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이찬용,김소영,김류련,우영은 충남대학교 생물공학연구소 2005 생물공학연구지 Vol.11 No.2
Bioluminescence refers to the process of visible light emission in living organism mediated by an enzyme catalyst. A common lux gene organization includes the genes coding for the bacterial luciferase subunits (luxAB) and the fatty acid reductase polypeptides (luxCDE) responsible for biosynthesis of the aldehyde substrate for the luminescence reaction. Additional genes, involved in regulation of flavin binding and metabolism, were found in the specific luminescent bacteria. Light intensity per cell is highly dependent on cellular growth owing to the spectacular autoinduction of luminescence at high cell density. Therefore, the bacterial lux system is of particular interest as it can serve as an excellent model for more general signal transduction system called quorum sensing regulation. In addition, the potential applications of bacterial bioluminescence as a safe, rapid, and sensitive sensor for a wide variety of compounds and metabolic process were also described in this review paper.
최근 국내 유입 Vibrio cholerae균 및 1995년도 국내 집단 발생 콜레라의 역학적 양상
김호훈,신영학,강연호,유천권,박미선,김동술,유재연,전정훈,이복권,박기덕,김동진,정태화,이종구,박기동,김상순,이동모,김문식,조병륜 대한감염학회 1996 감염 Vol.28 No.6
목적 : 1992년 이후 국내에 유입, 확인되는 V. cholerae 균은 현격한 증가 추세를 보이고 있으며, 1995년도 국내 집단 발생 콜레라의 역학적 양상이 종래의 경우와 상이한 바 있어 최근의 콜레라균 유입상황과 국내 역학적 특성을 정리하여 향후의 방역 대책에 참고로 하기 위하여 본 연구를 시행하였다. 방법 : 국내 유입 V. cholerae 균은 세균학적 방법에 의해 1986-1995년 사이에 확인된 균주를 대상으로 분석하였고, 국내 집단발생 콜레라는 1995년도에 내국인 거주자에게서 진성 콜레라 환자로 구명된 환자를 대상으로 역학적 양상을 살펴보았다. 결과 : 국내 유입 V. cholerae균은 1992년 이후 1995년 사이에 계절과 무관하게 뚜렷한 증가 추세를 보였다. 1995년도 콜레라 국내 집단 발생의 역학적 양상은 초발 환자가 인지되면서 단기간 사이에 지역적 연고가 없는 다양한 지역에서 콜레라 환자 발생이 확인되었다. 환자 발생 지역 내에서는 비교적 소규모 환자 발생에 그쳤고 진성 콜레라 환자로 확인된 환자 중 사망자는 없었으며 남성에서 여성보다 발병율이 높았다. 결론 : V.cholerae O1균의 국내 유입이 최근 현격히 증가되었고, 1994년에는 V. cholerae O139균의 국내유입이 처음으로 확인되었다. 콜레라 환자의 국내 집단 발생 위험은 1992년 이후 증가되었으며 1995년도의 집단 환자 발생은 지역적 연고가 없는 다양한 지역에서 비교적 단기간에 사망자 없이 소규모의 환자 발생양상을 보였다. Background: Imported Vibrio cholerae strains have increasingly been found since 1992 and the pattern of recent cholera epidemic outbreak in Korea showed a little difference from that of former epidemic outbreak. Methods: We had collected suspected V. cholerae isolates from Quarantine Stations or Public Health Laboratories, which were identified as V. cholerae O1 or O139. Epide miological analysis was made on the base of field surveys. Results: During the period from 1986 through 1995, 138 V. cholerae strains were imported. Nearly 85.5% of the strains (118 strains) were imported within recent 4 years from 1992 to 1995. One hundred and twenty-eight strains were E1 Tor-Ogawa type V. cholerae O1, 6 strains were V. cholerae O139 and 4 strains were E1 Tor-Inaba type V. cholerae O1. Of 138 strains, 71 strains were isolated from the airplane toilet swab and 67 strains from the passengers who entered into Korea after international travel. From 1993 to 1995, 101 strains were imported from the Southeast Asian countries; including Thailand(65), Indonesia(17), Philippines(10), Hongkong(6) Singapore(2) and Vietnam(1). During the cholera epidemic in 1995, 68 patients with V. cholerae O1 (E1 Tor-Ogawa) were found. Oneset of the index-case occurred on August 26th and the last on September 13th. Geographically it occurred in Incheon city(25), Chungcheongnam do(25), Kangwon do(6), Kyungsangbuck do(5), Kyunggi do(4), Daejeon city(2) and Pusan city(1). Of total 68 cases, 39 were male. Cases were found in all age groups, but it was more common in elderly groups. During the epidemic, E1 Tor-Ogawa type V. cholerae O1 strains were also isolated from the coastal sea water of Kanghwa kun where the epidemic outbreak occurred. Conclusions: In Korea, the imported V. cholerae O1 and V. cholerae O139 strains have increasingly been found without seasonal variation since 1992. Cholera epidemic outbreak in 1995, occurred simultaneously at many different local areas within a short period without mortality.
Kim, Young-Jon,Kim, Byoung-Ryun,Ryu, Jae-Suk,Lee, Gyeong-Ok,Kim, Hak-Ryul,Choi, Keum-Ha,Ryu, Jae-Won,Na, Kyoung-Suk,Park, Min-Cheol,So, Hong-Seob,Cho, Ji-Hyun,Park, Do-Sim Blackwell Scientific Publications 2017 International journal of gynecological cancer Vol.27 No.2
<B>Objective</B><P>Heterogeneous nuclear ribonucleoprotein A1 (HNRNPA1), serine/arginine-rich splicing factor 1 (SRSF1), and SRSF3 are splicing regulators associated with oncogenesis. However, the alterations of SF proteins and their diagnostic values in cervical cancer are unclear. To apply SFs clinically, effective marker selection and characterization of the target organ properties are essential.</P><B>Materials and Methods</B><P>We concurrently analyzed HNRNPA1, SRSF1, SRSF3, and the conventional tumor markers squamous cell carcinoma antigen (SCCA) and carcinoembryonic antigen (CEA) in cervical tissue samples (n = 127) using semiquantitative immunoblotting. In addition, we compared them with p16 (cyclin-dependent kinase inhibitor 2A [CDKN2A]), which has shown high diagnostic efficacy in immunohistochemical staining studies and has been proposed as a candidate protein for point-of-care screening biochemical tests of cervical neoplasia.</P><B>Results</B><P>HNRNPA1, higher molecular weight forms of SRSF1 (SRSF1-HMws), SRSF3, CEA, and p16 levels were higher (<I>P</I> < 0.05) in cervical carcinoma tissue samples than in nontumoral cervical tissue samples. However, the levels of SRSF1-Total (sum of SRSF1-HMws and a lower molecular weight form of SRSF1) and SCCA, a commonly used cervical tumor marker, were not different between carcinoma and nontumoral tissue samples. In paired sample comparisons, HNRNPA1 (94%) showed the highest incidence of up-regulation (carcinoma/nontumor, >1.5) in cervical carcinoma, followed by p16 (84%), SRSF1-HMws (69%), SRSF3 (66%), CEA (66 %), SCCA (32%), and SRSF1-Total (31%). HNRNPA1 (92%) and p16 (91%) presented the two highest diagnostic accuracies for cervical carcinoma, which were superior to those of SRSF3 (75%), SRSF1-HMws (72%), CEA (72%), SCCA (59%), and SRSF1-Total (55%).</P><B>Conclusions</B><P>Our results identified that HNRNPA1 is the best diagnostic marker among the SFs and conventional markers given its excellent diagnostic efficacy for cervical carcinoma, and it has a p16-comparable diagnostic value. We suggest that HNRNPA1 is an additional effective target protein for developing cervical cancer detection tools.</P>
Nondiabetic kidney diseases in type 2 diabetic patients
( Ye Jin Kim ),( Yoo Hyung Kim ),( Ki Dae Kim ),( Kang Ryun Moon ),( Jae Ho Park ),( Bo Mi Park ),( Hyewon Ryu ),( Dae Eun Choi ),( Ki Ryang Na ),( Kwang Sun Suh ),( Kang Wook Lee ),( Young Tai Shin ) 대한신장학회 2013 Kidney Research and Clinical Practice Vol.32 No.3
Background: The aim of this study was to evaluate the clinical characteristics of nondiabetic nephropathy in type 2 diabetes mellitus patients and to find a clinical significance of renal biopsy and immunosuppressive treatment in such a patient. Methods: Renal biopsy results, clinical parameters, and renal outcomes were analyzed in 75 diabetic patients who underwent kidney biopsy at Chungnam National University Hospital from January 1994 to December 2010. Results: The three most common reasons for renal biopsy were nephrotic range proteinuria (44%), proteinuria without diabetic retinopathy (20%), and unexplained decline in renal function (20.0%). Ten patients (13.3%) had only diabetic nephropathy (Group I); 11 patients (14.7%) had diabetic nephropathy with superimposed nondiabetic nephropathy (Group II); and 54 patients (72%) had only nondiabetic nephropathy (Group III). Membranous nephropathy (23.1%), IgA nephropathy (21.5%), and acute tubulointerstitial nephritis (15.4%) were the three most common nondiabetic nephropathies. Group III had shorter duration of diabetes and lesser diabetic retinopathy than Groups I and II (P¼0.008). Group II had the lowest baseline estimated glomerular filtration rate (P¼0.002), with the greatest proportion of renal deterioration during follow-up (median 38.0 months, Po0.0001). The patients who were treated with intensive method showed better renal outcomes (odds ratio 4.931; P¼0.01). Absence of diabetic retinopathy was associated with favorable renal outcome in intensive treatment group (odds ratio 0.114; P¼0.032). Conclusion: Renal biopsy should be recommended for type 2 diabetic patients with atypical nephropathy because a considerable number of these patients may have nondiabetic nephropathies. And intensive treatment including corticosteroid or immunosuppressants could be recommended for type 2 diabetic patients with nondiabetic nephropathy, especially if the patients do not have diabetic retinopathy.
한국 여성에서 자연 조기 분만과 Interleukin-1β 및 Interleukin-1 receptor antagonist 유전자 다형성의 연관성
김세련 ( Se Ryun Kim ),박영준 ( Young Jun Park ),류기영 ( Ki Young Ryu ),김종욱 ( Jong Wook Kim ),송은섭 ( Eun Seob Song ),이병익 ( Byung Ick Lee ) 대한산부인과학회 2007 Obstetrics & Gynecology Science Vol.50 No.1
목적: 조산은 영아 사망 및 이환의 중요한 인자이나 원인 및 기전이 여전히 명백하지 않다. 최근 여러 원인 중 염증과 관련있는 cytokine의 유전자 다형성과의 관계에 대한 연구가 활발하여 한국 여성에서도 interleukin-1β및 interleukin-1 receptor antagonist의 유전자 다형성의 차이가 있는지 알아보고자 한다. 연구 방법: 정상 분만군 33명과 조산군 27명을 대상으로 IL-1β+3953과 IL-1 Ra의 유전자 다형성에 대한 중합 효소 연쇄반응 및 제한 절편길이 다형성을 조사하였다. 결과: 정상 분만군과 조산군의 연령, 산모 체중, 임신력 등은 차이를 보이지 않았으나 제태 연령, 출생 체중은 유의한 차이를 보였다 (p<0.01). IL-1β+3953은 135 bp/114 bp와 249 bp/249 bp 두 가지 유형을 보였는데 유전자형의 빈도 및 대립 유전자 빈도가 유의한 차이를 보이지 않았다 (p=0.182, p=0.162). IL-1 Ra는 410 bp, 240 bp, 500 bp, 325 bp, 595 bp의 다섯 가지 유전자형을 보였는데 유전자형의 빈도 및 대립 유전자 빈도 모두 유의한 차이를 보이지 않았다 (p=0.101, p=0.094). 결론: IL-1β+3953과 IL-1 Ra는 정상 분만과 조산군 간의 유전자 다형성의 차이를 보이지 않았다. Objective: Preterm birth is an important cause of infant death and morbidity but its pathophysiology still remains to be clear. The associations between preterm birth and the polymorphism of interleukin-1β gene and interleukin-1 receptor antagonist gene have been suspected. And ethnic variations in the polymorphism of the genes were also reported. We decided to study polymorphisms of interleukin-1β+3953 and interleukin-1 receptor antagonist in Korean pregnant women. Methods: Patients were divided into 2 groups. Group 1 is the control group of 33 subjects with uncomplicated term delivery : group 2 is the case group of patients who had spontaneous preterm delivery. Polymorphisms were determined by polymerase chain reaction and restriction fragment length polymorphism analysis. Results: There were significant differences in gestational age and birth weight between two groups (P<0.001). No significant differences were found in genotypic frequencies and allelic frequencies in interleukin-1β+3953 between two groups. Interleukin-1 receptor antagonist gene had 5 alleles and the most frequent allele was IL1RN*1 (410bp), 66.7% in control group and 77.8% in case group. And significant differences were not found in genotypic frequencies and allelic frequencies between two groups, too. Conclusion: There were no significant differences in polymorphisms in interleukin-1β +3953 and interleukin-1 receptor antagonist between term delivery group and preterm birth group.