CAI를 활용한 DIY 목공조립 프로그램이 정신지체학생의 눈·손·발 협응능력에 미치는 효과

신동범 한국특수교육총연합회 2004 현장특수교육연구보고서 Vol.- No.-

미생물 포괄고정화를 위한 고정화재의 비교검토

신동범,윤창훈,강경수 제주대학교 환경연구소 1997 환경연구논문집 : 제주대 Vol.5 No.-

We conducted the experiments to find out the appropriate gelling materials for the treatment of waste water by immobilizing microorganisms inside the gels. The gelling materials known to immobilize microorganisms or cells in them are polyacrylamide, polyvinylalcohol, sodium alginate, κ-carrageenan and chitosan. The gel strengths were determined by the breaking points of the pellet using a rheometer with reaction time. The polyacrylamide gels were retained their solidities and activities excellently compared with the other pellets of polyvinylalcohol, sodium alginate and chitosan with reaction time. It is considered that, in the other hand, the polyacrylamide gels are toxic to the single microorganisms and cells by free radicals during the formation of gels. We used activated sludge for the mmobilization, so it didn't matter the toxicity caused by free radicals. The profiles of waste water treatment by all the immobilized pellets using in this study were showed nearly similar patterns, but gel strength of all the gels except polyacrylamide pellets were getting weak and weak as reaction time goes by. It was concluded that the polyacrylamide gels are excellent for immobilization of activated sludge for waste water treatment, but in the case of immobilization of single microorganisms or cells, natural polysaccharides of sodium alginate, κ -carrageenan or chitosan are thought to be suitable, and it must be developed the techniques for elevating gel strength of nautral polysaccharides.

DNA에 관한 메일라드 노화이론

신동범,이선주 제주대학교 생명과학기술혁신센터 2002 제주생명과학연구 Vol.5 No.-

The Maillard reaction theory of aging have been proposed in 1980s, hypothesizing that chronic formation and accumulation of Maillard products in biomolecules was a determining factor of the rate of aging of species. Proteins with long life spans serve as the primary targets of exposure to chemical demage, which are detectable n the form of advanced glycation end products(AGEs) and advanced lipoxidation end products(ALEs) as well as modified oxidative reaction by reactive oxygen species are an important factor of age-related damage. The Mallard reaction of DNA also occur in important living organisms. Although measurement of AGEs and ALEs in proteins are useful for determining the rate and extent of Maillard damage, but there are no chemical biomarkers of Maillard damage to DNA. The extent of DNA damage represents a balance between the rate of modification and the rate and fidelity of repair. Damage to DNA accumulates not in the form of modified nucleic acids, but some errors in repair that affect the expression and structure of proteins. Although these mutations are not detectable in DNA by conventional analytical techniques, purines and pyrimidines modified by Maillard reaction intermediates may be detectable in urine, and studies on these compounds should provide insight into the role of Maillard reactions of DNA in aging and disease.

발병률 점점 높아지는 경향 - 약제선택시 내성균 문제 고려해야

신동범 한국작물보호협회 1994 자연과 농업 Vol.15 No.2

유전자조작 농산물의 개발현황과 그 안전성 평가

신동범 제주대학교 환경연구소 1998 환경연구논문집 : 제주대 Vol.6 No.-

In recent years, the safety of foods and food ingredients from genetically modified plant varieties has been widely discussed. Genetical modification technique to transfer genetic information from one species to another has been possible for about two decades. The ability to transform, or insert new DNA into the plant cell, exists now for most crops, including maize, wheat, soybean, cotton, and rice. Early products of this technology include plants with enhanced insect and disease resistance, and improved herbicide tolerance. Future goals are to increase higher yield, improve nutritional and processing characteristics, and produce specialty oils, carbohydrates, and proteins. Some examples of genetically modified products that have been commercialized are FLAVR SAVR™ tomato, Bollgard^㉿ cotton, Round-Up Ready㉿ soybean, Maximizer™ and NatureGard™ corn, Laurical㉿ Canola oil, and Feedom IF" squash. The international agencies. OECD and WHO, and USDA. FDA and EPA in United States issued a policy statement on foods derived from new plants varieties each other. The document from FDA discussed criteria that should be considered by plant breeders and food manufacturers in assessing the safety of foods derived from new plant varieties before they enter the marketplace. Even though genetically modified plants and their foods are now evaluated safe enough by scientific experiments. the present scientific knowledges won't the truth forever. The ultimate judges of new products of biokhnology will be consumers. They will be more confident in the safety of foods derived from plants deveolped by new technology if scientist and developers share information on safety testmg.

메일라드 반응과 노화

신동범 제주대학교 생명과학연구소 1999 제주생명과학연구 Vol.2 No.-

It is obvious that the Maillard reaction, also called glycation or non-enzymatic glycosylation of proteins by carbohydrates, is related with molecular and cellular lesion in aging and age-related diseases. Aging is characterized by progressive, irreversible and deleterious changes in tissue function. Some of the most pronounced changes occur in tissues with long-lived proteins such as in the extra and intracellular matrix, the lens and the brain. Most of these changes are dramatically worsened by diabetes. By and large, most of the changes observed in aging occur twice faster in diabetes. The importance of the Maillard reaction in aging of living organisms has been appreciated only recently. In the advanced stage of this reaction, advanced glycation endproducts(AGEs) accumulate in tissues as a function of time and sugar concentration. AGEs also induce permanent abnormalities in extra and intracellular matrix component function, stimulate cytokine and reactive oxygen species production through AGE-specific receptors, and modify intracellular proteins.

벼 키다리병 방제를 위한 차아염소산나트륨 이용

신동범,고재덕,이봉춘,강인정,강항원 한국식물병리학회 2014 식물병연구 Vol.20 No.4

For application of sodium hypochlorite as a seed disinfectant to the control of bakanae disease caused by Gibberella fujikuroi in rice, we investigated the effects of sodium hypochlorite for antifungal activity, eliminating fungus from seeds and reducing disease occurrence in vitro and greenhouse. The viability of the pathogen was significantly reduced at 80 ml/l concentration of sodium hypochlorite, and the pathogens did not grow at over 100 ml/l concentration of sodium hypochlorite. The effect of eliminating fungus was 90% at treatment of 0.3% sodium hypochlorite solution to infected rice seeds for eight hours. When the rice seeds were soaked into 0.5% and 0.3% sodium hypochlorite solutions for twelve hours, the disease incidences of rice seedling were remarkably reduced to 4.3% and 4.7%, respectively, compared to 97.3% of non-treatment control. The rates of seedling stand were 29.1% and 26.9% higher with the sodium hypochlorite treatment than that of non-treatment control. When prochloraz and sodium hypochlorite was treated to naturally severely infested rice seeds with bakanae disease, the disinfection effect was higher than that of prochloraz alone treatment. When the seeds were soaked in sodium hypochlorite before or after prochloraz, the rate of seed contamination was low as 4.0% or 6.3%, respectively, compared to prochloraz alone as 13.7%. The disease incidence was low as 3.7% or 8.3%, respectively, compared to prochloraz alone as 14.3%. The disinfection effect of treatment with prochloraz after sodium hypochlorite was higher than that of treatment with prochloraz before sodium hypochlorite.

스케일불변 특징의 삼차원 재구성을 통한 이동 로봇의 상대위치추정

신동범,길세기,이종실,유제군,이응혁,홍승홍 대한전기학회 2006 전기학회논문지 D Vol.55 No.4(D)

- A key component of autonomous navigation of intelligent home robot is localization and map building with recognized features from the environment. To validate this, accurate measurement of relative location between robot and features is essential. In this paper, we proposed relative localization algorithm based on 3D reconstruction of scale invariant features of two images which are captured from two parallel cameras. We captured two images from parallel cameras which are attached in front of robot and detect scale invariant features in each image using SIFT(scale invariant feature transform). Then, we performed matching for the two image's feature points and got the relative location using 3D reconstruction for the matched points. Stereo camera needs high precision of two camera's extrinsic and matching pixels in two camera image. Because we used two cameras which are different from stereo camera and scale invariant feature point and it's easy to setup the extrinsic parameter. Furthermore, 3D reconstruction does not need any other sensor. And the results can be simultaneously used by obstacle avoidance, map building and localization. We set 20cm the distance between two camera and capture the 3frames per second. The experimental results show ±6cm maximum error in the range of less than 2m and ±15cm maximum error in the range of between 2m and 4m.

답리작 상치 시설재배지에서의 균핵병 발생생태에 관한 연구

신동범,이준탁,Shin Dong Bum,Lee Joon Tak 한국식물병리학회 1987 Plant Pathology Journal Vol.3 No.4

답후작으로 시설재배되는 상치에 발생한 균핵병의 발생생태에 대하여 조사연구한 결과, 상치 균핵병은 전생육기간을 통하여 발생하였는데, 특히 유묘기에 피해가 심하였다. 후병지$(30\times30\times5cm)$내의 균핵수는 유묘기에 22.0개, 수확기에는 5.3개가 검출되었다. 이상치 균핵병 (S. sclerotiorum)의 균사생장적온은 $25^{\circ}C$였고, $15^{\circ}C$이하의 온도에서는 $20^{\circ}C$이상의 온도에서보다 균핵의 형성수는 적었으나 큰 균핵을 형성하였고 건물중도 높았다. 토양깊이 및 시기별로 균핵을 매몰하였을 때 3월, 4월 9월의 매몰구에서는 토양깊이 3cm구까지 자양반을 형성하였으나, 6월, 12월의 매몰구에서는 90일간의 조사기간내에는 자 반을 형성하지 않았다. 그리고 중력수만 제거한 토양과 담수토양내에서 균핵을 $25^{\circ}C$와 $30^{\circ}C$로 5주간 처리하였을 때 $100\%$의 균핵이 사멸하였다. 고압멸균된 상치 식물체절편과 함께 균핵을 상치에 접종하였을 때 균핵의 균사형성에 의한 감염이 일어났으며, 포장주위식물에 대한 병원성 조사에서는 조사식물 9과 31종중 화본료 식물을 제외한 8과 25종의 식물에 병원성을 나타냈다. 그러므로 답리작으로 시설재배되는 상치에 발생한 균핵병의 전염원은 포장주위의 기주식물에 형성된 균핵이라고 생각된다. Incidence of lettuce drop was observed throughout the growing season in the vinylhouse at the southern part of Korea, Kimhai. Occurrence of this disease was especially severe at the seedling stage. Number of sclerotia in surface soil $(30\times30\times5cm)$ was 22.0 at the seedling stage, and 5.3 at harvest in the infected area. Temperature for mycelial growth ranged from 5 to $30^{\circ}C$ with optimum temperature at $25^{\circ}C$. Sclerotia were formed fewer at low temperature, but their size was larger resulting in heavier dry weight than that at high temperature. The apothecia were formed from the sclerotia that were buried in March, April and September upto 3cm soil depth, but formed from those buried only 1 em soil depth in October. Sclerotia buried in June and December did not form apothecia regardless of soil depth by 90 days. The sclerotia buried in the 5cm of soil depth did not form apothecia. Sclerotia that were embedded in wet or flooded soil at $25^{\circ}C$ and $30^{\circ}C$ for 5 weeks lost their viability. Infection of lettuce was possible with mycelia originated from sclerotia on autoclaved lettuce plant fragments. The fungus was pathogenic on 25 plant species in 8 families in artificial inoculation tests. Lettuce seedlings appeared to be infected by airborne ascospore originated from sclerotia on crops and weeds around paddy fields, because sclerotia existing in soil might perish under long flood conditions during rice cultivation.

Sclerotinia sclerotiorum에 의한 시설재배 들깨 균핵병 발생특성

신동범,송석보,문병주 한국식물병리학회 2006 식물병연구 Vol.12 No.3

Sclerotinia rot caused by Sclerotinia sclerotiorum frequently causes serious and unpredicable yield losses of the leaves of perilla growing under structured cultivation as vegetable in Korea. Temperature for mycelial growth ranged from 5 to 30oC with optimum temperature at 20oC. Sclerotia were formed fewer at low temperature, but their dry weight was heavier than that at high temperature. The apothecia were formed from the sclerotia that buried up to 3 cm soil depth at 15oC in moisture condition. The incidence of perilla Sclerotinia rot caused by S. sclerotiorum was observed throughout the growing season at greenhouse. The occurrence of this disease was especially severe from January to February of low temperature period. The average incidence rates of this disease was up to 15%. The significant occurrence of this disease was showed mainly in the continuous cropping field for more than five years. The incidence of this disease increased according to the increase of continuous cropping year. The incidence rates of this disease reached up to 20% in the continuous cropping field for ten years. Also it was firstly investigated the natural infection caused by S. sclerotiorum on weed plants, Gnaphalium affine and Mazus pumilus in farmer's field. The casual fungus showed pathogenicity on 11 weed plants species tested, and more severe pathogenicity on G. affine, Latuca indica and Ixeris dentata included in the family Compositae. This result suggests that effective crop rotation and weed eradication can be the method for organic control of perilla sclerotinia rot, and sudden outbreaks of this disease on perilla growing under structure after paddy rice can be explained by the presence of weed hosts.