이질균의 R Plasmid의 유전적 특성

서민호,서성일,박종욱 啓明大學校 醫科大學 1988 계명의대학술지 Vol.7 No.2

In order to choose adequate antimicrobial drugs for shigellosis and to know the mechanisms of durg resistances and molecular epidemiologic data, 67 strains of Shigella flexneri and 11 strains of Shigella sonnei were tested for the antimicrobial susceptibilities, conjugal transfer of R plasmids, incompatibility grouping, plasmid DNA profiles and the recombinations of resistance genes. In S. flexneri, 88.1 to 97% of the strains were susceptible to kanamycin(Km) and rifampicin(Rf), 76.1% of the strains were susceptible to nalidixic acid(Na) and 37.3 to 52.2% of strains to sulfisomidine(Su), trimethoprim(Tp), and cotrimoxazol(Co). In S. sonnei, 100% were susceptible to Km and Rf, and 63.6% to Na. There was no susceptible strain to Cm, Tc, Sm, and Su. In contrast to S. flexneri, many strains(81.8%) of S. sonnei were susceptible to Ap and few(0 to 9.1%) strains to Su, Tp, and Co. Most of drug resistances except to Na and Km in 50 to 100% of resistant strains were co-transferred to recipient E. coli RG488, indicationg that multiple drug resistances were R plamid mediated phenomenon. Most of R plasmids were 62.4 mega dalton(Mdal) in size and classified into the incompatibility group FⅡ. Somewhat larger plasmids, 115.6Mdal in size were frequently found in Shigella. Doubles containing both resistances of incoming plasmid and resident plasmid in incompatibility grouping were tested for the plasmid DNA profiles and the conjugations to the plasmid free recipient. According to these results, we fround that doubles had single recombinant plasmid, 64.5 Mdal in size and the mechanisms of the recombination of these resistance genes were thought to be transpositions between two plasmids.

다중시기 위성영상을 이용한 시화 방조제 내만 식생변화탐지

정종철,서영상,김상욱 한국환경과학회 2006 한국환경과학회지 Vol.15 No.4

부분 신절제 백서모델에서 Angiotensin Converting Enzyme Inhibitor 및 Angiotensin II ATI 수용체 길항제가 Plasma Renin Activity 및 Angiotensin II level에 미치는 영향

김종학,구영선,강민규,황평주,나기량,이강욱,서광선,신영태 충남대학교 의과대학 지역사회의학연구소 1999 충남의대잡지 Vol.26 No.1

Renin-angiotensin-system(RAS) has been thought to have a pivotal role in renal injury mechanism. Many reports state that the inhibition of RAS prevents the progression of renal disease in 5/6 nephrectomized rats as a typical chronic renal failure model. Angiotensin converting enzyme inhibitor blocks the conversion from Angiotensin I to Angiotensin Ⅱ (A-Ⅱ ), and ATI RA inhibits the action of A-Ⅱ at the level of AT1 receptor. Therefore the PRA and A-Ⅱ level may be influenced independently by each treatment modality with these drugs. In this study, the influence of long term treatment with ACEI or AT1RA in these models on systolic blood pressure, PRA, and A-Ⅱ level was evaluated. Male Sprague-Dawley rats weighing 270-300 grams were anesthesized with thiopental sodium(50 mg/kg) and underwent right nephrectomy and partial(approximately two thirds) infarction of the left kidney by ligation of two-three segmental arterial branches. The rats were divided into four groups : sham group, control group, 5/6 subtotal nephrectomized and ACEI treated group (enalapril 100 mg/L in drinking water), 5/6 subtotal nephrectomized and ATIRA treated group (losartan, 200 mg/L in drinking water). They were treated for twelve weeks. In the twelve weeks, both groups treated with ACEI and AT1RA ingestion demonstrated a significant decrease in systolic blood pressure(165±23 vs. 132±9, mmHg, control vs. ACEI, M±SEM, p<0.05, 165±23 vs. 124±7 mmHg, control vs. ATIRA, M±SEM, p<0.01) compare to the control group. In both group treated with ACEI and AT1RA showed a significant increase in PRA(ACEI ; 7.2±2.9, AT1RA ; 4.7±0.4, control ; 2.7±1.1, sham ; 2.7±1.4, ng/ml/hr, ACEI vs. control and sham, M±SEM, p<0.05). However no significant differences were found in the sham vs. control(p>0.05), and in ACEI vs. control. The plasma A-II level was significantly increased in AT1RA treated group compared to sham and ACEI treated group(2,753±543 vs. 484±169.3, ng/ ml, AT1RA vs. Sham, p<0.01). In conclusion, treatment with ACEI and AT1RA for twelve weeks normalized systolic blood pressure, proteinuria and increased PRA compared to the control group; whereas plasma A-II level was increased only by AT1RA treatment.

HFC-134a와 대체냉매 HFC-152a에 CF_3I를 혼합한 자동차냉매 특성

권일욱,박찬수,홍경한,문기선,최운수,서의경,윤갑식,이종인,하옥남 朝鮮大學校 機械技術硏究所 2002 機械技術硏究 Vol.5 No.1

These days, an attention of environment has been increasing all over the world. to prevent the green house effect and destruction of an ozone layer, an ozone destruction potential(ODP) be zero and a refrigerant for low global warming potential(GWP) is needed. HFC-134a, in which hydrogen is mixed instead of chlorine is a refrigerant used for automobile conditioners and its destruction potential is ecologically zero. However, it is not considered as a perfect substitutive refrigerant as its GWP is high. It is studied refrigerant mixtures in which HFC-134a, HFC-152a and CF_3I with low GWP and zero ODP are mixed by experimentally and concluded as follows; 1. with the variation of speed of compressor outside temperature and flow rate, the heat of evaporator and compressor and coefficient of performance was varied, and influenced the air conditioner. 2. The pressure of evaporator was decreased with increasing the speed of compressor and that of the refrigerant HFC-134a was higher 21% than that of azotrope refrigerant mixed with CF_3I. The overall capacity of condensor and evaporator was increased and HGC-134a was higher than azotrope refrigerant mixed with CF_3I 3. The capacity of evaporator with the refrigerant HFC-134a was higher 6% than azotrope refrigerant mixed with CF31 and the required power with zaotrope refrigerant mixed with CF_3I was higher 11% than that of HFC-134a. 4. The COP of the refrigerant FHC-134a was higher 31% in 1000RPM. when outside temperature was increased, COP was decreased. 5. Performance of HFC-134a is better than azotrope refrigerant mixed with CF_3I but prefered zaotrope refrigerant mixed with CF_3I considering the environment.

성인 막성신병증의 임상적 고찰

양종오,이상주,박기현,장윤경,이강욱,서광선,신영태 충남대학교 의학연구소 2001 충남의대잡지 Vol.28 No.2

Membranous nephropathy (MN) is a glomerular disease characterized by diffuse thickening of the glomerular basement membrane without significant mesangial proliferative change and is usually manifested by the nephrotic syndrome. To evaluate the clinical and pathologic characteristics of this disease in adults, we analyzed 65 patients with primary membranous nephropathy who were diagnosed at Department of Internal Medicine, Chungnam National University Hospital from February 1986 to February 2001. The results are as follows: 1) Of total 96 patients with membranous nephropathy, 67.7% was primary MN. Hepatitis B-associated MN and lupus MN were 22.9%, and 9.4%, respectively. Median duration of follow-up was 35.1 months(0.3∼177) in primary MN, 49.6 months(2.5∼103) in lupus MN, and 35.6 months(0.5∼108) in hepatitis B-associated MN. 2) Mean age of the patients with primary MN at the time of diagnosis(43.3 years) was significantly higher than that of lupus MN(30.4 years) and HBV-associated MN(36.2 years). Male to female ratio of primary MN was 1.03:l. 3) At the time of diagnosis, 89.2% of primary MN was presented with nephrotic syndrome. Twenty seven percent of patients showed hypertension. Hematuria was found in 58.5% of primary MN patients. Azotemia was noted in 3.1 %. 4) Global sclerosis, tubular atrophy and interstitial fibrosis were noted in 39.8, 16.1 and 11.3% of primaty MN, respectively. IgG and C3 were deposited on the glomerular capillary loop in 92.7% and 29% of primary MN patients. There was no significant correlation between the electron microscopic pathologic stage and clinical findings. 5) Of 56 patients with primary MN followed more than 6 months, 50(90.9%) patients were treated with prednisolone, cyclophosphamide or cyclosporin. Complete remission was obtained in 29 patients(51.8%), partial remission in 6 patients(10.7%), and no response in 18 patients(32.1%). Of 6 patients who were not treated with immunosuppressive drugs, 3 patients showed spontaneous complete or partial remission. Three patients who did not show clinical improvement progressed to end-stage renal failure. There were no significant clinical difference including renal pathology at the time of diagnosis between the patients who showed complete or partial remission and patients who did not. In conclusion, it was difficult to predict the long-term prognosis of primary MN at the time of diagnosis, clinically. The response to therapeutic modality would be the most important to predict long-term prognosis of primary MN.

BIPV 건물 외피시스템 개발 : Curtain Wall의 일체화 가능성을 중심으로 Focused on the unification possibility to the Curtain Wall

임종욱,오민석,김회서 대한건축학회 2004 대한건축학회논문집 Vol.20 No.2

PV system(Photovoltaic system) as an alternative energy resource for a fossil fuel is judged to have an enormous possibility in a usable point of earth, everywhere. Therefore, this paper intends to advance its practical use by proposing how to get integrated PV system which can be applied to building external curtain wall, and how to apply it. It has materialized the possibility of integrating details which can be applied to curtain wall by making insulating glass PV (the performance of insulation has been improved) through adding the layer of air (12 ㎜) and rear glass (6 ㎜) to integrated sandwich glass PV module. The proposed insulating glass PV module was applied to vision and then, the load was measured. The result shows that because of influence by solar radiation to the southern part in summer, it is advantageous for energy saving and it can make an additional effect of peak cut.

동구북구산 Mesochorinae 아과(별목 : 맵시벌과)의 계통분류학적 연구 1. : Mesochorinae 속의 재고찰 A Review of the Genus Mesochorus

이종욱,최원영,서경인 고려대학교 한국곤충연구소 1997 昆蟲硏究誌 Vol.23 No.1

동구북구산 Mesochorus속을 재고찰한 결과 4미기록종을 포함하여 총 31종을 확인하였다. 따라서 동구북구산 Mesochorus속은 본 연구에서 확인하지 못한 10종을 포함하여 총 41종이 분포하는 것으로 보고된다. 원기재와의 차이점을 추가하여 기록하였고, 채집지, 분포, 숙주목록 등을 정리하였으며, 확인된 31종에 대한 검색표를 작성하였다. 이 중 Lee와 Suh의 M. sp.1은 본 연구에서 신종임이 확인되어 Mesochorus seorakensis로 새로이 명명하였다. 종목록은 아래와 같다. Aa a result of systematic study of the Mesochorinae, a total of 31 species was confirmed in the Eastern palearctic Mesochorus. Among them, four species, M. confus, M. fuscicornis, M. rubranotatus and M. uniformis are new to Eastern palearctic regional fauna. Mesochorus sp. 1 of Lee and Suh (1991) is confirmed as new species and it named to be M. seorakensis. Thus, the Eastern palearctic Masochorus is composed of 41 species including 10 unconfirmed species in this study. Additional descriptions, collection sites, distributions, host lists, keys are provided for the species.

신장조직 손상의 진행에 있어서 Renin-Angiotensin System 의 역할에 관한 연구

이강욱,송정헌,강민규,황평주,김종학,나기량,서광선,박정규,신영태 대한신장학회 2001 대한신장학회잡지 Vol.20 No.3

전신성 홍반성 낭창의 임상적 고찰

신영태,김종학,장윤경,양종오,구영선,강민규,황평주,나기량,이강욱,서광선 충남대학교 의과대학 지역사회의학연구소 1998 충남의대잡지 Vol.25 No.1

The epidemiology, diagnostic criteria, clinical features, symptoms and signs laboratory findings, kidney pathology, and clinicopathologic correlation of systemic lupus erythematosus(SLE) were analyzed. The 63 patients studied were managed at the Department of Internal medicine, CNUH, from January 1983 to December 1997. Kidney biopsy was performed in 53 patients out of 63 patients with SLE. The results were as follows: 1. The ratio of male to female was 1:26. They were 12 to 71 years old and mean age was 32.1 years old. The peak age incidence was 4th decades(30%). 2. The most frequent chief complaint on admission was generalized edema. Most patients complain two or more symptoms. 3. Immunologic and renal disorders were the most frequently observed in the ARA criteria of SLE. And the positive ANA, hematologic disorder, malar rash, and arthritis, were observed in order of frequency. 4. Among the 53 patients with renal biopsy, 30 patients revealed class Ⅳ lupus nephritis(56%), class II in 12 patients(23%), class V in 8 patients(15%) and class III in 2 patients(6%). 5. The cases of lupus nephritis represented as nephrotic syndrome were high in the class IV with 68% and class V with 86%. 6. Of 30 patients who can be followed up, 5 patients resulted in death(17 % of mortality). They "were 14 to 57 years old and mean age was 28 years old. Follow up duration were from 1 month to 6 years and 3 months, and mean duration was 1 year and 1 month, but 3 cases 7. The causes of death were as follows: Two cases were due to seizure and coma resulted from CNS involvement. One was sepsis due to miliary the & pneumonia. One was dilated cardiomyopathy and heart failure due to cardiac ac involvement. And the other one was sepsis and acute renal failure resulted from cellulitis. 8. Prednisolone was used in all patients basically, and methyl-prednisolone pulse therapy, antimalarials and alkylating agents were used in some cases. In the cases of combined therapy, the activity of SLE was well controlled.

한국산 나비 目(Lepidoptera)의 발향린(Androconia)에 관한 주사전자현기경적 연구 III. 네발나비科

박원학,류성만,서경인,이종욱 嶺南大學校 基礎科學硏究所 1992 基礎科學硏究 Vol.12 No.-

한국산 네발나비과에 보고된 77종 중 북한산 12종과 채집하지 못한 21종을 제외한 44종, 670여 개체를 재료로 광학현미경으로 발향린의 유무 및 분포를 확인하였다. 이중 발향린이 관찰된 10종을 대상으로 주사전자현미경으로 그 형태를 비교 연구하였다. 1. 발향린이 관찰된 종은 Brentis ino(R.), Argyronome laodice (P.). A. ruslana (M.). Damora sagana (D.), argynnis paphia (L.), Childrena zenobia (L.), Speyeria aglaja (L.), Fabriciana pallescens (B.), F. adippe (L.), F. nerippe(c.et R. F.) 10종이다. 2. 발향린은 암컷에서는 관찰되지 않았고, 수컷이 앞날개 표면에서만 관찰되었으며, 시맥위에서만 분포한다. 3. 확인, 관찰된 10종의 발향린은 나발막(fimbriae), 경부(neck), 잎새(laminar), 자루병반(foot stalk) 등 발향린의 전형적인 모양을 나타내었으며, 동종내 개체간 변이는 관찰되지 않았다. 4. Argyronome laodice, A. ruslana, Damora sagana, argynnis paphia, Childrena zenobia, Fabriciana pallescens 등 6종에서는 2가지 형태의 발향린이 관찰되었다. 5. 본 연구에서 관찰한 10종은 Subfamily Argynninae아과의 Argynnini족에서만 관찰되었으며, 관찰된 발향린중 Type ⅠⅠ의 형태는 종간에 큰 차이점이 없었고, Type Ⅰ은 많은 형태적 차이를 보이므로 분류학적으로 중요한 형질이라 사료된다. this paper observed through microscope the existance and the distribution of the androconia of the 44 species Nymphalidae among 77 ones that have been reported in Korea, excluding 12 species in North Korea and 21 one's not collected ten species that were observed the androconia among those were chifely compared and studied through Scanning Electron Microscope. 1. The 10 species that observed androconia is Brentis ino(R.), Argyronome laodice (P.). A. ruslana (M.). Damora sagana (D.), Argynnis paphia (L.), Childrena zenobia (L.), Speyeria aglaja (L.), Fabriciana pallescens (B.), F. adippe (L.), F. nerippe(C. et R. F.) 2. The androconia was not found at the female, but only on the dorsal surface of male's fore wings. 3. The androconia of 10 species was not observed intraspecific seasonal variation and individual variation. It was appeared the androconia as general type of fimbriae, neck, laminar and foot stalk. 4. Two types(typeⅠ and Ⅱ) of androconia were observed at the species of Argyronome laodice, A. ruslana, Damora sagana, Argynnis paphia, Childrena zenobia, Fabriciana pallescens. 5. According to this study, the androconia of 10 species observed only Argynnini, subfamily Argynniae. The type Ⅱ of the androconia similar to between species but type Ⅰ was observed multitudes of morphological difference. These difference of type Ⅰ was very important taxonomical characters.