심근 허혈-재관류 백서 모델에서 mitogen-activated protein kinase 활성화에 대한 α-lipoic acid의 효과

김연경,박은미,최준호,윤경호,유남진,이은미,김남호,정진원,김민선,박병림,오석규 圓光大學校 醫科學硏究所 2006 圓光醫科學 Vol.21 No.1

치과용 접착제가 복합레진 인레이와 레진시멘트의 결합력에 미치는 영향

송미혜,박수정,조현구,황윤찬,오원만,황인남 大韓齒科保存學會 2008 Restorative Dentistry & Endodontics Vol.33 No.5

본 연구는 레진시멘트의 레진인레이에 대한 접착 시 접착제 혹은 primer의 사용이 결합력에 영향을 미치는지 평가하기위해 시행하였다. 직경 5 mm,높이 4.5 mm의 원기둥 형태로 제작한 레진인레이(Tescera, Bisco, USA)의 표면을 1000번,1500번 그리고 2000번 사포로 주수 하에서 연마하여 평편한 면을 형성한 후 레진인레이의 표면에 sandblasting을 시행한 후 표면에 1분 동안 silane을 도포하였다. 2군으로 나누어 한 군은 대조군으로,다른 한 군은 표면에 동일한 제조사의 접착제나 프라이머를 도포한 후 중합하였다. 레진인레이 상에 3 mm 직경의 구멍이 형성된 아크릴판을 고정하고,구멍에 레진 시멘트를 주입하여 경화시켰다. 레진 시멘트는 Panavia-F(Kyrary), Varolink-II(Ivoclar-Vivadent), RelyX Unicem (3M ESPE),Duolink (Bisco)와 자가중합형인 Multilink(Ivoclar-Vivadent)를 사용하였다. 제작된 시편을 만능물성시험기에 위치시고 전단결합강도를 측정하였다. 측정 결과 접착제나 프라이머를 도포한 군은 대조군에 비해 전단결합강도가 증가하였다 (p < 0.05). 하지만 Variolink-II와 Panavia-F는 전단결합강도의 차이를 보이지 않았다. 대조군에서는 각 레진 시멘트 간에 전단결합강도의 유의한 차이를 보였으며 (p < O.O1),Variolink-II가 가장 높은 결합강도를 보인 반면,자가중합형인 Multilink가 가장 낮은 결합강도를 보였다. 하지만,접착제나 프라이머를 도포한 군에서는 각 제품 간에 전단결합강도의 차이를 보이지 않았다. 이상의 결과 이원중합형이나 자가중합형 레진시멘을 이용하여 레진인레이 부착 시 silane 처리 후 접착제나 프라이머의 도포가 결합 강도의 증가를 위해 필요하리라 사료된다. This study analyzed the influence of dental adhesive/primer on the bond strength between indirect resin composite and the resin cement. Seventy disc specimens of indirect resin composite (Tescera Dentin. Bisco) were fabricated. And bonding area of all specimens were sandblasted and silane treated for one minute. The resin cements were used with or without application of adhesive/primer to bonding area of indirect resin restoration: Variolink-II (Ivoclar-Vivadont): Exite DSC, Panavia-F (Kuraray): ED-Primer, RelyX Unicem (3M ESPE): Single-Bond. Duolink (Bisco): One-step. Mulitlink (Ivoclar-Vivadent): Multilinh Primer. Shear bond strength was measured by Instron universal testing machine. Adhesive application improved shear bond strength (p < 0.05). But Variolink II and Panavia-F showed no statistically significant difference according to the adhesive application. With the above results. when resin inlay is luted by resin cement it seems that application of dental adhesive/primer is necessary in order to improve the bond strength.

간호전문대학 재학생과 졸업생의 학사학위 취득 프로그램에 대한 요구도 조사

박송자,제미순 한국전문대학교육연구학회 2003 한국전문대학교육연구학회논문집 Vol.4 No.1

All of the education system is pointing toward the open education, the continuing education and the life long education all over the world. Among our nursing education institutes is being discussed the intensive major course and the credit bank system for nursing college graduates as central themes, in addition to the RN-BSN(Registered Nurse-Bachelor of Science in Nursing) being operated at present. The purpose of this study is to investigate the needs on bachelor's degree program of nursing students and graduates in junior college. This research was designed as a descriptive study by method of questionnaire. Data were collected for three months from September 10 to November 30, 2002. Subjects were 504 nursing college students and 215 nurses in working at five hospitals and four public health centers in the capital and its suburbs and country. The collected data were analyzed using SPSS 8.0 program. According to the results of study, the needs on BSN of students(60.l%) and graduates(93.0%) were high. But only 19.1% of graduates received bachelor's degree. The degree of participation in the continuing education such as in service training program was 90.1%. It happened that most of students and graduates wanted to continue their education through the intensive major course. Especially graduates to choose continuing education demanded to include contents that they can supplement to the insufficient and use at their job.

중환자실 병원감염에 대한 다기관 공동 조사연구

박은숙,박미라,진혜영,김정은,최정실,송영구,최영화,이위교,홍성관,허애정,양동규,김준명 대한병원감염관리학회 2003 의료관련감염관리 Vol.8 No.1

접근-회피 성취 목표의 타당도 연구

박영석,성미송 가톨릭대학교 사회과학연구소 2003 社會科學硏究 Vol.19 No.-

본 연구는 개인의 성취 목표 동기가 수행-접근, 수행-회피, 숙달-접근, 그리고 숙달-회피의 네 가지로 구성되어 있다는 2 × 2 성취 목표 모델(Elliot & McGregor, 2001)의 타당도를 검증하기 위해 수행되었다. 국내 기업의 임원 47명과 부장 234명에게 성취 목표를 측정하였고, 그들의 상사 1명, 동료 2명, 그리고 부하 10명이 그들의 리더십을 평가하였다. 공변량구조분석 결과 2 × 2 성취 목표 모델의 구조가 재검증되었고, 이 네 가지 성취 목표의 수렴및 변별 타당도가 함께 측정한 가치, 문제해결 방식, 리더 행동의 상관관계 분석을 통해 확인되었다. 끝으로 2 × 2 성취 목표 모델이 국내에 일반화될 수 있고, 특히 리더의 행동을 예측하는데 유용하게 활용될 수 있다는 점을 논의하였다.

두개골 및 두개봉합부 초기발육과정에서의 전사조절인자인 Msx2와 DIx5의 역할

송민호,박미현,남순현,김영진,류현모,김현정 大韓小兒齒科學會 2003 大韓小兒齒科學會誌 Vol.30 No.3

두개봉합부의 조기융합으로 일컬어지는 craniosynostosis는 두개봉합부에서의 골아세포의 조기분화 및 석회화의 결과로 나타나는 선천성 발육이상이다. 최근 유전학적 연구에 의하면 homeobox gene인 Msx2의 변이에 의해 Boston-type craniosynostosis가 야기되며, 또한 Dlx5 homozygote mutant mouse의 표현형에서 두개골의 골화지연을 포함한 다양한 두개안면부위의 이상을 발견하였다는 보고가 있었다. 게다가 Msx2와 Dlx5 homeodomain protein의 상호작용에 의해 성숙골아세포의 표지자인 osteocalcin의 전사를 조절할 수 있다는 사실이 알려져 있다. 이러한 일련의 결과들은 Msx2, Dlx5 및 osteocalcin 유전자들이 두개골의 골화과정과 두개봉합부의 형태발생에 중요한 역할을 담당하고 있음을 제시해주고 있다. 두개골의 성장과 두개봉합부의 형태발생시 이러한 유전자들의 기능을 알아보기위해 mouse의 태생기 (E15-E18) 동안 osteocalcin, Msx2, 및 Dlx5 유전자들의 발현양상을 조사하였다. Osteocalcin은 E15부터 두정골의 골막에서 관찰되었으며, 발생시기가 후기일수록 강한 발현양상을 나타내었다. Msx2는 시상봉합부의 미분화간엽조직과 osteogenic front에서 강하게 발현되었으며 경막과 hair follicle에서도 관찰되었다. Dlx5는 osteogenic front를 포함한 두정골의 골막에서 강하게 발현되었으나 시상봉합부의 미분화간엽조직에서는 발현되지않아, Msx2와는 발현양상의 차이를 나타내었다. 이 결과들을 종합해볼 때, Msx2와 Dlx5 유전자는 두개골과 두개봉합부의 성장발육과정에 중요한 역할을 담당하고 있으며, BMP signaling은 두 전사조절인자들을 조절하므로써 두개골의 골화과정과 두개봉합부의 형태발생 및 유지에 관여하고 있음을 제시해주고 있다. 특히 BMP signaling에 specific downstream gene인 Msx2 및 Dlx5의 발현양상의 차이는 골아세포의 분화시 이들 유전자가 각각의 독특한 기능을 가지고 있음을 시사해주고 있다. Craniosynostosis, known as a premature fusion of cranial sutures, is a developmental disorder characterized by precocious differentiation and mineralization of osteoblasts in the calvarial sutures. Recent genetic studies have demonstrated that mutation in the homeobox gene Msx2 causes Boston-type human craniosynostosis. Additionally, the phenotype of Dlx5 homozygote mutant mouse presents craniofacial abnormalities including a delayed ossification of calvarial bone. Furthemore transcription of osteocalcin, a mature osteoblast marker, is reciprocally regulated by the nomeodomain proteins Msx2 and Dlx5. These facts suggest important roles of osteocalcin, Msx2 and Dlx5 genes in the calvarial bone growth and suture morphogenesis. To elucidate the function of these molecules in the early morphogenesis of mouse cranial sutures, wer have first analyzed by in situ hybridization the expression of osteocalcin, Msx2 and Dlx5 genes in the developing parietal bone and sagittal suture of mouse calvaria during the embryonic (E15-E18) stage. Osteocalcin mRNA was found in the periosteum of parietal bones from E15, and gradually more highly expressed with aging. Msx2 mRNA was intensely expressed in the sutural mesenchyme, osteogenic fronts and midly expressed in the dura mater during the embryonic stage. Dlx5 mRNA was intensely expressed osteogenic fronts and the periostem of parietal bones. To further examine the upstream signaling molecules of transcription factor Msx2 and Dlx5, we have done in vitro experiments in E15.5 mouse calvarial explants. Interestingly, implantation of BMP2-, BMP4-soaked beads onto the osteogenic fronts after 48 hours organ culture induced etopic expressions of Msx2 and Dlx5 genes. On the other hand, overexpression of TGFβ1, GDF-6, -7, FGF-2, -4 and Shh did not induce the expression of Msx2 and Dlx5. Taken together, these data indicate that transcription factor Msx2 and Dlx5 play critical roles in the calvarial bone and suture development, and that BMP siganling is involved in the osteogenesis of calvarial bones and the maintenance of cranial sutures through these two transcriotpn factors. Furthermore, different expression patterns between Msx2 and Dlx5 suggest their specific functions in the osteoblast differentiation.

산지별 추정벼의 형태적 특성 및 물리적 특성 비교

박미현,우상규 漢陽大學校 韓國生活科學硏究所 1993 韓國 生活 科學 硏究 Vol.- No.11

A comparative study for physical properties of chucheongbyeo grown in Icheon and other area such as Suwon, Iri and Milyang, was made by T-test. The results are as follows. 1.In weight, shape and length of rice grains as paddy, brown rice and milled rice, Icheon rice was significantly(p<0.05) longer other rices. 2.Physical properties : No significant difference was observed in milling, whiteness and color differences. No significant difference was observed in Alkali digestibilitiy and Anylogram. In Gel consistency, Icheon rice tended to be higher in gel flow than Suwon and Milyang rice but lower than Iri rice.

수학학습의 측정 영역에 대한 초등학생의 학업 성취도 분석

송미정,박종서 진주교육대학교 초등교육연구소 2005 科學敎育硏究 Vol.28 No.-

The purpose of this study is to investigate the degree of mathematics learning achievements of elementary students in measurement area. In order to achieve these purposes, the following research problems were set up for the present study: First, we analyzes some differences on mathematics learning achievements in measurement area by schools. Second, we analyzes the degree of mathematics learning achievement in the measurement area according to the contents of learning objectives by each grade. The results of this study are as follows: In conclusion, in mathematics learning achievements, it is thought that there is a difference between Jinju city and small town(eup) and township(myeon) area and that the higher grade the students have showed the lower students' mathematics learning achievements in measurement area. So, teacher must try to cover it in the school field and to concern it much more. Also, mathematics learning just related to reality must be enforced to make students have a conceptual understanding through various experiences in actual life and the activities operating concrete objects.

도축돈 장분변으로부터 Shiga Toxin-Producing Escherichia coli의 분리와 성상

송영환,김지영,채미경,박창식,김명철,전무형 충남대학교 형질전환복제돼지연구센터 2007 논문집 Vol. No.10

Shiga toxin-producing Escherichia coli (STEC) causes various clinical signs in human and animals, and has been indicated as a global enteropathogen with zoonotic importance. In this study, the feces of healthy pigs were collected from the slaughtered pigs of Daejon abattoir during the period from December 2001 to October 2002. Of 326 specimens, 13 STEC were confirmed by culture, PCR and colony hybridization. The isolates were further studied for toxin types, pathogenic factors, plasmid profiles, and antimicrobial resistance to characterize the genetic and toxigenic properties. In PCR, all of 13 isolates were evident to have shiga toxin gene (stx). Of 13 isolates stx1 gene was detected in 4 and stx2 gene in 9. The genes of eaeA, hlyA and rfbE were not present in any isolates. In colony hybridization using shiga toxin common primer (STXc), 2 to 9 per 100 colonies subcultured from 13 isolates showed the positive reaction. In the examination for plasmid profiles of the isolates, one to eleven plasmids with varying sizes of 1.0 Kb to 100 Kb were detected, and the 13 STEC could be classified into four groups by the plasmid patterns. The antimicrobial resistance patterns of the isolates were comparably corresponded with the plasmid profile patterns.

영어경시대회 측정도구의 과업별 적절성 검증 및 개선 방안

송미정,박용예 서울대학교 어학연구소 2002 語學硏究 Vol.38 No.1

This study analyzes the validity of the tasks in Test of oral Proficiency (TOP) and Test of Writing Proficiency (TWP) as means of evaluating advanced-level high school students' English proficiency in a nation-wide English contest. For this purpose, both quantitative and qualitative analyses were conducted. The results show that all the tasks are statistically valid except for Task Ⅰ (Answering simple questions) for Group Ⅱ (who have stayed overseas longer than one year). The examination of students' recorded responses for TOP reveals how differently Groups Ⅰ and Ⅱ responded to the tasks and what can be done to improve task questions. Based on the findings, we suggest that for TOP, the current format be maintained for Group Ⅰ (who have stayed overseas shorter than one year). For Group Ⅱ, however, a modified use of TOP or its replacement with interviews is recommended. For TWP, since Task Ⅰ (Summary) and Ⅳ (Free writing) among the four tasks are very effective to measure students' writing proficiency, we recommend to use these two tasks alone instead of using all four of the tasks. We also suggest that a thorough training and detailed guidelines be provided for raters.