Quantitative study of kinetics of neuron-degenerative related protein oligomerization

Yanjing Wang,Chi Wu 한국고분자학회 2016 한국고분자학회 학술대회 연구논문 초록집 Vol.41 No.2

Transmit Precoder Design for Two-User Broadcast Channel with Statistical and Delayed CSIT

( Yanjing Sun ),( Shu Zhou ),( Qi Cao ),( Yanfen Wang ),( Wen Liu ),( Xiaoguang Zhang ) 한국인터넷정보학회 2018 KSII Transactions on Internet and Information Syst Vol.12 No.5

Recent studies have revealed the efficacy of incorporating delayed channel state information at transmit side (CSIT) in transmission scheme design. This paper focuses on transmit precoder design to maximize the ergodic sum-rate in a two-user Multiple-Input Single-Output (MISO) system with delayed and statistical CSIT. A new transmit strategy which precodes signals in all transmit slots is proposed in this paper, denoted as all time-slots precoding Alternative MAT (AAMAT). There is a common procedure in conventional delayed-CSIT based schemes, which is retransmitting the overheard interferences. Since the retransmitting signal is intended to both users, all previous schemes tend to use only one antenna. We however figure out an improvement in spectral efficiency could be realized if all antennas can be utilized. In this paper, we detail the design of the procoder which enabling all antennas and also we compute a lower bound of the ergodic sum-rate in an ideal condition. In addition, simulation results demonstrate the superiority of our proposed scheme.

Nattokinase Crude Extract Inhibits Hepatocellular Carcinoma Growth in Mice

( Yongmin Yan ),( Yanjing Wang ),( Jiali Qian ),( Sihui Wu ),( Yi Ji ),( Yanxiao Liu ),( Jian Zeng ),( Aihua Gong ) 한국미생물생명공학회(구 한국산업미생물학회) 2019 Journal of microbiology and biotechnology Vol.29 No.8

Nattokinase (NK, E.C. 3.4.21.62) is a serine protease produced by Bacillus subtilis natto that shows promise for the treatment of thrombotic disease. In this study, we assessed the effects of NK on the development of hepatocellular carcinoma (HCC), a principal malignancy of the liver that causes morbidity and mortality worldwide. Crude extracts of NK (NCE) were isolated from fermentation medium by centrifugation and separated into three fractions (< 10 K, 100~30 K and >30K). Orthotopic HCC mouse models were established and NCE was administered by oral gavage. H&E staining was performed to examine the pathology of HCC livers. Immunohistochemistry and immunofluorescence were used to evaluate FOXM1, CD31, CD44 and vimentin expression in the liver. Compared to PBS groups, NCE increased the survival rates of HCC-bearing mice to 31% and decreased ascites. Low-intensity ultrasound imaging showed that the hypoechoic mass area was lower in NCE-treated mice and that tumor growth significantly decreased. IHC staining showed that the expression of FOXM1 was inhibited by NCE treatment. Immunofluorescence results revealed lower levels of CD31, CD44 and vimentin in the NCE groups. Taken together, these data demonstrate that NCE from Bacillus subtilis natto improves survival and inhibits tumor growth in HCC mice.

Isolation and Characterization of a Novel Bacterium Burkholderia gladioli Bsp-1 Producing Alkaline Lipase

( Jing Zhu ),( Yanjing Liu ),( Yanqin ),( Lixia Pan ),( Yi Li ),( Geliang ),( Qingyan Wang ) 한국미생물·생명공학회 2019 Journal of microbiology and biotechnology Vol.29 No.7

Active lipase-producing bacterium Burkholderia gladioli Bps-1 was rapidly isolated using a modified trypan blue and tetracycline, ampicillin plate. The electro-phoretically pure enzyme was obtained by purification using ethanol precipitation, ion-exchange chromatography, and gel filtration chromatography. The molecular weight was 34.6 kDa and the specific activity was determined to be 443.9 U/mg. The purified lipase showed the highest activity after hydrolysis with p-NPC₁₆ at a pH of 8.5 and 50°C, and the K_m, k_cat, and k_cat/K_m values were 1.05 mM, 292.95 s^-1 and 279 s^-1mM^-1, respectively. The lipase was highly stable at 7.5 ≤ pH ≤ 10.0. K⁺ and Na⁺ exerted activation effects on the lipase which had favorable tolerance to short-chain alcohols with its residual enzyme activity being 110% after being maintained in 30% ethanol for 1 h. The results demonstrated that the lipase produced by the strain B. gladioli Bps-1 has high enzyme activity and is an alkaline lipase. The lipase has promising chemical properties for a range of applications in the food-processing and detergent industries, and has particularly high potential for use in the manufacture of biodiesel.

A Three-way Handshaking Access Mechanism for Point to Multipoint In-band Full-duplex Wireless Networks

( Haiwei Zuo ),( Yanjing Sun ),( Changlin Lin ),( Song Li ),( Hongli Xu ),( Zefu Tan ),( Yanfen Wang ) 한국인터넷정보학회 2016 KSII Transactions on Internet and Information Syst Vol.10 No.7

In-band Full-duplex (IBFD) wireless communication allows improved throughput for wireless networks. The current Half-duplex (HD) medium access mechanism Request to Send/Clear to Send (RTS/CTS) has been directly applied to IBFD wireless networks. However, this is only able to support a symmetric dual link, and does not provide the full advantages of IBFD. To increase network throughput in a superior way to the HD mechanism, a novel three-way handshaking access mechanism RTS/SRTS (Second Request to Send)/CTS is proposed for point to multipoint (PMP) IBFD wireless networks, which can support both symmetric dual link and asymmetric dual link communication. In this approach, IBFD wireless communication only requires one channel access for two-way simultaneous packet transmissions. We first describe the RTS/SRTS/CTS mechanism and the symmetric/asymmetric dual link transmission procedure and then provide a theoretical analysis of network throughput and delay using a Markov model. Using simulations, we demonstrate that the RTS/SRTS/CTS access mechanism shows improved performance relative to that of the RTS/CTS HD access mechanism.

A Medium Access Control Mechanism for Distributed In-band Full-Duplex Wireless Networks

( Haiwei Zuo ),( Yanjing Sun ),( Song Li ),( Qiang Ni ),( Xiaolin Wang ),( Xiaoguang Zhang ) 한국인터넷정보학회 2017 KSII Transactions on Internet and Information Syst Vol.11 No.11

In-band full-duplex (IBFD) wireless communication supports symmetric dual transmission between two nodes and asymmetric dual transmission among three nodes, which allows improved throughput for distributed IBFD wireless networks. However, inter-node interference (INI) can affect desired packet reception in the downlink of three-node topology. The current Half-duplex (HD) medium access control (MAC) mechanism RTS/CTS is unable to establish an asymmetric dual link and consequently to suppress INI. In this paper, we propose a medium access control mechanism for use in distributed IBFD wireless networks, FD-DMAC (Full-Duplex Distributed MAC). In this approach, communication nodes only require single channel access to establish symmetric or asymmetric dual link, and we fully consider the two transmission modes of asymmetric dual link. Through FD-DMAC medium access, the neighbors of communication nodes can clearly know network transmission status, which will provide other opportunities of asymmetric IBFD dual communication and solve hidden node problem. Additionally, we leverage FD-DMAC to transmit received power information. This approach can assist communication nodes to adjust transmit powers and suppress INI. Finally, we give a theoretical analysis of network performance using a discrete-time Markov model. The numerical results show that FD-DMAC achieves a significant improvement over RTS/CTS in terms of throughput and delay.

Damage evaluation and prediction of steel box structures under internal blast

Shujian Yao,Zhifu Wang,Duo Zhang,Fangyun Lu,Nan Zhao,Yanjing Wang 대한기계학회 2022 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.36 No.10

In this study, a rapid prediction method for the damage range evaluation of steel box structures under internal blast load was proposed. First, we carried out a dimensional analysis of the damage range, determined the main influencing factors, and built a predictive model. Then, a large number of numerical simulations were conducted with consideration of different steel grades, structural dimensions, plate thicknesses, and explosive weights. After that, according to the damage range data for all the working conditions obtained with the simulation, the failure mode diagrams were drawn on a dimensionless coordinate axis and a twodimensional dimensionless number coordinate system. Based on this, the damage range for different working conditions was observed, and empirical equations were proposed to quickly predict the damage range of the steel box structure during a blast load. Finally, the correctness of the proposed quick prediction method was verified through field blast experiments result.

Outcomes of Anti-CD19 CAR-T Treatment of Pediatric B-ALL with Bone Marrow and Extramedullary Relapse

Xinyu Wan,Xiaomin Yang,Fan Yang,Tianyi Wang,Lixia Ding,Lili Song,Yan Miao,Xiang Wang,Yani Ma,Chengjuan Luo,Jingyan Tang,Longjun Gu,Jing Chen,Yanjing Tang,Jun Lu,Benshang Li 대한암학회 2022 Cancer Research and Treatment Vol.54 No.3

PurposeAnti-CD19 chimeric antigen receptor T-cell immunotherapy (19CAR-T) has achieved impressive clinical results in adult and pediatric relapsed/refractory (r/r) B-lineage acute lymphoblastic leukemia (B-ALL). However, the application and effect of CAR-T therapy in B-ALL patients with extramedullary relapse are rarely issued even disqualified in some clinical trials. Here, we examined the efficacy of 19CAR-T in patients with both bone marrow and extramedullary involvement.Materials and MethodsCAR-T cells were generated by transfection of primary human T lymphocytes with a lentiviral vector expressing anti-CD19 single chain antibody fragments (scFvs) with the cytoplasmic domains of 4-1BB and CD3ζ, and used to infuse patients diagnosed as having r/r B-ALL with extramedullary origination. Clinical responses were evaluated by the use of bone marrow aspiration, imaging, and flow cytometry. ResultsEight patients received 19CAR-T infusion and all attained complete remission (CR). Only one patient was bridged to hematopoietic stem cell transplantation (HSCT). Although three patients relapsed after infusion, they received 19/22CAR-T infusion sequentially and attained a second remission. To date, five patients are in continuous CR and all eight patients are still alive. The mean follow-up time was 21.9 months, while the 24-month estimated event-free survival is 51.4%. Conclusion19CAR-T therapy can lead to clinical remission for extramedullary relapsed pediatric B-ALL patients. However, the problem of CD19+ relapses after CAR-T remained to be solved. For patients relapsing after CAR-T, a second CAR-T therapy creates another opportunity for remission for subsequent HSCT.

Clinicopathological Analysis and Treatment of Adult Patients with Inflammatory Myofibroblastic Tumor: A 15-Year Single- Center Study

Xin Liu,Chengcheng Gong,Jieyun Zhang,Wanjing Feng,Yanjing Guo,Youzhou Sang,Chunmeng Wang,Yong Chen,Jian Wang,Lin Yu,Xiaowei Zhang,Zhiguo Luo 대한암학회 2023 Cancer Research and Treatment Vol.55 No.3

Purpose Inflammatory myofibroblastic tumor (IMT) is a rare mesenchymal malignancy that occurs primarily in children and adolescents. The clinical and pathological features of IMT in adult patients are not well understood.Materials and Methods We retrospectively searched for records of adult patients with IMT at Fudan University Shanghai Cancer Center from 2006 to 2021. Clinicopathological data, treatments, and outcomes were collected and analyzed.Results Thirty adult patients with IMT, mostly women (60.0%), were included. The median age of the patients was 38 (21-77). The most common primary site was abdominopelvic region (53.3%), followed by lungs (20.0%). Seven patients had an abdominal epithelioid inflammatory myofibroblast sarcoma (EIMS). The positivity rate of anaplastic lymphoma kinase (ALK) was 81.5% (22/27). Sixteen patients with advanced ALK-positive disease received crizotinib, with an objective response rate (ORR) of 81.3% and a disease control rate of 87.5%. The median progression-free survival was 20.8 months. EIMS was associated with more aggressive behavior; however, the prognosis was similar to that of non-EIMS patients after treatment with an ALK inhibitor. At a median follow-up time of 30 months (95% confidence interval [CI], 13.6 to 46.4), the 5-year overall survival was 77% (95% CI, 66 to 88) in all patients.Conclusion Adult IMTs appeared more aggressive, with a higher incidence of recurrence and metastases, and patients with EIMS had more aggressive cases. Treatment with ALK inhibitors resulted in a high ORR and a durable response, which suggested that ALK inhibitors could be used as a first-line treatment option in adult patients with ALK-positive advanced IMT.