Slide Session : OS-IFD-07 ; Infectious Disease : In Vitro Antiviral Activity of Ribavirin Against Severe Fever with Thrombocytopenia Syndrome Virus

( Myung Jin Lee ),( Kye Hyung Kim ),( Jong Youn Yi ),( Su Jin Choi ),( Chung Jong Kim ),( Nak Hyun Kim ),( Kyoung Ho Song ),( Pyoeng Gyun Choi ),( Ji Hwan Bang ),( Wan Beom Park ),( Eu Suk Kim ),( San 대한내과학회 2014 대한내과학회 추계학술대회 Vol.2014 No.1

In Vitro Antiviral Activity of Ribavirin Against Severe Fever with Thrombocytopenia Syndrome Virus Myung Jin LEE1, Kye-Hyung KIM1, Jongyoun YI2, SuJin CHOI1, Chung-Jong KIM1, Nak- Hyun KIM1, Kyoung-Ho SONG1, Pyoeng Gyun CHOI1, Ji-Hwan BANG1, Wan Beom PARK1, Eu Suk KIM1, Sang-Won PARK1, Hong Bin KIM1, Nam Joong KIM1, Myoung- Don OH1 Seoul National University College of Medicine, Korea1, Pusan National University School of Medicine, Korea2 Background: Severe fever with thrombocytopenia syndrome (SFTS) is an emerging infectious disease caused by a novel Bunyavirus, severe fever with thrombocytopenia syndrome virus (SFTSV). No effective antiviral therapy is proven yet, but clinical use of ribavirin (RBV) has been tried. We investigated the antiviral effect of RBV against SFTSV in vitro. Methods: To test for cytotoxicity of RBV, Vero cells were treated with different concentrations of RBV (3.90 to 500 μg/mL, two-fold dilution) and analyzed by cell viability MTS assay 48h post-infection. To determine antiviral activity of RBV against SFTSV, Vero cells were infected with SFTSV strain Gangwon/Korea/2012 at 100 TCID50 (50% tissue culture infective dose) per well in a 96-well plate, and RBV was added at the concentrations showing no or minimal cytotoxicity. Viral RNAs were extracted from the culture supernatants and quantifi ed using one-step real-time reverse transcription- PCR to amplify the partial large segment of SFTSV. Statistical analysis was done by one-way ANOVA with Tukey`s post hoc test. Results: Cytotoxicity due to RBV was not observed at RBV concentration =31.3 μg/ mL. Viral RNAs at 24h post-RBV treatment were reduced with increasing RBV concentrations (1-32 μg/mL), compared with those of mock-treated cells (P <0.01, Figure). Half maximal inhibitory concentration (IC50) of RBV was 3.69 μg/mL at 24h post-RBV treatment. Conclusions: Our study shows that RBV has antiviral effect against SFTSV in a dose-dependent manner. Further studies are required to evaluate the effi cacy of RBV in SFTS.

디지털 카메라의 자체검정을위한 도심측정방법과 타원 피팅 방법의 정확도 분석

박운용,정공운,송연경 東亞大學校 建設技術硏究所 2002 硏究論文集 Vol.26 No.1

Digital high-resolution consumer cameras are widely available, and are increasingly used in close-range photogrammetry. The mechanical construction of the cameras often do not meet photogrammetric requirements, thus they have to be modelled sufficiently. An approach for camera modelling is discussed that introduces image-variant parameters of interior orientation. In addition a correction model based on finite elements is integrated that provides the correction of remaining errors in the sensor space. All functional parameters are estimated simultaneously in a bundle adjustment. Significant improvements of object accuracy have been achieved with respect to standard calibration techniques based on self-calibrating bundle adjustment. Rollei d7 metric 디지털 카메라를 이용하여 촬영조건의 다양한 변화에 따른 위치오차를 분석하며 대상물표면에 설치된 타켓의 3차원위치를 결정하기 위해 도심측정방법과 타원 피팅 방법으로 타켓중심을 구하였다. 각 타켓의 변위를 측정하고 3차원 정확도를 분석한 결과 다음과 같은 결론을 얻었다. 도심측정방법과 타원 피팅 방법을 여러 가지로 조합하여 적용한 결과 평균표준오차는 타원 피팅 방법과 도심측정방법을 동시에 적용되었을 경우 X좌표에서 ±0.478mm, Y좌표에서 ±0.478mm, Z좌표에서 ±0.478 mm로 가장 낮은 오차를 보였다. 도심측정방법은 타켓의 모양에 구애받지 않으나 적절한 임계값의 결정이 중요하며 경중률 을 고려함으로써 이를 보완할 수 있었다. 반면에 타원 피팅 방법은 타켓의 형상이 수학적으로 피팅 할 수 있는 형상이어야 한다는 점은 불편하나 임계값 영향을 받지 않았다. 자체 검정기법에 의한 촬영조건의 다양한 변화에 따른 위치오차를 분석하여 Rollei d7 metric 디지털 카메라의 기하학적 지식 및 수행성을 평가하기 위하여 내부표정요소 및 렌즈 왜곡 등에 관련된 기하학적 검정 매개변수들을 도출해 낼 수 있었으며 높은 정확 도 를 나타냄으로써 근거리 지상사진측량시스템 의 잠재성을 확인할 수 있었다. 자체검정 광속조정은 카메라의 계통적 오차들을 효과적으로 보정해줌으로써 정확도를 크게 향상시킬 수 있었다. 따라서 자체검정 기법을 적용하는 디지럴 카메라에 의한 광학적 근거리 지상사진측량시스템은 산업의 차원적 품질 검사,문화재의 3차원 모델링 및 형상재현 등을 효율적으로 구현하여 대상물의 시각적 분석에 활용할 수 있을 것이다.

Actazolamide와 Fluphenazine의 뇌척수액 생성에 대한 효과

송준혁,박윤관,정흥섭,서중근,이훈갑,이기찬,주정화 대한신경외과학회 1993 Journal of Korean neurosurgical society Vol.22 No.10

The purpose of this study was to investigate the effect of acetazolamide and fluphenazine on the formation of CSF. Studies were performed in 12 cats those were divided into 2 groups ; A-F group included animals received initial acetazolamide infusion and additional infusion of fluphenazine to the initial infusion and the F-A group for vice versa. The rate of CSF formation was measured at 3㎝ above zero outflow pressure by force transducer which connected to personal computer. After obtaining steady value of CSF formation rate, the drugs were infused intravenously according to the protocol. Base line CSF formation rate, 18.87±6.52μl/min, is reduced to 6.67±2.45μl/min after acetazolamide infusion and further reduced to 3.48±4.06μl/min after additional fluphenaxine. In fluphenazine group, the base line CSF formation rate, 16.34±4.58μl/min is reduced to 9.63±4.58μl/min after initial infusion of fluphenazine and further to 6.45±3.64μl/min, after additional infusion of acetazolamide. Mean reduction of CSF formation after initial intravenous infusion of acetazolamide and fluphenazine were 59% and 37% respectively. Although statistically insignificant, the CSF formation reduction in A-F group revealed more even and profound value comparing with that of F-A group. These date suggest that in addition to the effect of acetazolamide to reduce the formation of CSF, some other mechanism may exist in CSF formation that major tranquilizer exert the effect on CSF formation.

Nimodipine이 뇌척수액생성에 미치는 효과에 관한 실험적 연구

송준혁,박윤관,정흥섭,서중근,이훈갑,이기찬,주정화 대한신경외과학회 1993 Journal of Korean neurosurgical society Vol.22 No.9

The author has performed an experimental study in order to investigate the effect of nimodipine on the production of CSF in 12 cats. The cats were divided into 2 groups, one for nimodipine intravenous infusion and the other for its vehicle infusion group. Using ventriculo-cisternal perfusion method nimodipine and its vehicle were examined for their effects on CSF formation rate respectively. Baseline CSF formation rate was 22.5±2.9㎕/min(S.E.) and it gradually reduced to 17.0±3.4㎕/min(S.E.) after final infusion of nimodipine at 60㎍/㎏/min. Vehicle infusion revealed no significant change in CSF formation rate. Although the nimodipine insuion revealed declining tendency in CSF formation rate along with increment on nimodipine concentration, it was not statistically different from that of vehicle infusion group. Systolic blood pressure was significantly reduced after nimodipine infusion(133±31.8mmHg at baseline, 93.9±19.1mmHg at the end of the experiment) on oneway ANOVA test and it was significantly different from that in vehicle infusion group(p=<0.01).

왜소음경증과 남성 성기능에 관한 연구

송윤섭,김민의,이남규,박영호,전윤수,김응구 순천향의학연구소 1997 Journal of Soonchunhyang Medical Science Vol.3 No.2

Micropenis is defined as a normally formed penis with a size greater than 2 standard deviations below the mean. Twenty three patients with micropenis were managed at Soonchunhyang University Hospital from January 1988 to December 1995 We interviewed and examined them with micropenis to investigate the cause of micropenis, concomitant diseases, the efficacy of treatment and their sexual activity in postpubertal men. The patients were divided into 2 groups: 16 prepubertal and 7 postpubertal men. 9 patients had cryptorchidism, 3 of them were bilateral and 5 patients had hypospadias. Etiology was determined by hormonal assay including HCG stimulation test ; 8 as hypogonadotrophic hypogonadism, 4 as primary testicular dysfunction and 5 as idiopathic. However etiology was undetermined in 6 patients. All patients had endocrine therapy using HCG or depot testosterone. All except 4 cases responded satisfactorily. All postpubertal patients(group 2) were below the 10th percentile. Puberty was delayed in 3 patients in the older group. All patients were heterosexual and they have had erections and orgasms. Five patients have had ejaculations, 3 of them were sexually active and they have experienced vaginal penetration, Nevertheless, their sexual partners have had complaints and the patients themselves have felt ashamed and dissatisfied. Fortunately, they were mentally healthy. None of these patients were married or had a child.

AHRS을 이용한 자세결정과 Heading 산출을 위한 연구

박운용,백기석,송연경 東亞大學校 大學院 2004 大學院論文集 Vol.29 No.-

In this paper, the error compensation method of the low-cost IMU is proposed. In general, the position and attitude error calculated by accelerometers and gyros grows with time. Therefore the additional information is required to compensate the drift. The attitude angles can be bound accelerometer mixing algorithm and the heading angle can be aided by single antenna GPS velocity. The Kalman filter is used for error compensation. The result is verified by comparing with the attitude calculated by Attitude Heading Reference System with Micro Electro Mechanical System for a basis

Accuracy on the Edge Detection of Topography for Digital Map Manufacture

Park, Woon-Yong,Song, Youn-Kyung,Bae, Kyung-Ho,Kim, Yong-Suk 東亞大學校 建設技術硏究所 2005 硏究論文集 Vol.28 No.2

Currently, digital maps in the metropolitan area are supposed to be revised and renewed every two years, but it is difficulty for the renewal of current information to keep pace with the quickly changing society and so, one of the alternative methods being discussed is a digital map produced using high resolution satellites. The biggest problem in the production of digital maps using high resolution satellite images is the difficulty of production in vector resource format through edge detection of satellite images, a Raster data. In this study, topographical boundaries were detected through edge detection of the satellite image, and after vectorliezing using the screen digitizing method, the results were evaluated and compared to the digital map from the NGII(National Geographic Information Institute) for accuracy.

지하구내에 설치된 변압기의 극간 거리에 따른 연면방전 특성

박하용,임윤희,심낙순,이상교,송우창 三陟大學校 産業科學技術硏究院 2005 産業科學技術硏究論文集 Vol.10 No.-

The electric equipment of the recently building is established in underground of the building, it is the actual condition where the immediacy countermeasure preparation which is special regarding an electric equipment is deficient. The dissertation which it sees the case where the mold transformer which is established in the underground enclosure will be flooded minimizes the damage regarding the 1st disaster it could occur and a 2nd disaster in the test Book of Psalms to select the mold transformer which at fundamental phase of the research for is established on th actual underground enclosure, it analyzed the surface discharge voltage which it follows in distance and the electric current corrugated back. It appears with the fact that the recording surface discharge voltage where the analysis result electrode for interval will become larger increases, peek of discharge current highly there is a possibility of knowing the burden. Also, it caused by with increase of the surface discharge hour and the recording discharge hour when the interval of electrode for will become larger to increase also the insulation scandal damage will be augmented with the fact that it is thought.

여성 하부요관석환자에서 무마취하 요관경하 배석술

박영호,송윤섭,임용순,김영호,이남규,구자현,서병욱,김민의,전윤수 순천향의학연구소 1999 Journal of Soonchunhyang Medical Science Vol.5 No.2

Although ureteroscopy(URS) has been used widely to remove ureteral calculi, there is an argument against the belief that anesthesia or admission is needed for URS. We evaluated the experience of URS without anesthesia in female patients. From September 1997 to July 1998, URS was performed in 31 female patients without anesthesia. EHL(Electrohydraulic Lithotripsy) was used in 21 patients with impacted or large stone over 5mm. All patients were given oral antibiotics for 3 days and follow-up KUB was taken to confirm successful removal of the stone one week after the procedure. Successful treatment was defined as complete removal of the stone or residual fragments less than 2mm. Overall success rate was 96.7%(30 cases among 31 cases). All but one patient tolerated with analgesia through the entire procedures. There were only minimal complications such as mild flank pain and hematuria. So, we recommend that non-anesthetic ureteroscopic removal of stone as the first choice for treatment of lower ureteral stones in female based on the minimal morbidity, high success rate and low cost.

토끼에서 Etofenprox와 Spinosad 합제의 국소자극성 시험

황윤환,김명석,송인배,박병권,양승호,임용현,송치용,안성한,남현수,임정교,정상일,명노일,윤효인 忠南大學校 獸醫科大學 附設 動物醫科學硏究所 2008 動物醫科學硏究誌 Vol.15 No.1

This study was performed to evaluation the skin and eye irritation of etofenprox 8% and spinosad 4% combination in male New Zealand White rabbits. In skin irritation test, the combination of etofenprox and spinosad did not showed any change of body weight but caused slight skin irritation with the P.I.I. (primary irritation index) value 0.25. The etofenprox and spinosad combination showed severe eye irritation 1 h, 24 h, 48 h, and 72 h after ocular treatment, having the A.O.I. (acute ocular irritation) value of 37.3. Therefore, these result indicated that the combination of etofenprox and spinosad might be irritant formulation on eye and skin.