Quantitative Assay for the Binding of Jun-Fos Dimer and Activator Protein-1 Site

Lee, Sang-Kyou,Park, Se-Yeon,Jun, Gyo,Hahm, Eun-Ryeong,Lee, Dug-Keun,Yang, Chul-Hak Korean Society for Biochemistry and Molecular Biol 1999 Journal of biochemistry and molecular biology Vol.32 No.6

The Jun and Fos families of eukaryotic transcription factors form heterodimers capable of binding to their cognate DNA enhancer elements. We are interested in searching for inhibitors or antagonists of the binding of the Jun-Fos heterodimer to the activator protein-1 (AP-1) site. The basic-region leucine zipper (bZIP) domain of c-Fos was expressed as a fusion protein with glutathione S-transferase, and allowed to form a heterodimer with the bZIP domain of c-Jun. The heterodimer was bound to glutathione-agarose, to which were added radiolabeled AP-1 nucleotides. After thorough washing, the gel-bound radioactivity was counted. The assay is faster than the coventional electrophoretic mobility shift assay because the gel electrophoresis step and the autoradiography step are eliminated. Moreover, the assay is very sensitive, allowing the detection of picomolar quantities of nucleotides, and is not affected by up to 50% dimethylsulfoxide, a solvent for hydrophobic inhibitors. Curcumin and dihydroguaiaretic acid, recently known inhibitors of Jun-Fos-DNA complex formation, were applied to this Jun-GST-fused Fos system and revealed to decrease the dimer-DNA binding.

Quantitative Assay for the Binding of Jun-Fos Dimer and Activator Protein-1 Site

Yang, Chul Hak,Jun, Gyo,Lee, Dug Keun,Lee, Sang Kyou,Park, Se Yeon,Hahm, Eun Ryeong 생화학분자생물학회 1998 BMB Reports Vol.32 No.6

The Jun and Fos families of eukaryotic transcription factors form heterodimers capable of binding to their cognate DNA enhancer elements. We are interested in searching for inhibitors or antagonists of the binding of the Jun-Fos heterodimer to the activator protein-1 (AP-1) site. The basic-region leucine zipper (bZIP) domain of c-Fos was expressed as a fusion protein with glutathione S-transferase, and allowed to form a heterodimer with the bZIP domain of c-Jun. The heterodimer was bound to glutathione-agarose, to which were added radiolabeled AP-1 nucleotides. After thorough washing, the gel-bound radioactivity was counted. The assay is faster than the conventional electrophoretic mobility shift assay because the gel electrophoresis step and the autoradiography step are eliminated. Moreover, the assay is very sensitive, allowing the detection of picomolar quantities of nucleotides, and is not affected by up to 50% dimethylsulfoxide, a solvent for hydrophobic inhibitors. Curcumin and dihydroguaiaretic acid, recently known inhibitors of Jun-Fos-DNA complex formation, were applied to this Jun-GST-fused Fos system and revealed to decrease the dimer-DNA binding.

Quantitative Assay for the Binding of Jun-Fos Dimer and Activator Protein-1 Site

Yang,Chul-Hak,Jun,Gyo,Hahm,Eun-Ryeong,Lee,Sangkyou,Park,Seyeon,Lee,Dug-Keun The Korea Science and Technology Center 1999 BMB Reports Vol.32 No.6

The Jun and Fos families of eukaryotic transcription factors form heterodimers capable of binding to their cognate DNA enhancer elements. We are interested in searching for inhibitors or antagonists of the binding of the Jun-Fos heterodimer to the activator protein-1 (AP-1) site. The basic-region leucine zipper (bZIP) domain of c-Fos was expressed as a fusion protein with glutathione S-transferase, and allowed to form a heterodimer with the bZIP domain of c-Jun. The heterodimer was bound to glutathione-agarose, to which were added radiolabeled AP-1 uncleotides. After thorough washing, the gel-bound radioactivity was counted. The assay is faster than the coventional electrophoretic mobility shift assay because the gel electrophoresis step and the autoradiography step are eliminated. Moreover, the assay is very sensitive, allowing the detection of picomolar quantities of nucleotides, and is not affected by up to 50% dimethylsulfoxide, a solvent for hydrophobic inhibitors. Curcumin and dihydroguaiaretic acid, recently known inhibitors of Jun-Fos-DNA complex formation, were applied to this Jun-GST-fused Fos system and revealed to decrease the dimer-DNA binding.

An Investigation on the Life of Master Won Guk Lee, the Creator of Chung-Do-Kwan

( Seon Young Jung ),( Jun Ho Lee ),( Seung Woo Shin ),( Chun Hwan Cho ),( Seung Hyun Jang ),( Won Shin ) 경남대학교 기초과학연구소 2014 기초과학지 Vol.31 No.-

The purpose of this study is to review (or, investigate) the life of Won Guk Lee. He is the creator of Chung-Do-Kwan, which is one of the five original Taekwondo kwans. Data was gathered from advanced research, newspapers, documentaries, the Internet, magazines, and journals etc. Thus, we were able to create a sequence of events based on the research data. First, we can say that in Won Guk Lee``s career, he created Chung-Do-Kwan and brought Dangsoodo to Korea. These events have had a significant development on Taekwondo. Second, Taekwondo curriculum, which was published by Won Guk Lee, established the foundation of Taekwondo poomsae, as well as giving us the pure spirit of Korean martial arts.

소음인(少陰人) 태음병(太陰病) 심하비증(心下?證)의 병증약리(病證藥理) 고찰

서영광,이지원,이준희,최원철,이의주,고병희,Seo, Young-Kwang,Lee, Ji-Won,Lee, Jun-Hee,Choi, Won-Cheol,Lee, Eui-Ju,Kho, Byung-Hee 사상체질의학회 2010 사상체질의학회지 Vol.22 No.3

1. Objective: Gastric Stuffiness (心下?證) in the Soeumin Taeeum symptomatology (少陰人 太陰病) in Sasang Constitutional Medicine shows an interesting evolutionary process. Originally found in the texts of Sanghanlun (傷寒論), the perspectives regarding the symptoms of Gastric Stuffiness were expanded by many medical scholars and Lee Jema. To comprehend the Jema's concept of Taeeum symptomatology and utilize his prescription clinically, it deserves to study on the Gastric Stuffiness in Donguisuseowon (東醫壽世保元). 2. Methods: Many texts including Sanghanlun (傷寒論) and Dongeuibogam (東醫寶鑑) were reviewed in order to track the evolutionary process concerning the symptoms of Gastric Stuffiness. Also, Lee Jema's works including the different versions of Donguisuseowon (東醫壽世保元) were reviewed to examine the development of Lee's concepts regarding the Gastric Stuffiness in the Soeumin Taeeum symptomatology. 3. Results and Conclusions: 1) Most of the previous concepts on Gastric Stuffiness were generally similar to those found in Sanghanlun, especially in that they addressed Yang deficiency of the middle triple energizeer as the central pathogenic process. 2) Lee Jema's views on the epigastric discomfort in the Soeumin Taeeum symptomatology evoloved over time and culminated in the finalized pathology and therapeutics appearing in his last work, the Shinchuk version of Donguisuseowon (東醫壽世保元). 3) 4 kinds of prescription which invented by Lee Jema to treat the Gastric Stuffiness in the Soeumin Taeeum symptomatology can be matched to the prescription of Sanghanlun respectively. However through evolutionary process of Jema's prescriptions, the indication of them expanded: not only to treat Gastric Stuffiness but to diverse symptoms of Soeumin.

고요산혈증의 위험인자 : 아산시 지역 주민을 대상으로

김용배,장원기,황보영,김화성,안규동,이병국,이용진,이종은,이준숙,이찬구,이한정,이성수 순천향대학교 2006 Journal of Soonchunhyang Medical Science Vol.12 No.1

Objective : Serum uric acid has now been identified as a marker for a number of metabolic and hemodynamic abnormalities. The aim of this study is to evaluate risk factors which influence hyperuricemia in the citizens of Asan. Method : The study design was cross-sectional and research subjects were 1086 citizens of Asan. General characteristics, cigarette smoking, alcohol consumption, and past history were obtained by interview using a structural questionnaire. Also, we measured height, weight and blood pressure, and serum uric acid of the subjects. Results : The mean serum uric acid level was 5.27 ㎎/㎗ in men and 4.01 ㎎/㎗, in women, In the multiple logistic regression analysis, statically significant risk factors of hyperuhcemia were gender, body mass index, blood lead concentration, and serum creatinine. Conclusion : Our results suggest that gender, body mass index, blood lead concentration, and serum creatinine might be the most important risk factors of hyperurcemia. Education program as well as routine check-up for serum uric acid was highly recommended for the effective prevention of hyperuricemia.

Insecticidal toxicities of carvacrol and thymol derived from Thymus vulgaris Lin. against Pochazia shantungensis Chou & Lu.

Jun?Hwan Park,Na-Hyun Lee,Sang-Guei Lee,Hoi-Seon Lee 한국농약과학회 2017 한국농약과학회 학술발표대회 논문집 Vol.2017 No.4

Effect of Impact Velocity on Interface Characteristics of HT‑9 Steel Joints Fabricated by Magnetic Pulse Welding

Jun‑Woo Song,Jin‑Ju Park,Gyoung‑Ja Lee,Min‑Ku Lee,Kyu‑Hyun Park,Soon‑Jik Hong,Jung Gu Lee 대한금속·재료학회 2020 METALS AND MATERIALS International Vol.26 No.3

The weldability window in magnetic pulse welding (MPW) is proposed for the end-closure joining of HT-9 steel tubularworkpieces. Based on both theoretical and experimental investigations, the impact velocity of the flyer tube at the momentof collision had a dominant effect on the bonding characteristics of the resultant joints and the optimal impact velocity wasestimated to be in the range of 265–290 m/s in this alloy system. Under the optimal process conditions, no leak was detectedduring helium leak testing. Moreover, the integrity of the joint interface was preserved until the tube section ruptured duringinternal pressure burst testing. For HT-9 steel, the ductile δ-ferrite phase produced by the localized interfacial melting duringMPW acted as an effective obstacle to crack propagation, thereby enhancing the fracture resistance of the joint interface. Allof the results demonstrated the high integrity and durability of the HT-9 steel tube assembly fabricated by the MPW process.

휴대폰 카메라용 자동초점 구동기의 충격해석

이성민,김봉석,송준호,이수훈,이혜진,이문구,송준엽,이창우 한국공작기계학회 2008 한국공작기계학회 춘계학술대회논문집 Vol.2008 No.-

Recently, the robustness against daily impact is important according to portablization and downsizing of mobile electronics. Especially, almost parts of cellular phone should undergo drop test when they fall 1.5 m above ground. This test simulates the case when cellular phone slips through user's fingers while he is talking on the phone. This paper studies a drop test of auto focusing (AF) actuator for camera module in cellular phone. This component is composed of voice coil motor (VCM) as an actuator and leaf spring as a guide and suspension. The leaf spring's deformation is essential for the test because its permanent distortion disables the focusing, and then, a high quality photograph cannot be obtained. Up to now the drop test has been carried out after fabricating real AF actuator. We propose a dropt test model which simulates the drop test based on finite element analysis. This model makes us enable investigate the stress acting on the clamping and curved parts of leaf spring. Stress over Von Mises criterion lets the spring deformed permanently, and then AF actuator malfunctioned, It helps us to design and modify AF actuator without manufacturing the real product. And also, it saves the time and cost for the development of new products.

홉스의 『시민론』과 『인간론』을 중심으로 본 인권과 전쟁의 권리

이준호 ( Jun-ho Lee ) 서양근대철학회 2018 근대철학 Vol.12 No.-

이 글은 고 이준호 박사의 유고논문이다. 저자가 세운 이 글의 목적은 분명하다. 홉스 연구자들이 『리바이어던』을 main text로 삼고 있는 것에서 벗어나 『시민론』과 『인간론』의 중요성에 주목하고 있다. 필자는 홉스의 시민론을 직접 우리말로 번역한 바 있다. 이 번역 작업을 통해 시민론에 대한 깊은 이해와 성찰을 바탕으로 홉스 정치철학을 새롭게 해석할 가능성을 모색하고 있다. 그가 주목한 새로운 해석이란, 『시민론』에서 홉스는 전쟁을 적극적으로 반대하고 있다는 사실과 신민들의 인권 보호와 복지 증진을 위해 군주가 져야할 자연적 의무가 있다는 것을 보여주는 일이다. 또 최고통치권자에게 복종해야만 하는 근거는 통치권자 자신이 소유한 힘에 대한 ‘공포’에 있지 않고, 실정법을 위반했을 때 받게 되는 처벌의 공포에 무게 중심을 두고 있다. 3절에서 필자는 국가의 두 기원, 동의와 정복에 관해 분석하고 있다. 이 절에서 필자는 실질적 사회계약론에 대해 의문을 제기하고 있다. 애초 국가는 자연발생적으로 시작되었으며, 최고 통치권자의 자의적 통치를 견제하기 위해 신민들과 계약을 맺게 된 것이라는 필자의 해석이다. 4절에서 필자는 시민론의 열 번째 자연법에 주목하고 있다. 공정성, 즉 차별하지 말라는 자연법의 명령은 국가의 평화와 안위를 위해서 통치권자가 지켜야 할 덕목이라는 것이다. 특히 분배의 공정성을 유지하는 일이 통치권자의 의무이다. 5절은 통치권력의 절대성과 복종에 대해 설명하고 있다. 정당화된 권력은 국가의 안전을 위해 신민들의 사생활까지 감시할 수 있어야 한다고 주장한다. 당연히 오늘날의 시각에서 보면 인권침해의 위험이 있지만 홉스 당시에는 문제가 되지 않는다. 그러나 홉스는 부당한 권력에 대해서는 자발적 복종의 의무를 배제하고 있다. 통치권자도 양심과 자연법에 따라 통치할 때만 권력은 정당화되며, 신민들의 자발적 복종을 얻을 수 있다. 6절에서 필자는 자연법을 위반한 통치권자에 대한 전쟁의 권리에 대해 설명하고 있다. 신민을 적으로 만든 통치권자는 그 권력의 정당성을 상실하게 되고, 신민들은 통치권자를 향해 전쟁의 권리를 회복하게 된다는 것이다. 챨스 1세의 처형이 이에 해당된다. 결론에서는 홉스의 정치철학사적 위치를 다시 규정하고 있다. 홉스는 절대왕권주의를 지지하면서도 주권재민론의 선구자로서의 자리매김을 하고 있다. This is a paper by Dr. Lee Jun-ho who died a few years ago. The purpose of this article is clear. Dr. Lee suggests that Hobbes researchers should pay attention to the importance of De Cive and De Homine, not to consider Leviathan as the main work of Hobbes. He translated Hobbes’ De Cive and De Homine into Korean. Through this translation work, he was looking for a possibility to interpret Hobbes’ political philosophy based on deep understanding and reflection on civil theory. The new interpretation he notices is that Hobbes is actively opposed to war in the De Cive and shows that there is a natural obligation of the monarch to protect the human rights and welfare of the people. The author emphasizes that the reason for submitting to the supreme ruler is not the “fear” of the powers held by the ruling power, but the fear of punishment in violation of the law. In section 3, the author analyzes the origins of consent and conquest. In this section, he questions the actual social contract theory. It is his interpretation that the country originally started spontaneously and that it had entered into a contract with the people to check the arbitrary rule of the supreme ruler. In section 5, the author explains the absoluteness and obedience of ruling power. He maintains that justified power has to monitor the privacy of the people for the sake of national security. Of course, from today’s point of view there is a risk of human rights violations, but it is not a problem at the time. However, Hobbes excludes the obligation of voluntary obedience to unjust power. Only when the ruling power is governed by conscience and natural law, power is justified and the voluntary obedience of the people can be obtained. In section 6, the author explains the right of war to the rulers who violate natural law. The rulers who have made the enemies of the people lose the legitimacy of their power, and the people will regain the right of war to the rulers. This is the execution of Charles I. In conclusion, the author redefines the historical location of Hobbes’s political philosophy. According to the author, Hobbes has always been a pioneer of sovereignty, while supporting absolutism.