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( Jing Zhu ),( Yanjing Liu ),( Yanqin ),( Lixia Pan ),( Yi Li ),( Geliang ),( Qingyan Wang ) 한국미생물·생명공학회 2019 Journal of microbiology and biotechnology Vol.29 No.7
Active lipase-producing bacterium Burkholderia gladioli Bps-1 was rapidly isolated using a modified trypan blue and tetracycline, ampicillin plate. The electro-phoretically pure enzyme was obtained by purification using ethanol precipitation, ion-exchange chromatography, and gel filtration chromatography. The molecular weight was 34.6 kDa and the specific activity was determined to be 443.9 U/mg. The purified lipase showed the highest activity after hydrolysis with p-NPC<sub>16</sub> at a pH of 8.5 and 50°C, and the K<sub>m</sub>, k<sub>cat</sub>, and k<sub>cat</sub>/K<sub>m</sub> values were 1.05 mM, 292.95 s<sup>-1</sup> and 279 s<sup>-1</sup>mM<sup>-1</sup>, respectively. The lipase was highly stable at 7.5 ≤ pH ≤ 10.0. K<sup>+</sup> and Na<sup>+</sup> exerted activation effects on the lipase which had favorable tolerance to short-chain alcohols with its residual enzyme activity being 110% after being maintained in 30% ethanol for 1 h. The results demonstrated that the lipase produced by the strain B. gladioli Bps-1 has high enzyme activity and is an alkaline lipase. The lipase has promising chemical properties for a range of applications in the food-processing and detergent industries, and has particularly high potential for use in the manufacture of biodiesel.
Bing Xia,Jian Shang,Xiaobing Wang,Wei Wang,Huaqin Pan,Shi Liu,Lixia Li,Liping Chen 연세대학교의과대학 2016 Yonsei medical journal Vol.57 No.1
Purpose: The -1237T/C polymorphism of the Toll-like receptor 9 (TLR9) gene has been implicated in the susceptibility of inflammatorybowel diseases (IBDs), but the results remain conflicting. We further investigated this association via meta-analysis. Materials and Methods: Multiple electronic databases were extensively searched until February, 2015. The strength of associationwas evaluated by calculating the pooled odds ratios (ORs) and 95% confidence intervals (CIs). Results: A total of 2987 cases and 2388 controls from eight studies were analyzed. Overall, association was found between TLR9 -1237T/C polymorphism and the risk of IBDs when all the studies were pooled (recessive model, OR: 1.59, 95% CI: 1.02–2.47, p=0.04; homozygote comparison, OR: 1.62, 95% CI: 1.04–2.52, p=0.03; allele model, OR: 1.13, 95% CI: 1.00–1.27, p=0.05). Stratificationby ethnicity indicated an association between TLR9 -1237T/C polymorphism and IBDs risk in Caucasians (recessive model,OR: 1.59, 95% CI: 1.02–2.47, p=0.04; homozygote comparison, OR: 1.62, 95% CI: 1.04–2.52, p=0.03; allele model, OR: 1.12, 95% CI: 1.00–1.27, p=0.05). When stratified by disease type, significant correlation were only found in the Crohn’s disease subgroup (recessive model, OR: 1.69, 95% CI: 1.05–2.73, p=0.03; homozygote model, OR: 1.74, 95% CI: 1.07–2.82, p=0.02; allele model, OR: 1.15, 95% CI: 1.01–1.32, p=0.04). Conclusion: The present study suggested that the TLR9 -1237T/C polymorphism might act as a risk factor in the development of IBDs, particularly in Caucasians.