Micro gadolinium oxide dispersed flexible composites developed for the shielding of thermal neutron/gamma rays

Wang Boyu,Guo Xiaolin,Yuan Lin,Fang Qinglong,Wang Xiaojuan,Qiu Tianyi,Lai Caifeng,Wang Qi,Liu Yang 한국원자력학회 2023 Nuclear Engineering and Technology Vol.55 No.5

In this study, a series of flexible neutron/gamma shielding composites are fabricated through the doping of Gd2O3 into the matrix of SEBS with (MGd2O3: MSEBS) % from 5% to 100%. Neutron transmittance test shows an exponential attenuation with the increase of areal density of Gd, in which the transmittance T ranges from 59.1440% to 35.3026%, with standard deviation less than 2.2743%, mass attenuation coefficient mm from 0.3194 cm2 /g to 0.4999 cm2 /g, and half value layer-HVL value from 2.4530 mm to 1.1313 mm. Shielding efficiency of the Gd2O3/SEBS composites is basically improved in comparison with that of B4C/SEBS. The transmittance T, mass/linear attenuation coefficient mm and m, HVL and effective atomic number Zeff for the shielding of g rays (39 keV, 59 keV and 122 keV) are measured and calculated with XCOM as well as MCX programs. Finally, plots of the three dimensional relationships between transmittance, doping amount and thickness are provided to the guidance for engineering shielding design. In summary, the Gd2O3/SEBS composite is proved to be an effective flexible neutron/low energy g rays shielding material, which could be of potential applications in the field of nuclear technology and nuclear engineering.

Considerations on Security and Trust Measurement for Virtualized Environment

Xiaolin Wang,Yan Sang,Yi Liu,Yingwei Luo 한국정보기술융합학회 2011 JoC Vol.2 No.2

Child Poverty in Rural China: Multidimensional Perspective

Xiaolin Wang,Liang Zhou,Xiaoyuan Shang 한국사회복지학회 2015 Asian Social Work and Policy Review Vol.9 No.2

This paper describes a pilot study on child multidimensional poverty in rural China by introducing the Alkire–Foster method based on survey data collected from five provinces/autonomous regions. Results showed that the headcount ratio of child multidimensional poverty was 14.29%. On average, multidimensionally poor children were deprived in 37.62% of all the dimensions, and their adjusted headcount ratio (multidimensional poverty) index was 0.054. Health was the most deprived dimension, followed by the subsistence and participation dimensions. Disabled children, children influenced by HIV/AIDS, and ethnic minority children were severely deprived. This study not only adds to the current literature on child poverty in China, but also provides policy suggestions for future action plans.

Effect of Leukocyte-Platelet Rich Fibrin (L-PRF) on Tissue Regeneration and Proliferation of Human Gingival Fibroblast Cells Cultured Using a Modified Method

Mudalal Mahmoud,Wang Zhanqi,Mustafa Shockry,Liu Yiping,Wang Yao,Yu Jize,Wang Shengnan,Sun Xiaolin,Zhou Yanmin 한국조직공학과 재생의학회 2021 조직공학과 재생의학 Vol.18 No.5

Background: An in vitro study on rapid culturing method of human gingival fibroblast cells (HGFCs) was established to investigate the potential use of the leukocyte-platelet rich fibrin (L-PRF) in tissue engineering technology, different medical fields, including periodontology and implantology. Methods: Eight biopsies were obtained from eight different donors and a modified culturing technique was developed to obtain HGFCs. The modified 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide MTT assay was used to compare the cell viability when the modified culturing method was used in comparison to the standard method. Blood samples were collected from the same patients and L-PRF was isolated using a standard protocol. The releases of platelet-derived growth factor-AA and transforming growth factor-beta1 at various time intervals were observed using enzyme-linked immunosorbent assay (ELISA) kit. The proliferative effect of L-PRF on HGFCs was assessed by the cell counting kit—8 assay. Results: A simple and rapid modified method for in vitro HGFC culture yielded a cellular monolayer within three to nine days after cell culture. L-PRF with three-dimensional polymer fibers released growth factors that peaked during the first three hours and continued to produce up to 10 days. The L-PRF presented a dose-dependent effect on HGFCs proliferation where HGFCs proliferation increased with an increase in L-PRF concentration. Conclusion: The modified technique for the culture of HGFCs might be useful for the development of future experimental and clinical studies, besides L-PRF has great therapeutic potential in oral surgery fields. Background: An in vitro study on rapid culturing method of human gingival fibroblast cells (HGFCs) was established to investigate the potential use of the leukocyte-platelet rich fibrin (L-PRF) in tissue engineering technology, different medical fields, including periodontology and implantology. Methods: Eight biopsies were obtained from eight different donors and a modified culturing technique was developed to obtain HGFCs. The modified 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide MTT assay was used to compare the cell viability when the modified culturing method was used in comparison to the standard method. Blood samples were collected from the same patients and L-PRF was isolated using a standard protocol. The releases of platelet-derived growth factor-AA and transforming growth factor-beta1 at various time intervals were observed using enzyme-linked immunosorbent assay (ELISA) kit. The proliferative effect of L-PRF on HGFCs was assessed by the cell counting kit—8 assay. Results: A simple and rapid modified method for in vitro HGFC culture yielded a cellular monolayer within three to nine days after cell culture. L-PRF with three-dimensional polymer fibers released growth factors that peaked during the first three hours and continued to produce up to 10 days. The L-PRF presented a dose-dependent effect on HGFCs proliferation where HGFCs proliferation increased with an increase in L-PRF concentration. Conclusion: The modified technique for the culture of HGFCs might be useful for the development of future experimental and clinical studies, besides L-PRF has great therapeutic potential in oral surgery fields.

Differential expression profile of microRNA in yak skeletal muscle and adipose tissue during development

Hui Ji,Hui Wang,Qiumei Ji,Winbo Ji,Xiaolin Luo,Jiabo Wang,Zhixin Chai,Jinwei Xin,Xin Cai,Zhijuan Wu,Jikun Wang,Jincheng Zhong 한국유전학회 2020 Genes & Genomics Vol.42 No.11

Background miRNAs play an important role in regulating normal animal development. Muscle tissue and fat metabolismare important for maintaining energy balance in animals. Yak has important agricultural and economic importance as itprovides milk, meat, and hair. It is used for transportation as well. However, the miRNA expression profiles of their muscleand adipose tissue are currently unknown. Objective To explore the regulatory roles of miRNAs in the skeletal muscle and adipose tissues of yak. Methods A total of 12 small RNA libraries were constructed from the skeletal muscle and adipose samples from yak aged 0.5,2.5, 4.5, and 7.5 years. High-throughput sequencing and bioinformatics analysis were used to determine the dynamic expressionprofile of miRNA, and a miRNA regulatory network related to muscle and adipose tissue development was established. Results miR-1-3p and miR-143-3p showed the highest expression during yak skeletal muscle and fat development, respectively. The MAPK and Ras signaling pathways were the pivotal pathways. miR-181-5p, miR-542-3p, and miR-424-5p mayhave key roles in skeletal muscle development, and CREBRF, GRB10, CDK1, RFX3, and EPC2 were the core target genes. While miR-127-5p, miR-379-3p, and miR-494-3p may play important regulatory roles in adipose deposition, and ETV1,XPO7, and C5AR2 were the core target genes. Conclusion This study provides valuable resources for further study of the molecular mechanisms underlying yak skeletalmuscle and adipose tissue development, and also a basis for studying the interactions between genes and miRNAs.

Comparative effect of eldecalcitol and alfacalcidol on bone microstructure: A preliminary report of secondary analysis of a prospective trial

Xiaolin Ni,Juan Feng,Yan Jiang,Li Zhang,Wei Yu,Ou Wang,Mei Li,Xiaoping Xing,Toshio Matsumoto,Weibo Xia 대한골다공증학회 2021 Osteoporosis and Sarcopenia Vol.7 No.2

Objectives: To compare the effect of eldecalcitol and alfacalcidol on skeletal microstructure by highresolution peripheral QCT (HR-pQCT). Methods: This was a substudy of a randomized, double-blind, active comparator trial. Five female osteoporotic patients with 1-year 0.75 ㎍/day eldecalcitol and 5 with 1-year 1.0 ㎍/day alfacalcidol completed HR-pQCT scans before and after treatment were enrolled. Results: Total vBMD [1.67 ± 1.06% (mean ± SD), P = 0.043 versus baseline] and trabecular vBMD (2.91 ± 1.72%, P = 0.043) at the radius increased in eldecalcitol group, while total, trabecular, and cortical vBMD tended to decrease in alfacalcidol group, with a significant reduction in cortical vBMD at the tibia (0.88 ± 0.62%, P = 0.043). Cortical area (1.82 ± 1.92%, P = 0.043) at the radius and thickness (0.87 ± 1.12%, P = 0.043) at the tibia increased in eldecalcitol group, while these parameters decreased with alfacalcidol at the tibia (1.77 ± 1.72%, P ¼ 0.043 for cortical area; 1.40 ± 2.14%, P = 0.042 for cortical thickness). Trabecular thickness at the radius (1.97 ± 1.93%, P = 0.042) and number at the tibia (3.09 ± 3.04%, P = 0.043) increased by eldecalcitol but did not increase by alfacalcidol. Trabecular separation decreased by eldecalcitol (2.22 ± 2.43%, P = 0.043) but tended to increase by alfacalcidol at the tibia. Conclusions: Eldecalcitol has the greater potential to improve cortical and trabecular microstructure at the peripheral bone than alfacalcidol which needs further more studies.

Electric-Field-Assisted Assembly of Polymer-Tethered Gold Nanorods in Cylindrical Nanopores

Wang, Ke,Jin, Seon-Mi,Xu, Jiangping,Liang, Ruijing,Shezad, Khurram,Xue, Zhigang,Xie, Xiaolin,Lee, Eunji,Zhu, Jintao American Chemical Society 2016 ACS NANO Vol.10 No.5

<P>In this report, we demonstrate the confined assembly of polymer-tethered gold nanorods in anodic aluminum oxide (AAO) channels with the assistance of electric field (EF). Various interesting hybrid assemblies, such as single-, double-, triple-, or quadruple-helix, linear, and hexagonally packed structures are obtained by adjusting pore size in AAO channels, ligand length, and EF orientation. Correspondingly, surface plasmonic property of the assemblies can thus be tuned. This strategy, by coupling of external-field and cylindrically confined assembly, is believed to be a promising approach for generating ordered hybrid assemblies with hierarchical structures, which may find potential applications in photoelectric devices, biosensors, and data storage devices.</P>

Non-interfacial self-assembly of synthetic protocells

Xiaolin Xu,Wencai Guan,Xiaolei Yu,Guoxiong Xu,Chenglong Wang 한국생체재료학회 2023 생체재료학회지 Vol.27 No.00

Background Protocell refers to the basic unit of life and synthetic molecular assembly with cell structure and function. The protocells have great applications in the field of biomedical technology. Simulating the morphology and function of cells is the key to the preparation of protocells. However, some organic solvents used in the preparation process of protocells would damage the function of the bioactive substance. Perfluorocarbon, which has no toxic effect on bioactive substances, is an ideal solvent for protocell preparation. However, perfluorocarbon cannot be emulsified with water because of its inertia. Methods Spheroids can be formed in nature even without emulsification, since liquid can reshape the morphology of the solid phase through the scouring action, even if there is no stable interface between the two phases. Inspired by the formation of natural spheroids such as pebbles, we developed non-interfacial self-assembly (NISA) of microdroplets as a step toward synthetic protocells, in which the inert perfluorocarbon was utilized to reshape the hydrogel through the scouring action. Results The synthetic protocells were successfully obtained by using NISA-based protocell techniques, with the morphology very similar to native cells. Then we simulated the cell transcription process in the synthetic protocell and used the protocell as an mRNA carrier to transfect 293T cells. The results showed that protocells delivered mRNAs, and successfully expressed proteins in 293T cells. Further, we used the NISA method to fabricate an artificial cell by extracting and reassembling the membrane, proteins, and genomes of ovarian cancer cells. The results showed that the recombination of tumor cells was successfully achieved with similar morphology as tumor cells. In addition, the synthetic protocell prepared by the NISA method was used to reverse cancer chemoresistance by restoring cellular calcium homeostasis, which verified the application value of the synthetic protocell as a drug carrier. Conclusion This synthetic protocell fabricated by the NISA method simulates the occurrence and development process of primitive life, which has great potential application value in mRNA vaccine, cancer immunotherapy, and drug delivery.

Transcription factor Sp1 is necessary and functional in regulating expression of oncogene ZNF703

Xiaolin Liao,Yongjie Lu,Junbao Yang,Tao Kuang,Lilin Jiang,Yanjun Wang,Huiqun Kang,Bo Jiang,Xiaoli Zhou,Sheng He 한국유전학회 2017 Genes & Genomics Vol.39 No.10

Zinc finger protein 703 (ZNF703) is a putative oncogene in patients with the luminal B molecular subtype of breast cancer. Although the exact function of ZNF703 protein remains largely unknown, its expression and regulation have been implicated in several physiological and pathological processes. In the current study, for the first time, we identified and characterized the human ZNF703 gene promoter region. As a means of characterizing the transcription elements required for expression of ZNF703 protein at different stages, we cloned the promoter region of ZNF703 then created chimeric reporter plasmids for use in luciferase assays. A progressive deletion analysis of the ZNF703 gene’s 5′ and 3′ -flanking regions revealed that the core promoter is located in a 256-bp region ranging from nt-539 to nt-283. Next, we examined the effects of sitespecific mutations and treatment with mithramycin A to identify the functional Sp1 binding site, which was found to be located in a 447 bp region that ranged from nt-509 to nt-76, displayed the characteristics of a CpG island, and overlapped with the promoter region. In conclusion, our data suggest that ZNF703 transcription is regulated by transcription factor Sp1. This finding should facilitate future studies of the mechanism which regulates expression of this important gene.

Parameters Estimation of Hinging Hyperplanes using Median Squared Error Criterion

Xiaolin Huang,Jun Xu,Shuning Wang 제어·로봇·시스템학회 2011 International Journal of Control, Automation, and Vol.9 No.4

This paper considers parameter estimation for nonlinear model using median squared error (MSE) criterion, which is limited to linear model in the past. It is shown that applying MSE, the essence of estimating parameters for hinging hyperplanes (HH) and linear model are the same. Motivated by this fact, MSE estimation is discussed for HH. A local optimality condition is given and based on this condition, an algorithm using linear programming technique is proposed. Numerical experiments show the good performance of the proposed estimation strategy and algorithm.