Pretreatment with Lycopene Attenuates Oxidative Stress-Induced Apoptosis in Human Mesenchymal Stem Cells

( Ji Yong Kim ),( Jai Sung Lee ),( Yong Seok Han ),( Jun Hee Lee ),( Inhyu Bae ),( Yeo Min Yoon ),( Sang Mo Kwon ),( Sang Hun Lee ) 한국응용약물학회 2015 Biomolecules & Therapeutics(구 응용약물학회지) Vol.23 No.6

Human mesenchymal stem cells (MSCs) have been used in cell-based therapy to promote revascularization after peripheral or myocardial ischemia. High levels of reactive oxygen species (ROS) are involved in the senescence and apoptosis of MSCs, causing defective neovascularization. Here, we examined the effect of the natural antioxidant lycopene on oxidative stress-induced apoptosis in MSCs. Although H2O2 (200 mM) increased intracellular ROS levels in human MSCs, lycopene (10 μmM) pretreatment suppressed H2O2-induced ROS generation and increased survival. H2O2-induced ROS increased the levels of phosphorylated p38 mitogen activated protein kinase (MAPK), Jun-N-terminal kinase (JNK), ataxia telangiectasia mutated (ATM), and p53, which were inhibited by lycopene pretreatment. Furthermore, lycopene pretreatment decreased the expression of cleaved poly (ADP ribose) polymerase-1 (PARP-1) and caspase-3 and increased the expression of B-cell lymphoma 2 (Bcl-2) and Bcl-2-associated X protein (Bax), which were induced by H2O2 treatment. Moreover, lycopene significantly increased manganese superoxide dismutase (MnSOD) expression and decreased cellular ROS levels via the PI3K-Akt pathway. Our findings show that lycopene pretreatment prevents ischemic injury by suppressing apoptosis-associated signal pathway and enhancing anti-oxidant protein, suggesting that lycopene could be developed as a beneficial broad-spectrum agent for the successful MSC transplantation in ischemic diseases.

Pretreatment with Lycopene Attenuates Oxidative Stress-Induced Apoptosis in Human Mesenchymal Stem Cells

Kim, Ji Yong,Lee, Jai-Sung,Han, Yong-Seok,Lee, Jun Hee,Bae, Inhyu,Yoon, Yeo Min,Kwon, Sang Mo,Lee, Sang Hun The Korean Society of Applied Pharmacology 2015 Biomolecules & Therapeutics(구 응용약물학회지) Vol.23 No.6

Human mesenchymal stem cells (MSCs) have been used in cell-based therapy to promote revascularization after peripheral or myocardial ischemia. High levels of reactive oxygen species (ROS) are involved in the senescence and apoptosis of MSCs, causing defective neovascularization. Here, we examined the effect of the natural antioxidant lycopene on oxidative stress-induced apoptosis in MSCs. Although $H_2O_2$ ($200{\mu}M$) increased intracellular ROS levels in human MSCs, lycopene ($10{\mu}M$) pretreatment suppressed $H_2O_2$-induced ROS generation and increased survival. $H_2O_2$-induced ROS increased the levels of phosphorylated p38 mitogen activated protein kinase (MAPK), Jun-N-terminal kinase (JNK), ataxia telangiectasia mutated (ATM), and p53, which were inhibited by lycopene pretreatment. Furthermore, lycopene pretreatment decreased the expression of cleaved poly (ADP ribose) polymerase-1 (PARP-1) and caspase-3 and increased the expression of B-cell lymphoma 2 (Bcl-2) and Bcl-2-associated X protein (Bax), which were induced by $H_2O_2$ treatment. Moreover, lycopene significantly increased manganese superoxide dismutase (MnSOD) expression and decreased cellular ROS levels via the PI3K-Akt pathway. Our findings show that lycopene pretreatment prevents ischemic injury by suppressing apoptosis-associated signal pathway and enhancing anti-oxidant protein, suggesting that lycopene could be developed as a beneficial broad-spectrum agent for the successful MSC transplantation in ischemic diseases.

급성하벽심근경색에서 전흉부유도 ST절 하강의 의의

정병천,배호상,곽동훈,김영태,배용학,황종현,임현주,류재근,조용근,채성철,전재은,박의현 慶北大學校 醫科大學 1997 慶北醫大誌 Vol.38 No.2

목적 : 급성하벽심근경색증 환자에서 심전도상 흔히 동반되는 전흉부유도 ST절 하강의 의의를 알아보았다. 방법 : 심근경색후 24시간에서 48시간에 얻은 심전도를 기준으로 52명의 급성하벽심근경색증 환자를 전흉부유도에서 ST절의 하강이 있는 군과 없는 군으로 나누었으며 심도자, 관상동맥조영술, 심초음파도 및 방사성 핵종을 이용한 좌심구혈율 측정 등을 시행하였다. 결과 : 1) 좌심실확장기말압은 전흉부유도에서 ST절의 하강이 있는 경우에서 16.9±5.33mmHg로 없는 경우의 11.0±4.02mmHg보다 유의하게 높았다(p<0.05). 그러나 운동량 및 좌심구혈을은 흥부 유도상 ST절의 하강이 있는 경우 7.6±3.0 METs 및 53.9±9.1%로 없는 경우의 9.1±2.7 METs 및 54.6±9.5%보다 낮았으나 통계적 유의성은 없었다(p=0.180, p=0.823). 2) 관상동맥조영술상 전흉부 ST절의 하강이 동반된 군에서는 유의한 좌전하행지나 좌회선지의 협착이 있는 경우가 60.9% 및 57.9%로 ST절의 하강이 없는 군에서의 34.5% 및 7.7%와 비교해 좌관상동맥에 협착이 동반된 경우가 많았다(p=0.050, p=0.001). 3) 관상동맥조영술상 세 혈관질환의 빈도는 흉부유도상 ST절의 하강을 보인 23예에서 10예로 ST절 하강이 없었던 29예중 2예에 비하여 유의하게 많았고(P=0.007), 단 혈관질환의 빈도는 ST절의 하강을 보인 군에서 23예중 5예로 ST절 하강이 없었던 29예중 17예에 비하여 유의하게 적었다(P=0.002). 두 혈관질환의 경우에는 각각 8예와 10예로 양군간에 유의한 차이가 없었다. 4) 흉부유도상 ST절 하강의 빈도는 단 혈관질환 22예 중 5예에서, 두 혈관질환 18예중 8예, 세 혈관질환 12예 중 10예에서 관찰되어 협착이 있는 관상동맥의 수가 많을수록 ST절의 하강이 나타나는 빈도가 많음을 보였다(p=0.00308). 그러나 흉부유도상 나타난 ST절의 하강을 모두 합하여 구한 ST절 하강의 합은 협착이 있는 관상동맥의 수와 통계적인 유의성이 없었다. 5) 심초음파도상 좌심실 하벽의 벽운동은 ST절 하강을 보인 군에서는 akinesia가 83.3%, hypokinesia가 11.1%, 정상 벽운동이 5.6%로, ST절 하강이 없었던 군에서의 dyskinesia가 3.6%, akinesia가 39.3%, hypokinesia가 25.0%, 정상 벽운동이 32.1%와 비교하여 ST절의 하강이 있는 군에서 유의하게 벽운동의 장애가 빈번함을 보였다(p=0.03891). 그러나 좌심실전벽, 측벽 및 중격부위에서는 유의한 벽운동의 차이가 없었다. 결론 : 급성하벽심근경색증 환자의 전흉부유도에서 ST절의 하강이 동반되는 경우 다혈관 질환의 가능성이 높으며 좌심실확장기말압이 상승되었고 운동능력은 감소되어 있었다. 또한 심초음파도상 좌심실하벽의 벽운동장애도 심하였다. 따라서 전흉부 ST절의 하강을 동반한 급성하벽심근경색증 환자는 전흉부유도에 ST절 하강이 없는 경우보다 예후가 불량할 것으로 생각되며, 보다 적극적인 치료가 필요할 것으로 생각된다. The purpose of this study was to determine the significance of precordial ST segment depression appearing during acute inferior myocardial infarction and to assess the correlation of that with angiographic finding. 52 patients were allocated into two groups based on the existence of precordial ST segment depression : 23 patients with ST segment depression and 29 patients without ST segment depression. The extent of coronary artery disease as well as the prevalence of significant stenosis (≥70%) in left anterior descending artery were more frequent in patients showing precordial ST segment depression than in patients not showing them (p=0.050) and the result were similar in cases of left circumplex artery (p=0.001). On echocrdiographic examination of left ventricular (LV) wall motion, it showed more severely decreased inferior LV wall motion in patients showing precordial ST segment depression than in patients not showing them (p=0.03891) even though the other segment of LV wall motion didn't show the statistical difference between both groups. The other cardiac parameters such as LVEDP, exercise amount (METs) and ejection fraction were tend to showing bad profile in patients with precordial ST segment depression. From the above results, we could deduce that patients with precordial ST segment depression have relatively large infarction or concomitant left coronary artery disease, so they need more caution and intensive theraphy.

소아 재발성 호흡기 감염증에서 Broncho-Vaxom�의 임상효과

배종우,차성호,조병수,정사준,최용묵,안창일 한국의학사 1990 診斷과治療 Vol.10 No.9

공조시스템을 적용한 석실 고분내 온ㆍ습도 분포

전용두,이금배,박진양,고석보,전희호 公州大學校 工學硏究院 生産技術硏究所 2005 論文集 Vol.13 No.-

Although the importance of good conservation of historic sites including ancient royal tombs is well aware, still not much attention has been paid for the facilities and systems to preserve those historic sites, which includes precious artifacts as wall paints and carved works, etc. Even the general understanding about the environment of the underground space of tombs is not satisfactory. In Korea, researchers have recently begun addressing the above issue and are making efforts to develop suitable HVAC(heating, ventilating and air-conditioning) systems for conservation of tombs. An experimental investigation was carried out to study the behavior of the HVAC system installed for a tomb. This tomb resembles in size and shape the original stone built tomb of Baek-je Dynasty, which dates back to 6th Century in southwest part of Korean peninsula. In this study, an HVAC system for a tomb(D×W×H=1.3m×3.0m×1.2m) was installed to maintain sui표 indoor conditions for conservation of tomb. The temperature and humidity inside the tomb were measured different of the setting. Finally, performance of the HVAC system is presented and discussed.

재식한 흰쥐 구치 치수조직의 재혈관화와 CGRP 면역반응 신경섬유의 변화

배용철,이승봉,김영진,남순현,김현정 大韓小兒齒科學會 1999 大韓小兒齒科學會誌 Vol.26 No.4

치아 재식 후 시간경과에 따른 치수조직의 재혈관화와 신경재지배의 변화를 관찰하기위해 생후 35일된 흰쥐(Sprague dawley) 56마리를 대상으로 상악우측 제1대구치를 발치 즉시 재식한 후 1일, 2일, 3일, 1주, 2주, 4주, 6주 간격으로 희생시켜 먹물을 이용한 혈관조영술과 CGRP를 이용한 면역조직화학적방법으로 각기 시행하여 다음과 같은 결론을 얻었다. 1. 재혈관화와 CGRP 면역반응신경섬유의 재지배는 재식후 1일에서 근심측 치수조직에서는 이미 이루어졌으나 원심측은 미약했다. 재식후 2일에 전치수조직에서 재혈관화와 신경재지배가 이루어졌으며 이후 6주까지 시간경과에 따라 분포밀도가 증가하였으나 대조군에 비해서는 약하게 나타났다. 2. 재식후 1주에서부터 술후 상아질이 형성되기 시작하였으며 재식후 6주에서는 대조군에 비해 상대적으로 치수강이 좁아진 양상을 보였다. 3. 재혈관화와 신경재지배는 거의 같은 시기에 이루어지는 것으로 나타났으며 밀접한 관련성이 있는 것으로 보인다. The purpose of this study was to investigate the revascularization and reinnervation of calcitonin gene-related peptide immunoreactive nerves in immediately replanted rat molars. First maxillary right molars in 56 rats(35days old) were extracted and immediately replanted. The rats were killed 1, 2, 3, 7, 14, 28 and 42 days after replantation and revascularization of pulpal blood vessels were examined microangiogram with korean traditional ink and reinnervation of pulpal nerve were examined immunohistochemical method using calcitonin gene-related peptide(CGRP) antiserum. The results were as follows; 1. Revascularization and reinnervation of CGRP immunoreactive nerve fibers were observed mesial side whole pulp tissue of replanted teeth. Revascularization and reinnervation of CGRP immunoreactive fiber were made at 2days after replantation in entire pulp of replanted teeth and the distribution density of blood vessels were gradually increased according time elapsed, but did not achieve the density of control. 2. Postoperative dentin formation in replanted teeth revealed at 1 week after replantaton and gradually increased according to time elapsed. 3. Revascularization and Reinnervation of CGRP immunoreactive nerve fibers were established at the same time and it seems to be closed relatationship between revascularization and reinnervation.

패혈증을 동반한 Clostridium perfringens에 의한 간농양 1례

배인규,조용균,홍성수,김승규,조윤행,정두련,김미나,우준희,배직현,류지소 대한화학요법학회 1999 대한화학요법학회지 Vol.17 No.3

저자들은 아직 국내에 보고된 적이 없는 C. perfringens에 관한 간농양과 이에 동반된 전격성 패혈증으로 증상 발현 후 24시간 이내에 사망하였던 증례를 경험하였기에 보고하는 바이다. Clostridial infection usually occurred in association with trauma, malignancy, or intra-abdominal disease. Liver abscess by Clostridium perfringens is very rare and has not been reported in Korea .We experienced a case of liver abscess by C. perfringens in a 70-year-old man who was presented with fever. He developed a progressive jaundice and sepsis. Abdominal CT scan showed about 7×7 ㎝ sized liver abscess and gas formation within abscess. We underwent percutaneous drainage of a hepatic abscess and empirical antimicrobial therapy. The patient died less than 24 hours after presentation. C. perfnngens was identified from blood and aspirate of liver abscess.

CO₂고정을 위한 화학독립영양미생물 Aeromonas sp. strain JS-1의 분리 및 특성

배상옥,곽경오,이용운,김성준,정선용 전남대학교 촉매연구소 2000 觸媒硏究 論文集 Vol.21 No.-

The increase of CO2 in the atmosphere by fossil-fuel combustion has been recognized as a major cause of global warming. Thus many studies on CO2 removal from the combustion gas have been performed, and recently the techniques for biological CO2 fixation by photoautotrophs have also been developed, In this study, extensive screening was conducted to obtain micoorganism with high capability in fixing CO2, and its characteristics was investigated. As a result, a chemoautotrophs identified as a Aeromonas sp. strain JS-1 was isolated from the fresh water. The isolated strain used the molecular hydrogen and CO2 as an energy and a carbon source, respectively. Growth characteristics, which will be used in the development of a continuous culture system related in CO2 fixation, were examined in batch cultivation under conditions of various CO2 and NaCl concentrations, temperature, and pH. The results of the examination showed that the isolated strain had a good growth to high CO2 concentration(40%), indicating applicable in removing CO2 discharged from industries.

동절기 신관리 석실고분 내부의 온·습도 분포특성

전용두,이금배,김홍기,고석보 공주대학교 문화재보존과학연구소 2004 문화재과학기술 Vol.3 No.1

개방된 고분의 장기보존은 조상의 얼이 담긴 문화재를 길이 후손에 물려주어야 하는 우리들의 시대적 사명이라 할 수 있다. 그런데 고분 내부의 공기조화를 필요로 하는 경우 설비측면에서 고분내부의 공기조화 문제를 검토한 사례는 그 동안 많지 않았다. 특히 고분 내부환경에 관한 온·습도 자료는 매우 제한적으로 알려져 있는 상태이다. 본 연구는 고분의 공기조화와 관련하여 부하산정 등의 기초자료로 활용하기 위한 고분내부의 온·습도자료를 원격측정 기술을 적용하여 획득한 결과를 소개하며, 이를 통하여 고분내부의 실내환경 조건에 대한 일반의 이해를 돕고, 더 나아가 공조기 설치에 필요한 기초자료를 제공하고자 수행하였다. 측정 연구대상은 충남 공주시 신관동에 소재한 신관리석실고분(충청남도 기념물 제 7호)으로서 백제후기의 횡혈식 석실고분으로서 현실의 크기는 폭 1.25m, 깊이 2.73m 그리고 높이 1.50m이며, 현실입구는 개방된 상태에서 실내 5채널 및 실외공기에 대하여 각각 온도와 습도를 측정하였으며, 본 본문에서는 동절기(12월부터 다음 해 3월까지)의 자료를 소개한다. In recent years the importance of the preservation of cultural artefacts like ancient tombs has been widely accepted domestically and internationally as the quality of life improves. However not much attention has been paid for the facilities and systems to preserve those artefacts. Even the general understanding of the preservatory environment of the underground space as tombs is poor. In the present study, the temperature and relative humidity inside a selected artefact, Shinkwan-ri tomb, are monitored to improve the understanding of the indoor thermal environment, which may possibly be used when one consider air-conditioning for preservation. Total of 12 channel data are collected by using remote data acquisition system based on the cdma(Code Division Multiple Access) technology. Three-month-data representing winter season is reported with discussions.

기관지천식 환아에서 Ketotifen(Zaditen^(�))의 임상적 효과에 관한 연구

배종우,차성호,조병수,정사준,최용묵,안창일 한국의학사 1988 診斷과治療 Vol.8 No.4