Felodipine 함유 Eudragit microsphere의 생체이용율

김남순,김현주,김영일,양재헌 우석대학교 생명과학연구소 1998 생명과학연구소 논문집 Vol.2 No.-

Microencapsulations of felodipine which is one of the calcium channal blocker, using the mixture of Eudragit RL®, L® and E® and celullose on the base of Eudragit RS® were investigated. The average diameters of microspheres were from 40 to 55㎛. The incorporation ratios of felodipine into the microspheres were higher than 90 %. After 7h, about 60 % of felodipine was released. Dissolution rate of felodipine from microsperes was increased by the addition of Cremopher®. After oral administration of microspheres, the Cmax were reduced and incresed the bioavailities as up to 50 %.

비소 중독된 흰쥐의 신장에 미치는 팔진탕의 영향

임종필,서은실,김훈 우석대학교 생명과학연구소 1998 생명과학연구소 논문집 Vol.2 No.-

Sodium arsenate and Paljin-Tang extract, a herbal restorative were treated p. o. 20 ㎎/㎏ and 500 ㎎/㎏, respectively, and concurrently to rats, and examined the variation of kidney weight, the accumulation of arsenic and effects of metabolic exzymes in kidney of rats. The results obtained in this study were as follows: 1. The ratio of kidney weight/ body weight of arsenic-treated group increased but the group of concurrent administration with PJT showed significant decrease. 2. The accumulation of arsenic in kidney of arsenic-treated group increased but the group of concurrent administration with PJT showed significant decrease. 3. The value of cytochrome P-450 and NADPH-cytochrome c reductase activity showed no variance, but that of protein increased in arsenic-treated group but concurrent administration with PJT showed significant recovery from the toxicity of arsenic in kidney.

Cefradine 함유 alginate bead의 제조 및 용출

김대섭,구자성,양재헌 우석대학교 생명과학연구소 1998 생명과학연구소 논문집 Vol.2 No.-

Cefradine, first generation cephalosporins, is a water soluble antibiotic agent. We prepared the alginate beads containing cefradine for developing release and evaluated the dissolutions of cefradine from beads. Cefradine was encapsulated into inner water phase of W/O/W emulsion beads which were prepared by cross-linking of alginate with Ca^2+. The delayed release patterns of cefradine for W/O/W emulsion beads were observed as it was compared with alginate simple beads without oil phase. As a results, W/O/W multiple emulsion beads could be use a controlled release DDS of water soluble agents such as cefradine. Also chitosan of high M.W could be a good intermediator of controlled release DDS.

단삼이 마우스 T-lymphocyte 및 Macrophage에 미치는 영향

은재순,엄승렬,염정열,전훈 우석대학교 생명과학연구소 1998 생명과학연구소 논문집 Vol.2 No.-

The purpose of this research was to investigate the regulative action of water extract of Salviae miltiorrhizae Radix(SE) on thymocyte and macrophage in mice. The proliferation in thymocyte was tested using a colorimetric tetrazolium assay(MTT assay), The apoptosis and subpopulation in thymocyte were tested using a flow cytometry. Nitric oxide(No) production from mouse peritoneal macrophage was tested using a Griess reagents and the phagocytic activity was tested using a lucigenin chemiluminescence. SE did not affect the proliferation, apoptosis and subpopulation(CD4^+/CD8^+) in thymocyte in vivo, but enhanced the proliferation in thymocyte in vitro. The n-BuOH fraction and water fraction of SE suppressed the nitric oxide production and results suggest the SE does not affect the activity of thymocyte, but suppressed the activity of macrophage.

일부 여대생의 식이섬유 섭취와 체지방 함량에 관한 연구

주은정 우석대학교 생명과학연구소 1998 생명과학연구소 논문집 Vol.2 No.-

This study was conducted to investigate dietary fiber intake, body fat content, and their correlation in 30 female college students living in rural. The nutritional status was analyzed using 3-day dietary record, venous blood sampling and fatness analyzer(Futrex-6000). The mean values for BMI, hemoglobin, hematocrit, triceps skinfold, and body fat content of the subjects were 19.44, 11.61g/㎗, 34.83%, 16.4㎜, and 23.86%, respectively. Daily intakes of energy, protein, crude fiber, and dietary fiber were 1571.5kcal, 58.91g, 4.80g, and 14.68g respectively. Dietary fiber intake was positively correlated with energy, protein, carbohydrate, vitamin C, and crude fiber intakes. The most significant correlation was observed between dietary fiber intake and energy intake(r=0.719, p<0.001). Correlations between dietary fiber intake and intakes of major food groups such as cereals, fruits, and seaweeds were significant. The most significant correlation(r=0.767, p<0.001) between dietary fiber intake and intakes of seaweeds was observed. There was no significant relationship between dietary fiber intake and body fat content. However, there were significant negative correlation between crude fiber intake and lean body mass(r=-0.407, p<0.05).

靈樞·百病始生篇에 대한 各生註의 比較硏究 : 隋唐에서 明代까지

陸相元,姜庚來 우석대학교 생명과학연구소 1998 생명과학연구소 논문집 Vol.2 No.-

베타카로틴 알지네이트 비드의 용출

고광묵,김영일,양재헌 우석대학교 생명과학연구소 1998 생명과학연구소 논문집 Vol.2 No.-

We prepared alginate beads containing β-carotene which could be a gel at low pH and could be swell at weak alkali in order to protect the degradation of β-carotene in the stomach. β-carotene was incorporated into the beads up to 95 %. The release rate of β-carotene was affected by the concentration of Calcium chloride, contents of β-carotene and surfactants. When β-Carotene incorporated into alginate beads it could be delivered in the intestine.

구루텐을 사용해 제조한 새알심의 물성

이태규,김종화 우석대학교 생명과학연구소 1998 생명과학연구소 논문집 Vol.2 No.-

Adzuki-bean porridges containing Sealsim made of gluten and wet grinded glutinious rice were produced and sterilized under condition of 120℃ for 20 min. This study examined rheological properties of Sealsim in adzuki-bean porridge under room temperature for 35 days. The results summarized as follows. Sealsim made by mixture 1 without arrowroot starch showed better quality than that made by mixture 2 containing arrowroot starch. Sealsim dried under 100℃ for 20 min. showed better quality for early 5 days. But the quality was decreasing continuously and became similiar after 30 days.

Chitosan 과 ionized calcium 정제의 용출 특성에 관한 연구

장윤정,남용옥,양재헌 우석대학교 생명과학연구소 1998 생명과학연구소 논문집 Vol.2 No.-

Recently, chitosan has being focused on not only the role of a potential and natural resource with antibacterial or antifungal effect but also the natural controller of releasing of drugs or principle actives. Calcium is one of the most important minerals of human that concerned with the intra- or inter-cellular function, But ionized calcium, prepared from shell through the condition of high temperature, own the antibacterial effects. We prepared the mixed tablet of chitosan and ionized calcium with several additives such as starch paste, ethylcellulose(EC), hydroxyprophyl methyl-celullose(HPMC) or hydroxyprophylcellulose(HPC). Each formulation with a additive was adopted as a challenger to modify of release profiles of calcium. Finally following both cationic properties, chitosan & ionized calcium, the mucoadhesive effects in the oral cavity are favorable to introduce for chewable tablet.

GABA agonist와 antagonist의 주입이 닭의 monocular Optokinetic nystagmus(OKN)에 미치는 영향

김명순 우석대학교 생명과학연구소 1998 생명과학연구소 논문집 Vol.2 No.-

Chicken monocular head and eye optokinetic nytagmus(OKN) were observed by coil recording after intravitreal administration of GABA agonist(THIP) and antagonist (SR95531) into the open and closed eye. Before injection of drugs the chicken displayed an OKN for T-N stimulation being more efficient in evoking this visuomotor reflex than N-T stimulation. The injection of GABA agonist(THIP) into either the open or closed eye induced a decrease of the head and eye OKN. On the other hand, the injection of GABA antagonist(SR 95531) into the closed eye, the head and eye OKN evoked an increase whereas the injection of GABA antagonist(SR 95531) into the open eye, the head and eye OKN provoked a decrease or disappearance. Thus, GABA antagonist abolished the directional asymmetry of the head and eye OKN, indicating the involvement of GABAergic mechanisms in the inhibition of the N-T component of the monocular OKN.