백서 간재생중 H2B Histone 유전자 발현에 대한 Octamer Binding Transcription Factor의 역할

임규,방재웅,최두식,손미영,이영철,박청,김태동,김영래,박종일,윤완희,박승길,황병두 한국노화학회 2001 한국노화학회지 Vol.11 No.1

12-O-Tetradecanoylphorbol-13-Acetate에 의한 HL-60 세포 분화유도중 Vimentin 유전자 전사조절에 대한 AP-1의 역할

임규,김진희,권도원,김승민,이명선,윤경아,손미영,박종일,윤완희,황병두 충남대학교 생물공학연구소 1999 생물공학연구지 Vol.7 No.-

Purpose: To gain insight on the role of AP-1 in transcriptional regulation of vimentin gene during differentiation of HL-60 cells by 12-O-tetradecanoylphorbol-13-acetate (TPA), the levels of vimentin mRNA and AP-1 have been investigated with Northern blot hybridization and DNA mobility shift assay. Materials and Methods: HL-60 cells were grown in RPMI 1640 medium supplemented with 10% heat-inactivated fetal calf serum and antibiotics in a humidified 5% CO_2 at 37℃. Total RNA was prepared by a modification of the method of Karlinsey et al. Northern blot hybridization was performed by the method of Virca et al. EcoRI fragment of pVIM-GEM was used as probe for vimentin mRNA. DNA mobility shift assay was performed by the method of Lim et al. End labeled DNA probe(Upper strand, 5'-CGCTTGATGAGTCAGCCG- 3') for AP-1 binding activity was mixed with nuclear extracts in a 20 μl reaction volume containing 300 mM KCI, 60 mM HEPES, pH 7.9, 25 mM MgCI_2, 1 mM EDTA, 1 mM DTT, 60% glycerol, and 2 ㎍ of poly[dI-dC]. Results: TPA increased vimentin mRNA levels, with maximal stimulation reached at 24 hr. The level of vimentin mRNA was induced in proportion to the concentration of TPA. TPA-induced vimentin mRNA was almost reduced by actinomycin-D pretreatment. TPA-induced stimulation of vimentin gene was completely reduced by staurosporin pretreatment. In DNA mobility shift assay, AP-1 newly appeared at 24 hr during TPA-induced differentiation and was almost not detected after the pretreatment of staurosporin. Conclusions: These results suggest that the induction of vimentin mRNA during TPA-dependent differentiation in HL-60 cells may be mediated by protein kinases C signal transduction and AP-1 is important to transcriptional regulation.

12-O-Tetradecanoylphorbol-13-Acetate에 의한 HL-60 세포 분화유도중 Vimentin 유전자 전사조절에 대한 AP-1의 역할

임규,김진희,권도원,김승민,이명선,윤경아,손미영,박종일,윤완희,황병두 忠南大學校 癌共同硏究所 1998 癌共同硏究所 硏究誌 Vol.2 No.1

Purpose: To gain insight on the role of AP-1 in transcriptional regulation of vimentin gene during differentiation of HL-60 cells by 12-O-tetradecanoylphorbol-13-acetate (TPA), the levels of vimentin mRNA and AP-1 have been investigated with Northern blot hybridization and DNA mobility shift assay. Materials and Methods: HL-60 cells were grown in RPMI 1640 medium supplemented with 10% heat-inactivated fetal calf serum and antibiotics in a humidified 5% CO_(2) at 37°C. Total RNA was prepared by a modification of the method of Karlinsey et al. Northern blot hybridization was performed by the method of Virca et al. EcoRI fragment of pVIM-GEM was used as probe for vimentin mRNA. DNA mobility shift assay was performed by the method of Lim et al. End labeled DNA probe(Upper strand, 5'-CGCTTGATGAGTCAGCCG- 3') for AP-1 binding activity was mixed with nuclear extracts in a 20 μl reaction volume containing 300 mM KC1, 60 mM HEPES, pH 7.9, 25 mM MgCl_(2), 1 mM EDTA, 1 mM DTT, 60% glycerol, and 2μg of poly[dI-dC]. Results: TPA increased vimentin mRNA levels, with maximal stimulation reached at 24 hr. The level of vimentin mRNA was induced in proportion to the concentration of TPA. TPA-induced vimentin mRNA was almost reduced by actinomycin-D pretreatment. TPA-induced stimulation of vimentin gene was completely reduced by staurosporin pretreatment. In DNA mobility shift assay, AP-1 newly appeared at 24 hr during TPA-induced differentiation and was almost not detected after the pretreatment of staurosporin. Conclusions: These results suggest that the induction of vimentin mRNA during TPA-dependent differentiation in HL-60 cells may be mediated by protein kinases C signal transduction and AP-1 is important to transcriptional regulation.

소비자 문제에 대한 대응행동 : 여자대학생을 중심으로

임정빈,이영호,조미환,강은주 漢陽大學校 韓國生活科學硏究所 2000 韓國 生活 科學 硏究 Vol.- No.18

The purpose of this study was to provide with the basic research to aid to establish in the direction of consumer education, which investigate the actual condition of consumer complaining behavior according to consumer problems. Content analysis method was used and the sample 624 case was taken from report about experienced according to consumer problems by university of women. The major results are as follows: They have much more consumer complaining behavior, when they experience the problem about non-durable goods, and less experience them in case about durable goods. But they need to knowledge(information) about the durable goods and a way of settlement when they experience problems after service and structural problems.

흉선종 : 외과적으로 절제된 6예 분석 Muller-Hermelink 분류체계를 중심으로 Analysis of 6 surgically excised cases Centered to Muller-Hermelink Classification

오미혜,임병성,김미영,오삼세 世宗醫學硏究所 2000 世宗醫學 Vol.17 No.1

산채류의보존과 일본식 절임

오영애,하귀현,박인경,김미향,김미정,김미경,정자림,이명숙,김순동 대구효성가톨릭대학교 식품과학연구소 1992 식품과학지 Vol.4 No.-

This paper showed the summarization of the preservative method of wild vegetables in the house and the precessing method of Japanese pickles. The preservative methods of wild vegetables were explained devided into long, short term, fresh state, heat treatment and drying. Salt, soybean fermented sauce, soybean fermented paste, lees of refined rice wine and residue in the preparation of bean curd were used as the materials of Japanese pickles.

전시공간디자인의 시지각적 Sequence구조에 관한 연구

황미영,임채진 대한건축학회 2003 대한건축학회논문집 Vol.19 No.12

This research is focusing on comparing the characteristics of selected museums with analyzing indicators which form the exhibition space's sequence. The analysis is based on measuring the co-relations between physical structure system and the visual-perception sequence structure relating with viewer's movement. The results of this research are below; First, there are effective Variables in forming sequence system of visual perception. They are permeability, direction, visibility, observation value and scenario justified map. Among the effective variables, permeability, direction and observation value are directly effective main factors, and the rest the variables are partially effective subsidiary factors. Second, the variables form the system of visual perception not by themselves but by complicating effect the environment of show. Also they are forming partially different system of visual perception according to the different museums.

Space Syntax에 의한 展示디자인의 空間分化 및 Remodeling方法에 관한 硏究

黃美英,林采震 홍익대학교환경개발연구원 1999 환경개발연구논문집 Vol.4 No.-

The purpose of this study is to clarify the relationship between the exhibits layout and the spatial organization in exhibition spaces. As an analytical method, the space syntax theory devised by Bill Hillier(1984) was applied for analyzing the potential differentiation of exhibition spaces and remodeling method in Seoul City Museum selected for case study. The results are as following. Especially from the convex analysis, axial analysis and exhibits(interpretation); 1)the closed spatial system(1F) has high visibility and Intelligibility in hall area but lacks route continuity, 2)the circulated spatial system(2F) has low global visibility but offers high intelligibility and connectivity due to its coercive circulation, 3)and the organizaton of exhibition design consists in differentiating space reintegrates them into another spatial order. Therefore, these process are expected to provide a methodological framework for analysing and interpreting space organizations of design, leading to the clear explanation of remodeling.

보통형 간질성 폐렴 : 방사선 및 병리학적 소견 분석 Radiologic and Pathologic Analysis

오미혜,김미영,임병성,김태형,오삼세 世宗醫學硏究所 2000 世宗醫學 Vol.17 No.2

감과실의 연화에 따른 물성 변화와 헤미셀루로즈의 변화

김순동,임영숙,조성경,김미향 대구효성가톨릭대학교 식품과학연구소 1992 식품과학지 Vol.4 No.-

감의 연화에 따른 물성 변화와 이에 따른 세포벽 다당류의 조성과 함량 및 column chromatography를 통한 계략적인 분자량의 변화를 조사하였다. 조세포벽의 함량은 연화된 감에서 현저히 감소되었다. 또한 온도변화에 따른 감의 물성변화와 세포벽 다당류의 변화를 조사하였다. 그 결과 25℃에서 가장 쉽게 연화 되었으며 45℃에서는 연화가 억제되었다. 연화가 진행되는 동안에 부착성은 증가하였으나 경도는 감소되었다. 이러한 현상은 25℃에서 가장 현저하게 나타났다. 조세포벽의 함량은 연화된 감에서 현저히 감소되었은데 구체적으로 ionically associated pectin(IAP)은 59%, covalently bounded pectin(CBP)은 60%, hemicellulose fraction 2(HF2)는 74%가 감소된 반면 hemicellulose fraction 1(HF1)과 cellulose fraction(CF)은 큰 변화가 없었다. 연화된 감의 IAP, CBP에서는 pentose와 hexose의 감소율이 높았다. HF1과 HF2역시 uronic acid의 함유율이 높았는데 HF2가 현저하였다. 또 연화에 따라 HF1은 pentose와 HF2는 uronic acid와 hexose의 손실율이 높았다. 특히 HF2는 수백만 단위의 거대분자가 만단위까지 저분자화 하였다. 이상의 결과에서의 감의 연화는 CBP에 연결된 hemicellulose중 특히 HF2의 저분자화와 밀접한 관련이 있는 것으로 판단 되었다. The changes of cell wall polysaccharides were studies by gel filteration chromatography. Various cell wall polysaccharides such as ionically associated pectin(IAP), covalently bounded pectin(CBP), 2N potasium hydroxide soluble hemicellulosic fraction(HF1) and 0-3N potasium hydroxide soluble hemicellulosic fraction(HF2) were fractionated by chemical method. The content of crude cell wall remarkably decreased in the soft persimmon. The changes of texture, composition of cell wall polysaccharides during preserving of persimmon at various temperature were estimated to investigate the softening characteristics of persimmon fruit. The softening of persimmon was the most promoted at 25℃, Whereas it was inhibited at 45℃. During softening adhesiveness increased and hardness decreased. This phenomenon was obvious at 25℃. The decreasing rates of IAP, CBP and HF2 were 59, 60 and 74%, respectively, while HF1 and cellulose changed only a little during softening. The loss rate of pentose in IAP, of hexose in CBP, of hexose and uronic acid in HF2, of pentose in HF1 increased during softening. Though apparent molecular weight of all polysaccharides shifted from high molecular weight to low molecular weight polymer, the shifting degree of CBP and HF2 was especially remarkable during softening. It is suggested that the servere softening phenomenon of persimmon involved the degradation and dissolution of wall bounded-CBP and HF2 which were associated with each other.