Acer mono, Ulmus laciniata ect. highly appeared on the west in Mt. Odae

무전해 Ni-P 도금시의 저전류밀도의 영향

정유엽,이성룡,황용길 동아대학교 공과대학 부설 한국자원개발연구소 1988 硏究報告 Vol.12 No.2

This study has been carried out to investigate the effect of low current in electroless Ni-P plating. The results obtained from the experiment are summarized as follows ; 1) The plating rate in the composite plating of electrolysis and electroless is more double than in electroless plating. And optimum temperature of electroless plating is 85-95℃ but composite plating has been gained a excellent quality of plating at 40℃ 2) The higher temperature, higher PH, and lower current density, the better a leveling of plating surface is. And the composite plating under 0.5A/d㎡ current density is better than the electroless plating in the leveling of plating surface. 3) With passing the time, the composite plating is less than that of the electroless in the reduction of plating rate. 4) Composite plating is higher than electroless plating in hardness when they have been plated and heat-treated at 350℃