Effects of <i>Ginkgo biloba</i> Extract on Promotion of Neurogenesis in the Hippocampal Dentate Gyrus in C57BL/6 Mice

YOO, Dae Young,NAM, YoonYi,KIM, Woosuk,YOO, Ki-Yeon,PARK, Jaeil,LEE, Choong Hyun,CHOI, Jung Hoon,YOON, Yeo Sung,KIM, Dong-Woo,WON, Moo-Ho,HWANG, In Koo Japanese Society of Veterinary Science 2011 The Journal of veterinary medical science Vol.73 No.1

<P><I>Ginkgo biloba </I>leaf extract (Gb) has been known to improve blood flow and preclude the tissue from free radical damage. Effects of Gb were examined by using Ki67, a specific proliferative marker for cellular proliferation, and doublecortin (DCX), a marker for immature neurons, indicating degree of neuroblast differentiation in the hippocampal dentate gyrus (DG) of adult C57BL/6 mice. The mice were fed with Gb at 40 and 100 mg/kg once daily for 28 days. The increase of Ki67- and DCX-immunoreactive cells in the DG was increased in a dose-dependent manner. Especially, the group having 100 mg/kg Gb showed a significant increase of DCX-immunoreactive neuroblasts with well-developed tertiary dendrites. Expression of DCX protein in the Gb groups was also significantly increased upon compared with the vehicle group. The results suggested that repeated intake of Gb would enhance cell proliferation and neuroblast differentiation in the mouse DG.</P>

Effects of pyridoxine on a high‐fat diet‐induced reduction of cell proliferation and neuroblast differentiation depend on cyclic adenosine monophosphate response element binding protein in the mouse dentate gyrus

Yoo, Dae Young,Kim, Woosuk,Yoo, Ki‐,Yeon,Nam, Sung Min,Chung, Jin Young,Yoon, Yeo Sung,Won, Moo‐,Ho,Hwang, In Koo Wiley Subscription Services, Inc., A Wiley Company 2012 JOURNAL OF NEUROSCIENCE RESEARCH - Vol.90 No.8

<P><B>Abstract</B></P><P>In this study, we challenged pyridoxine to mice fed a high‐fat diet (HFD) and investigated the effects of pyridoxine on HFD‐induced phenotypes such as blood glucose, reduction of cell proliferation and neuroblast differentiation in the dentate gyrus using Ki67 and doublecortin (DCX), respectively. Mice were fed a commercially available low‐fat diet (LFD) as control diet or HFD (60% fat) for 8 weeks. After 5 weeks of LFD or HFD treatment, 350 mg/kg pyridoxine was administered for 3 weeks. The administration of pyridoxine significantly decreased body weight in the HFD‐treated group. In addition, there were no significant differences in hepatic histology and pancreatic insulin‐immunoreactive (‐ir) and glucagon‐ir cells of the HFD‐treated group after pyridoxine treatment. In the HFD‐fed group, Ki67‐positive nuclei and DCX‐ir neuroblasts were significantly decreased in the dentate gyrus compared with those in the LFD‐fed mice. However, the administration of pyridoxine significantly increased Ki67‐positive nuclei and DCX‐ir neuroblasts in the dentate gyrus in both LFD‐ and HFD‐fed mice. In addition, the administration of pyridoxine significantly increased the protein levels of glutamic acid decarboxylase 67 (GAD67) and brain‐derived neurotrophic factor (BDNF) and the immunoreactivity of phosphorylated cyclic AMP response element binding protein (pCREB) compared with the vehicle‐treated LFD‐ and HFD‐fed mice. In contrast, the administration of pyridoxine significantly decreased HFD‐induced malondialdehyde (MDA) levels in the hippocampus. These results showed that pyridoxine supplement reduced the HFD‐induced reduction of cell proliferation and neuroblast differentiation in the dentate gyrus via controlling the levels of GAD67, pCREB, BDNF, and MDA. © 2012 Wiley Periodicals, Inc.</P>

마우스 종양발생에서 Nitric Oxide의 역할에 관한 연구 Ⅲ : Helicobacter pylori에 의해 유발된 마우스 위암에서 염증매개인자의 역할 The role of inducible nitric oxide synthase and cyclooxygenase-2 in H. Pylori-associated gastric carcinogenesis

남기택,오상연,조현무,이국경,강진석,제정환,최미나,한상욱,김대용,장동덕,양기화,안병우 식품의약품안전청 2001 식품의약품안전청 연보 Vol.5 No.-

feficotorfer fyf☞ri(Hp)가 위암파 관련이 있다는 역학적인 증거는 많이 있지만 이에 대한 정확한 기전에 대해선 밝혀져 있지 않고 있으며 실험동물 모델도 적절하지 못한 것으로 알려져있다. 본 실험에서는 위암의 원인으로 알려져 있는 f, fyforf'(Hp)를 이용하여 마우스에서 위암 모델을 확립하고 만성염증과정 중에 생성되는 리0와 COX-2 등의 발현이 위암발생에 미치는 명향을 통하여 예방과 치료를 위한 점근을 시도하고자 하였다. 마우스를 7군으로 나누어서 1, 2, 3, 4군의 등물은 MNU를 증류수에 200ppm 농도로 음수병득 이용하띤 10주간 격주로 투여하였으며 MHU 음술 투여 1주 휴씩 후 배양한 f. fyrofi 를 약 109cru/rfll 로 맞춰 한 마리당 0.1ml 씩 이틀 간격으로 세 번에 걸쳐 하룻방 금식시킨 1, 2, 3, 5, 6, 7군기 마우스의 위장에 투입하떴다. 균 투입을 마친 후 다응 날부터 2군쏙 6군은 iNOS 억제제인 aminoguanidine(AG)을 음수병으로 툰여하였으며 3군과 7군은 COX-2 척제제인 nimesulide(NSD)를 투여하였다. 위의 종양발생양상을 샅최보면 bfNU와 Hp만을 투여한 1 관 ; (hfNU +Hp), 2군 : iNO을 inhibitor 투여군(MNU+HP+AG'1. 3군 ;CO딘-2 Inilibitor 투여군(MNU누Hp누 NSD), 4군 ,MNlf 단독투여군, 5군 ;Hp 단독투여춘, 6군 ; 러p 단독에 AG투여군, 7군 , Hp 단독에 NSD투여군의 종양발샐을은 각각 쁜.Bff(l1/16), 70.6%f12/ti), 했.9ff(7/18), 10%(1/10), Off(O/IS)0%(O/S), 0%(O/5)의 발쟁율을 보여 iNOS 억제제인 AC은 좁양발생을 억계하지 못하였으며, COX-2억제제인 NSD 는 종양발생을 유의적으로 감소시켰다. 콩양발생개수에서는 2.62±0.36, 1.41츠0.14, 0.44 르0.12, 0.10±0.10을 보여 AC와 NSD에서 유의성 있게 발생개수를 줄였다. Hp 단독투여에 의해즌는 종양발생이 나타나지 않았으며 HP+AG, Hp+ IfSD 추여군에콕 시험증료 시점에 약물에 의해 Hp의 제균효과가 있는지의 여부를 확인끓기 위하여 PCR을 이용하여 확인한 결과 모두 양성인 것으로 나타나 Hp의 제균효과에 의한 촐양발생 억제가 일어나지는 않았다. 위의 결과로 볼 때 Hp는 위암발생을 촉진하는 것으로 나타났고 딘p 감염시 매개되는 염증인자를 억제하였을 때 종양발생을 억제하는 것으로 위암 발생에서 염증매개인자는 종양을 촉진하는 것으로 나타났으며 it,705 억제제쓱 COX-』 억제제의 위알 예밭효과fl는 효과적일 것으로 사료된다 In spite of a large volume of epidemiological evidence indicating significant relationship between H. pylori infection and gastric adenocarcinoma, a doubt still exists on an elevated risk of stomach cancer by H. pylori infection due to lack of direct evidence of their exact mechanistic link. It is, therefore, essential to have an appropriate animal model for detailed analysis of the role of H. pylori played in gastric carcinogenesis. There is a wealth of evidence to support that over production of inducible nitric oxide synthase(iNOS) and cyclooxygenase-2(COX-2) is involved in the pathogensis of various cancer in both rodents and humans. The aim of this study was to establish a mouse model for H. pylori-associated gastric carcinogenesis and to identify the role of inducible nitric oxide synthase(iNOS) and cyclooxygenase-2(COX-2) played during the gastric carcinogenesis in mice. Eighty-three specific pathogen free, six-week-old male C57BL/6 mice were randomly divided into seven groups. Animals of the group 1, 2, 3, 4 were given MNU in their drinking water at the concentration of 200 p.p.m. for total five cycles of one-week regimen with one-week pause. After completion of MNU administration, they were given autoclaved distilled water for one weeks, and groups 1, 2, 3, 5, 6, 7 were inoculated with H. pylori. After completion of H. pylori. inoculation, groups 2 and 6 were given aminoguanidine in their drinking water at concentration of 1000p.p.m. and animals of group 3 and 7 were given the diet containing 200 ppm nimesulide at 12 weeks of age. All animals were killed at 50 weeks of age. The incidences of the glandular stomach tumors in the group 1, 2, 3 and 4 were 87.5%(14/16), 76.4%(13/17), 44.4(8/18), 10.0%(1/10), respectively and the tumor incidence of group 3(MNU→Hp+nimesulide) was significantly lower than those of group 1(MNU→Hp) at the value of P<0.01. The average numbers of tumors of group 2(MNU→Hp+AG : 1.41±0.24) and group 3(MNU→Hp+nimesulide : 0.44±0.12) were significantly lower than those of group 1(MNU→Hp : 2.62±0.36) at the value of P<0.05. Therefore, overproduced iNOS and COX-2 plays an important role in mice gastric carcinogenesis. We concluded iNOS and COX-2 inhibitor have good effects on gastric carcinogenesis.

조혈모세포이식환자에서 조혈기능의 회복과 관련된 인자들에 관한 연구

정기주,강명수,권기두,김경하,이상철,김현정,배상병,김찬규,이남수,이규택,원종호,홍대식,박희숙 순천향대학교 의학연구소 2007 Journal of Soonchunhyang Medical Science Vol.13 No.1

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불소가 첨가된 실리콘 산화막의 다층금속절연 특성에 관한 연구

權大赫,南基泓,吳相光 慶一大學校 1997 論文集 Vol.14 No.2

The characteristics of fluorinated silicon oxide (SiOF) films prepared by plasma enhanced chemical vapor deposition(PECVD) were discussed. The deposition of these films was carried out in the temperature range of 80 - 220℃ in a conventional parallel plate plasma reactor with 〈 ±3% uniformity by flowing 2 sccm of disilane (Si₂H6), 100 sccm of nitrous oxide (N₂O), and 20 sccm of tetrafluoromethane (CF₄). AS the deposition temperature increased from 80 to 220℃, the deposition rate of the films increased from 16.7 to 18.4 nm/min; meanwhile, the etch rate decreased from 2.69 to 1.48 nm/sec. The refractive index was 1.46 regardless of the deposition temperature. The Fourier transform infrared (FTIR) spectra of the films showed decreasing Si-O stretching wave number with increasing full width at half maximum (FWHM) as deposition temperature increased. The high frequency capacitance-voltage (C-V) measurements of the metal-oxide-semiconductor (MOS) capacitors fabricated using the films showed increasing effective oxide charge density and decreasing dielectric constant with increasing deposition temperature. Deposition temperature of 180℃ resulted in films with the smallest dielectric constant of 3.75. These films showed an average breakdown strength of 9.14 MV/cm with 77.5% of the MOS capacitors having breakdown field strength ≥9.5 MV/cm

지문 정합에 관한 연구

朴一男,金栢起,郭允植,李大寧 慶熙大學校 1991 論文集 Vol.20 No.-

In this paper, a fingerprint recognition system for identification and classification of fingerprint is described. To develop an efficient fingerprint identification system, modified algorithms are proposed in this thesis. The recognition algorithm consists of preprocessing(acquisition, binarization, thinning, restoration), classification and matching. Experimental thinning show that identification rate is about 91% for 80 file fingerprints and classification rate is more than 98% for 80 input fingerprints.

하악골 후방이동시 중안면부 연조직의 변화양상에 대한 임상통계학적 연구

한대희,김수남,민승기,김태성,성헌모 대한악안면성형재건외과학회 2000 Maxillofacial Plastic Reconstructive Surgery Vol.22 No.3

Prediction of the soft tissue changes following hard tissue movement is very important from the esthetic view point for patients who have orthognathic surgery. There are many cephalometric analysis of facial bone and soft tissue on the lower lip and chin region but few soft tissue analysis on the midface after mandibular setback surgery. This study was performed to obtain whether the mandibular posterior movement has influence on the midface and the predictable ratio of post-operative measurement values of the soft tissue changes following mandibular setback surgery. Fifteen patients (8 males and 7 females) who had undergone mandibular setback surgery were selected and analyzed the soft tissue movement on the upper lip and the cheek region. Post-operative changes of the soft tissue measurements after mandibular surgery were examined on pre- and post-operative cephalo-metrics and the ratio of changes were analyzed after drawing the reference line on the face with the barium sulfate solution. The reference lines were perpendicular to the intercanthal line from infraorbital foramen and lateral canthus. The results obtained were as follows : 1.There were tendancy of anterior movement of soft tissue adjacent the nose after mandibular surgery 2.There were incerased tendancy of the amount of anterior movement from the nasal crease to the cheek region. 3.The amount of anterior movement of the soft tissue was larger below the palatal plane compared with above the palatal plane in the cheek region. 4.The upper lip length was increased and moved posterior direction after mandibular setback surgery 5.The lower lip was moved posterior direction by posterior movement of the mandibular structure 6.Soft tissue of the midface around the nose moved anterior direction after mandibular setback surgery but there was no correlation between the amount of mandibular setback and the amount of the soft tissue changes

관동맥 재개통 후 QTc간격분산의 감소 : 정량적으로 측정한 심근 허혈의 호전 정도와의 연관성 Relationships with the Quantitative Degree of Improvement in Myocardial Ischemia

박상선,최기준,남기병,이철환,홍멸기,박성욱,박승정,신중우,문대혁,김유호 대한순환기학회 2000 Korean Circulation Journal Vol.30 No.8

새로운 조성을 갖는 고형 탈묵제의 제조

최형철,남기대,정노희 한국공업화학회 2003 공업화학 Vol.14 No.3

펄프섬유로부터 잉크입자를 효율적으로 제거하여 잉크함량이 적고 백색도가 높은 재생펄프용 탈묵제를 제조하였다 본 연구의 또 다른 목적은 고백색도에 필요한 여러 계면활성제의 적정조건을 찾고 재생펄프의 수율을 높이는 것이다. 본 연구에서 우리는 우수한 탈묵효과를 위해 스테아르산과 여러 유형의 계면활성제를 사용하였다. 그리고 탈묵효율에 미치는 여러 가지 고형 탈묵제의 효과를 알기 위하여 부상탈묵실험을 수행하였다. 그 결과 고백색도와 높은 수율을 얻을 수 있는 새로운 고형 탈묵제의 적절한 조합조성은 스테아르산 75%와 폴리옥시알킬렌알킬아릴에테르 6%를 혼합한 것이었다. 또한 탈묵효과를 폭넓게 적용하기 위해 기포력과 피 안정도, 분산능을 테스트하였다. 새로운 타입의 탈묵제는 폐지 탈묵시 우수한 분산능과 적당한 기포력과 안정성을 나타내었다. A new feinting agent for recycling of waste paper was produced. This agent efficiently removed ink particles from the pulp fiber and the resulting recycled pulp had a high brightness and a low content of residual ink. Efforts were made to find an optimum condition of various surfactants used for high brightness and yield of recycled pulp. In this study, stearic acid was used for various types of general surfactants for deinking. Flotation deinking experiments were conducted in order to examine the effect of various solid-type deinking agents. As a restult, an optimum combination of this new solid-type deinking agent, which resulted in high brightness and yield, was 75 wt% stearic acid mixed with 6wt% polyoxyalkylene alkyl aryl ester. This new type deinking agent had good dispersability, suitable foaming power and foam stability in deinking of waste paper.

김대건 (안드레아) 신부 얼굴복원

한승호(Seung-Ho Han),박대균(Dae-Kyoon Park),이우영(U-Young Lee),고기석(Ki-Seok Kohl),김희진(Hee-Jin Kim),허경석(Kyung-Seok Hu),남용석(Yong-Seok Nam) 대한체질인류학회 2001 대한체질인류학회지 Vol.14 No.3

본 작업은 서울대교구 명동성당에서 가툴릭의대에 우리나라 최초의 신부인 김대건 신부의 얼굴복원작 업을 의뢰해 1999년 9월부터 약15개월에 걸쳐 북원작업을 한 것으로 1971년 가툴릭의대 해부학교실에서 조사한 김대건 신부 머리뼈의 직접제측치와 사진 자료를 창조하여 제작하였다 작업의 과정은 머리뼈 계측값이 가장 비슷한 머리뼈를 찾아 복제를 하고 계측치에 맞게 수정한 후, 해부학 실습용 시체에서 직접계측하거나 초음파를 이용하여 간접계측을 한 한국인 얼굴의 표준두께를 적용하여 영굴을 복원하였다. 아울러 한국인에서 조사 한 머리뼈와 얼굴 각 부위의 연관관계자료를 토대로 유토를 이용한 복원작업을 시행하였으며 최종 결과물은 청동을 소재로 흉상을 만들었다. 이와 같은 작업은 법의학분야에서 신원미확인 머리뼈가 발견되었을 때 얼굴복원을 통해 신원을 확인하는 법 의인류학적 연구의 하나로, 한국인의 자료를 토대로 작업한 최초의 영굴복원이며 앞으로 신원확인을 위해 얼굴 을 복원하는 작업에 도움이 될 것으로 생각한다.