韓牛 末梢血液 淋巴球의 在來山羊 赤血球 Rosette形成

宋憙鍾,金鍾冕,姜明大,蔡孝錫 全北大學校 附設 畜産開發硏究所 1988 畜産開發硏究報告 Vol.2 No.-

In order to enumerate the T lymphocytes in bovine peripheral blood lymphocytes(PBL) by E rosette assay, KGRBC were treated with various concentration of 2-aminoethylisothiouronium bromide hydrobromide(AET) and dextran(Dex), singte or combination. The PBL from 10 clinically normal Korean native cattle were formed as low percentage of spontaneous E rosette(6.7±2.4%) in control group, whereas KGRBC treated with 0.1M AET for 20 minutes and 8%Dex were formed as 37.3±2.7% and 45.1±2.1%, respectively. And the synergistic effects were noted no less than 66.5±5.6% when the KGRBC treated with 0.1M AET and 8% Dex subsequently. These results suggest that the KGRBC would be a useful agent for the enumeration of T tymphocytes by E rosette assay in bovine PBI.

실선시험기법을 이용한 다방향파중에서의 선박의 응답추정법

조효제,강일권,김종철 韓國海洋大學校 附設 海洋科學技術硏究所 1998 硏究論文集 Vol.7 No.-

In this study, the frequency transfer function of motions are predicted from the result of a full-scale seakeeping trials. Because the real sea has the characteristics of multi-directional waves, we compare the results in the one directional waves with ones in the directional waves. For calculation of the frequency transfer function in the directional waves, Takezawa's inverse estimation method was introduced and the frequency ranges were divided into three parts in order to consider following seas. The full-scale seakeeping trials was executed in the south sea of Korea using the stern trawler. Those results show that analysis method of the multi-directional waves is more reliable than that of one directional waves, and confirm the possibility of applying this method to the full-scale seakeeping trials.

1998년과 2007년 사이 12∼13세 남녀 청소년의 체격, 골격성숙도, 체력 비교

송종국(Jong Kook Song),채서연(Seo Yeon Chae),채주희(Joo Hee Chae),김현배(Hyun Bae Kim),강효정(Hyo Jung Kang) 한국사회체육학회 2008 한국사회체육학회지 Vol.0 No.34

The purpose of the present investigation was to compare physique, skeletal maturation and physical fitness in Korean adolescents measured between 1998 and 2007. Subjects participated in the study were a total of 473 Korean adolescents aged 12 to 13 years. In 1998, 349 adolescents were selected from a middle school, located in Sungnam city and 124 middle school students aged 12-13, located in Yongin city were participated in 2007. All measurements were conducted by one of the reseachers (Jong-Kook Song) with two different group of investigators between 1998 and 2007. Anthropometric characteristics were body mass, body height, sitting height, circumferences taken at sites of upper arm, flexed upper arm, chest, waist, hip, thigh, and calf, bone breadth taken at the sites of humerus, and femur, skinfold thicknesses taken at the sites of triceps, subscapular, suprailiac, thigh and medial calf. Skeletal maturity was assessed by TW3 (Tanner et al., 2001). Maturity scores for Radius-Ulna-Short bones (RUS) and bone age were calculated. Physical fitness parameters included flamingo balance, sit and reach, grip strength, sit-ups, standing long jump, bent arm hang, 50m shuttle run and multi-stage shuttle run. All data were analyzed by SAS Enterprise 3.0. Means and standard deviations were calculated by descriptive statistics. Independent t-test was applied to compare dependent variables between measurements. The level of significance was set at p<0.05. Results of the study showed that male adolescents aged 12-13years in 2007 had significantly higher (p<0.0001) femur diameter than adolescents in 1998. Male adolescents in 2007 had higher (p<0.05) chest, waist and calf circumferences than those of adolescents in 1998. Although no difference were found for RUS and bone age in males adolescents between 1998 and 2007 female adolescents in 2007 were significantly delayed for their skeletal maturity level compared to female adolescents in 1998. For physical fitness parameters male adolescents aged 12 years in 2007 performed better for flamingo balance (p<0.01), and 50m shuttle run (p<0.05) than those of adolescents in 1998. However, stand long jump and bent arm hang were significantly decreased in male adolescents in 2007. Female adolescents aged 12-13 years in 2007 had significantly higher flamingo balance, but female adolescents aged 12 years in 2007 performed significantly less for standing long jump (p<0.0001), bent arm hang (p<0.001) and speed (p<0.05) than those of female adolescents in 1998. In conclusions this study suggest that chest circumference for both male and female adolescents in 2007 was significantly higher than those in 1998. Skeletal maturity for female adolescents in 2007 were significantly delayed compared to female adolescents in 1998. Further study is needed to evaluate consistent various.

香砂平胃散 및 加減方이 白鼠의 摘出腸管運動에 미치는 影響

宋孝元,柳道坤,韓宗鉉,康舜洙 圓光大學校 韓醫學硏究所 1992 원광한의학 Vol.2 No.1

Subtracting from component drugs by nine cases in Hyangsapyengwisan(香砂平胃散), its prescription and investigated the effect on contractile force of intestine in rats. The results obtained were as follows : 1. The effect of relaxation after contraction of rat ileum could be observed by administration of Hyangsapyengwisan. 2. By pretreatment with Atropine, the contractility of Hyangsapyengwisan was suppressed. 3. No change observed in contractile response of Hyangsapyengwisan by pretreatment with Cyproheptadine. 4. By pretreatment with Propranolol, the relaxation of Hyangsapyengwisan was suppressed. 5. The effect of relaxation after contraction of Hyangsapyengwisan was similar to that of Atractylodis Rhizoma. 6. The contractile response observed in subtracted Cyperi Rhizoma and Magnoliae Cortex from Hyangsapyengwisan, while growps in subtracted Atractylodis Rhizoma, Aurantii Nobilis Pericarpium Cyperi Rhizoma, Ponciri Fructus, Agatsachis Herba, Cyperi Rhizoma and Ponciri Fructus, Ponciri Fructus and Magnoliac Cortex from Hyangsapyengwisan showed the relaxation. These results suggest that Hyangsapyengwisan has a contractile effect which may be caused by the partial action of sympathetic and parasympathetic nervous system.

醱酵食品添加物의 能力 및 安全性確認 硏究 : -第二報 乳酸菌製劑食品의 活性能力試驗- -PartⅡ Activity Test of Lactic Acid Bacterial Food and Drugs-

鄭鎬權,姜孝源,權泰鍾 건국대학교 1978 學術誌 Vol.22 No.1

This work was carried out to investigate the acid producing activity, tolerance and other important characteristics of the strains isolated from the market yogurt drinks (fermented milk or fomented milk drinks) and lactic bacterial drugs (control agent of entero-bacteria). And obtained results were as in the followings. 1. Almost of the strains isolated from market yogurt drinks were identified as Lactobacillus bulgaricus and two of them were Lactobacillus acidophillus. The specially aerobic strains isolated from lactic acid bacterial drugs were identified as Bacillus subtilis and anaerobics were Clostridium butylicum, Sporolactobacillus inulinus, Streptococcus faecalis and Bifidobacterium bifidum. 2. Concentration of active lactic acid bacteria of the market yogurt drinks was found almost as same level, and decreasing tendency of the concentration was commonly seen in every samples during hot summer (high temperature over 35℃). Acid producing activity of some stronger strains continued for 10 days and weak strains for 6 days in summer. 3. During 6 months of the test period, not any contamination case of pathogenic enter-obacteria was found in market yogurt drink samples. 4. But yeast contamination was so aboundantly seen that the ratio of contaminated case was attained to about 20% in fermented milk and to 80% in fermented drink samples. 5. Maximum acid production of the strains isolated from market yogurt drinks was attained to almost 2.0%, but that of the strains from tactic acid bacterial drugs was less than 0.7% except the Str. faecalis strain. 6. Curd forming and acid producing activities of both L. bulgaricus strains and L. acidophillus strains were almost as same level. And the activities continued for 15 days at temperature of 5℃ and pH of 4.0, but for 7 days at high temperature of 35℃. 7. In high temperature (over 35℃) and loll pH condition (less than 3.6), the old culture of homo fermentative lactic acid bacterial strains isolated from market yogurt drinks were more easily changed to hetero fermentative strains than the young culture of the strains.

흰쥐 생체시료 중 5-플루오로우라실 및 테가푸르의 안정성

장지현,박종국,강진형,정석재,심창구,구효정 한국약제학회 2004 Journal of Pharmaceutical Investigation Vol.34 No.3

5-Fluorouracil (5-FU) is an antimetabolite anticancer agent active against many types of solid tumors. Tegafur (TF), a prodrug of 5-FU, is frequently used in combination with uracil as dihydropyrimidine dehydrogenase (DPD) inhibitory fluoropyrimidine. We studied the stability of 5-FU and TF in biological fluids of rats and determined their bioavailability (BA) and excretion into bile, and urine. The drug concentrations were analyzed by an HPLC method. At room temperature, there was a 14-30% decrease in the concentration of 5-FU and TF in bile, urine, and plasma specimen at 10 and 100 μg/ml over 240 min. No significant difference was noted among the sample types or between two different concentrations of 10 and 100 μg/ml. The decrease in drug concentration was significantly less in samples kept on ice (6-12%) for both drugs. These data indicate that biological fluid samples containing 5-FU or TF in plasma, urine, or bile should be placed on ice during the sample collection. Following these storage guidelines, samples were collected after administration 50 ㎎/㎏ of each drug via i.v. or oral route. BA was 1.5 folds greater for TF (60%) than that of 5-FU (42%). Approximately 0.52 and 3.3% of the i.v. doses of 5-FU and TF was excreted into bile, respectively. Renal clearance of 5-FU was about 16% of its total body clearance. These results suggest that instability of 5-FU and TF in biological fluids should be considered in pharmacokinetic or pharmacogenomic studies.

전북지방 젖소의 유방염 검색

조정곤,김종면,최민순,채효석,강명대,송희종 全北大學校 附設 畜産開發硏究所 1987 畜産開發硏究報告 Vol.1 No.-

A total of 444 quarters milk samples of 111 dairy cattle in Chonbuk area were examined for infection rete and causative agents of mastitis by "the laboratory procedures used in the Connecticut Mastitis Control Program (CMCP) and California mastitis Test (CMT)". The results obtained were as follows: 1. Of 444 samples, 39.2% had positive CMCP value and 24.4% positive CMT reaction. 2. One hendred and seventy four (39.2%) from 82 cattle (73.9%) were found to infected with mastitis. It was found that 8(1.8%0 of the infected quarters were clinical mastitis and all of the rest were subclinical mastitis. 3. The main causative agents were found to Staphylococcus aureus (63 quarters), Streptococcus agalactiae (56 quarters), non-Str. agalactiae streptococci (54 querters), Escherichia coli (19 quaters), the actual total number of quarters detected as mastitis would be 174, as 18 of these quarters showed multiple infection with two different organisms. 4. Having examined the number of infection quarter per head of mastitis in dairy cattle sampling, major finding were that the average number of infection quarters to total dairy cattle exemined was 1.57 quarters, and that of the infected cattle was 2.1 quarters. Infected quarters in cattle with 1, 2, 3 and 4 were noted 27(32.9%), 28(34.1%), 17(20.7%), and 10(12.3%), respectively.

乳房炎 乳汁에서 分離된 原因菌의 抗菌劑 感受性

崔民淳,金鍾冕,趙正坤,蔡孝錫,姜明大,宋熹鍾 全北大學校 附設 畜産開發硏究所 1987 畜産開發硏究報告 Vol.1 No.-

A total of 189 strains (Streptococcus agalactiae and non-Streptococcus agalactiae 110, Staphylococcus aureus 63, and E. coli 16) isolated from bovine mastitie milk samples by "The Connecticut Mastitis Control Program" in dairy herds were examined for drug susceptibility to 8 drugs and analysed the rate of multiple resistant patterns. 1. All of the isolates were resistant to antimicrobial agents such as penicillin (PC), streptomycin (SM), kanamycin(KM), carbenicillin (CA), lincomycin (LM), gentamicin (GM), and tetracycline (TC), singly or multiply. The isolates were more susceptible to CA (84.1%),PC (78.9%), and SM (78.4%) than the the other drugs such as GM (51.8%), KM (48.7%), TC (19.6%) and LM (19.0%) in order. 2. The multiple resistant isolates (96.3%) were more than those of singly resistant isolates (3.7%) and 58 different drug resistant patterns were observed. Among 7 singly resistant isolates, CP was the commonest. While among 182 multiple resistant isolates, CP-KM-LM-TC-GM, CP-LM-TC, CP-LM-TC-GM, PC-CP-SM-LM-TC-GM, CP-KM-LM-TC, and CP-LM patterns were frequently encounted. 3. The strains of streptococcus spp. were more susceptible to CA (90.1%) and SM (82.9%) than the other drugs such as KM (43.2%), GM (38.7%), LM (13.5%), CP (10.8%) and TC (10.8%). Among them CP-KM-LM-TC-CM pattern was frequently encounted. 4. The strains of Sta. aureus were more susceptible to PC (74.6%),CA (69.8%), SM (65.1%) and GM (63.5%) than the other drugs such as KM (46.0%), CP (22.2%), TC (15.9%) and LM (9.5%). Among them CP-LM-TC pattern was commonly encounted. 5. The strains of E. coli were more susceptible to CA (100%) and KM (75.0%) tnan TC (9.5%). But the other drugs such as PC, SM, GM, CP and LM were not. Among them PC-CP-SM-LM-TC-GM pattern was commonly encounted.

백서 혈관평활근 세포에서 α-Lipoic acid가 PAI-1 발현, 세포의 증식, 주유능 및 신생내막 형성억제에 미치는 효과

신동우,이동욱,이상준,김혜순,강효경,안종덕,이인규 대한당뇨병학회 2002 Diabetes and Metabolism Journal Vol.25 No.6

연구배경:당뇨병의 혈관 합병증의 발생에 있어서 산화스트레스는 중요한 역할을 하는 것으로 알려져 있다. 혈관 평활근세포에서 고혈당은 안지오텐신Ⅱ와 더불어 활성산소족(ROS)을 증가시키며, 산화스트레스에 민감한 전사인자들을 활성화시켜 동맥경화증을 유발하는 것으로 알려져 있다. 이에 저자들은 항산화제로 알려진 알파­리포산이 혈관 평활근세포의 증식속도와 이주에 미치는 영향과 PAI­1발현에 미치는 영향을 조사하였고 이와 동시에 백서의 혈관 손상 모델을 이용하여 알파­리포산이 신생내막 증식을 억제시킬 수 있는지를 알아보았다. 방법:In vitro 실험은 백서의 대동맥 평활근세포를 고농도의 포도당(22mM)과 100nM의 안지오텐신Ⅱ로 4시간 배양하였다. 알파­리포산을 처리 후, PAI­1 mRNA의 발현을 보기 위해 노던 블롯을 시행하였고 평활근세포의 유주능과 증식속도에 미치는 영향을 보기 위해 성장속도의 유주능 분석을 시행하였다. 또한 평활근세포의 유주능에 NF­μB 경로가 미치는 영향을 보기 위해 겔 지연 분석과 NF­μB 보고 유전자 분석을 시행하였다. In vivo 실험으로 백서의 혈관손상 모델을 이용하여 알파­리포산 처리 후 혈관의 신생내막 두께를 비교하였다. 결과:In vitro 실험에서 고농도 포도당과 안지오텐신Ⅱ에 의해 유도된 PAI­1 mRNA 발현증가는 알파­리포산 처리에 의하여 용량에 비례하여 억제되었다(p<0.05). 알파­리포산 처리로 혈관 평활근세포의 유주능은 유의하게 억제되었으나(p<0.01),증식속도는 유의하게 억제되지 않았다. 또한 알파­리포산 처리로 NF­μB 발현도 유의하게 억제되었다(p<0.01). In vivo 실험에서 알파­리포산을 주입한 군에서 혈관손상에 의한 신생내막의 증식이 유의하게 억제되는 것을 보여주었다(p<0.01). 결론:알파­리포산은 백서의 대동맥 평활근 세포의 증식속도는 억제하지 못했으나 유주능은 유의하게 억제하였으며, 혈관 손상 모델에서 신생 내막 증식도 유의하게 억제함을 확인할 수 있었다. 이러한 작용은 알파­리포산에 의한 NF­μB 경로의 억제와 연관이 있는 것으로 사료된다. Background : Exposure to large amounts of glucose causes a characteristic dysfunction and morphologic changes of the endothelium by an increased production of reactive oxygen species(ROS) in diabetes. The plasminogen activator inhibitor-1(PAI-1), which modulates fibrinolysis and cell migration may influence proteolysis and neointimal formation in vascular smooth muscle cells(VSMC). Antioxidants have been proposed to inhibit multiple proatherogenic events. This study investigated the effect of α-lipoic acid on PAI-1 expression and VSMC proliferation and migration both in vivo and in vitro. Methods : In the in vitro study, cultured rat aortic smooth muscle cells(RASMC) were incubated in a medium containing high glucose (22 mM) and 100 nM angiotensin Ⅱ for 4 hour. After α-lipoic acidtreatment, a -migration and growth assay of the RASMC, and a gelmobility shift assay and reportergene analysis for nuclear factor- иB(NF- иB) and northern blot analysis for PAI-1 were performed. In the in vivo study, the effect of α-lipoic acid on neointimal hyperplasia in a rat carotid balloon injury model was evaluated. Results : RASMC migration was inhibited significantly by α-lipoic acid (p<0.01), but their prolife ration was not inhibited. The NF-иB DNA binding activity and NF-иB promoter activity was inhibited by α-lipoic acid significantly (p<0.01). α-lipoic acid inhibited PAI-1 mRNA expression by high glucose and angiotensin Ⅱ in dose dependent manner (p<0.05). In the rat carotid artery balloon injury model, neointimal formation was reduced by α-lipoic acid treatment in a dose dependent manner significantly (p<0.01). Conclusion : α-lipoic acid suppresses migration, but not prolife ration in RASMC. α-lipoic acid also reduce neointima formation in a rat carotid balloon injured model. This effect might be related to the blocking of NF-иB which increase the expression of the genes associated with atherosclerosis including TNF-α, IL-1, IL-6, endothelin-1, MCP-1, VCAM-1, ICAM-1, E-selectin, tissue factor(J Kor Diabetes Asso 25:446~459, 2001).

Estrogen Prevents Activation-Induced Apoptosis by Downregulation of Fas Ligand Expression in T Cells

( Hyo Jong Kang ),( So Youn Min ),( Mi La Cho ),( Jin Jung Choi ),( Kyung Soo Park ),( Jong Hyun Yoon ),( Do June Min ),( Wan Uk Kim ),( Jun Ki Min ),( Youn Sik Hong ),( Sung Hwan Park ),( Sue Yun Hwa 대한내과학회 2001 대한내과학회 추계학술대회 Vol.61 No.-