虹彩 體質 分析에서 心腎結合組織의 虛弱 體質과 高血壓 家族歷과 관련성 硏究

金鐘牛,黃祐準,琴坰樹,李始炯,李宗淳,都金錄,趙在運,趙州掌,金鐘煜 한국전통의학연구소 2002 한국전통의학지 Vol.12 No.1

Iridology, developed more than 100 years ago, is the diagnosis of medical conditions through noting irregularities of the pigmentation in the iris The purpose of this study is to compare interrelation between CVA, hypertension family history and cardio-renal connective tissue in Iris Constitution through iris constitution examination. The subjects consist of 114 patients who were examined in Iris Constitution at Chonju Oriental Medicine Hospital attached to Wonkwang Univ. from September. 1St. 2000 to August. 31th. 2002. 1. In the distribution of Iris Constitution, among of 79cases, There are neurogenic type 13cases, abdominal connective tissue weakness constitution 31 cases, cardio-renal connective tissue weakness constitution 6 cases, cholesterol 7 cases and others 22 case in control group. 2. There are familial history of CVA and hypertention 32 cases, no familial history of CVA and hypertention1 3 cases, in 35cases of experimental group. These results imply that there is meaningfulness of interrelation between cardio-renal connective tissue weakness constitution and. CVA, hypertention.

Antimutagenic Effects of Ginsenoside Rb$_1$, Rg$_1$ in the CHO-K1 Cells by Benzo[a]pyrene with Chromosomal Aberration Test and Comet Assay

Kim, Jong-Kyu,Kim, Soo-Jin,Rim, Kyung-Taek,Cho, Hae-Won,Kim, Hyeon-Yeong,Yang, Jeong-Sun The Korean Society of Toxicogenomics and Toxicopro 2009 Molecular & cellular toxicology Vol.5 No.2

The usage and types of chemicals are advancing, specializing, large-scaled increasing, and new chemical exposed workers are concerning to occupational disease. The generation of reactive oxygen in the body from carcinogen, mutation and DNA damage in cancer is protected by natural antioxidants (phytochemicals) with antimutagenic effect. There were many reports of ginsenoside Rb$_1$, Rg$_1$ grievances of the genetic mutation to suppress the effect confirm the genetic toxicity test with chromosomal aberration test and the Comet (SCGE) assay confirmed the suppression effect occurring chromosomal DNA damage. We had wanted to evaluate the compatibility and sensitivity between the chromosomal aberration (CA) test and the Comet assay. We used the CA test and Comet assay to evaluate the anti-genotoxicity of ginsenoside Rb$_1$ and Rg$_1$, in CHO-K1 (Chinese hamster ovary fibroblast) cell in vitro, composed negative control (solvent), positive control (benzo[a]pyrene), test group (carcinogen+variety concentration of ginsenoside) group. The positive control was benzo[a]pyrene (50 $\mu$M), well-known carcinogen, and the negative control was the 1 % DMSO solvent. The test group was a variety concentration of ginsenoside Rb$_1$, Rg$_1$ with 10$^{-8}$%, 10$^{-6}$%, 10$^{-4}$%, 10$^{-2}$%, 1%, 10%. In chromo-somal aberration test, we measured the number of cells with abnormally structured chromosome. In Comet assay, the Olive tail moment (OTM) and Tail length (TL) values were measured. The ratio of cell proliferation was increased 8.3% in 10$^{-8}$%, 10$^{-6}$%, 10$^{-4}$%, 10$^{-2}$%, 1%, 10% Rb$_1$ treated groups, and increased 10.4% in 10$^{-10}$%, 10$^{-8}$%, 10$^{-6}$%, 10$^{-4}$%, 10$^{-2}$%, 1% Rg$_1$ treated groups. In the CA test, the number of chromosomal aberration was decreased all the Rb$_1$ and Rg$_1$ treated groups. In the Comet assay, the OTM values were decreased in all the Rb$_1$ and Rg$_1$ treated groups. To evaluate the compatibility between CA and Comet assay, we compared the reducing ratio of chromosomal abnormalities with its OTM values, it was identified the antimutagenicity of ginsenoside, but it was more sensitive the CA test than the Comet assay. Ginsenoside Rb$_1$ and Rg$_1$ significantly decrease the number of cells with chromosomal aberration, and decrease the extent of DNA migration. Therefore, ginsenoside Rb$_1$, Rg$_1$ are thought as an antioxidant phytochemicals to protect mutagenicity. The in vitro Comet assay seems to be less sensitive than the in vitro chromosomal aberration test.

Serum TSH Level in Healthy Koreans and the Association of TSH with Serum Lipid Concentration and Metabolic Syndrome

( Yeo Kyung Lee ),( Jo Eun Kim ),( Hyun Ju Oh ),( Kyung Sun Park ),( Soo Kyung Kim ),( Seok Won Park ),( Moon Jong Kim ),( Yong Wook Cho1 ) 대한내과학회 2011 The Korean Journal of Internal Medicine Vol.26 No.4

Background/Aims: The proper treatment of subclinical hypothyroidism and the normal range of serum thyroid stimulating hormone (TSH) concentration are intensely debated. However, few reports have investigated TSH concentrations in Asian ethnic groups. Therefore, the present study was designed to define the TSH reference range in a Korean population and to investigate the metabolic significance of TSH concentration. Methods: We enrolled patients who underwent medical examination at the CHA Bundang Medical Center. Anthropometric data were evaluated, and serum TSH, free T4, and lipid profiles were assayed. Results: A total of 7,270 subjects were included. Mean TSH concentration of the study population was 1.82 ± 0.95 mU/L, and we observed a sex-related difference in TSH concentration (male, 1.67 ± 0.87 mU/L; female, 2.02 ± 1.01 mU/L; p < 0.01). When the 2.5 and 97.5 percentiles were calculated, 95% TSH reference limits were 0.52-4.29 mU/L. TSH concentration was higher in elderly subjects, during winter, in postmenopausal women, and in obese males. Moreover, TSH showed significantly positive correlations with serum total cholesterol, triglyceride, and low density lipoprotein cholesterol regardless of sex, age, season, obesity, or menopausal status (all p < 0.01). Finally, TSH concentration was positively related to the prevalence of metabolic syndrome. Conclusions: We demonstrated the association between TSH concentration within the normal reference range and serum lipid levels. TSH concentration varies according to sex, age, season, and body mass index (only in males). Moreover, high normal TSH levels were significantly associated with an increased prevalence of metabolic syndrome, which may be of importance when evaluating subjects with high normal TSH concentration.

40세 이하 관상동맥 질환자에서 장기적 임상 관찰

이승욱,정명호,박종철,박형욱,조상희,김원,김계훈,이상현,강경태,조장현,김남호,김건형,안영근,조정관,박종춘,강정채 全南大醫大 2001 전남의대학술지 Vol.37 No.2

老齡者의 建康을 위한 生活習慣 實態調査

申東敏,長鳳愚,李揆文,金賢俊,金昌範,崔宗洙,崔鍾晥,李鍾珏,金圭碩,趙庚旭,金鍾聲,朴鍾振 平生體育硏究所 論文集 1987 平生體育硏究所 論文集 Vol.2 No.-

With a view to examining the habit and method in which old people maintain and improve their health, we consulted 551 men and 444 women who were over 65 years old, and concluded as follows: 1. Habit of eating It's been revealed that most of the old people keep a regular eating hour, control the quantity of meal or eat a little food, and prefer vegetables to meat. 2. Mental health Old people feel the necessity for mental health and most of them (about 80%) keep good mental health without becoming pessimistic. Their chief concenrs have turned out to be about their sons and daughters and their own health and finance. 3. Managing Good Health It has been revelaed that old people do not possess any special kind of secret to keep good health but taking plenty of rest, regular meal, and proper exercise, and many of them (about 60%) have been shown to eat invigorant food that they can easily get around their living area. 4. Physical Functions It has been revealed that many old people are conscious of some symptoms about their parts of body and about to percent of them suffers from neuralgia. Some measures like medical aid especially for old people need to be taken as soon as possible. As we have found that the health-keeping method of old people consists plenty of rest, proper exercise, and appropriate nutrition, we can conclude that the recreatonal and culture facilities and programs for keeping old people in good health should be developed. It is also necessary to study food that contribute to their health and develop ways to get rid of the stresses to which they are exposed in their daily life. Presides, it is natural that government found an administrative department to deal with these matters effectively.

사람 Low Density Lipoprotein (LDL)에 대한 Rooibos tea의 항산화 활성

조경자,김희숙,박종옥,류병호 경성대학교 2001 論文集 Vol.22 No.1

This study was investigated to antioxidative activity of crude catechin extracted from Rooibos tea against low density lipoprotein(LDL) oxidation. The crude catechin inhibited the copper-mediated oxidation of human LDL at the concentrations of 50 and 100 ㎍/㎖ in the presence of 5 μM CuSO4. The crude catechin also inhibited LDL oxidation induced by macrophage J774 cell. LDL oxidized by copper mediated and cell induced oxidation was degraded by macrophage at a much greater rate than native LDL. The electrophoretic mobility of oxidized LDL by addition of crude catechin was faster than that of native LDL. The results provided a possibility that crude catechin of Rooibos tea might protect LDL against oxidation in atheroscleotic lessions.

α1,3-Gal Knock out Pigs Increases N-Glycolylneuraminic Acids

Jong-Yi Park,Mi-Ryung Park,Hong-Thuy Bui,Deug-Nam Kwon,Min-Hui Kang,Mihye Oh,Jae-Woong Han,Ssang-Goo Cho1,Chankyu Park,Hosup Shim,Hye-Min Kim,Man-Jong Kang,Jin-Ki Park,Jeong-Woong Lee,Kyung-Kwang Lee 한국동물번식학회 2012 Reproductive & Developmental Biology(Supplement) Vol.36 No.2s

In this study, we examined whether Hanganutziu-Deicher (H-D) antigens are important as an immunogenic non-a1,3-galactose (Gal) epitope in pigs with a disrupted a1,3- galactosyltransferase gene. The targeting efficiency of the AO blood genotype was achieved (2.2%) in pig fibroblast cells. A total of 1800 somatic cell nuclear transfer (SCNT) embryos were transferred to 10 recipients. One recipient developed to term and naturally delivered two piglets. The a1,3-galactosyltransferase activity in lung, liver, spleen, and testis of heterozygote a1,3-galactosyltransferase gene knockout (GalT-KO) pigs was significantly decreased, whereas brain and heart showed very low decreasing levels of a1,3- galactosyltransferase activity when compared to those of control. Enzyme-linked lectinosorbent assay showed that the heterozygote GalT-KO pig had more sialyla2,6- and sialyla2,3- linked glycan than the control. Furthermore, the heart, liver, and kidney of the heterozygote GalT-KO pig had a higher N-glycolylneuraminic acid (Neu5Gc) content than the control, whereas the lung of the heterozygote GalT-KO pig had Neu5Gc content similar to the control. Collectively, the data strongly indicated that Neu5Gc is a more critical xenoantigen to overcoming the next acute immune rejection in pig to human xenotransplantation.

Amylodextrin과 Acrylonitrile 및 Styrene의 Graft共重合

趙慶來,張貞戴,朴鍾範 新羅大學校 1991 論文集 Vol.31 No.1

Graft copolymerization of acrylonitrile(AN) and styrene(Sty)onto amylodextrin(AD) was carried out using ceric ammonium nitrate(CAN) as initiator under a variety of condition. The graft efficiency of PSty-AD increased by increasing CAN concentration from 0.001mol/1 to 0.0028mol/1, however, at higher CAN concentration(i.e., up to 0.004mol/1.) it decreased. In case of PAN-AD, the efficiency increased with increase in CAN concentration even at higher level. Increasing the AN concentration from 1% to 8% caused a significant enhancement in grafting. The same held true for raising the copolymerization temperature within the range of 25℃-50℃. The graft efficiency of PAN-AD increased by increasing AN concentration, while that of PSty-AD only increased by increasing Sty concentration from 0.5mol/1 to 3.0mol/1. The grafting reaction proceeded initially at a fast rate and decreased with time. The grafted polymers showed increased crystallinity and much lower water solubility as compared with AD. Furthermore the grafted polymers showed no reaction with iodine tincture

분비촉진물질 투여 후 흰쥐 턱밑샘에서 상피성장인자 수용체와 신경성장인자 수용체의변화양상

김은경,김정기,조의식 대한해부학회 1999 Anatomy & Cell Biology Vol.32 No.5

식품가공공장 폐수의 미생물학적 처리 및 응용 : 미생물 균체단백질 회수 Recovery of Mycelial Proteins

조성환,최종덕,이상열,기우경,김재욱 國立統營水産專門大學 附設 水産科學硏究所 1989 수산과학연구소보고 Vol.1 No.-

식품공장 폐수에는 각종 유기물을 다량 함유하고 있다. 이와 같은 식품공장 폐기물의 미생물학적 처리효과를 검토하는 동시에 폐수 중에서 배양된 곰팡이 균체단백질을 분리·회수함으로 해서 폐수처리비용을 절감하고, 단백질 함량이 높은 균체단백사료를 개발할 목적으로 식품공장 폐수의 BOD 및 COD를 낮은 수준으로 감소시키고 증식속도가 빠르며 균체수율이 높고 고농도의 단백질을 함유하며 소화율이 높은 균체를 생산할 수 있는 곰팡이로 Aspergillus fumigatus를 분리·선발하였고, 선발된 균주의 최적배양조건인 35∼40℃, pH 4.0∼4.5에서 pilot plant의 연속배양장치를 이용하여 주정공장 폐수를 기질로 하여 일정시간 동안 배양하여 폐수의 BOD, COD를 90% 이상 감소시켜 폐수정화를 도모할 수 있었으며 균체단백질을 기준 배합사료에 첨가하여 실시한 동물사양 시험 결과, 사양동물의 체중증가량과 단백질 이용율이 대조표준 시험구에 준할 수 있는 효과를 보여 주었다. The rationale for the use of fungi in treating waste streams from food processing plants has been that of incorporating the dissolved and suspending nutrients into a macroscopic organism which can be filtered out readily. In order for a process using fungi to meet these objectives we examined a strain of fungi, Aspergillus fumigatus, which grew well on a variety of polysaccharide-containing materials and showed both efficient BOD removal and high quality protein recovery. In this experiment the fungal choice was based on the laboratory screening studies where the criteria used was BOD and COD reduction, growth response, mycelial yield, and the ability to compete with the natural flora. In the fermentation system used for the continuous culture of Aspergillus fumigatus the best combination of operating variables, inoculum ratio. temperature, initial pH, fermentation time and agitation rate was 5%(v/v), 35∼40℃, pH 4.5∼5.0, 2days and 150rpm, respectively. The fungus reduced BOD and COD to 94.0 and 90.4%, respectively and 3.15g of dry mycelium per liter of alcohol waste was harvested during 48hr of incubation time. The protein efficiency ratios for the control diet and the experimental diet containing the fungal protein were 3.42±0.15 and 3.40±0.43, respectively.