CdSe ; Cu 광전도 cell 제작

신영진,정태수,신현길,김택성,정철훈,이훈,신영신 경북대학교 센서기술연구소 1992 센서技術學術大會論文集 Vol.3 No.1

SWAT 모형을 이용한 태화강유역 장기유출 분석

신현석(Shin Hyun Suk),강두기(Kang Du Kee),김균태(Kim Kyun Tae) 대한토목학회 2004 대한토목학회 학술대회 Vol.2004 No.10

구리 독성에 기인하는 비정상적인 어류행동의 신경생화학적 분석

신성우,조현덕,전태수,김정상,이성규,고성철 한국환경독성학회 2003 환경독성보건학회지 Vol.18 No.2

The goal of this study is to develop a biomarker used in monitoring abnormal behaviors of Japanese medaka (Oryzias latipes) as a model organism caused by hazardous chemicals. Japanese medaka was treated by copper of appropriate sublethal concentrations after starvation for 48 hr, The untreated individuals showed common behavioral characteristics (i.e., smooth and linear movements). Locomotive activity of the fish was monitored using an image processing and automatic data acquisition system. When treated with copper (100 ppb), the fish showed shaking patterns more frequently. As the concentration of copper increased to 1,000ppb, activity decreased, and the fish showed an erratic movement. Fish were exposed to copper at various concentrations (0, 100 and 1,000 pph) for 24 hrs, and acetylcholine esterase (AChE) activity was observed. When fish were exposed to 1,000 ppb of copper, the body AChE activities appeared to decrease but the head AChE activities showed little change. Expressions of tyrosine hydroxylase (TH) protein in the different organs from both head (brain) and body(kidney) portions affected by the copper treatment were analyzed using immunohistochemical technique compared with control. Five organs of the fish (olfactory bulb, hyothaiamus, optic lobe, pons and myelencephalon regions) showed a relatively strong TH protein expression in the control experiment. A differential expression of TH, however, was observed in the treatment (100 ppb and 1,0M ppb). The treatment (1,000 ppb) significantly suppressed TH protein production in the brain regions. In kidney, however, the same treatment caused little suppression compared with the control. Copper appeared to be less effective in suppression of TH than diazinon, a known TH suppressor. It was concluded that TH could be used as a potential biomarker to monitor the acute copper toxicity in Japanese medaka.

간외담관 폐쇄에서의 초음파소견 : 담관벽 경계소실의 중요성 Significance of Decreased Echotexture of Bile Duct Wall-Interface

신현준,손은주,이창희,최영칠,전혜정,박정희,이태의 건국대학교 의과학연구소 2002 건국의과학학술지 Vol.12 No.-

Ultrasound is very useful in evaluation of obstructive jaundice. We retrospectively evaluated the useful findings of ultrasonography in differentiating benign extrahepatic duct obstruction from malignant diseases. Among the 36 patients, 25 patients were benign extrahepatic obstruction and 11 patients were malignant obstruction. The shapes of extrahepatic dilatation were classified into fustiform, meniscus, and abrupt cut off by bowel gas. The diameter, length, and bile duct wall-interface were compared between benign and malignant groups and serum bilirubin and alkaline phosphatase were also compared between two groups retrospectively. The results were as follows. The most common form of extrahepatic dilatation was meniscus(10 of 26 patients, 38%) in benign obstruction, and fusiform(8 of 11 patients, 73%) in malignant obstruction. No significant difference was noted in diameter, length, serum bilirubin, and alkaline phosphatase between benign and malignant groups. Only one case showed decreased bile duct wall-interface in benign obstruction, but loss or decrease of bile duct wall-interface were 8 patients in malignant obstruction. The combined findings of fusiform dilatation and loss of bile duct wall-interface in ultrasonography will be helpful in differential diagnosis of malignant extrahepatic bile obstruction from benign conditions.

Fluoranthene 독성에 기인하는 비정상적 어류행동의 신경생화학적 분석

신성우,조현덕,전태수,김정상,이성규,고성철 한국환경독성학회 2003 환경독성보건학회지 Vol.18 No.2

Fluoranthene, a common polycyclicaromatic hydrocarbon (PAH), exhibits phototoxicity which may affect aquatic organisms, The eventual goal of this study is to develop a biomarker of Japanese medaka (Oryzias iatipes) used in monitoring hazardous chemicals in the ecosystem. In this study we investigated neural toxicity of fluoranthene in Japanese medaka (Oryzias latipes) along with comparative analysis of corresponding behavioral responses. The untreated individuals showed normal behavioral characteristics (i.e' smooth and linear movements). The treated fish, however, showed stopping and abrupt change of orientation (100 ppb), and severely reduced locomotive activity and enhanced surfacing activity (1,000ppb). Treatment of the medaka fish with fluoranthene caused a significant suppression of acetylcholine esterase (AChE) activities in the body portion but not in the head portion. When fish were exposed to 1,000pph of fluoranthene for 24hr, the body AChE activities decreased from 126.±31.89 (nmoles substrate hydrolyzed per min per mg protein) 10 49.51 ± 11.99. Expressions of tyrosine hydroxylase (TH) protein in the different organs from both head and body portions were comparatively analyzed using an immunohistochemical technique. Five organs of the medaka fish showing a strong TH protein expression were the olfactory bulb, hypothalamus, optic lobe, pons and myelencephalon regions. This study provides molecular and neurobehavioral bases of a biomonitoring system for toxic chemicals using fish as a model organism.

골격성 전치부 반대교합 아동의 치아성숙도에 관한 연구

신종현,권민석,김신,정태성 大韓小兒齒科學會 2010 大韓小兒齒科學會誌 Vol.37 No.3

전치부 교환기의 반대교합 아동에서는 골격적 특성이 강할수록 상하 치아의 성숙도에 현격한 차이가 있음을 쉽게 관찰할 수 있다. 파노라마 방사선 사진으로 평가가 가능한 상하 치령의 차이가 III급 부정교합의 조기 징후로 개연성이 있다면, 이것은 조기 감별진단의 한 도구로써 가치가 있을 것으로 판단되었다. 부산대학교치과병원 소아치과에 내원한 Hellman 치령 IIA, IIC의 환자 를 대상으로, 측두방사선사진, 파노라마 방사선사진, 석고 모형과 임상사진을 조사하여 정상교합군과 전치부 반대교합군 각 50명을 선택하였다. 이들의 파노라마 방사선사진을 이용하여 Demirjian법으로 각 군의 상하 치령 및 제1대구치 맹출율을 구하고 그 차이를 비교, 분석하여 다음과 같은 결과를 얻었다. 1. 정상교합군과 반대교합군 모두에서 역령에 비해 치령이 높게 나타났으며 성차는 보이지 않았다(p>0.05). 2. 정상교합군과 반대교합군의 상하 치령의 차이는 각각 0.22세와 0.69세로 반대교합군에서 더 큰 차이를 보였다(p<0.05). 3. 정상교합군에 비해 반대교합군에서 상하 제1대구치 맹출율의 차이가 크게 나타났다(p<0.05). It was easy to find that children of a skeletal anterior crossbite in the early mixed dentition period showed a stark difference in the dental maturity between their maxillary and mandibular teeth, if they have stronger physical characteristics. If the difference of dental age between maxillary and mandibular teeth which can be identified via panoramic radiographs may serve as an early sign of class III malocclusion, this is considered valuable as a tool of early detection diagnosis. We obtained lateral cephalometric radiographs, panoramic radiographs, working model and clinical images of patients of Hellman dental age IIA and IIC who visited the department of pediatric dentistry, Pusan National University Dental Hospital and examined them to select 50 patents for normal occlusion group and skeletal anterior crossbite group, respectively. Their panoramic radiographs were used for the Demirjian's method to figure out dental ages of maxillary and mandibular teeth of each group and the eruption rate of the first molars. Their differences are as follows: 1. In both groups, the dental ages from Demirjian's method were advanced than the chronological ages. No sexual dimorphism was detected for the chronological or dental age in either group (p>0.05). 2. The difference of dental age of maxillary and mandibular teeth between the normal occlusion group and crossbite group was 0.22 and 0.69 years, respectively, with a higher difference in crossbite group(p<0.05). 3. Compared to the normal occlusion group, the crossbite group showed a higher difference in the eruption rate between maxillary and mandibular first molar(p<0.05).

사인에 의한 즉시형 과민반응의 억제 효과

신태용,염정열,김원,김현미,박해정,장진이,조성룡,채병숙 우석대학교 생명과학연구소 1998 생명과학연구소 논문집 Vol.2 No.-

We investigated the effect of aqueous extract of Ammomum xanthioides(AXAE) on immediate hypersensitivity. AXAE inhibited systemic anaphylaxis induced by compound 48/80 in mice. AXAE inhibited serum histamine levels induced by compound 48/80 in mice. Moreover, AXAE dose-dependently inhibited histamine release in peritoneal mast cells activated by compound 48/80. These results indicate that the AXAE may be benificial in the regulation of immediate type allergic reaction.

직연신 방사과정에서의 폴리프로필렌 구조해석 : 분자량 및 권취속도에 따른 영향 Effect of Molecular Weight and Take-up Velocity

신현세,이태균 단국대학교 1998 論文集 Vol.33 No.-

Abstrack : The development of the structure of polypropylene fibers during the direct drawing melt spinning process was analyzed by comparing fibers which have gone through the whole direct drawing melt spinning process and fibers in which the drawing step was omitted. The relative contribution of the drawing step was evaluated so that the information on the stages of development of the structure can be utilized as the foundation for future research on functional or high performance fibers. Four different molecular weights and take­up speeds were utilized to study the effect of these variables on the development of the structure. The samples taken up omitting the drawing step were designated as undrawn fibers and the fibers in which the drawing was carried out as drawn fibers. When the properties of the drawn fibers were taken as reference and the degree of structure formation in the undrawn fibers described by the percentage therof, 99, 85 and 95% of the density crystallinity and crystalline orientation had already developed prior to the drawing step. In the other hand the overall orientation was found to be more dependent on the drawing process, with about 64-73% of the orientation being developed in the drawing step and 26.8-36.1% being developed prior to the drawing step. The amorphous orientation of the undrawn fibers exhibited a negative value suggesting that the rotation of lamella to the final structure occurs in the drawing step. The highest tenacity obtained in this experiment was 4.5g/den and the highest modulus 25.4g/den when PP of Mn=230,000 was spun at 800m/min.

Streptomyces 속 균주 HA-40이 생산하는 내열성 α-Amylase의 효소학적 성질

신운섭,이동희,이현우,권태종,정호권 建國大學校 附設 産業技術硏究所 1988 논문집 Vol.13 No.-

토양으로부터 분리한 Streptomyces 속 균주 HA-40이 생산하는 내열성 α-amylase의 효소학적 성질을 조사한 결과는 다음과 같다. 효소의 작용 최적 온도는 65℃였으며 activation energy 는 16.9Kcal/mole이었고 60℃에서는 안정하였으나 70℃에서는 30분 처리로 약 50%, 1시간 처리로 약 75%가 실활하였다. 그러나 ??를 0.1mM농도로 첨가한 경우엔 70℃ 에서 1시간 처리하여도 잔존활성도가 55%이상으로 열변성에 대한 ??의 보호효과가 있었다. 본 효소는 pH7에서 활성이 가장 강하였으며 안정성도 가장 높았다. 그리고 금속이온 중에서 ??의 첨가로 효소활성이 증대되었으며 ??, ??, ??등에 의해서 활성이 저해되었다. 각종 효소저해제는 대부분 영향을 미치지 못하였으나 EDTA가 반응액 중에 공존하면 효소활성이 강하게 저해되었다 본 효소는 maltotriose이하의 당류에 대해서는 분해력이 없었으며 amylose에 대한 환원당 생성력을 100으로 하였을때 soluble starch 108, amylopectin 94, glycogen 87, dextrin 48, potato starch 25, corn starch 18로 생전분에 대한 분해력은 soluble starch에 비해서 1/4∼1/5 정도밖에 되지 않았다. 그리고 soluble starch에 대한 Km 치는 1.9㎎/㎖였다. The properties of a thermostale α-amylase produced from Streptomyces sp. strain HA-40, isolated from soil, were investigated. The optimal temperature of the enzyme was 65℃ and the enzyme was stable below 60℃. However, at 70℃ about 75% of the activity was lost after 1hr treatment, but by the addition of ?? in the concentration of 0.1mM only 45% of the activity was lost. The enzyme was most active and stable at around pH 7. The activity of the enzyme was increased by the addition of ??, but depressed by ??, ??, and EDTA. The enzyme could be hydrolyze soluble starch, amylose, amylopectin and glycogen well, and could also digest dextrin and raw starch. The Km value for soluble starch was 1.9㎎/㎖.

Vincristine Sulfate가 Mouse 모낭세포의 미세구종에 미치는 영향에 관한 전자현미경적 연구

신현태,이규식,류재만 한양대학교 의과대학 1990 한양의대 학술지 Vol.10 No.1

Vincristine sulfate, one of thte Vinca alkaloids has been used clinically as an antitumor reagent. Vincristine sulfate has high affinity to the basic protein subunit of microtubules, that it disrupts the mitotic spindle apparatus. As a result this reagent causes the cell to arrest in metaphase and to die finally. When vincristine was spread on the skin it would damage the growth of the hair, that the author undertook the present study to observe the effect of vincristine onthe cytoplasmic organelles of the hair follicular cells. Male albino mouse, DDY strain, weighing 20gm were used as an experimental animals. The back skin of experimental animals were spread with a vincristine sulfate solution(1mg/5ml) once a day for 6 days after cutting of the hair. The experimental animals were sacrificed at 2 day, 4 day, and 6 day after first treatmetn and specimens obtained from the skin of the vincristine treated area. The specimens were prefixed in 2% glutaradehyde-2.5% paraformaldehyde solution and postfixed in 1% osmium tetroxide solution and then ultrathin section, 600-800A, were made and stained with uranyl acetate and lead citrate. Some specimens were also fixed in 10% formalin for hematoxylin eosin stain. The specimens were observed with light microscopy and JEM 100cx-II electrom microscopy. The results were as follows. 1. Hair follicles were decreased numerously in the skin of the 4th and 6th vincristine treated group. And the length of the hair was reduced in the skin of the 6th vincristine treated group. 2. In the 2nd vincristine treated group, irregularly shaped nuclei, reduced heterochromatin and numerous vacuoles wre observed in the external root sheath cells. In cells of the inner root sheath, heterochromatin was segregated and clumpped, and cristae of mitochondria was disappeared. 3. In the 4th vincristine treated group, dilatation of cisternae of the rough endoplasmic reticulum, degenerated mitochondria were observed in the external root sheath cells. And trichohyaline granules were reduced and condensed nuclei were observed in the cells of the Henley's layer. 4. In the 6th vincristine treated group, cuticle cells were more keratinized. And in the external root sheath cells, colummar shaped nuclei, degenerative mitochondria and rough endoplasmic reticulum, and decreased cisternae of Golgi apparatus were observed. Consequently, it is suggested that vincristine sulfute would induce the degenerative changes in the hair follicular cells.