Identification of Genes Modulated by High Extracellular Calcium in Coculture of Mouse Osteoblasts and Bone Marrow Cells by Oligo Chip Assay

Kim, Hyung-Keun,Song, Mina,Jun, ji-Hae,Woo, Kyung-Mi,Kim, Gwan-Shik,Baek, Jeong-Hwa The Korean Academy of Oral Biology 2006 International Journal of Oral Biology Vol.31 No.2

Calcium concentration in the bone resorption lacunae is high and is in the mM concentration range. Both osteoblast and osteoclast have calcium sensing receptor in the cell surface, suggesting the regulatory role of high extracellular calcium in bone merabolism. In vitro, high extracellular calcium stimulated osteoclastogenesis in coculture of mouse osteoblasts and bone marrow cells. Therefore we examined the genes that were commonly regulated by both high extracellular calcium and 1,25(OH)_(2)vitaminD_(3)(VD3) by using mouse oligo 11 K gene chip. In the presence of 10 mM[Ca^(2+)]e or 10 nM VD3, mouse calvarial osteoblasts and bone marrow cells were co-cultured for 4 days when tartrate resistant acid phosphatase-positive multinucleated cells start to appear. Of 11,000 genes examined, the genes commonly regulated both by high extracellular calcium and by VD3 were as follows; 1) the expressions of genes which were osteoclast differentiation markers or were associated with osteoclastogenesis were up-regulated both by high extracellular calcium and by VD3; trap, mmp9, car2, ctsk, ckb, atp6b2, tm7sf4, rab7, 2) several chemokine and chemokine receptor genes such as sdf1, scya2, scyb5, scya6, scya8, scya9, and ccr1 were up-regulated both by high ectracellular calcium and by VD3, 3) the genes such as mmp1b, mmp3 and c3 which possibly stimulate bone resorption by osteoclast, were commonly up-regulated, 4) the gene such as c1q and msr2 which were related with macrophage function, were commonly down-regulated, 5) the genes which possibly stimulate osteoblast differentiation and/or mineralization of extracellular matrix, were commonly down-regulated;slc8a1, admr, plod2, lox, fosb, 6) the genes which possibly suppress osteoblast differentiation and/or mineralization of extracellular matrix, were commonly up-regulated;s100a4, npr3, mme, 7) the genes such as calponin 1 and tgfbi which possibly suppress osteoblast differentiation and/or mineralization of extracellular matrix, were up-regulated by high extracelluar calcium but were down-regulated by VD3. These results suggest that in coculture condition, both high extracellular calcium and VD3 commonly induce osteoclastogenesis but suppress osteoblast differentiation/mineralization by regulating the expression of related genes.

Protective Effects of N-acetylcystenine and Selenium against Doxorubicin Toxicity in Rats

Yeo-sung Yoon,Sun-don Kim,Min-hye Lee,Heung-shik S.Lee,In-se Lee,Je-kyung Sung,Eun-sung Park 대한수의학회 2003 Journal of Veterinary Science Vol.4 No.2

Effects of N-acetylcysteine and Selenium against Doxorubicin Toxicity in RatsEun-sung Park, Sun-don Kim1, Min-hye Lee, Heung-shik S. Lee, In-se Lee, Je-kyung Sung and Yeo-sung Yoon*

한의진단명과 진단요건의 표준화 연구 III : 3차년도 연구결과 보고

최선미,양기상,최승훈,박경모,박종현,심범상,김성우,노석선,이인선,정진홍,이진용,김달래,임형호,김윤범,박성식,송태원,김종우,이승기,최윤정,신순식 한국한의학연구원 1997 한국한의학연구원논문집 Vol.3 No.1

The diagnostic requirements were suggested and explained regarding the systems of differentiation of symptoms and signs in the third year study of standardization and unification of the terms and conditions used for diagnosis in oriental medicine. The systems were as follows : - analyzing and differentiating of epidemic febrile disease - analyzing and differentiating in accordance with the Sasang constitution medicine based on four-type recognition - differentiation of disease according to pathological changes of Chong and Ren channels - standards for diagnosis of women's disease - standards for diagnosis of children's disease - standards for diagnosis of motor and sensor disturbance (-muscle. born, joint, etc.) - standards for diagnosis of neuropsychiatric disease - standards for diagnosis of five sense organ disease - standards for diagnosis of external disease The indivisual diagnosis pattern was arranged by the diagnostic requirements in the following order : another name, notion of diagnosis pattern, index of differentiation of symptoms and signs, the main point of diagnosis, analysis of diagnosis pattern, discrimination of diagnosis pattern, prognosis, a way of curing a disease, prescription, herbs in common use, disease appearing the diagnosis pattern, documents. The standards for diagnosis of each disease was arranged by the diagnostic requirements in the following order : another name, notion of disease, the main point of diagnosis, analyzing and differentiating of disease, analysis of disease, discrimination of disease, prognosis, a way of curing and prescription of disease, disease in western medicine appearing the disease in oriental medicine, documents.

社會敎化로서의 鄕約에 關한 一考察 : 그 槪念·成立·受容過程을 中心으로

金璟植 圓光大學校 敎育問題硏究所 1984 敎育硏究 Vol.- No.3

음향방출 신호처리에 의한 플라즈마용사코팅 피막부식재의 파손해석

김귀식,박경석,홍용의,현창해 제주대학교 산업기술연구소 2001 尖端技術硏究所論文集 Vol.12 No.1

This paper was to investigate of a adhesiveness of the plasma sprayed coating materials did a salt spray by acoustic emission method in tensile loading. The powders used for the coating were nickel aluminum composite powder Ni-4.5wt.%Al and titanium dioxide powder TiO2. These powders were coated on a carbon steel S45C by plasma spray method. The salt solution was a 5% NaCl and the salt spray times were 2, 5 and 10 hours respectively. The salt solution penetrated into the surface of the substrate through pore of the coating layer built in the process of plasma spay. Corrosion productions formed on the surface of substrate. The adhesiveness between the substrate and the coating layer is weaken by corrosion and the exfoliation initiated chiefly at the corrosion surface of the substrate. The AE events and energy of the corroded coating specimens decreased as the salt spray times increased. The AE amplitudes increased at strain range of 6%-11%.

급성백혈병환자에서 분리된 Staphylococcus epidermidis의 분자 역학적 특성 및 위험요인

김경미,이동건,김상일,최정현,한성숙,신완식 대한병원감염관리학회 2003 의료관련감염관리 Vol.8 No.2

企業引受와 長期經營成果와의 關係 : 거평그룹을 中心으로 Focused on Keopyung Group

金熙錫,曺敬植 大邱大學校 社會科學硏究所 1999 社會科學硏究 Vol.6 No.2

본 연구에서는 거평그룹의 타기업 인수가 장기적인 관점에서 그 기업의 경영성과에 어떠한 영향을 미치고 있는가를 분석하기 위하여 기업의 경영성과를 측정하는 가장 대표적인 지표로 인정받고 있는 총자본순이익률(ROA), 자기자본순이익률(ROE), 매출액순이익률(ROS)을 기준으로 인수전·후의 기업경영성과를 t-검정을 통하여 측정하였다. 분석결과 거평인수기업의 ROA, ROE, ROS와 산업평균지표간에는 유의적인 차이가 없는 것으로 밝혀졌는데, 이것은 거평그룹의 인수기업 경영성과가 인수로 인해 산업평균에 비해서 더 나아지지는 않았음을 나타낸다. 반면에 기업의 위험은 인수이전에 비해 더욱 증가하였는데, 이러한 현상은 거평그룹의 기업인수 역시 과거 대다수 우리나라 기업의 M&A에서와 같이 기업경영성과의 향상 또는 株主富의 극대화보다는 量的 外形불리기에 급급하였음을 보여 주는 것이라 할 수 있다.

多衆利用施設의 消防安全管理 强化方案에 관한 硏究

김유식,정경문 진주여자전문대학 2000 論文集 Vol.23 No.-

After the IMF bailout in 1998, redirection of public enterprises and the results of mass unemployment caused various changes in the o v d society. Multi-use facilities such as KARAOKES, bars, Internet rooms, and others were established due to easy availability of retirement funds allocated to the laid-off employees. Because of this trend, the number of these types of business ventures has rapidly increased. Many human lives and property losses are in jeopardy in case of a fire due to an establishment s basement arrangement, especially within a multi-use facility. In addition, the diversity and complexity of the internal setup of the establishment place great risk to its clientele. Among the disasters, most fires are the result of inadequate fire protection installation and careless management of related personnel. Management s lack of concern over fire safety installation is the primary cause of fires. However, fire exits and evacuation procedures and fire extinguishers are critical to minimize the loss of human lives and property. The recent fires in HAWSUNG SEALAND and INCHUN LIFE HOF and others could understandably occur in underdeveloped countries where fire-fighting measures are limited. But these fires easily erupted in our country. These days, human mishandling rather than natural forces causes the origin of most fires and the number of these incidents is increasing everyday.

ESPI를 이용한 복합재료 구조물의 결함 검출

김경석,정성균,강진식,장호섭 한국비파괴검사학회 2001 한국비파괴검사학회지 Vol.21 No.3

본 논문에서는 ESPI 시스템을 이용하여 복합재료 구조물의 인위, 자연 결함을 검출하였다. 복합재료 구조물에서의 ESPI의 적용성을 알아보기 위해 복합재료 적층판 시험편, 하니컴 구조물 시험편, 접착조인트 시험편을 사용하였다. 결함을 검출하기 위해 시편의 표면변형을 쉽게 발생시킬 수 있는 열하중법을 선택하였다. 실험경과는 ESPI를 이용하여 복합재료 구조물의 결함을 쉽게 검출할 수 있고, 다른 여러 복합재료 구조물의 결함의 검출에도 적용할 수 있음을 확인하였다. In this paper, artificial and real defects(delamination and debond) in composite structures were detected by using ESPI system. There types of specimens, that is, composite laminates, honeycomb structures, and adhesive joints, were used to study the applicability of ESPI to composite structures. To detect defects in specimens, we selected thermal loading method that can easily induce the surface deformation of specimen. Experimental results show that defects in composite structures could be easily detected by ESPI. Moreover, it shows that ESPI could be usefully applied to the detection of defects of defects in various composite structures.

자기공명영상으로 진단된 바이러스성 능형뇌염

김재영,서경식 건국대학교 의과학연구소 1994 건국의과학학술지 Vol.4 No.-

Viral neuritis due to herpes simplex type II has been well documented. The virus lies dormant within the gasserian ganglion of the trigeminal verve. Retrograde spread into the brainstem is rare. This has been referred to as rhombencephalitis. The reported cases of rhombencephalitis detected by MR are very rare. So we report a case of viral rhombencephalitis which was diagnosed by MR. A 35 year-old female patient was admitted due to dizziness, nausea, tinnitus(Lt.), hearing impairment(Lt.), and hypoesthesia on the left face, which were similar to the symptoms of acoustic neuroma. MR showed the enhanced high signal intensity in left middle cerebellar peduncle. One month after treatment, the symptoms resolved. Follow-up MR showed the decreased high signal intensity. We report this case with review of the literature.