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Determination of Ceftiofur in Bovine Serum by liquid Chromatography-Electrospray Mass Spectrometry
임종환,장범수,박병권,윤효인,Lim, Jong-hwan,Jang, Beon-su,Park, Byung-kwon,Yun, Hyo-in The Korean Society of Veterinary Science 2003 大韓獸醫學會誌 Vol.43 No.3
This study was aimed to develop a more rapid, simple and sensitive method to determine ceftiofur in bovine serum using LC/MS with electrospray interface. Separation was achieved on the Nova-Pak $C_{18}$ reverse phase column. The mobile phase consisted of 0.1% acetic add in water (A) and acetonitrile (B) and gradiently flowed at the rate of 0.4 mL/min. As a result of analysis of blank muscle samples, matrix interference was not shown. Limit of detection and limit of quantitaion was 5 ng/g and 20 ng/g, respectively. The values of precision and recovery satisfied the guideline of NVRQS. The precision and recovery developed in this method are suitable and sensitive to determine the concentration of ceftiofur in the bovine serum. These results could be applied for the confirmation and quantification in the biofluid.
임종환,장범수,박병권,윤효인,Lim, Jong-hwan,Jang, Beom-su,Park, Byung-kwon,Yun, Hyo-in The Korean Society of Veterinary Science 2003 大韓獸醫學會誌 Vol.43 No.3
The objective of this study was to investigate the residue depletion of cephaalexin in the flounder (Paralichthys olivaceus) after multiple oral administrations and to establish the appropriate withdrawal time for edible tissues. A highly sensitive and specific method for the determination of cephalexin in the serum of flounder by LC/MS was developed and validated. Mean recoveries from serum were 87.2% (ranged from 81.2% to 94.5%) for cepalexin. Recovery and precision met the criteria for the guideline of residual analysis of veterinary drugs by the National Veterinary Research and Quarantine Service (NVRQS) in Korea. The limit of detection and limit of quantitation of cephalexin were 10 ng/ml and 50 ng/ml, respectively. Residual levels of cephalexin in muscle samples were estimated with 95% tolerance limit and 95% confidence to fall below the MRL after a withdrawal time of 4 days and 5 days for the 40 and 160 mg/kg/day, respectively.
고령화시대 실버타운의 선호도 및 입주의향 분석에 관한 연구
임종환 ( Lim Jong-whan ),엄수원 ( Eum Soo-won ) 한국지역사회발전학회(구 한국지역사회개발학회) 2019 地域社會開發硏究 Vol.44 No.1
This study quantified the perception of individuals through a survey to investigate the social recognition and preference of silver town. The questionnaire expresses individual thoughts, and objectification is given priority in quantifying it. Therefore, reliability and validity were tested to apply the results of this study in general. As a result of the reliability analysis, the Cronbach's α coefficient value was 0.734 in the cognitive factor and 0.845 in the internal characteristic factor, showing high reliability. The KMO value of validity test was 76.213∼1825,656, which was smaller than p<0.01, and the validity was confirmed. The factors affecting the preference of silver town were cognition, location characteristics, physical characteristics, and program characteristics. Factors affecting the intention to move were analyzed by cognition, location, physical, internal, and program characteristics. The silver town and the silver industry are the tasks we have to prepare, and we must create conditions in which the private sector can actively participate. Role sharing is required in each area and it should be studied from the viewpoint of welfare so that it becomes a developmental view in the future.
무인모선기반 무인잠수정의 3차원 위치계측 기법에 관한 연구
임종환(JONG-HWAN LIM),강철웅(CHUL-UNG KANG),김성근(SUNG-KYUN KIM) 한국해양공학회 2005 韓國海洋工學會誌 Vol.19 No.2
A 3-D localization method of an autonomous underwater vehicle (AUV) has been developed, which can solve the limitations of the conventional localization, such as LBL or SBL that reduces the flexibility and availability of the AUV. The system is composed of a mother ship (small unmanned marine prober) on the surface of the water and an unmanned underwater vehicle in the water. The mother ship is equipped with a digital compass and a GPS for position information, and an extended Kalman filter is used for position estimation. For the localization of the AUV, we used only non-inertial sensors, such as a digital compass, a pressure sensor, a clinometer, and ultrasonic sensors. From the orientation and velocity information, a priori position of the AUV is estimated by applying the dead reckoning method. Based on the extended Kalman filter algorithm, a posteriori position of the AUV is, then, updated by using the distance between the AUV and a mother ship on the surface of the water, together with the depth information from the pressure sensor.