Aspergillus 속 미생물에 의한 발효비지의 항비만 효과

이상일(Lee, Sang-Il),이예경(Lee, Ye-Kyung),김순동(Kim, Soon-Dong),임종환(Lim, Jong-Hwan),서주원(Seo, Ju-Won),이인애(Lee, In-Ae) 한국산학기술학회 2013 한국산학기술학회논문지 Vol.14 No.11

고지방식이로 비만을 유도한 ICR 생쥐를 동물 모델로 하여 7종의 Aspergillus속 미생물로 발효시킨 발효비지 의 항비만 효과를 조사하였다. 수컷 ICR 생쥐에 5주간 고지방 식이를 실시하여 비만을 유도하고 고지방식이에 발효 비지 2%를 첨가하여 6주 동안 사육하였으며, 체중, 섭취량, 혈청 총콜레스테롤, 중성지질, Alanine transaminase과 high-density lipoprotein cholesterol, 간성 glutathione과 lipid peroxide를 측정하였다. 체중의 경우에 AE4 발효 비지를 식이한 군에서 가장 많이 감소 (31.33%, P<0.05)하였고 간성 GSH와 ALT증가로 고지방혈증이 저해되었다. 이상의 결 과로부터 Aspergillus속 미생물로 발효시킨 발효비지는 항비만 효과가 있는 것으로 나타났으며, 나아가 인체에서도 유 의한 비만개선 작용을 기대할 수 있을 것으로 사료된다. The anti-obesity effect of soybean curd residues (biji) fermented by seven Aspergillus spp. was investigated with obese ICR mice fed a high-fat diet. After inducing obesity by feeding high-fat diet for 5 weeks, animals were fed with a high fat diet supplemented with 2% fermented soybean curd residues for 6 weeks. Total cholesterol, triglyceride, Alanine transaminase, high-density lipoprotein cholesterol, hepatic content of glutathione and lipid peroxide were determined. In the case of body weight, AE4 group showed most prominent decrease (31.33%, P<0.05) and increase of hepatic GSH and ALT demonstrated hyperlipidemia inhibition. From the results, it is concluded that soybean curd residues fermented by Aspergillus spp. has anti-obesity effect and it is thought that fermented soybean curd resides can reduce obesity in human significantly.

[논문] 2009개정 교육과정에 따른 물리Ⅰ교과서와 영국의 Advancing Physics에 제시된 삽화에 대한 학생의 인식

서주옥,이지애,김영민 釜山大學校 師範大學 科學敎育硏究所 2011 科學敎育硏究報 Vol.38 No.-

Students recognize illustrations before letters when they look at textbooks. Students find some illustrations difficult to understand, not because there is something wrong with the illustration itself but because information has not been provided or presented in an effective way. For this reason, Illustrations are very important for students to build up the concepts of what they are learning. For the purpose of the study, we have made 12 questions with selected 9 illustrations included in Information and Communication unit of the Physics Ⅰ textbooks according to the revised national curriculum in 2009 and Advancing Physics of UK and then we conducted a survey with 360 students of high school and 22 teachers of science. This study intends to recognition the contents of physicsⅠ textbooks, focusing on the chapter of Information and Communication. For this, a questionnaire on the three types of illustrations was prepared to find out what kind of illustration students thought was easy to understand and why they chose it. Based on the answers to the questionnaire, the conditions for clear and effective communication of textbook information through illustrations were found, by analyzing the advantages and disadvantages of some types of illustrations. In this study, illustrations students thought difficult were picked out be easier and clearer to understand to see which of them are more helpful for students. The features of illustrations easy for students to understand well are as follows: First, the essence of improving illustrations is that the contents should be concise and brief in order not to confuse students. The the illustration should also include each step of the process through which the result was achieved, instead of simply showing the result. Second, the illustration should not be difficult to externally visible; When high school students feel frustrated while trying to understand the illustration, they tend to give up rather than ponder more to get to the meaning. Third, no matter how difficult illustrations are, some good clues and explanations about them make students try to understand what they mean positively rather than lose interest.

한국전통무용 기록·보존에 관한 방법론 연구 : 영상매체의 활용을 중심으로 especially in connection with using the media of film of video

이애경,이애주 師範大學 體育硏究所 1992 서울大學校 體育硏究所論集 Vol.13 No.1

In Korea, there are many traditional dances those have been handed down since old times. These Korean traditional dances must be handed over the future generations like as those from ancient times to present days. This study is an examination for the purpose of seeking after the method of transmitting Korean traditional dances from generation to generation, and it aims at a positive proposal about putting the film by video media to pratical use. In that point of view, I have investigated some methods for putting Korean traditional dances on record. And the conclusions are these. 1. The most important method for transmitting Korean traditional dances from generation is the means of teaching and learning from a person to a person directly, in other words the means of imparting by lips and heart. 2. And another important method for transmitting Korean traditional dance from generation to generation is the means of scoring by letters or figures that is named generally ‘notation ’. But in that course some supplementary are in need inevitably. 3. If we want to reflect the special quality of dance in relation to the character of time and space, we must pick up the method of using film or video media together with the old notation. 4. The subject of filming must be choosed after rating from the point of historical value, artistic value, and realistic value. And for combining the side of Art with the side of document, we must have an interest in some suitable camera techniques.

중합효소연쇄반응을 이용한 도말양성 폐결핵 환자에서의 결핵균 DNA 검출방법에 관한 연구

이종진,김애경,조해정,한표성,홍석철,김주옥,김선영 충남대학교 의과대학 지역사회의학연구소 1994 충남의대잡지 Vol.21 No.2

Polymerase chain reaction (PCR) is the most sensitive and rapid method for the diagnosis of M.tuberculosis infection. Many reports have shown different methods for mycobacterial DNA extraction, but revealed many differences in simplicity and time-consumption. We studied which method was the best among 7 different ones for DNA extraction from 32 smear-positive sputa, using PCR of targeting 123bp DNA fragment of IS6110. The methods were the following : SDS-Microwave oven method, NaOH Lysis method, Triton X-100, Proteinase K method, Lysis buffer method. SDS-Proteinase K method, Bead Beater method, and Bead Beater/CTAB method. The amplification products were identified by electrophoresis on ethidium bromide-stained agarose gels, and confirmed by restriction enzyme, Southern blot hybridization, and DNA sequencing. All 32 cases were positive (100%) by the 7 different methods. It took 1 hour or more to detect DNA in SDS-Microwave oven method, NaOH Lysis method, and Bead beater/CTAB method. It took 2 hours or less in the others. SDS-Proteinase K method and Bead Beater/CTAB method were simpler and more convinient than the others. These results suggest that SDS-Proteinase K method and Bead Beater/CTAB method might be better than the others for the extraction of mycobacterial DNA in terms of simplicity and time-consumption, although all the methods were sensitive.

중세 남녀 속옷의 특성

김주애,이연희 服飾文化學會 2003 服飾文化硏究 Vol.11 No.2

This study analyzes how social environment infuenced underclothes and characteristic which underclothes have is associated with the outer garment and the underclothes in the Middle Ages. As a result, I can get the next conclusion First, throughout the Middle Ages, body was involved with the sense of same and sin influenced by the religious austerity. This mentality was applied to the underclothes. As underclothes are not generally revealed, it is recognized to be not important but inefficient. Second, in the late Middle Ages, as the outer garment reveal the body line, the underclothes are affected by it. So men's and women's distinction in the outer garment is applied to the underclothes. In the second quarter of the 14C, class distinction and sexual attraction certainly present on the outer garment but are not applied to the underclothes. The conception that underclothes might express the spirit of the changing times, in sympathy with the outer garment, did not enter the medieval mind. Third, the function of protect the body is emphasized the underclothing in the Middle Ages, whereas it was important to identify rank sign in the histroy of underclothes. Throughout the Middle Ages the underclothing of both sexes is emphasized utilitarian in function rather than class distinction and sexual attraction. But women exposed their body line in thin chemise to represent sexual attraction in the late Middle Ages.

천연자원의 간기능 보호 효과에 관한 연구 : Benzo(a)pyrene에 의해 유도된 간기능 장해에 미치는 강활의 효과 The Effect of Angelica koreana on Benzo(a)pyrene Induced Hepatotoxicity

윤수홍,이송애,박은주,이주영 효성여자대학교 부설 한국환경위생연구소 1992 환경위생연구 Vol.2 No.1

민간에서 주로 사용되는 약용식물들 중 간독성의 발현 저해 및 간기능 보호 효과가 우수한약물을 찾아 임상적인 약효를 밝혀보고자 하는 실험과정으로 탄소화물의 불완전 연소 및열분해에 의해 생성되는 간장해 물질인 benzo(a)pyrene으로 유도한 rats의 간독성 발현에미치는 강활 수침액의 예방 및 치료 효과를 실험하여 다음과 같은 결과를 얻었다. 1. 강활 수침액의 투여는 B(a)P투여로 현저하게 증가된 혈청 및 간장의 AST, ALT, LDH,ALP 활성의 유의성 있게 감소시켰다. 2. B(a)P투여로 증가한 혈청 total cholesterol 및 phospholipid 함량은 강활 수침액의 투여로감소시킬 수 있었다. 3. 강활의 투여는B(a)P에 의한 간독성 발현을 유의성 있게 감소시켰으며 그 효과는 전처리가후처리에 비해 우수하였다. The present study was undertaken to compare the pharmacologicalactivities of crude Angelica koreana reported with the clinical uses in the orientalmedicine. Crude angelica. used for the treatment of common cold, headache, neu-ralgia and arthralgia. Therefore we tested the effects of Angelica water extract on the liver-protec-live activities in the rats. The results obtained from liver microsomal enzymeassay, measurement of serum and liver alanine' aspartate aminotransferase andlipid accumulation indicated that angelica extract showed significant liver-protec-tive activities against benzo(a)pyrene poisoning.

보건소 건강증진사업 수행과정의 질 평가

서영준,정애숙,박태선,김주경,박남수,이희원 韓國保健敎育.健康增進學會 2003 보건교육건강증진학회지 Vol.20 No.1

This study purports to assess the process quality of health promotion programs at public health centers. The sample used in this study consisted of 242 public health centers in South Korea. Data was collected with self-administered questionnaires between September. 3rd to September. 23rd in 2002. The 95 returned questionnaires were analyzed by SPSS 11.0 version. The major findings are as follows; There is a need to improve (1) a procedure for reviewing and analyzing strategic planning, (2) the quality of the staff in charge of program management, (3) the function of planning and training for monitoring and evaluation, (4) the ability of program managers in advising and consulting with clients, and (5) a procedure for organizing resources and information. In conclusion, in order to improve the process quality of health promotion programs at public health centers, integrated support from various institutions such as public health centers, community, regional and national health authorities and administrative departments is required.

Benzo(a)pyrene에 의해 유도된 간기능 장해에 미치는 세신 추출액의 영향

정영건,권오진,윤수홍,이송애,이주영 효성여자대학교 부설 한국환경위생연구소 1993 환경위생연구 Vol.3 No.1

탄소화합물의 불완전 연소 및 열분해에 의해 생성되는 다환방향족 탄화수소로 간 실질세포에서 cytochrome P_450에 의해 산화되는 간장해 물질인 benzo(a)pyrene으로 유도한 rats의간독성 발현에 미치는 세신 수침액의 예방 및 치료 효과를 실험한 결과 세신 수침액의 투여는B(a)P 투여로 현저하게 증가된 혈청 및 간장의 transaminase 활성을 유의성 있게 감소시켰으며 그효과는 조직에서 보다 우세하였다. 그러나 세신 수침액이 B(a)P투여로 증가한혈청 total cholesterol과 phospholipid 함량을 감소시킬 수는 있었지만 그 효과는 약하여 지질대사에는 세신의 작용이 미미할 것으로 보인다. Effects of Asiasari Radix, which is herbal drug used frequentlyin the oriental prescriptions, water extract on the liver-protective activities wereinvestigated in the rats. Asiasari Radix water extract, when administered intothe gastric intubation, produced liver-protective effect against benzo(a)pyreneinduced liver damage. The results obtained from enzyme assay, measurement of serum and liveralanine aspartate aminotransferase(AST, ALT) and lipid composition indicatedthat Asiasari water extract showed significant liver-protective activities againstbenzo(a)pyrene-induce hepatotoxicity.

열공형과 비열공형 피질하 혈관성 치매에서 위험인자의 차이에 관한 비교 연구

배희준,정지향,유경호,나덕렬,김상윤,최경규,양동원,손의주,이상도,김재우,박경원,김응규,이재홍,박미영,한일우,함동석,최문성,하충건,최성혜,이애영,이병철,한설희 대한치매학회 2003 Dementia and Neurocognitive Disorders Vol.2 No.2

Backgrounds and Objectives: Vascular dementia is a group of dementing disoders arising from various stroke syndrome. Among these. subcortical ischemic vascular dementia (SIVD) is regarded as a relatively distinct clinical entity. However, MRI patterns of SIVD are not homogenous. In some patients, lacunes are dominant, and in others, subcortical white matter changes are. This study was designed to compare risk factor profiles between SIVD with and without multiple lacunes. Methods: We divided 47 subjects (22 males, mean age. 68 years) recruited from VADAPET (Multicenter Trial For Evaluation Of The Changes In the PET Images Of Subcortical Vascular Dementia Patient) study into two groups one with more than 5 lacunes in deep gray matter (lacune group) and the other with 5 or less(non-lacune group) Clinical characteristics and laboratory findings of two groups were compared. Results: Nineteen of 47 patients (40%) belonged to the lacune group. The lacune and non-lacune groups d d not differ in the following variables: age, hypertension, diabetes mellitus, hyperlipidemia heart disease, history of stroke or TIA, history of trauma or major surgery, family history of hypertension stroke, or dementia, age at diagnosis of dementia, body mass index, white blood cell count, ESR, CRP, fibrinogen, hemoglobin A1C, total cholesterol. LDL cholesterol creatinine, proteinuria, glucosuria, and microhematuria. However, male sex, smoking alcohol. hemoglobin, and HDL cholesterol were possibly associated more with lacune group SIVD than with non-lacune group (p<0 1) Multivariate analyses revealed that smoking, hemoglobin, and HDL cholesterol were independent predictors of SIVD with multiple lacunes Conclusion: Our study suggests that SIVD with multiple lacunes may be significantly different in smoking habits hemoglobin, and HDL cholesterol from SIVD without multiple lacunes.

The irony benign tumor of Aggressive angiomyxoma

( Wan-ju Kim ),( Hee-jung Lee ),( Yeon-ji Lee ),( Yoon-kyoung Lee ),( Jin-seok Suck ),( In-ae Cho ),( Jeong-kyu Shin ),( Won-jun Choi ),( Soon-ae Lee ),( Jong-hak Lee ),( Won-young Paik ) 대한산부인과학회 2016 대한산부인과학회 학술대회 Vol.102 No.-

The aggressive angiomyxoma is known for benign tumor because of non-metastasizing and not invading other organs, but it have high recurrence rate. Why it called as aggressive. A 23 year-old-woman who suffered from labial mass came outpatient clinic. Her chief complaint was palpable vulva mass. She had no underlying diseases until knew about it. She was no pain and redness. Her first impression was Bartholin's cyst and we prepared Marsupialization. Used local anesthesia by Lidocain, surgical exision was done. Surprisingly, the result of biopsy was suspicious aggressive angiomyxoma. There have tendency of local recurrence more often. Regrettably, there was no proven treatment of medicine. Some people have tried to effort treatment by chemotherapy. Consequently, the patient need to have a periodic checkup for recurrence the benign myxoid tumor.