Efficient Interleukin-21 Production by Optimization of Codon and Signal Peptide in Chinese Hamster Ovarian Cells

( Hee Jun Cho ),( Byung Moo Oh ),( Jong-tae Kim ),( Jeewon Lim ),( Sang Yoon Park ),( Yo Sep Hwang ),( Kyoung Eun Baek ),( Bo-yeon Kim ),( Inpyo Choi ),( Hee Gu Lee ) 한국미생물 · 생명공학회 2019 Journal of microbiology and biotechnology Vol.29 No.2

Interleukin-21 is a common γ-chain cytokine that controls the immune responses of B cells, T cells, and natural killer cells. Targeting IL-21 to strengthen the immune system is promising for the development of vaccines as well as anti-infection and anti-tumor therapies. However, the practical application of IL-21 is limited by the high production cost. In this study, we improved IL-21 production by codon optimization and selection of appropriate signal peptide in CHO-K1 cells. Codon-optimized or non-optimized human IL-21 was stably transfected into CHO-K1 cells. IL-21 expression was 10-fold higher for codon-optimized than non-optimized IL-21. We fused five different signal peptides to codon-optimized mature IL-21 and evaluated their effect on IL-21 production. The best result (a 3-fold increase) was obtained using a signal peptide derived from human azurocidin. Furthermore, codon-optimized IL-21 containing the azurocidin signal peptide promoted IFN-γ secretion and STAT3 phosphorylation in NK-92 cells similar to codon-optimized IL-21 containing original signal peptide. Collectively, these results indicate that codon optimization and azurocidin signal peptides provide an efficient approach for the high-level production of IL-21 as a biopharmaceutical.

만성 승모판 패쇄부전증 환자에서의 안지오텐신 전환효소 억제제의 장기 투여 효과

김대희,이명묵,이해영,조현재,박승정,서재빈,서정원,양한모,윤창환,조상호,이준희,김용진,김명아,손대원,오병희,박영배 대한순환기학회 2004 Korean Circulation Journal Vol.34 No.2

다발성 대장암 간전이 환자의 간절제를 위한 새로운 시도

주종우,김형철,임철완,신응진,조규석,유기원,송옥평,홍대식,박성진,조준희,이혜경,김희경,권계원,고은석 순천향의학연구소;Soonchunhyang Medical Research Institute 2004 Journal of Soonchunhyang Medical Science Vol.10 No.2

Multiple bilobar liver matastases (MBLM) are the main cause of low resectability in the colon cancer liver metastases. The authors experienced one case of initially non-resectable colon cancer liver metastases. He was curatively and safely treated with a two-stage hepatectomy using the new method of future remnant liver volume growing. A 54-year-old man was referred to our department with the sigmoid colon cancer combined with MBLM, which were checked in two small metastatic lesions in the left lobe and five large sized lesions in the right lobe in the computed tomogram (CT). A laparoscopic assisted anterior resection was primarily performed. We performed the 1^(st) stage hepatectomy 3 weeks after the colon resection. Intra-operative Ultrasonogram (US) found 9 small superficial metastatic lesions in the left lobe. All that lesions were completely removed by non-anatomical wedge resection. An occlusion ballon catheter was placed in the right portal vein through a small branch of the inferior mesenteric vein at that time. The future remnant liver volume was sufficiently increased 3 weeks after the 1^(st) hepatectomy. A right hepatectomy was safely performed 22 days after the 1^(st) hepatectomy. The patient received a regional chemotherapy (interleukin2 based immuno-chemotherapy through hepatic artery) for 4 months, then received 9 cycles of systemic chemotherapy (biweekly Oxaliplation, leucovorin, plus 5-fluorouracil) without any recurrence evidence.

면역억제제를 사용치 않은 동소성 간이식 실험견의 조직병리소견

조원현,최순옥,김인호,임태진,김성수,박준희,김우석,김현철,최규택,김진모,권건영 大韓移植學會 1994 Korean Journal of Transplantation Vol.8 No.1

용융염산화 반응기에서 기체체류량 및 기-액 흐름특성

조용준,양희철,은희철,유재형,김준형 한국화학공학회 2003 Korean Chemical Engineering Research(HWAHAK KONGHA Vol.41 No.5

용융염산화는 혼성폐기물과 유해폐기물을 효과적으로 산화 및 분해할 수 있는 소각대체기술중 하나이다. 본 연구에서는 직경이 0.079 m이고 높이가 0.653 m인 용융염산화 반응기에서 기체체류량 및 기-액 흐름특성에 대한 연구를 수행하였다. 액상으로 용융탄산나트륨을 기상으로 건조된 공기를 사용하였으며 기체유속(0.05-0.22 m/s)과 용융염온도(870-970℃) 변화가 기체체류량 및 기-액 흐름특성에 미치는 영향을 규명하였다. 기체체류량은 용융염 온도가 상승함에 따라 증가하였는데, 이는 용융염 온도의 증가로 인해 용융된 탄산나트륨의 점도와 표면장력이 감소하였기 때문이다. 실험에서 얻어진 기체체류량 데이터를 drift-flux 모델에 적용하여 용융염반응기 내의 흐름특성을 규명할 수 있었으며, 이를 통해 흐름영역에 따른 기체체류량을 정확하게 예측할 수 있었다. Molten salt oxidation is one of the most promising alternatives to incineration that can be used to efficiently destroy the organic components of mixed wastes and hazardous wastes. In this study, the gas holdup and gas-liquid flow characteristics are investigated in the molten salt oxidation reactor (0.076 m D×0.653 m H.). Effects of input air velocity (0.05-0.22m/s ) and molten salt temperature (870-970℃) on the gas holdup and flow characteristics have been studied. Molten carbonate as the liquid phase and air as the gas phase have been used in this study. The gas holdup increases with increasing molten salt temperature due to the decrease of viscosity and surface tension of molten carbonate. The experimentally obtained gas holdups in the molten salt reactor have been well described and characterized by means of drift-flux model. The gas holdups with variation of the flow regime have been well predicted.

효율적인 인터넷 빌링서비스 발전방향 및 EBPP시스템에 관한 연구

趙元熙,李俊錫 동남보건대학 2001 論文集-東南保健大學 Vol.19 No.2

Though Internet Billing Service Market has been paid much attention to, it has not developed so rapidly as many experts expected. Current situation of B2C market reminds us of traditional "chicken and egg problem." That means billers are reluctant to accept On-line billing until many core consumers willingly pay bills on line. On the other hand, customers are hesitating to pay using on-line billing until a lot more bills are possibly processed on line. Last rear due to the venture fever, many of domestic Internet Billing Service companies were established and started services, but the services has not been expanded and boomed as much as ordinary customers can feel and the profit basis structure is still weak. Some experts say that it is difficult to anticipate high selling and Front only with Internet Billing Services . In this study these problems of Internet Billing market are investigated and service development directions , in order to got high selling by inducing customers of Internet Billing Services and generating various commerce activities, are searched.

주의력결핍과잉행동장애와 Alpha-1C-Adrenergic 수용체 유전자와의 연관성에 대한 연구

조수철,김재원,김붕년,황준원,박미라,김순애,조대연,유희정,정운선,손정우,박태원 大韓神經精神醫學會 2008 신경정신의학 Vol.47 No.1

Objectives : Neurobiological and pharmacological research has suggested that the dysregulation of the central noradrenergic systems might be involved in the pathophysiology of ADHD. The aim of this study was to examine the association of the alpha-1C-adrenergic receptor gene (ADRA1C) with ADHD in Korean subjects. Methods : In a case-control study, we assessed 186 DSM-IV ADHD probands and 150 normal controls. One hundred and eight trios were studied in a family-based association analysis. Psychiatric diagnoses were derived through structured diagnostic interviews. For the clinical evaluation of the ADHD subjects, the Child Behavior Checklist (CBCL), the ADHD Rating Scale-IV (ARS) and the Junior Temperament and Character Inventory (JTCI) were administered. A computerized continuous performance test (CPT) was used to measure the inattention and impulsivity of the ADHD children. Results : There were no significant differences in the genotype or allele frequencies of the ADRA1C PstI polymorphism between the ADHD and control group (p>0.05). The transmission disequilibrium test (TDT) analysis observed no evidence for biased transmission of any of the alleles of the PstI polymorphism. There were no significant differences in the CPT or JTCI profiles between those ADHD subjects with the CC genotype and those with the other (CT+TT) genotypes at the PstI polymorphism. Conclusion : The results of this study do not support the ADRA1C as a major genetic susceptibility factor in ADHD.

K3에 축적된 내부 응력이 피로 파절에 미치는 영향

김준영,김진우,조경모,박세희 大韓齒科保存學會 2012 Restorative Dentistry & Endodontics Vol.37 No.2

Objectives: This study aimed to evaluate the relationship between the cyclic fatigue of a K3 file and internal stress intentionally induced until the activation of the auto-stop function of the torque-controlled motor. Materials and Methods: K3 (Sybron Endo) .04 and .06 taper, size 25, 30, 35, 40 and 45 were used in this study. To give the internal stress, the K3 files were put into the .02 taper Endo-Training-Bloc (Dentsply Maillefer until the activation of the auto-stop function of the torque-controlled motor. The rotation speed was 300 rpm and torque value was 1.0 N·cm. K3 were grouped by the number of induced internal stress and randomly distributed to 4 experimental groups (n = 10, Stress 0 [control], Stress 1, Stress 2 and Stress 3). For measuring the cyclic fatigue failure, the K3 files were worked against a sloped glass block and time for file separation was recorded. Data was statistically analyzed Statistical analyses were performed using two-way ANOVA and Duncan post-hoc test at p < 0.05 level. Results: Except .04 taper size 30 in Stress 1 group, there were statistically significant differences in time for file separation between control and all experimental groups. K3 with .04 taper showed higher cyclic fatigue resistance than those of .06 taper. Conclusion: In the limitation of this study, the cyclic fatigue of the K3 file was influenced by the accumulated internal stress from use until the auto-stop function was activated by the torque-controlled motor. Therefore, clinicians should avoid the reuse of the K3 file that has undergone auto-stops. (Restor Dent Endod 2012;37(2):74-78)

요근 농양이 동반된 Salmonella typhi 척추골수염 1예

조윤행,홍성수,김은옥,이미숙,김양수,우준희,배직현,류지소 대한화학요법학회 1999 대한화학요법학회지 Vol.17 No.2

저자들은 아직 국내에 보고된 적이 없는 S. typhi에 의한 척추골수염 환자에서 ciprofloxacin을 5주간 투여하여 임상적 호전을 보였던 증례를 경험하였기에 보고하는 바이다. Vertebral osteomyelitis by Salmonella infection is rare. Although it is known to be associated with sickle cell anemia, various hemoglobinopathies and immunosuppressive states, it may also occur in normal host. To date, vertebral osteomyelitis with Salmonella. typhi has not been reported in Korea. We experienced a case of spinal osteomyelitis due to S. typhi in a 48-year old man who was admitted because of low back pain for two months. MRI scan showed vertebral osteomyelitis in L1 & L2. Needle aspiration was performed by CT guidance. S. typhi was identified from aspirated pus. However, any organism was not identified from blood and urine cultures. He was treated with ciprofloxacin for 5 weeks successfully.

운동선수의 체간부 등속성 근기능 및 유연성과 요통과의 관계

전종귀,박희근,송영민,조병준 충남대학교 체육과학연구소 2000 體育科學硏究誌 Vol.18 No.1

The relationships of the isokinetic muscle functions and the flexibility of body trunk to low back pain(LBP) were investigated in 16 female ahtletes aged 21.5 years. Some of them were suffering from LBP syndrome without clinical signs of lumbosacral nerve root irritation or compression. The isokinetic muscle functions of subject's body trunk were evaluated using Cybex770. The peak torques of trunk extensors and flexors were measured at 60˚ /sec, respectively. The powers and the total works of trunk extensors and flexors were measured at 180˚ /sec, respectively. The back strength and the trunk flexibility of the subjects were measured with dynamometer and flexormeter. The frequency scale, the visual analogue scale(VAS), and the verbal rating scale(VRS) of subject's LBP were evaluated by interview. The Pearson' Rs between the variables and their significances were calculated for statistical analysis. The coclusions were as follows. 1.The frequency of LBP in female athletes was once in four months. 2.The VAS(0-10) of LBP in female athletes was 2.62. 3.The VRS(1-7) of LBP in female athletes was 2.81(weak). 4.The significant negative correlations were observed between the frequency of LBP and the muscle mass, the trunk flexibility, and back strength of subject. 5.The weak correlations exist between the VAS of LBP and the age of subject, between the VRS of LBP and the trunk flexibility of subject. 6.There were not any significant correlations between the variables of LBP and the isokinetic muscle functions, and other physical and physiological variables.