근원섬유단백질의 생화학적 특성의 연령에 따른 변화

양융,채한주,홍상필,신동범,신완철 한국노화학회 1992 한국노화학회지 Vol.2 No.1

카라기난 분해효소 생산균의 분리, 동정, 및 효소생산 최적 조건

양승택,주동식,박중제,이정석,김명식,이응호 한국미생물생명공학회 ( 구 한국산업미생물학회 ) 1996 한국미생물·생명공학회지 Vol.24 No.6

해양 동식물, 토양 등으로부터 분리한 카라기난 분해능이 확인되었던 80여 균중에서 환원당 생성능이 가장 높았던 strain no.43 균주를 최종 시험균주로 선택하여 이 균주의 최적 효소 생산 조건을 실험하였다. 질소원으로는 nutrient broth 0.7% 농도가 가장 적절한 조건이었고, 탄소원의 농도는 카라기난 0.2%가 가장 높은 효소활성을 나타내었다. NaCl 농도는 1.5%, pH, 온도는 각각 7.0, 30℃로 이 조건에서 96시간 배양하는 것이 가장 적절한 효소 생산 조건이었음이 확인되었다. 각종 생화학 실험을 통해 동정을 행한 결과 그람음성 간균으로 oxidase, catalase 생성의 호기성균, indole 비생성, proline, arginine, serine, citrate, lactate 이용성, gelatin 액화, glucose, fructose, maltose 비이용, mannitol 이용 등의 특성으로부터 Pseudomonas alcaligenes로 동정할 수 있었고 Pseudomonas alcaligenes JCL-43으로 명명하였다. The 80 strains which produce carrageenan degrading enzyme were isolated from soils, mud, seaweed, marine moluscus and echonodermata samples. Among them, one isolated strain, which showed the highest activity to produced carrageenan degrading enzyme, was used for this study. The isolated strain was identified as Pseudomonas alcaligenes through its morphological, biochemical, and physiological characteristics. The best conditions for enzyme production were 0.7% nutrient broth and 0.2% carrageenan as nitrogen and carbon source, respectively. The optimal pH, NaCl, temperature and culture time for carrageenan degrading enzyme were 7.0, 1.5%, 30* and 96hrs, respectively.

영아 천식의 임상적 고찰 : Asthma prospective score에 의한 Based on Asthma Prospective Score

양은석,노영일,문경래,박영봉,박상기,변주남 조선대학교 부설 의학연구소 1999 The Medical Journal of Chosun University Vol.24 No.1

A Clinical study was made on 40 patients for infantile asthma less than 2 years old who had been admitted to the Department of Pediatrics of Chosun University Hospital from January 1997 to December 1998. We evaluated family history of allergic diseases, clinical features, serum IgE level, eosinophilia, chest radiologic findings and response to bronchodilator. The results obtained were as follows ; 1) The male to female ratio of patients studied was 4 : 1. 2) Eighteen of the 40 patients (45.0%) had a family history of allergic diseases, of which 10 patients had maternal allergic diseases. 3) Atopic dermatitis was the most common disease (37.5%) among the associated allergic diseases. 4) Thirty two of the 40 patients (80.0%) showed high serum total 19E levels. 5) Only 5 of the 20 patients who were examined by MAST showed positive reaction. 6) The frequency of chest radiologic findings observed in these patients was as follows ; hyperinflation (52.5%), increased bronchovascular marking(30.0%), no pathologic findings(25.0%), and pneumonic infiltration(25.0%). 7) In response to bronchodilator 28 cases(70.0%) were effective. 8) When we divided the 40 patients into two groups by the diagnostic criteria of Asthma prospective score (AP score), 30 patients(75.0%) were included in a compatible group. On the basis of AP score, family history of major or minor allergic dieases and the responsiveness to bronchodilator were important factors for the diagnosis of asthma.

고양이 위 평활근에서 KCI에 의한 근수축에 대한 Protein Kinase의 조절

심상수,김승록,심혜선,이덕주,한상준,윤신희,조양혁,김명석 大韓消化器病學會 1998 大韓消化器病學會誌 Vol.32 No.3

성인 막성신병증의 임상적 고찰

양종오,이상주,박기현,장윤경,이강욱,서광선,신영태 충남대학교 의학연구소 2001 충남의대잡지 Vol.28 No.2

Membranous nephropathy (MN) is a glomerular disease characterized by diffuse thickening of the glomerular basement membrane without significant mesangial proliferative change and is usually manifested by the nephrotic syndrome. To evaluate the clinical and pathologic characteristics of this disease in adults, we analyzed 65 patients with primary membranous nephropathy who were diagnosed at Department of Internal Medicine, Chungnam National University Hospital from February 1986 to February 2001. The results are as follows: 1) Of total 96 patients with membranous nephropathy, 67.7% was primary MN. Hepatitis B-associated MN and lupus MN were 22.9%, and 9.4%, respectively. Median duration of follow-up was 35.1 months(0.3∼177) in primary MN, 49.6 months(2.5∼103) in lupus MN, and 35.6 months(0.5∼108) in hepatitis B-associated MN. 2) Mean age of the patients with primary MN at the time of diagnosis(43.3 years) was significantly higher than that of lupus MN(30.4 years) and HBV-associated MN(36.2 years). Male to female ratio of primary MN was 1.03:l. 3) At the time of diagnosis, 89.2% of primary MN was presented with nephrotic syndrome. Twenty seven percent of patients showed hypertension. Hematuria was found in 58.5% of primary MN patients. Azotemia was noted in 3.1 %. 4) Global sclerosis, tubular atrophy and interstitial fibrosis were noted in 39.8, 16.1 and 11.3% of primaty MN, respectively. IgG and C3 were deposited on the glomerular capillary loop in 92.7% and 29% of primary MN patients. There was no significant correlation between the electron microscopic pathologic stage and clinical findings. 5) Of 56 patients with primary MN followed more than 6 months, 50(90.9%) patients were treated with prednisolone, cyclophosphamide or cyclosporin. Complete remission was obtained in 29 patients(51.8%), partial remission in 6 patients(10.7%), and no response in 18 patients(32.1%). Of 6 patients who were not treated with immunosuppressive drugs, 3 patients showed spontaneous complete or partial remission. Three patients who did not show clinical improvement progressed to end-stage renal failure. There were no significant clinical difference including renal pathology at the time of diagnosis between the patients who showed complete or partial remission and patients who did not. In conclusion, it was difficult to predict the long-term prognosis of primary MN at the time of diagnosis, clinically. The response to therapeutic modality would be the most important to predict long-term prognosis of primary MN.

고양이 위 평활근 수축에 대한 Vanadate와 Pervanadate의 효과

심상수,최효봉,심혜선,이덕주,윤신희,한상준,김명석,조양혁 大韓消化器病學會 1999 大韓消化器病學會誌 Vol.33 No.1

본태성 고혈압이 동반된 당뇨병환자의 혈중 인슐린 농도

양동호,홍세용,성기범,안무영,윤신구,박형국,양광익,황주호,신현길 순천향의학연구소 1997 Journal of Soonchunhyang Medical Science Vol.3 No.1

Multiple lines of evidence link elevated blood pressure with diabetes mellitus. Specifically, it has been proposed that resistance to insulin-stimulated glucose uptake and hyperinsulinemia may play a central role in the cause and clinical course of hypertension. In diabetes mellitus, insulin resistance and/or hyperinsulinemia is an common finding and it is interesting whether the hyperinsulinemia may play a signigicant role in hypertension with DM in the same way as in essential hypertension without diabetes mellitus. The object of this study was to compare insulin and c-peptide levels between age, sex, and obesity matched two groups(DM with DM without hypertension). Method The study group consisted of 55 male diabetes mellitus patients, aged between 45-55 (years). Patients with obesity (body mass index · 30 ㎏/m2), renal disease (proteinuria · 300 ㎎/24hr urine), and secondary hypertension were excluded. Insulin and c-peptide were measured in overnight fasting state and after oral administration of glucose(75 gm). In the fasting, venous plasma glucose levels were similar in the hypertensive and control group(132 ±7 ㎎/dl vs 135 ±8 ㎎/dl). In the fasting, venous plasma insulin levels were higher in the hypertensive than in the control group (10.9 ±5.3 μIU/ml vs 5.5 ±3.9 μIU/ml, p = 0.0001). After loading with 75 gm glucose, venous plasma insulin level seems to be higher in the hypertensive patients than in the control patients, but the difference was not signigicant statistically (27.2 ±17.5 μIU/ml vs 19.9 ±18.9 μIU/ml, p = 0.1297). The mean insulin concentration of the essential hypertensive patients with diabetes mellitus was twice that of the normotensive patients with diabetes mellitus. In control group, there was a direct relations between insulin level and c-peptide, in both fasting state (R = 0.617, p = 0.0001) and glucose-loaded state (R = 0.531, p = 0.001). But in hypertensive group, there was no relations between insulin level and c-peptide, in both fasting state (R = 0.257, p = 0.2738) and glucose-loaded state (R = 0.307, p = 0.1885).

Development of a Chest‑Belt‑Type Biosignal‑Monitoring Wearable Platform System

Joo‑Hyeon Lee,Hyun‑Seung Cho,Jin‑Hee Yang,Sang‑Min Kim,Jeong‑Whan Lee,Hwi‑Kuen Kwak,Je‑Wook Chae 대한전기학회 2020 Journal of Electrical Engineering & Technology Vol.15 No.4

The purpose of this study was to develop a wearable platform system that can detect and acquire a soldier’s biosignals (i.e., heart activity signal, respiration rate, etc.) in a nonrestrained, unconscious manner. These detected biosignals are transmitted to a processing device to analyze and monitor the soldier’s physical status. To achieve this, textile-based heart activity electrodes and a strain gauge sensor for the respiration signal measurement were developed, and their performances in detecting each signal were verifed. These sensors were embedded in a chest belt to design a wearable platform that can simultaneously measure heart activity and respiration signals. The sensor part of the chest belt has a dual layer structure to detect high-quality signals. Stretch fabric was used on the outer layer and a respiration sensor was attached to the belt. On the inside layer, a non-stretch fabric was used as the base fabric and a heart activity-sensing electrode, that is capable of taking measurements using a modifed lead-II heart activity signal induction method, was embroidered onto the fabric. Subjects were asked to wear the chest belt, and a biosignal processor module was attached to verify the system’s performance while simultaneously acquiring the heart activity and respiration signals. More specifcally, it was confrmed that the two signals were detected in a stable. It is expected that the biosignal-monitoring wearable platform system developed in this study will be able to efectively analyze and monitor soldiers’ biosignals.

제1형 당뇨병 환자에서 췌도세포 동종이식의 반복시행

양태영,정인경,서인아,오은영,조건영,오승훈,김성주,정재훈,민용기,이명식,이문규,김광원,도영수,주성욱 대한당뇨병학회 2002 Diabetes and Metabolism Journal Vol.24 No.4

연구배경:췌도세포 이식은 시술이 간편하고 안전하기 때문에 반복이식이 가능하여 그 동안 여러 센터에서 반복이식의 성공을 보고한 바 있다. 국내에서도 본 병원을 비롯하여 몇몇 센터에서 췌도세포 이식이 활발히 시도되고 있으나 사람에서의 동종이식 및 동종 이식의 반복시행은 보고된 바 없는 실정이다. 저자 등은 국내 최초로 췌도세포 동종이식을 시행하여, 혈청 C­펩타이드가 증가하고 인슐린 요구량이 감소하였으며, 혈당농도와 당화혈색소의 안정을 보였다. 그러나 이식 70일 후 다시 인슐린 요구량이 증가하고 C­펩타이드가 감소하여 췌도세포 이식을 다시 시행한 바 있다. 2차 이식 후 70여일이 지난 현재 다시 혈청 C­펩타이드가 증가하고, 인슐린 요구량이 더 감소되어 췌도세포 이식에서 반복 시행의 유용성을 보고하는 바이다. 방법:환자는 32세 남자로 17년전 당뇨병 진단 후 인슐린 치료 중이었으며, 3년전 부터는 만성신부전증으로 혈액툭석을 하고있었다. 1999년 12월 25일 신장이식을 하였고 3일 후 췌도이식을 하였으며, 두번째 이식은 70일 후 시행하였다. 췌도분리는 변형된 Recordi방법과 비연속성 자당 농도차(discontinuous density gradient)를 이용하였으며 분리한 췌도는 배양 후 환자의 신장기능이 정상화되고 미생물학 검사에서 음성을 확인한 후 경피경간으로 간문맥을 접근하여 16G 폴리 에틸렌 카테터를 이용하여 간실질에 이식하였다. 결과:1차 췌도이식시 순수분리전 췌도수는 210,000개, 순수분리후 획득한 췌도수는 90,000개, 순도 95%, 세포양 1.0mL 이었고, 2차 췌도이식시 순수분리전 췌도수는 420,000개, 순수분리후 획득한 췌도수는 370,000개, 순도 95%, 세포양 1.5mL 이었다. 1,2차 모두 간문맥을 통해 약 20분에 걸쳐 간실질에 주입하였다. 췌도이식전 인슐린요구량은 75∼75U/일, HbA1e 8∼10%, C­펩타이드 0.6ng/mL 였으며, 1차 췌도이식수 7일째 인슐린 요구량은 40U/일, C­펩타이드 1.5ng/mL, FPS 109mg/mL 였고, 40일 추적관찰 후 인슐린 요구량이 36U/일, C­펩타이드 1.8ng/mL, HbA1e 6.5∼7.0%로 안정되었다. 그러나 이식 50일째부터 인슐린 요구량이 50∼56U/일, C­펩타이드 0.6ng/mL, FPS 130∼200mg/dL로 혈당 조절이 불안정하여 다시 췌도이식을 시행하였다. 2차 이식후 50일이 경과한 현재, 인슐린 요구량은 26U/일, C­펩타이드 1.8ng/mL, FPS 90∼120mg/dL로 다시 안정되었다. 결론:췌도이식은 반복이식이 가능하며, 본 환자의 경우 인슐린요구량 감소, 혈당의 안정화 및 C­펩타이드가 상승하여 이식한 췌도의 기능을 확인할 수 있었고, 향후 스테로이드 등 면역억제가 유지 용량으로 감량되면 인슐린 요구량은 더 감소될 것으로 기대된다. Over the past 20 years, significant advances have been made in human islet transplantation. However, cases of prolonged insulin independence after islet allotransplantation have rarely been reported and over time, a slight, gradual decrease in insulin secretion appears to occur, as suggested by the lower C-peptide. Although preliminary clinical success achieved over the past few years has been considerably higher with whole pancreatic transplant than with isolated islet grafts, both approaches remain experimental. Islet grafts might gain, over time, increasing credibility and might eventually provide an easier alternative in terms of grafting procedures and patient management, as compared with the more "traumatizing" whole-pancreas transplantation. Also, using islet, re-transplantation is possible. But it is not known whether re-transplantation of islet could be suitable for those patients who lost grafted islet function. The aim of the present study was to investigate the benefits of re-transplantation of islet in previously simultaneous islets-kidney transplant (SIK) patient who have lost graft function. Methods : The recipient was a 32 year old male. First islet transplantation was underwent at December 25, 1999. However, the grafted islets lost function after 70 days. So we performed re-transplantation of islets. The isolation of islet was conducted sterilely on a laminarflow hood and isolated by a modified Recordimethod. The islet was injected slowly into the liver via a cannular placed in the potalvein for 20 minutes. Results : Transplanted islets were 90,000 IEq at first islet transplantation, 370,000 IEq at second islet transplantation. The insulin requirement was reduced from 75-85 to 35-40 U/day, the basal C-peptide level was 1.5 ng/mL at 7 days posttransplant Unfortunately, the grafted islets lost function after 70 days. After second transplantation, the insulin requirement was reduced to 26 U/day. Conclusions : Despite the continuous need for exogenous insulin therapy, islet transplantation can prevent wide glucose fluctuations, thus resulting in normalization of glycemic control and improvement in HbAlc, and also, show that islets can be successfully and safely re-transplanted intraportally in patients who have lost previously grafted islet function (J Kor Diabetes Asso 457~466, 2000).

産卵鷄에 대한 海藻粉의 飼料的 價値에 관한 硏究

梁昇柱 건국대학교 1977 論文集 Vol.6 No.1

This experiment was conducted to determine the feeding value of kelp meal collected from Daejung, Cheju Island for laying hens. A total of 240 births of 28 week-old babcock hen was alloted to 4 treatments, 4 replicates per treatment, according to completely randomized design to find out the effects of substitution levels (0, 5, 10 and 15%) of kelp meal for wheat bran on the laying performance of experimental thirds for 70 days. The results obtained are summarized as follows : 1.Kelp meal studied were analyzed for the contents of proximate general composition (moisture 13.73%, crude protein 9.66%, crude fat 1.81%, crude fiber 9.08%, crude ash 21.96%, nitrogen free extracts 43.86%). 2.Egg-laying rates for C (control allot) and T1 (5% allot) were higher than those of T2 (10% allot) and T3 (16% allot), but no statistical significant difference was found among all treatments. 3.In feed intake, the statistical significant difference (p < 0.06) was observed among all treatments, and the amounts of feed take for T1 was higher than mothers, in the other hand those for T3 was lower than ethers. The results of feed conversion was the some with that of feed intake. 4.The average egg weight for T3 was higher than ethers, but there was no statistical significant difference among all treatments. The weight of egg shelf was showed the some results with egg weight. Egg folk colour for T2 and T3 was more dark than C and T1. 5.In the nutrient utilizability, no statistical significant difference was found among treatments, but the crude fiber utilizability was showed the significant difference among treatments. Especially thc result of T2 was lower than ethers. 6.Based on these results described above, it boomed that the substitution of wheat bran with kelp meal up to 5% level could be uses without adverse effect in the ration for laying hens.