http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
A High Fiber Diet Responsive Case in a Poodle Dog with Long-Term Plant Eating Behavior
KANG, Byeong-Teck,JUNG, Dong-In,YOO, Jong-Hyun,PARK, Chul,WOO, Eung-Je,PARK, Hee-Myung Japanese Society of Veterinary Science 2007 The Journal of veterinary medical science Vol.69 No.7
<P>An 11-year-old, castrated male Miniature Poodle dog was referred due to daily plant eating followed by vomiting for 7 years. Each time the dog went out for a walk, he had been munched grass, and then vomited. There were no abnormal findings on medical examinations. Through behavior examination and consultation, we diagnosed this dog as having a plant eating problem. Because we assumed that the dog had eaten plants to supply of dietary deficiency or relieve digestive problems, advised the owner to discontinue supplying previous diet and to feed high-fiber diet. On the follow up, the owner said that the dog had not eaten plants and vomited from 3 days after initial dietary change. The dog has not shown any clinical signs for 13 months. This clinical finding shows a possibility that deficient diet, especially fiber, relates to the plant eating behavior of a dog.</P>
Proteomic Analysis of Focal Cerebral Ischemic Injury in Male Rats
Japanese Society of Veterinary Science 2010 The Journal of veterinary medical science Vol.72 No.2
<P>The present study identified the proteins that are differentially expressed during ischemic brain injury. Adult male rats were performed a middle cerebral artery occlusion (MCAO) to induce cerebral ischemia, and brains were collected at 24 hr after MCAO. Protein analysis was performed on the cerebral cortex using two-dimensional gel electrophoresis. Protein spots with a greater than 3 fold change in intensity between the sham and MCAO groups were identified by mass spectrometry. Among these proteins, 60 kDa heat shock protein, dehydropyrimidinase-related protein 2, t-complex protein 1, and Rho GDP dissociation inhibitor levels were significantly increased in MCAO group compared to those of the sham group. In contrast, thioredoxin, peroxiredoxin-2, stathmin, ubiquitin carboxy-terminal hydrolase L1, guanine nucleotide-binding protein α, pyridoxal-5'-phosphate phosphatase, and apoplipoprotein A-I levels were significantly decreased in MCAO group. These results suggest that cerebral ischemia induces neuronal cells death by changing expression levels of several proteins.</P>
LEE, Hyun,BAE, Jae-Sung,JIN, Hee Kyung Japanese Society of Veterinary Science 2010 The Journal of veterinary medical science Vol.72 No.6
<P>Niemann-Pick type C (NP-C) disease is a devastating developmental disorder with progressive and fatal neurodegeneration. We have used a mouse model of Niemann-Pick type C (NP-C) disease to evaluate the effects of direct intracerebral transplantation of human umbilical cord blood-derived mesenchymal stem cells (hUCB-MSCs) on the progression of neurological disease in this order. Here, we show that hUCB-MSCs transplantation into NP-C mice prevents the loss of Purkinje neurons and inhibits cerebellar apoptotic cell death. Interestingly, these effects were associated with the modulation of inflammatory responses, as evidenced by increased anti-inflammatory cytokine IL-10, and reduced abnormal astrocytic activation. Furthermore, our results show that the hUCB-MSCs transplantation reduced the cholesterol accumulation level in neurons in NP-C mice compared with sham-transplanted animals. This study provides the first evidence that hUCB-MSCs can improve neurological symptoms in NP-C disease, suggesting it as a potential therapeutic agent against neurodegenerative diseases.</P>
LEE, Ji Min,BAE, Jae-Sung,JIN, Hee Kyung Japanese Society of Veterinary Science 2010 The Journal of veterinary medical science Vol.72 No.8
<P>Recent studies have shown that many kinds of stem cells are beneficial for patients suffering with neurodegenerative diseases. We investigated the effects of neural stem cell (NSC), Maudsley hippocampal clone 36 (MHP36) in the Niemann-Pick disease type C (NP-C) model mice. Herein, we demonstrate that MHP36 transplantation improves the neuropathological features without acute immune response and promotes neuronal networks with functional synaptic transmission. The number of surviving Purkinje neurons substantially increased in MHP36 transplanted NP-C mice compared with sham-transplanted NP-C mice. MHP36 significantly reduced both of astrocytic and microglial activations. We also found that these surviving Purkinje neurons have normal functional synapses with parallel fibers that have normal glutamate release probability in MHP36 transplanted NP-C mice. Furthermore, real-time PCR analysis revealed up-regulation of genes involved in both excitatory and inhibitory neurotransmission encoding subunits of the ionotropic glutamate receptors GluR2, 3 and GABA<SUB>A</SUB> receptor β2. These findings suggest that NSC, MHP36 transplantation may have therapeutic effects in the treatment of NP-C and other neurodegenerative diseases.</P>
Species-Difference of Cyclooxygenase-2 in the Hippocampus of Rodents
KIM, Sung Koo,CHOI, Ji Won,HWANG, In Koo,YOO, Dae Young,YOO, Ki-Yeon,LEE, Choong Hyun,CHOI, Jung Hoon,SHIN, Hyung-Cheul,SEONG, Je Kyung,YOON, Yeo Sung,WON, Moo-Ho Japanese Society of Veterinary Science 2010 The Journal of veterinary medical science Vol.72 No.9
<P>Cyclooxygenase (COX) generates free radicals and it is important in inflammatory response. In this study, we observed the immunoreactivity in mice (ICR and C57BL/6 strain), rats and gerbils. In these animals, COX-2 immunoreactivity was mainly detected in pyramidal cells of the hippocampal CA2/3 region and in granule cells of the dentate gyrus. COX-2 immunoreactivity in the CA2/3 region was the highest in ICR mice, while in gerbils COX-2 immunoreactivity was the lowest; COX-2 immunoreactivity in the dentate gyrus was the highest in rats and the lowest in gerbils. The protein levels of COX-2 were similar to the immunohistochemical data. COX-2 mRNA transcript was the highest in the gerbil and the lowest in the rat. In brief, COX-2 protein, not mRNA, in the hippocampus is generally higher in mice (ICR and C57BL/6 strain) than rats and gerbils.</P>
Metallic Foreign Body in the Liver of a Dog
CHOI, Hojung,LEE, Youngwon,WANG, Jiwhan,YEON, Seongchan,LEE, Hyojong,LEE, Heechun Japanese Society of Veterinary Science 2010 The Journal of veterinary medical science Vol.72 No.11
<P>A 4-year-old beagle had intermittent vomiting and weight loss for 1 month. On plain radiography, an approximately 3.5-cm, radiopaque, linear foreign body was identified in the region of the liver. On ultrasonography, a hyperechoic linear structure with reverberation was identified in the left lobe or quadrate lobe. On computed tomography(CT), a thin hyperattenuating material consistent with a metallic foreign body was identified just medial to the gall bladder. Removal of the foreign body was performed without complications. We present a rare case in which a metallic foreign body was embedded in the liver asymptomatically and found incidentally during a clinical examination performed to ascertain the cause of clinical signs.</P>
Prevalence and Cross-Immunity of <i>Eimeria</i> Species on Korean Chicken Farms
LEE, Byung H.,KIM, Woo H.,JEONG, Jipseol,YOO, Jeongmi,KWON, Yong-Kuk,JUNG, Byeong Y.,KWON, Jun H.,LILLEHOJ, Hyun S.,MIN, Wongi Japanese Society of Veterinary Science 2010 The Journal of veterinary medical science Vol.72 No.8
<P>Epidemiology of <I>Eimeria</I> species in poultry flocks is important to increase the effectiveness of vaccinations and prophylactic strategies on chicken farms. In this study, fecal samples from 356 chicken farms were collected randomly and examined for the prevalence of <I>Eimeria</I> species. Through microscopic examination, it was determined that 78.7% of the tested farms were positive in <I>Eimeria</I>-infection. Seven <I>Eimeria</I> species were detected in all the positive farms by PCR amplification of the internal transcribed spacer 1 (ITS-1) region with species-specific primers. <I>E. acervulina</I> and <I>E. tenella</I> were the most prevalent, followed by <I>E. brunetti</I> and <I>E. praecox </I>(87.5, 62.5, 59.3, and 37.5% of the farms, respectively). Each of <I>E. maxima, E. mitis</I>, and <I>E. necatrix</I> was identified in 31.3% of the farms. Individual positive fecal samples contained multiple <I>Eimeria</I> species (mean=3.4). Since <I>E. maxima</I> is known to generate antigenic variants, cross-immunity was investigated for four isolates of <I>E. maxima</I> from the poultry farms in different regions of Korea. The extent of cross-protection varied from 54.3 to 100% against the heterologous isolates. The results obtained from this large-scale survey will be a useful reference for controlling coccidiosis in the poultry industry.</P>
KIM, Bongtae,LEE, Kichan,HAN, Kiwon,KIM, Duyeol,HA, Yooncheol,KIM, Chung Hyun,OH, Yeonsu,KANG, Ikjae,LEE, Jeehoon,CHAE, Chanhee Japanese Society of Veterinary Science 2010 The Journal of veterinary medical science Vol.72 No.9
<P>The aim of this study was to develop <I>in situ</I> hybridization for detection of <I>Mycoplasma hyorhinis</I> in formalin-fixed, paraffin-wax-embedded tissues from pigs with polyserositis. <I>M. hyorhinis</I> was isolated from the spleen (2 pigs) and pericardium (1 pig). <I>M. hyorhinis</I> DNA was detected 16 out of 20 pigs with polyserositis. <I>In situ</I> hybridization produced a distinct positive signal for the <I>M. hyorhinis</I> p37 gene in inflammatory cells in the polyserositis. <I>In situ</I> hybridization developed in the present study present diagnostic tools capable of detection of <I>M. hyorhinis</I> in formalin-fixed, paraffin-wax-embedded tissues from the naturally infected pigs.</P>
YU, Do-Hyeon,NOH, Dong-Ho,SONG, Ru-Hui,PARK, Jinho Japanese Society of Veterinary Science 2010 The Journal of veterinary medical science Vol.72 No.10
<P>We investigated the anti-inflammatory effects of ethyl pyruvate (EP) on LPS-stimulated canine PBMCs <I>in vitro</I>. We found that EP treatment inhibited the mRNA expressions of proinflammatory cytokines (TNF-α and IL-6), but induced mRNA expression of anti-inflammatory cytokines (IL-10). ELISA measurements revealed that EP also effectively downregulates the LPS-induced increase in proinflammatory cytokine release, while upregulating anti-inflammatory cytokine release. These data indicate that EP could be an effective anti-inflammatory agent in dogs.</P>
HWANG, In Koo,CHUNG, Jin Young,YOO, Dae Young,YI, Sun Shin,YOUN, Hwa Young,SEONG, Je Kyung,YOON, Yeo Sung Japanese Society of Veterinary Science 2010 The Journal of veterinary medical science Vol.72 No.3
<P>We compared the effects of acupuncture and electroacupuncture on cell proliferation and neuroblast differentiation using specific markers, Ki67 and doublecortin (DCX), in the subgranular zone of the dentate gyrus (SZDG) in 13-week old Wistar rats. Acupuncture and electroacupuncture were applied simultaneously in the acu-points, ST36 (Zusanli) and GV20 (Baihui), once a day for 3 weeks. Acupuncture and electroacupuncture at these acu-points significantly increased the number of Ki67-positive cells and DCX-immunoreactive neuroblasts compared to the control or sham acupuncture group. Electroacupuncture treatment significantly increased the number of well-developed (tertiary) dendrites in the SZDG compared to acupuncture treatment. These results suggest that both acupuncture and electroacupuncture increase neurogenesis in the normal, but that electroacupuncture has greater effects on neuroblast plasticity than acupuncture in the dentate gyrus.</P>