Identification of a sensitive urinary biomarker, selenium-binding protein 1, for early detection of acute kidney injury

Kim, Kyeong Seok,Yang, Hun Yong,Song, Hosup,Kang, Ye Rim,Kwon, JiHoon,An, JiHye,Son, Ji Yeon,Kwack, Seung Jun,Kim, Young-Mi,Bae, Ok-Nam,Ahn, Mee-Young,Lee, Jaewon,Yoon, Sungpil,Lee, Byung μ,Kim, Hyung TAYLOR & FRANCIS 2017 Journal of Toxicology and Environmental Health Vol.80 No.9

<P>Acute kidney injury (AKI) is associated with increased mortality rate in patients but clinically available biomarkers for disease detection are currently not available. Recently, a new biomarker, selenium-binding protein 1 (SBP1), was identified for detection of nephrotoxicity using proteomic analysis. The aim of this study was to assess the sensitivity of urinary SBP1 levels as an early detection of AKI using animal models such as cisplatin or ischemia/reperfusion (I/R). Sprague-Dawley rats were injected with cisplatin (6 mg/kg, once i.p.) and sacrificed at 1, 3, or 5 days after treatment. Ischemia was achieved by bilaterally occluding both kidneys with a microvascular clamp for 45 min and verified visually by a change in tissue color. After post-reperfusion, urine samples were collected at 9, 24, and 48 hr intervals. Urinary excretion of protein-based biomarkers was measured by Western blot analysis. In cisplatin-treated rats, mild histopathologic alterations were noted at day 1 which became severe at day 3. Blood urea nitrogen (BUN) and serum creatinine (SCr) levels were significantly increased at day 3. Levels of urinary excretion of SBP1, neutrophil gelatinase-associated lipocalin (NGAL), and a tissue inhibitor of metalloproteinase-1 (TIMP-1) were markedly elevated at day 3 and 5 following drug treatment. In the vehicle-treated I/R group, serum levels of BUN and SCr and AST activity were significantly increased compared to sham. Urinary excretion of SBP1 and NGAL rose markedly following I/R. The urinary levels of SBP1, NGAL, TIMP-1, and KIM-1 proteins excreted by AKI patients and normal subjects were compared. Among these proteins, a marked rise in SBP1 was observed in urine of patients with AKI compared to normal subjects. Based upon receiver-operator curves (ROC), SBP1 displayed a higher area under the curve (AUC) scores than levels of SCr, BUN, total protein, and glucose. In particular, SBP1 protein was readily detected in small amounts of urine without purification. Data thus indicate that urinary excretion of SBP1 may be useful as a reliable biomarker for early diagnosis of AKI in patients.</P>

CD ROM을 이용한 교육프로그램이 단기입원수술 환자의 수술 전 불안과 수술 후 자가간호 수행을 위한 지식에 미치는 효과

황연자,박연환,박인선,김남이,김정미,김진영 성인간호학회 2004 성인간호학회지 Vol.16 No.1

Purpose: The purpose of this study was to investigate the effect of nursing education using a CD ROM on the anxiety and knowledge of the patients having minor surgery. Method: Forty patients hospitalized in K hospital in Seoul from April to August 2002 participated in this study. In the experimental group, twenty patients received nursing education on the operational procedures and post-operational care. The control group, received conventional nursing care only. Anxiety experienced by patients was measured by Spielberger's State-Trait Anxiety Inventory, and knowledge was measured by an instrument developed by Rahe et al. The data were analyzed by SPSS statistical program. Result: There were no significant differences in anxiety level between two groups. However, subjects in the experimental group were found to have significantly higher postoperative knowledge levels than those in the control group, and were very satisfied with the CD ROM program. Conclusion: The nursing education program using CD ROM before minor surgery proved to be an effective nursing intervention to increase knowledge of patients and contribute to their self care after discharge. To decrease anxiety of the surgery, the nursing education program should be combined with supprortive emotional nursing intervention, such as touch, and massage.

골내부 고분화성 골육종 - 1증례 보고-

오미혜,박소영,서연림,강신광,김병직,이중달 대한병리학회 1992 Journal of Pathology and Translational Medicine Vol.26 No.6

중합효소연쇄반응으로 조사한 관절염 환자의 관절액에서 Tropheryma whipplei DNA 검출

김성연,정문현,최새로운,김은실,이진수,박원,김미경,강재승 대한감염학회 2005 감염과 화학요법 Vol.37 No.2

목적 : Whipple병은 Tropheryma whipplei에 의한 전신적인 감염 질환으로 소장과 관절 침범이 특징이며, 흔히 병의 초기에는 수년 동안 다른 증상 없이 관절염 소견만 보이므로, 일부 환자는 염증성 관절염으로 진단받을 가능성이 있다. 또한 류마티스 관절염을 포함하여 여러 염증성 관절염에서는 아직 병인이 밝혀지지 않았지만 감염이 관련될 가능성은 꾸준히 언급이 되었다. 저자들은 국내에서도 T. whipplei가 존재할 것이라는 가정 하에 한국인에서 중합효소연쇄반응으로 T. whipplei DNA를 조사하였다. 재료 및 방법 : 대상 환자군은 1997년부터 2002년 사이에 인하대병원 류마티스 내과에 관절 종창으로 내원한 환자들로, 류마티스 관절염 환자 33명, 골관절염 환자 18명, 기타 질환에서 관절 이상인 환자들로 5명이 포함되었다. 건강한 성인 8명의 타액과 22명의 위내시경을 받은 환자의 위액을 채취하여 검사하였다. 중합효소연쇄반응은 T. whipplei heat shock protein의 일부 유전자를 증폭할 수 있는 2개의 시동체를 사용하였다. 표준 균주를 얻기가 어려워 양성 대조군 없이 중합효소연쇄반응을 시행하였다. 결과 : 관절염 환자의 관절액, 정상인의 타액, 위내시경을 받은 사람의 위액에서 모두 중합효소 연쇄반응으로 핵산을 증명할 수 없었다. 결론 : 양성대조군이 없어 검사 잘못일 가능성도 배제할 수 없으나, Whipple병이 염증성 관절염의 흔한 원인이 아니거나 관절의 직접 침범보다는 위장관 감염 후 생기는 면역 반응에 의한 반응성 관절염일 가능성이 있다. 관절액 이외의 검체에서 조사한다면 다른 결과를 얻을 수도 있으리라 생각하며 앞으로 이들에 대한 조사가 필요하다. Background : Whipple's disease is a chronic systemic infection caused by Tropheryma whipplei which chiefly involves gastrointestinal tract and joints. Because arthritis is often the first sign of Whipple's disease, Whipple's disease may be misdiagnosed in the early stage of illness as non-infectious inflammatory arthritis. Rheumatoid arthritis may also be caused by infectious agents, including T. whipplei. To elucidate the relationship between T. whipplei infection and various arthritis, we examined T. whipplei DNA in Korean patients by polymerase chain reaction (PCR). Materials and Methods : With two T. whipplei-specific primers dervied from the partial heat shock protein 65 gene sequence, PCR was performed on 56 joint fluid samples : 33 samples from RA patients, 18 samples from OA patients, and 5 samples from patients with other unflammatory arthritis. In addition, the same method was applied to find out the DNA of T. whipplei in 8 normal adults' saliva and 22 patients' gastric juice. Due to the difficulty in obtaining the reference strain of T. whipplei, the PCR was performed without the positive control. Results : The PCR method did not reveal T. whipplei in joint fluid from 56 patients with arthropathies, 8 samples from saliva, and 22 samples from gastric juice. Conclusion : Though technical errors can not be excluded as a cause of absence of a positive case, our results suggest that T. whipplei infection may not be a common cause of various arthritis in Korea; T. whipplei may be associated with inflammatory arthritis indirectly by immune-mediated mechanism rather than by direct joint invasion. The examination of specimens other than joint fluid could be helpful and the study of larger number of patient is highly desirable.

정지형 자전거 운동 훈련이 건강한 여성의 체구성,심폐기능 및 유연성에 미치는 효과

김동옥,최정숙,안혜영,민혜숙,이경숙,박연환,송미령,최명애,최정안,김매자 서울대학교 간호대학 간호과학연구소 1999 간호학 논문집 Vol.13 No.1

The purpose of this study was to determine the changes in body composition, cardiopulmonary function, and flexibility following 8 weeks' bicycle ergometer exercise training. The subjects of this research consisted of healthy adult women between 30 and 40 years of age. Initial intensity of bicycle ergometer exercise was based on the target heart rate equivalent to 55% of maximal oxygen uptake, and intensity of the exercise increased by 5% every 2 weeks. Body composition, cardiopulmonary function at rest and during maximal exercise, as well as flexibility were determined before and after 8 weeks of bicycle ergometer exercise training. Masimal exercise was performed on the treadmill according to Bruce Protocol. The results obtained were as follows; 1. There were o changes in body weight, percent body fat, fat body weight, and lean body weight as well. The ventilation volume for 1 minute, respiratory quotient and expired CO₂ volume have not changed significantly after the training, either. 2. As a result of training, resting systolic and diastolic blood pressure decreased significantly(p<.05) 3. Maximal heart rate, maximal oxygen consumption and maximal running time increased significantly after the training(p<.05) 4. Pelvic flexibility increased slightly after the training, however, it did not reach the statistical significance. Trunk flexion forward increased significantly after the training(p<.05). From these results, it may be concluded that 8 weeks bicycle ergometr training improve the cardiopulmonary function and flexibility in healthy adult women between 30-40 years of age.

초등교사의 환경관련 수업에 대한 교사전문성

손연아,김경란,민병미,최돈형 한국초등과학교육학회 2005 초등과학교육 Vol.24 No.2

The purpose of this study was to analyze the professionalism of elementary school teachers and to draw out factors which elementary teachers should try to improve their professionalism for environmental education. For the study, both Quantitative analysis and qualitative methodologies were applied: First, randomly selected 483 elementary teachers from Seoul and Gyeonggi self responded to 5 evaluation areas by Hungerford et al. (1994) to evaluate their environmental education professionalism through the use of five point Likert scale. Second, 2 elementary teachers' environmental classes were observed for the purpose of analyzing strengths and weaknesses in classroom instruction, and then the results of self-evaluation on environmental education professionalism and teacher professionalism as shown in the classes were compared. It was found that 483 elementary teachers including 2 teachers for analyzing classroom instruction who performed a self-evaluation gave themselves mid-scores on the Hungerford et al.'s 5 evaluation areas. However, the result of self-evaluation did not correspond with the result of actual class observation and teacher interview. That is, the actual environmental classes conducted by 2 teachers did not seemed to reflect the evaluation areas which were the foundation of the study: the conceptual awareness, the investigation & evaluation, environmental action skills, and educational application. The results of this study can be used as the basis reference to develop teaching-learning program and in-service programs for professional development of teachers for successful application of environmental education in elementary school classes.

카드뮴폭로후 혈액과 요중 카드뮴의 변화양상

박정덕,김미정,최병선,홍연표,장임원 大韓産業醫學會 1994 대한직업환경의학회지 Vol.6 No.2

The changes of cadmium(Cd) in plasma, whole blood, erythrocyte and urine in Sprangue-Dawley male rats exposed to intravenous singleinjection of 0.8 ㎎ CdCl₂/kg of body weight were investigated. Blood was taken at 0.5 to 672 hours, and 24 hour-urine was collected by using metabolic cage for the same period. The plasma level of Cd was reached to peak at 0.5 hour after injection and reduced rapidly in 1 hour. The Cd level in blood was the highest in plasma and the lowest in erythrocyte at 0.5 hour after injection. However, in one hour postinjection, the levels of Cd were higher in order of erythrocyte, whole blood and plasma, up to 4 weeks. The changes of urinary volume and creatinine were not significant between Cd-treated and saline-treated groups. However, urinary protein was slightly increased with time in Cd-treated group. Urinary Cd level was higher in Cd treated group than control. These results suggest that the measurement of Cd in erythrocyte and urine is valuable for the biological index to estimate recent Cd exposure.

神門穴 鍼刺戟이 母性分離 스트레스로 야기된 攝食 障碍와 시상하부 neuropeptide Y 발현에 미치는 영향

박히준,류연희,홍미숙,김승태,임사비나 WHO COLLABORATING CENTRE FOR TRADITIONAL MEDICINE 2003 東西醫學硏究所 論文集 Vol.2003 No.-

Objective : The purpose of this study is to find out the effect of acupuncture at HT7(Shinmun) on the feeding behavior and hypothalamic neuropeptide Y(NPY) expression in the maternally separated rat pups. Methods : To show the effect of acupuncture, we performed maternal separation(MS) for 7 days beginning on postnatal day 14, and observed body weight, food intake, and NPY immunoreactivity in the paraventricular nucleus(PVN) of hypothalamus after acupuncturing at HT7, the end of the transverse crease of the ulnar wrist of the forepaw. Results : MS induced a significant decreases in body weight and food intake, while acupuncture treatment at acupoint HT7 showed much more improvement in those evaluations. NPY-immunoreactivity in area PVN were decreased in the MS group, but significantly increased in the HT7 group. Conclusions : These findings suggest that acupuncture has an effect on the feeding disorders caused by MS, possibly by modulating NPY expression in the PVN.

Infectious Keratitis in Patients with Ocular Sjögren’s Syndrome

So Yeon Kim(So Yeon Kim),Seong Hwan Kim(Seong Hwan Kim),Chang Ho Yoon(Chang Ho Yoon),Mee Kum Kim(Mee Kum Kim),Joo Youn Oh(Joo Youn Oh) 대한안과학회 2022 Korean Journal of Ophthalmology Vol.36 No.5

Purpose: To investigate the incidence, characteristics, risk factors, and treatment outcomes for infectious keratitis in patients with ocular Sjögren’s syndrome (SS). Methods: We performed a retrospective chart review of patients who had been followed up for ocular SS in Seoul National University Hospital from 2010 to 2020 and identified cases where infectious keratitis developed. The incidence, demographical and clinical characteristics, risk factors, microbiological profiles, and treatment outcome were investigated, some of which were compared with infectious keratitis cases in the non-SS group. Results: Out of 929 patients with ocular SS, infectious keratitis occurred in 18 eyes (1.94%). All 18 patients were female in the ocular SS group, while 48 out of 100 infectious keratitis patients (48%) were female in the non-SS group (p < 0.01). The mean age at diagnosis of infectious keratitis was 66.1 years in the ocular SS group, which was not different from the non-SS group (57.2 years, p = 0.12). Of risk factors analyzed, the use of therapeutic contact lens was more frequently used in the ocular SS patients, compared to the non-SS patients (67% vs. 11%, p < 0.01). Culture-positivity rate was 50% in the ocular SS group. All culture-proven cases were bacterial infection, one of which was bacterial-fungal coinfection. Infection resolved in all eyes after the mean 29 days of medical treatment, except one that additionally required penetrating keratoplasty with vitrectomy. The visual acuity improved in 15 eyes (83%) after resolution. Infectious keratitis recurred in three patients (17%) during the mean 55.7 months of follow-up. Conclusions: The incidence of infectious keratitis was 1.94% in patients with ocular SS. Most were bacterial infections and resolved by medical treatment. Therapeutic and visual outcomes were favorable, but recurrence occurred in 17%.

Loss-of-function screens of druggable targetome against cancer stem–like cells

Song, Mee,Lee, Hani,Nam, Myung-Hee,Jeong, Euna,Kim, Somin,Hong, Yourae,Kim, Nayoung,Yim, Hwa Young,Yoo, Young-Ji,Kim, Jung Seok,Kim, Jin-Seok,Cho, Yong-Yeon,Mills, Gordon B.,Kim, Woo-Young,Yoon, Sukjo Federation of American Societies for Experimental 2017 The FASEB Journal Vol.31 No.2

<P>Cancer stem–like cells (CSLCs) contribute to the initiation and recurrence of tumors and to their resistance to conventional therapies. In this study, small interfering RNA (siRNA)-based screening of ∼4800 druggable genes in 3-dimensional CSLC cultures in comparison to 2-dimensional bulk cultures of U87 glioma cells revealed 3 groups of genes essential for the following: survival of the CSLC population only, bulk-cultured population only, or both populations. While diverse biologic processes were associated with siRNAs reducing the bulk-cultured population, CSLC-eliminating siRNAs were enriched in a few functional categories, such as lipid metabolism, protein metabolism, and gene expression. Interestingly, siRNAs that selectively reduced CSLC only were found to target genes for cholesterol and unsaturated fatty acid synthesis. The lipidomic profile of CSLCs revealed increased levels of monounsaturated lipids. Pharmacologic blockage of these target pathways reduced CSLCs, and this effect was eliminated by addition of downstream metabolite products. The present CSLC-sensitive target categories provide a useful resource that can be exploited for the selective elimination of CSLCs.—Song, M., Lee, H., Nam, M.-H., Jeong, E., Kim, S., Hong, Y., Kim, N., Yim, H. Y., Yoo, Y.-J., Kim, J. S., Kim, J.-S., Cho, Y.-Y., Mills, G. B., Kim, W.-Y., Yoon, S. Loss-of-function screens of druggable targetome against cancer stem–like cells.</P>