혈장내 testosterone정량을 위한 화학발광 면역분석법 개발

박관하,김영길,우종규,최상훈,최민순 群山大學校 1997 論文集 Vol.24 No.-

The immunoassay monitored by the detection of chemiluminescence for the determination of plasma testosterone has been developed. The labelled antigen was testosterone-3-carboxymethyl oxime-aminobutylethyl isoluminol. Following an antigen-antibody reaction, the bound fraction was separated from free fraction by the addition of dextran-coated charcoal followed by a centrifugation. Sodium hydroxide (5N) was added and the mixture incubated for 1hr at 60℃. Luminescence is initiated by oxidation of the label with microperoxidase-hydrogen peroxide and the signal integrated for 10sec. The light yield was inversely proportional to the concentration of testosterone in the standard of samples. This method appeared to show a similar sensitivity, specificity, precision, and an accuracy compared to the conventional radioimmunoassay(RIA) with a tritiated antigen. The results obtained from the analysis of plasma testosterone were in good agreement with the values derived by RIA(γ=0.97)

Levamisole이 양식뱀장어의 면역조절작용 및 항균효과에 미치는 영향

최민순,박관하,조정곤 한국임상수의학회 1998 한국임상수의학회지 Vol.15 No.1

This experiment was carried out in order to evaluate the immunomodulatory activity of levamisole (LMS) in 5. fgrjn challenged eels with different treatment regimens: 7-day LMS treatment before the challenge, 7-day LMS treatment started simultaneously with the challenge, 14-day treatment before and after the challenge. The antibacterial effect was activated in all treated groups, with the best being obtained in the simultaneously treated group. LMS stimulated the defense mechanisms of the eel as demonstrated by increase in the level of total protein, albumin, trypsin inhibitor capacity, lysozyme activity, antibody titers antibacterial activity and survival rate. These results suggest that antibacterial effects of LMS was achieved by not only non-specific immune response but also specific one in eel.

원유의 노출이 담치와 조피볼락의 phase II 약물대사효소 UDP-glucoronosyl transferase 및 glutathione S-transferase의 활성에 미치는 영향

최민순,김정상,박관하,김주완,박음미,임철원,최선남,황인영 환경독성보건학회 2006 환경독성보건학회지 Vol.21 No.2

국산 내만의 해양수에서 분리되는 세균의 분포

최민순,정경민,심현빈 한국어병학회 2000 한국어병학회지 Vol.13 No.1

군산내만 해역의 해양생태계에 관한 기초자료를 얻기 위하여 금강 하구둑부터 외항까지 5개의 정점을 선정하여 종속영양 세균상의 분포를 조사하였다. 조사지역으로부터 123개의 집락을 분리-동정한 결과 Vibrio spp.(44주, 35.7%), 및 Pseudomonas spp.(42주, 34.1%), 균종이 비교적 높게 출현하였고 Aeromonas spp.(11주, 8.9%), Moraxella spp.(9주, 7.3%), Escherichia coli.(6주, 4.8%), Bordetella spp.(3주, 2.4%), Alkaligenesis spp.(3주, 2.4%), Flavobacterium spp.(2주, 1.6%), and Staphylococcus spp.(3주, 2.4%)등은 낮은 출현율을 나타냈다. 한편, 각 총 정점의 생균수는 5×10³∼1×10^5 CFU/㎖이었으며, 생활 하수가 많이 유입되는 지역(정점 2.3)이 강 하구 및 외항지역보다 높은 검출율을 보였다. 이러한 결과는 도시의 생활 오.하수 및 산업 부산물 등의 유입으로 내만의 수질 악화가 지속적으로 진행되고 있는 것으로 추정된다. 따라서 양식사업에 있어서 문제시 되고 있는 어류의 세균성 질병의 발생이 증가될 것으로 사료되어진다. This study was carried out to elucidate the preliminary ecosystem of the heterotrophic bacterial flora in Kunsan Bay located in western costal area of Korea. Samples were collected at 5 sampling stations. Among 123 bacterial isolates, 9 genera of bacteria were appeared as follows; Vibrio spp.(44 isolates/35.7%), Pseudomonas spp.(42 isolates/34.1%), Aeromonas spp.(11 isolates/8.9%), Moraxella spp.(9 isolates/7.3%), Enterobacteria spp.(6 isolates/4.8%), Bordetella spp.(3 isolates/2.4%), Alkaligenesis spp.(3 isolates/2.4%), Flavobacterium spp.(2 isolates/1.6%), and Staphylococcus spp.(3 isolates/2.4%) respectively. Total viable heterotrophic bacteria was ranged from 5×10³ to 1×10^5 CFU/㎖ and the most abundant viable counts of bacterial population were showed at the stations 2 and 3. This result indicates that the coastal area around Kunsan bay is getting to contaminate far more by municipal wastewaters and industrial byproducts. and so the outbreak of the bacterial diseases will be increased in fish farms.

in vitro 에서 Quaternary Ammonium Compounds ( QACs ) 이 뱀장어의 면역반응에 미치는 영향

최민순 한국어병학회 2000 한국어병학회지 Vol.13 No.2

Quaternary ammonium compounds(QACs)가 뱀장어의 면역반응에 미치는 영향을 측정하기 위해서 뱀장어의 혈액으로부터 림프구를 분리하여 증식능을 측정하였으며 또한 신장으로부터 탐식세포를 분리하여 탐식능, pinocytosis 및 superoxide anion활성능 등을 측정하였다. 림프구의 증식능은 mytogen(T-cell mitogen: ConA and PHA, B-cell mitogen: LPS) 및 QACs처리농도에 따라 별다른 차이를 보이지 않았다. 한편 신장 유래의 대식세포의 세균살해능은 QACs첨가농도에 따라 유의성 있게 증가되었다. 이러한 결과는 QACs이 작동대식세포로의 분화 및 활성을 촉진시켜서 탐식능, pinocytes 및 superoxide생성 등의 분비를 증가시킨 것으로 사료된다. 이상의 결과로 미루어 QACs가 어체의 면역반응에 관여하는 작용기전은 면역작동세포의 수를 증가시키기보다는 세포의 기능활성을 증강시킴을 알 수 있다. The immunostimulatory effects of quaternary ammonium compounds(QACs) were investigated in leucocytes of eel(Anguilla japonica) in vitro. Proliferation of peripheral blood lymphocytes(PBLs) was no significantly affected by QACs, regardless of mitogen(PHA, ConA and LPS) and the concentration of QACs added. QACs heightened the leucocytes function such as respiratory burst activity, phagocytosis and pinocytosis, resulting in significantly increased the bactericidal activity of macrophages. These results suggested that QAC might modulate the immune responses by activation of leucocytes function but not by increment of immunocompetent cell numbers.

乳房炎 乳汁에서 分離된 原因菌의 抗菌劑 感受性

崔民淳,金鍾冕,趙正坤,蔡孝錫,姜明大,宋熹鍾 全北大學校 附設 畜産開發硏究所 1987 畜産開發硏究報告 Vol.1 No.-

A total of 189 strains (Streptococcus agalactiae and non-Streptococcus agalactiae 110, Staphylococcus aureus 63, and E. coli 16) isolated from bovine mastitie milk samples by "The Connecticut Mastitis Control Program" in dairy herds were examined for drug susceptibility to 8 drugs and analysed the rate of multiple resistant patterns. 1. All of the isolates were resistant to antimicrobial agents such as penicillin (PC), streptomycin (SM), kanamycin(KM), carbenicillin (CA), lincomycin (LM), gentamicin (GM), and tetracycline (TC), singly or multiply. The isolates were more susceptible to CA (84.1%),PC (78.9%), and SM (78.4%) than the the other drugs such as GM (51.8%), KM (48.7%), TC (19.6%) and LM (19.0%) in order. 2. The multiple resistant isolates (96.3%) were more than those of singly resistant isolates (3.7%) and 58 different drug resistant patterns were observed. Among 7 singly resistant isolates, CP was the commonest. While among 182 multiple resistant isolates, CP-KM-LM-TC-GM, CP-LM-TC, CP-LM-TC-GM, PC-CP-SM-LM-TC-GM, CP-KM-LM-TC, and CP-LM patterns were frequently encounted. 3. The strains of streptococcus spp. were more susceptible to CA (90.1%) and SM (82.9%) than the other drugs such as KM (43.2%), GM (38.7%), LM (13.5%), CP (10.8%) and TC (10.8%). Among them CP-KM-LM-TC-CM pattern was frequently encounted. 4. The strains of Sta. aureus were more susceptible to PC (74.6%),CA (69.8%), SM (65.1%) and GM (63.5%) than the other drugs such as KM (46.0%), CP (22.2%), TC (15.9%) and LM (9.5%). Among them CP-LM-TC pattern was commonly encounted. 5. The strains of E. coli were more susceptible to CA (100%) and KM (75.0%) tnan TC (9.5%). But the other drugs such as PC, SM, GM, CP and LM were not. Among them PC-CP-SM-LM-TC-GM pattern was commonly encounted.

2000 년도 한국수의공중보건학회 추계학술대회 및 정기총회 - 포스터 : 군산내만에서 분리한 세균총의 약제 내성

최민순,조정곤 한국수의공중보건학회 2000 한국수의공중보건학회 추계학술대회 Vol.- No.-

뱀장어 아가미 울혈증에 관한 연구 III. NaCl처리가 울혈증 치료에 미치는 영향

최민순,박관하,최상훈,김영길,김종면,조정곤,장선일,Choi, Min-Soon,Park, Kwan-Ha,Choi, Sang-Hoon,Kim, Young-Gill,Kim, Jong-Myeon,Cho, Jeong-Gon,Jang, Seon-Il 한국어병학회 1995 한국어병학회지 Vol.8 No.2

This experiment was carried out in order to elucidate the effect of NaCl(0.85%) acclimation on congestion symptoms in central venous sinuses(CVS) of gill filaments and on the alteration of blood constituents in cultured eels for 1, 2, 3, 4, 5, 6 and 7days after handling stress. After giving a handling stress on eels, the frequency of gill congestion was about 90%. It gradually decreased to 40% on the 3rd day, then increased to 60% on the 7th day in NaCl(0.85%) acclimation. The values of haematocrit(Ht), methemoglobin(Met-Hb), glucose (GLU), calcium($Ca^{++}$), lactic dehydrogenase(LDH), glutamic pyruvic transaminase (GPT) and glutamic oxaloacetic transaminase(GOT) were lower than those of the control, whereas magnesium($Mg^{++}$) and haemoglobin(Hb) were higher than the control. However total protein(Tp) and albumin (ALB) levels similar to the control. When the fish after giving a handling stress were cultured in NaCl-free water as a control, the frequency of gill congestion and the value of blood components showed a similar pattern to that obtained from the NaCl acclimated experiments. However, in the early period(1-3day) after handling stress, NaCl acclimating slightly helped the natural tendancy of recovery. These results suggest that the frequency of gill congestion is not always reduced over the whole period of NaCl acclimation. However, the acclimation to NaCl solution may be a somewhat useful therapy for the gill congestion in stressed eels. 뱀장어에 인위적으로 스트레스를 가하여 아가미 울혈증을 발생시킨 후에 NaCl 처리에 따른 CVS의 울혈증의 치료효과 및 혈액성상의 변화에 미치는 영향을 조사하였다. 스트레스 직후에 고빈도의 발병율을 보였던 아가미 및 간의 울혈증은 염처리 후 3일째 까지는 감소되었으나, 4일 이후에는 별 영향이 없었다. 혈액성분중 스트레스 후 증가되었던 Ht, Met-Hb, $Ca^{++}$, GOT, GPT, LDH 및 glucose치 등과 감소되었던 $Mg^{++}$ 및 Hb치 등은 염처리 3일까지는 회복율이 촉진되었으나, 그 이후에는 큰 차이를 보이지 않았다. 그러나 알부민 및 총단백치는 염처리 기간동안 별 변화가 없었다. 이상의 결과는 스트레스에 의해 발병된 아가미 울혈증에 대한 염처리법은 초기 3일까지는 효과가 있으나, 그 이상의 기간에는 전혀 효과적이지 못함을 보여준다.

예술심리치료사들의 미술작품창작 체험에 관한 연구 : 미술의 치료적 가치를 중심으로

최민순,박은선 한국예술심리치료학회 2019 예술심리치료연구 Vol.15 No.4

본 연구는 예술심리치료사들의 미술작품창작 체험을 통한 경험의 본질과 그 의미를 탐 색하는데 목적이 있다. 연구방법은 Manen의 해석학적 현상학 체험연구로 진행하였다. 본 연구의 참여자들은 예술심리치료임상경험이 있는 예술심리치료사 8명으로 주1회, 3시간씩 총10회기의 미술작품창작 작업에 참여하고 함께 전시회를 개최하는 체험을 하였다. 자료 수집은 작업기간 중에 집단으로 1회 2시간, 전시회까지 모든 체험이 끝난 후 개별적으로 90분씩 인터뷰를 통해 이루어졌고, 자료 분석을 거쳐 5개의 본질적주제와 15개의 하위주 제를 도출하였다. 본질적 주제는 <미술작품창작과정이 자기작업이 됨>, <미술창작능력의 향상이 치료사로서 자신감을 높여줌>, <임상 중에 만들어진 창작품을 더 소중하게 생각하 게 됨>, <미술창작 작업에서의 치유적 효과에 대한 이해가 깊어짐>, <동료와 함께 한 작 업이 힘이 됨>이었다. 이러한 본질적 주제를 통한 결론은 숙고하는 예술창작 과정은 그 자체로 치유적 특성을 가지고 있으며, 예술본연의 가치가 예술치료과정에 통합 될 때에 예 술심리치료와 예술심리치료사의 정체성이 보다 확고해 질 수 있다는 것을 다시금 확인하 게 되었다는 것이다. 또한 이러한 확신은 치료자로 하여금 임상 내에서 예술작업과 작품에 좀 더 소신을 가지고 적극적인 역할을 하도록 도왔다. The purpose of this study was to explore the nature and significance of the art creation experience by art therapists. For this purpose, Manen’s methodology of hermeneutical phenomenology was utilized. The participants of this study were eight art therapists with clinical experience. They participated in a total of ten artworks once a week for three hours each time, and then held exhibitions for the artwork produced. The data was collected from April 2018 to October 2018 and included one where an interview was conducted during the work period, followed by 2 hours of artwork in a group and participants were individually interview once for 90 minutes, then on completion of the exhibition. In this respect, the 5 essential themes and 15 sub-themes were derived based on the data. The essential themes were as follows: <Creating art works becomes a self-work>, <Improving artistic skills enhances confidence.>,<The art work created at the clinical session was more valuable.>, <There is a deeper understanding of the healing effect in work of creating art.>, <Participants felt supportive in their creative work with their peers.> The conclusions of this study were that it demonstrated that the artistic process of contemplation has its own healing qualities and the identity of arts therapy. It was shown that arts therapists can be more firmly established when the value of artistic creation is integrated into the art therapy process. Broadly speaking, this conviction has also helped therapists to take an active role in clinical practice, with more confidence in arts and artwork.

자기성찰을 중심으로 한 집단미술치료가 심리적 안녕감에 미치는 효과

최민순,박은선 한국예술심리치료학회 2017 예술심리치료연구 Vol.13 No.1

본 연구는 자기성찰을 중심으로 한 집단미술치료가 심리적 안녕감에 도움을 주는지를 밝히는 것을 목적으로 집단미술치료가 참가자의 자기성찰과 심리적 안녕감에 미치는 영향, 그리고 각각의 변화는 어떻게 나타났는지를 연구문제로 설정하였다. 자기성찰 프로그램은 총 15회기로 구성하였으며, 매주 1회로 2016년 5월 27일부터 2016년 9월 9일까지 진행되었 다. 연구대상은 인터넷 홍보를 통해 모집한 자발적인 참가자를 대상으로 실험집단과 통제 집단 각각 10명씩 무선 배정 하였다. 측정도구는 자기성찰 척도와 심리적 안녕감 척도를 사용하였고, 자료 분석에서 집단 간 동질성 검정을 위해서는 맨 휘트니 U 검정 (Mann-Whiteny U test)을, 자기성찰과 심리적 안녕감의 사전 사후 결과 비교를 위해서는 윌콕슨 순위 부호 검정(Wilcoxon’s singed rank test)을 사용하였다. 그 결과 실험집단은 자기성찰, 심리적 안녕감 모두 유의한 변화를 보인 반면 통제집단은 유의한 변화를 보이지 않아 자기성찰을 중심으로 한 집단미술치료 프로그램이 자기성찰과 심리적 안녕감 향상에 기여했다고 보았다. 또한 프로그램 진행과정 중에 나타난 자기성찰과 심리적 안녕감의 변 화 양상을 살펴보기 위해서 참여자들의 보고를 질적으로 분석, 제시하였다. This study is to investigate whether group art therapy adopting self-reflection helps improve psychological well-being. The group therapy consisted of 15 sessions focusing on their self-reflection. This program was carried out once a week from May, 27 to September, 9, 2016. Participants voluntarily applied for the program in the Internet PR. They were arranged into 2 groups(10 people in each group) at random. One group is experiment and the other is the control group. Mann-Whitney U test was employed for the analysis of pre-test scores, the comparison of self-reflection scales, and psychological well-being scales. For the post-test scores comparison, Wilcoxon's singed rank test was used. The participants' reports were also qualitatively analyzed in order to examine what changes they experienced in terms of self-reflection and psychological well-being during the program. The experimental group showed significant improvement in both self-reflection and psychological well-being whereas the control group did not show any significant change. In conclusion, it can be said that this group art therapy program contributed to improving self-reflection and psychological well-being of the participants.