Production and Characterization of Enzymes Involved in Chitin Catabolic Cascade from a Bacterial Strain Isolated from Soil

( Ji Hyun Lee ),( Jeong In Lee ),( So Yeon Park ),( Yoo Kyeong Jang ),( You Bin Lim ),( Hye Yoon Lee ),( Young Min Jung ),( Hae Chang Lee ),( Jong Hwa Lee ),( Shaheen Amna ),( Jae Kweon Park ) 한국키틴키토산학회 2019 한국키틴키토산학회지 Vol.24 No.1

The present study was aimed to isolate the bacteria producing chitinolytic enzymes including chitin deacetylase (CDA) from soil origin. About 24 bacteria were screened for their chitinolytic enzymes producing ability on the basis of colloidal chitin. Amongst one of the most potent isolates designated to JI-02 was further selected as the most prominent chitinolytic and CDAlike enzyme (CDA-X) producer based on the ability of deacetylation of an artificial substrate pNP-acetamilide. The maximum production of CDA-X in crude enzyme from JI-02 was observed in the presence of 1% colloidal chitin at 37°C and pH 6.4 after 120 h of incubation. Potent enzyme activity of CDA-X was determined toward pNP-acetamilide, demonstrating that optimal pH, temperature and ionic strength of the CDA-X were assessed to be 7.0, 52°C and 125 mM, respectively. Unexpectedly, Nacetylglucosamine (GlcNAc) was not fully converted by the action of CDA-X to glucosamine (GlcN) under the established conditions. Further study on enzyme activity toward chitin-oligosaccharides consisting of multi-N-acetylglucosamine (GlcNAc)n, n=2-5 may be necessary to elucidate the mode of action which requires the minimum size of (GlcNAc)n. However, our data suggest that CDA-X can convert chitin to chitosan with the maximum yield of approximately 0.08g/L during the fermentation of the strain in the presence of chitin. Furthermore, we found that crude enzyme showed the significant chitosanolytic activity. Taken together, our data suggest that the strain JI-02 may be a potential candidate as a particular strain for better understating of chitin catabolic pathway.

Do We Still Use The Camitta"s Criteria For Severe Aplastic Anemia?

( Hyun Hwa Yoon1 ),( Sung Hyun Kim1 ),( Ji Hyun Lee ),( Seok Jae Huh ),( Suee Lee ),( Sung Yong Oh ),( Hyuk Chan Kwon ),( Hyo Jin Kim ) 대한내과학회 2011 대한내과학회 추계학술대회 Vol.2011 No.-

교환계약에 있어서의 실지거래가액 과세에 관한 짧은 고찰

윤지현(Yoon Ji?Hyun) 한국법학원 2007 저스티스 Vol.- No.100

원칙적으로 실지거래가액에 의하여 과세표준을 산정하는 양도소득세제에서 특히 교환거래의 경우 실지거래가액을 무엇으로 볼 것이냐를 둘러싸고 실무상 많은 문제점이 발생하고 있다. 판례는 이에 관하여 교환거래를 ‘단순한 교환’과 시가감정 및 정산절차가 수반된, ‘단순하지 않은 교환’으로 나누고, 전자의 경우에는 실지거래가액이 존재하지 않으나 후자의 경우에는 실지거래가액이 존재하는 것으로 보고 있다. 이러한 판례의 입장은, 실지거래가액이란 당사자들이 ‘약정한 가액’이라는 전제 하에, ‘단순한 교환’의 경우 당사자들이 합의하는 것은 교환대상 자산의 절대적 가치가 아니라 상대적 교환비율에 불과하므로 실지거래가액이 존재하지 않는다고 보아야 하지만, ‘단순하지 않은 교환’의 경우에는 당사자들이 실제로 일정한 가액에 합의한 것으로 볼 수 있고 또 그러한 가액을 신빙할 수 있다는 이해에 근거를 둔 것으로 보인다. ‘단순한 교환’에 불과하여 실지거래가액을 알 수 없는 교환거래의 경우 현행법상 추계과세 대상이 된다는 명문의 규정은 없으나 다른 사유로 실지거래가액을 알 수 없는 경우에 준하여 추계과세의 대상이 되는 것으로 해석함이 타당하며, 소득세법은 추계과세의 경우 교환으로 인하여 취득하는 자산이 아닌 양도하는 자산 자체의 매매사례가액이나 감정가액 등에 의하여 양도가액을 산정하는 것으로 규정하고 있다. 다만 일반적인 추계과세의 경우와는 달리 납세자가 스스로 이와 같이 양도하는 자산의 가액을 평가하여 양도소득세를 신고납부할 수 있다고 해석하여야만 할 것이다. 궁극적으로는, 실지거래가액 과세제도가 당사자들이 실제로 얻은 양도소득을 과세대상으로 삼는 제도라는 측면에서 보았을 때, 양도소득세도 법인세법에서와 마찬가지로 교환으로 인하여 취득하는 자산의 가액에 의하여 산정하는 방향으로 전환하는 것이 바람직하며, 실지거래가액의 개념 역시 이러한 견지에서 해석하는 방향으로 나아가는 것이 해석론상, 또는 입법론상 타당한 것으로 생각한다. Under the Income Tax Act, Capital Gains Tax is levied based on "Actual Transaction Price" as opposed to "Government-Announced Price," but there is controversy as to what Actual Transaction Price means in the event of an exchange transaction. The Supreme Court generally defines the Actual Transaction Price as the price that has been "actually agreed on" between the parties. Based on this definition, the Supreme Court takes the position that the exchange transaction is divided into the two following categories: the "Non-Plain Exchange" where the parties have conducted valuation of the two exchanged assets and the difference in value between the two assets have been settled, and the "Plain Exchange" which is an exchange that, on the contrary, does not meet all of such requirements. The Supreme Court seems to believe that, in the event of a Plain Exchange, what the parties have agreed on is only the ratio of the exchange, and therefore there is no price actually agreed between the parties. However, in the event of a Non-Plain Exchange, the Supreme Court apparently reasons that, since there have been valuation of the assets and settlement of the difference in value, it is likely that the parties have actually agreed on the transaction price, which is reliable to a sufficient degree for capital gains tax purposes. Under the current Income Tax Act, the Plain Exchange where there is no Actual Transaction Price is subject to "Taxation based on Projection," where the value of the transferred asset rather than the asset received as a result of the exchange is taken into consideration in computing the capital gains tax. However, unlike in ordinary Taxation based on Projection, which is only the tax authorities' prerogative, here the taxpayer should also be able to file his or her tax return using the same method. In the end, considering that the taxation based on Actual Transaction Price is such a system where the "actual" capital gain is measured and used as basis for taxation, I believe it is more reasonable to compute capital gains tax based on the fair market value of the asset received through an exchange. I therefore believe that the Supreme Court should reverse its former position and rule that the Actual Transaction Price in an exchange is the fair market value of the asset received through an exchange, and if that is not feasible, then the legislative body should take the initiative to change the law to that effect.

축구선수의 Detraining과 Retraining이 혈청지질 및 호르몬농도에 미치는 영향

정정화,박재현,채종훈,성혜련,황지인,윤미숙,노금선,윤종관,윤영학,노순덕,정경숙,박일규,김은희,박현태,박상갑 대한스포츠의학회 1999 대한스포츠의학회지 Vol.17 No.1

The purpose of this study was to investigate the effects of detraining and retraining on serum lipid and hormones in soccer players. Subjects were seven male high-school soccer players. V˙O_2max was determined for each subjects by administering a treadmill test(initial speed: 90m/min, grade: 5%, increasing speed per 3 min: 30m/min). Serum lipid(T-C, TG, HDL-C LDL-C) and hormones(epinephrine, norepinephrine, growth hormones, cortisol) were assayed pre and post detraining in 10, 20, 30 days after retraining. The repeated ANOVA was used to determine significant differences. The 0.05 level of significance was as critical level for the study. The results of the study were as follows: 1. V˙O_2max(ml/min) were 3576.3±204.2ml/min pre detraining, 3234.1±198.9 ml/min post detraining. There are significant(p<.05) difference between pre and post detraining. In 10, 20, 30 days after retraining, V˙O_2max(ml/min) were 3601.4±170.9 ml/min. There were significantly(p<.05) increased in retraining periods. 2. V˙O_2max(ml/kg/min) were significantly(p<.05) decreased from 62.3±2.9 ml/kg/min to 55.9±4.7 ml/kg/min in detraining. In 10, 20, 30 days after retraining, V˙O_2max(ml/kg/min) were 62.4±3.4ml/kg/min, 62.7±2.3ml.kg/min, 67.3±7.2ml/kg/min respectively. There were significantly(p<.05) increased in retraining periods. 3. T-C were significantly (p<.05) increased from 166.6±8.5mg/dl to 175.3±10.3 mg/dl in detraining. In 10, 20, 30 days after retraining, T-C were 160.1± 3.2mg/dl, 156.7±3.7mg/dl, 140.3±9.0mg/dl. There were significantly(p<.05) decreased in retraining periods. 4. HDL-C were 61.4±6.6mg/di pre detraining, 5.3±6.6mg/dl post detraining. There are significant(p<.05) difference between pre and post detraining. In 10, 20, 30 days after retraining, HDL-C were 56.9±7.1mg/dl, 56.4±9.2mg/dl, 57.7±9.1mg/dl respectively. There were no significant difference in retraining periods. 5. The hormones(epinephrine. norepinephrine, growth hormone, cortisol) were changed as same patterns. Epinephrine were 26.0±7.0[g/ml pre detraining, 24.6±3.2pg/ml post detraining. In 10, 20, 30 days after retraining, epinephrine were 26.9±5.6pg/ml, 30.6±6.2pg/ml, 29.4±5.6pg/ml respectively. There were no significant difference in retraining periods. In conclusion, HDL-C, epinephrine, norepinephrine, growth hormone and cortisol were decreased, T-C, LDL-C and TG were increased in detraining. But HDL-C, epinephrine, norepinephrine, growth hormone and cortisol were increased, T-C, LDL-C and TG were decreased in retraining.

당뇨병의 유병기간과 아연 및 구리 영양상태와의 관련성

윤진숙,오현미,윤지영 啓明大學校 生活科學硏究所 2003 科學論集 Vol.29 No.-

Literature suggest a relationship between trace elements status and diabetic mellitus. This study was intended to examine the zinc and copper status of diabetic patients. Subjects who participated in this study 79 type H diabetic patients and 77 normal adults. The mean of diabetic duration was 9 years. The mean age of diabetic patients and control group was 57.4 years and 55.2 years, respectively. Zinc and copper intake was measured by food record and food frequency method. Urinary zinc and copper was assessed. There was no significant difference of zinc and copper intake between diabetic patients and control group. Urinary zinc and copper of diabetic patients was significantly higher than control group. It appeared that the longer the diabetic duration, the lower the zinc intake. We concluded that dietary information to increase zinc intake should be included for nutrient education of diabetic patients.

손마사지가 허혈성 심질환 환자의 불안에 미치는 효과

현경선,이향련,공송심,윤경자,김현섭,김효남,최지원,김운정 성인간호학회 2001 성인간호학회지 Vol.13 No.2

The purpose of this study was to investigate the effect of a hand massage program on anxiety, vital sign and pain in clients with ischemic heart disease. The design utilized for this stuffy was quail-experimental with a nonequivalent control group non-synchronized design The subjects were fifty-four patients, twenty-eight for the experimental and twenty-six for the control group, who were admitted with ischemic heart diease at a cardiac intensive care unfit in K medical center of K university. This study was carried out from May, 1999 to March, 2000. The level of anxiety and pain measured by Visual Analogue Scale, systolic blood pressure, diastolic blood pressure and pulse rate were measured before and after hand massage, the state of Anxiety was measured by the Spielberger (1970) scale at admission and after hand massage for three days. The collected data were processed by using the SPSS PC program and analyzed using χ2-test and t-test. The result of this study are as follows : 1. The scores of VAS anxiety and State of anxiety of the experimental group were lower than those of the control group. 2. The degree of systolic blond pressure, diastolic blood pressure and pulse rate of the experimental group were lower than that of the control group. 3. The score of VAS pain of the experimental group was lower than that of the control group. The results suggested that hand massage can decrease VAS anxiety, State of anxiety, vital signs and VAS pain of patients who were admitted with ischemic heart disease at cardiac intensive care unit Therefore, It Is proposed that hand massage is an appropriate nursing intervention to relieve anxiety of the patients who were admitted with ischemic heart disease at a cardiac intensive care unit.

공정 Al-Si 합금의 개량처리에 따른 조직관찰 및 기계적 특성에 관한 연구

윤지현,설은철,이광학 울산대학교 2002 공학연구논문집 Vol.33 No.2

주조용 Al 합금(AC4B)을 주단조법에 적용하기 위해 먼저 Sr과 TiB를 첨가하여 주조성 및 단조성이 우수한 주단조 소재를 개발하고 그 기계적 특성을 평가하였다. 먼저, 중력주조시 조대하게 정출하는 침상의 공정 Si를 개량처리하기 위해 공정 Si 개량 화제인 Sr 모합금을 0.025~0.1wt.%로 변화시켜 첨가한 결과 침상의 공정 Si이 미세한 섬유상으로 변화한 것을 관찰할 수 있었으며, 0.075wt.%일 때 가장 우수한 개량화 효과를 나타내었다. 그러나, Sr을 첨가하지 않은 상태에서 균일하게 관찰되던 α-Al상이 Sr 첨가로 인해 조대한 수지상으로 형태가 변화한 것을 관찰할 수 있었다. 따라서, α-Al 고용체상을 미세화하기 위하여 Sr이 0.075w1.% 용해된 알루미늄 합금 용탕에 AlTi_{5}B 모합금을 0.05~0.5wt.%로 변화시키면서 첨가한 결과 α-Al 고용체상이 미세화됨을 관찰할 수 있었으며, 0.1wt.%일 때 가장 우수한 미세화 효과를 나타내었다. 한편, 개량화 및 미세화 처리한 시편의 기계적 특성 평가를 위해 인장시험과 내마모시험을 실시한 결과 인장강도, 신율 및 내마모성이 주방상태에 비해 크게 향상되는 것으로 나타났다. Recently, many studies have been carried out to process on the purpose of lightness in a transport parts because of the saving energy, the environmental problem. The cast-forging process can be expected to lower costs without decreasing the mechanical properties. So, the finest microstructure is needed to get for applying the cast-forging process with Al-Si alloy because the microstructure affects to the cast-forging process. For refinement treatment of eutectic Si and a-Al solid-solution phase, Sr and TiB were added in A-Si alloys. The finest microstructure could be observed when 0.075wt.%Sr and 0.lwt.%TiB were added respectively. In this case, tensile strength and elongation much more increased than as casting.

지구단위계획구역의 공동주택 개발 유형 비교 : 만덕지구를 중심

윤철현,김주석,강지은 東亞大學校 建設技術硏究所 2006 硏究論文集 Vol.29 No.2

The District Unit Planning(DUP) system, which was introduced in the revised Urban Planning Law in 2000, is a policy instrument for specific planning in a special area in urban setting. The main purpose of this study is to find better DUP in Man-duk in Busan. Through comparing two plans ; One is planned low buildings in Man-duk, the other is planned high buildings in the same; in Man-duk. To compare these plans, the study suggested to consider the several criteria and landscape-simulations. By comparing two DUP in Man-duk, the second plan was better for residents than the frist. DUP system is concerned with improving the urban spatial quality, but we have recognised that DUP system has some problems. There are many various types in urban area which is needed DUP system. Finally, based on this study, urban areas need the flexible application of DUP for each type and is considered the correlations of area-specific and goal-specific features. 현행 지구 단위계획의 목적은 용도지역제의 의 문제점과 한계 를 극복하고 당해 지역 토지의 효율적 이용과 주민의 안정된 주거환경 도모는 물론 인접지역과 조화를 창출하자는데 있다. 지역이 지구단위계획에 의해서 개발되면, 토지의 입체적 이용, 지구 내 기능의 적정 배분, 도시설계 상 융통성 부여, 그리고 지구 간 조화의 도모 등에 있어 무리와 경직성이 최소화 될 수 있다. 이로부터 토지의 효율적 이용에 의한 난개발 방지, 경관보전, 그리고 지역전체의 안정된 공간구성의 극 대효과를 얻을 수 있다. 최근 들어 많은 지역에서 지구단위계획의 수립이 요구되고 있으며 그 대상과 범위는 상이하다. 그러나 지구단위계획의 운용실태를 살펴보면 차별적 계획목표의 부재와 천편 일률적인 계획요소의 적용으로 많은 문제점 이 나타나고 있다- 따라서 본 연구는 하나의 연구 사례대상 지역을 선정하고 이 지역에 어떤 형태의 주거단지가 개발되는 것이 지역 전체의 조화를 창출할 것인지 를 파악코자 한다. 또한 주어진 대상지의 내적 조건상 어떤 유형의 개발이 효율적 토지이용의 최선인가 를 밝히고자 한다. 이 과정상 바람직한 지구 단위계획의 방향도 모색될 것이다.

석면함유 슬레이트 지붕 물받이 퇴적물 중 석면 섬유 함유율

임지현,한솔민,김현석,신유민,박시은,허정윤,김민영,장봉기 순천향대학교 기초과학연구소 2022 순천향자연과학연구 논문집 Vol.28 No.1,2

This study attempted to determine the degree of asbestos release from the aging slate roof by comparing the asbestos content in the slate roof rain gutter with a colored steel plate (tin plate) over the slate roof. Four slate roof houses located in Haengmok-ri, Asan-si, Chungcheongnam-do, and one house constructed with a colored steel plate on the slate roof were selected to collect the sediment of the roof rain gutter. The asbestos fiber content was calculated by a point counting method using a polarization microscope after pretreatment with conversion treatment and hydrochloric acid treatment. The average asbestos content of the four slate roof rain gutter were 1.89%. However, asbestos was not detected in the Slate covering roof rain gutter, which were constructed on the slate roof. Asbestos fiber content was the highest at 2.89% in the slate roof rain gutter installed in 1976, followed by 2.44% in 1953. From the above results, it is necessary to minimize secondary damage as asbestos fibers released from slate roof houses to the surrounding atmosphere or leaked from slate roofs as rainwater may cause soil pollution and seriously affect residents' health. Although covering with colored steel plates (tin plates) has been shown to prevent the leakage of asbestos fibers to some extent, it is believed that a policy alternative to remove the slate roof as soon as possible is needed to solve the fundamental problem.

노인정으로 출근하는 여성노인들 : 그들의 삶에 투영된 소외와 제약들 How Social Prejudice and Customs have Shackled Korean Female Seniors

지현진,정윤하,이철원 한국스포츠리서치 2004 한국 스포츠 리서치 Vol.15 No.1

The purpose of the present study was to provide a view of the lives of Korean female seniors, who visit their nearby No-In-Jung almost everyday. Especially, it was to explore why they failed to get away from No-In-Jung and make their life more dynamic. Seven daily visitors of two No-In-Jungs in Seoul were closely observed and individually interviewed. The facility and the environment composing the No-In-Jungs were also investigated. Results revealed six key factors: isolation from family, living as korean women, living as simple workers, weakened body and mind, economic dependence, and isolated from social welfare program. Insights and suggestions were also provided.