Poster Session:PS 0548 ; Nephrology : Onodera`s Prognostic Nutritional Index May be a Signifi cant Nutritional Factor in Renal Transplantation Patients According to the Presence or Absence of Chronic Kidney Disease

( Gyoung Hoon Kang ),( Jiin Kang ),( Ye Na Kim ),( Ho Sik Shin ),( Yeon Soon Jung ),( Hark Rim ),( Hyun Yul Rhew ) 대한내과학회 2014 대한내과학회 추계학술대회 Vol.2014 No.1

Background: Evaluation of nutritional status is essential clinical procedures for managing renal transplantation patients, especially in status of chronic kidney disease (CKD). However, no standard method for assessing the nutritional status in renal transplantation patients exists. Onodera`s Prognostic Nutritional Index (OPNI) is a method that considers serum albumin level and total lymphocyte count. This simple method may involve common measures and can be applied rapidly in a large number of patients. Validation of OPNI has been performed for patients with end-stage liver disease, active tuberculosis, and gastrointestinal malignancies. Methods: We examined the OPNI scores of 184 renal transplantation patients (50.2 ± 11.3 years; 115 men and 69 women). The OPNI is calculated based on the serum albumin level and total lymphocyte count and uses the following equation: OPNI = 10 × serum albumin (g/dL) + 0.005 × total lymphocyte count (/mL). Logistic regression analysis was performed for predicting malnutrition in renal transplantation patients. Results: The average OPNI value was 48.9 ± 7.1, and OPNI values were normally distributed. According to logistic regression for predicting malnutrition, male, total lymphocyte count and chronic kidney disease predicted malnutrition. Conclusions: These results suggest that OPNI may be a signifi cant nutritional marker in renal transplantation patients. The simple OPNI method is a clinically useful marker for the assessment of nutritional status in renal transplantation patients.

+61A>G polymorphism in the <i>EGF</i> gene does not increase the risk of lung cancer

KANG, Hyo-Gyoung,CHOI, Jin Eun,LEE, Won Kee,KAM, Sin,CHA, Sung Ick,KIM, Chang Ho,JUNG, Tae Hoon,PARK, Jae Yong Blackwell Publishing Asia 2007 Respirology Vol.12 No.6

<P>Background and objectives: </P><P>Epidermal growth factor (EGF) plays an important role in tumourigenesis by binding with its receptor, EGFR. Variations in the DNA sequence in the <I>EGF</I> gene can lead to an alteration in EGF production and/or activity, which can affect an individual's susceptibility to lung cancer. To test this hypothesis, this study examined the association between the +61 A>G polymorphism in the 5′-untranslated region of the <I>EGF</I> gene and the risk of lung cancer in a Korean population.</P><P>Methods: </P><P>The <I>EGF</I>+61 A>G genotype was determined in 432 lung cancer patients and 432 healthy age- and gender-matched control subjects.</P><P>Results: </P><P>The +61 AA and +61 AG genotypes were not significantly associated with the risk of lung cancer compared with the +61 GG genotype (adjusted OR = 1.02, 95% CI: 0.77–1.37; and adjusted OR = 0.81, 95% CI: 0.51–1.29, respectively). In addition to the reference model, the <I>EGF</I>+61 A>G polymorphism had no significant association with the risk of lung cancer under both dominant and recessive models for the +61A allele (adjusted OR = 0.98, 95% CI: 0.74–1.29; and adjusted OR = 0.80, 95% CI: 0.51–1.24, respectively).</P><P>Conclusion: </P><P>These results suggest that the <I>EGF</I>+61 A>G polymorphism may not significantly affect the susceptibility to lung cancer in the Korean population.</P>

Effect of water temperature on embryonic development and larval survival of an intertidal snail, Nassarius festivus (Powys, 1835)

Kang, Sin-Kil,Sung, Chan-Gyoung,Chung, Jiwoong,Park, Dong-Ho,Lee, Jong-Hyeon,Lee, Chang-Hoon The Malacological Society of Korea 2016 The Korean Journal of Malacology Vol.32 No.1

This study was carried out to determine the optimal water temperature for the embryonic development and laboratory culture of larvae of an intertidal mud snail, Nassarius festivus. The embryos and hatched veliger larvae of N. festivus were incubated at six different temperatures (5, 10, 15, 20, 25 and $30^{\circ}C$). Developmental time for each stage decreased as water temperature increased. The elapsed time to develop to the veliger larva at 15, 20, 25 and $30^{\circ}C$ was 559, 155, 131 and 103 hrs, respectively. At 5 and $10^{\circ}C$, embryo developed to veliger larvae but failed to hatch out of the egg capsule. In contrast, all embryos successfully hatched in the temperature range from 15 to $30^{\circ}C$. The biological minimum temperature during the embryonic development of N. festivus was estimated to be $9.5{\pm}0.4^{\circ}C$. The cumulative water temperatures for blastula, gastrula and veliger stages were calculated as $111{\pm}84$, $486{\pm}185$, $1,164{\pm}72^{\circ}C$, respectively. Temperature also affected the larval survival. Five days after hatching, more than 84% of larvae survived at all experimental temperatures. However, survival began to decrease after 6 days. It was 0% at $30^{\circ}C$. Survival of larvae incubated for 8 days was higher at 15 and $20^{\circ}C$ than other experimental temperatures. We therefore suggest that the optimal range of temperature for embryonic development and larval survival of N. festivus is $15-20^{\circ}C$.

Detection of EROD (ethoxyresorufin-O-deethylase) activity using the embryo of olive flounder, Paralichthys olivaceus

Chan-Gyoung Sung(성찬경),Chang-Hoon Lee(이창훈),Ji-Hye Lee(이지혜),Seong-Dae Moon(문성대),Sin-Kil Kang(강신길),Jong-Hyun Lee(이종현) 환경독성보건학회 2012 한국독성학회 심포지움 및 학술발표회 Vol.2012 No.10

한국인 원인 불명 조기 난소부전 환자에서 Inhibin-α 유전자+769 G/A돌연변이 유무

이경훈 ( Gyoung Hoon Lee ),최영민 ( Young Min Choi ),김종미 ( Jong Mi Kim ),김진주 ( Jin Ju Kim ),황규리 ( Kyu Ri Hwang ),구승엽 ( Seung Yup Ku ),이규섭 ( Kyu Sup Lee ),강병문 ( Byung Moon Kang ),윤병구 ( Byung Koo Yoon ),한명석 ( 대한산부인과학회 2006 Obstetrics & Gynecology Science Vol.49 No.8

목적: 한국인 원인 불명 조기 난소부전 환자들을 대상으로 inhibin-α 유전자내 +769 G/A 돌연변이의 빈도를 파악하고자 하였다. 연구 방법: 40세 이전에 무월경과 1개월 간격으로 검사한 두 번의 난포자극호르몬 수치가 상승되어 있으며 다른 수술적 처치나 약물 요법, 면역 질환의 과거력이 없는 조기 난소부전 환자 100명을 대상으로 하였으며, inhibin-α 유전자내의 +769 G/A 돌연 변이 양상을 PCR 및 BbvI 제한효소를 이용한 RFLP를 시행하였다. 결과: 조기 난소부전 환자 100명을 모두에서 inhibin-α 유전자의 +769 G/A 돌연 변이를 발견할 수 없었다 (0/100=0.0%). 결론: 한국인 조기 난소부전 여성에서 inhibin-α 유전자의 +769 G/A 변이는 발견되지 않았다. Objective: To evaluate the incidence of the +769 G/A mutation of inhibin-α gene in Korean patients with idiopathic premature ovarian failure. Design: DNA analysis of the mutation. Methods: One hundred patients with idiopathic premature ovarian failure (POF) were recruited. Patient with known causes of premature ovarian failure were excluded: cytogenetic abnormalities, prior chemotherapy, prior bilateral oophorectomy and autoimmune disease, etc. DNA was extracted from peripheral blood and the +769 G/A variant of Inhibin-α gene (INH- α) was analyzed by polymerase chain reaction (PCR) and restriction fragment length polymorphism using restriction enzyme BbvI . Results: We didn`t find any case of +769 G/A variant in Inhibin-α gene in 100 Korean patients with idiopathic POF. Conclusion: This result suggests that in contrary to the recent data reported on the Asian population, the +769 G/A variant in inhibin-α gene may not exist in Korean patients with idiopathic POF.

Change of electrical properties of (K0.5Na0.5) (Mn0.005Nb0.995)O3 thin films induced by gamma-ray irradiation

Byung Hoon Kim,Sun A Yang,Sin Wook Kang,Gi Ppeum Choi,Sam Yeon Cho,한진규,Gyoung Ja Lee,이민구,Hae Jin Seog,Ill Won Kim,Sang Don Bu 한국물리학회 2016 Current Applied Physics Vol.16 No.5

We investigated the effects of gamma-ray (g) irradiation on the electrical properties of (K0.5Na0.5) (Mn0.005Nb0.995)O3 (KNMN) thin films. The KNMN thin films were prepared on Pt/Ti/SiO2/Si substrate using a chemical solution deposition method through a spin-coating process and were subject to g radiation at various total doses from 0 to 3000 kGy. The structural properties as well as the ferroelectric and dielectric properties of the prepared films were examined before and after g irradiation. We found that their crystalline quality did not vary significantly with an increase in the total dose. It was also observed that the remnant polarization value of the films decreased by ~10%, but the films maintained ferroelectricity even after irradiation up to 3000 kGy. In addition, the dielectric constant of the films decreased with the total dose. The observed variation of the electrical properties on the total dose might be mainly associated with the mobile defects in Mn-doped KNN thin films such oxygen vacancy and the stored energy gained from gamma-rays.

압출쌀가루의 이화학적 특성 및 밀가루 대체 쿠키 특성

위경진 ( Gyoung Jin We ),이인애 ( In Ae Lee ),강태영 ( Tae Young Kang ),민주홍 ( Joo Hong Min ),강위수 ( Wie Soo Kang ),고상훈 ( Sang Hoon Ko ) 한국산업식품공학회 2011 산업 식품공학 Vol.15 No.4

The purpose of this study is to prepare extruded rice flours suitable for baking rice cookies. The extruded rice flours were prepared at 100 and 130oC temperature and 25 and 27% moisture content in a co-rotating twin screw extruder. The rice extrudates were dried at 100oC for 18 hr and subsequently ground into the fine flour. Characteristics of the extruded rice flours were examined by rapid visco analysis, hydration property analysis, differential scanning calorimetry (DSC), and in vitro digestion test. Water absorption, solubility, and swelling power of all extruded rice flours were higher than those of native rice flour. DSC analysis showed that native rice flour had a peak at about 65oC while all extruded rice flours did not show any peaks since they were already gelatinized during the extrusion process. Viscosity of the extruded rice flours decreased with increasing temperature and lowering moisture content in the extrusion process. The extruded rice flours prepared at 130oC exhibited lower viscosity than those prepared at 100oC. The operating temperature of the extrusion process was critical for the starch digestion in vitro. The extruded rice flours prepared at 130oC showed a rapid decrease in digestible starch content while an increased level of slowly digestible starch content was observed compared to those treated at 100oC in the extruder. Cookies were prepared with a mixture of wheat flour and extruded rice flours at the ratio of 7 to 3. The cookies made with the extruded rice flours had lower spread factor and darker yellow color than those prepared with wheat flour only. Hardness of the extruded rice flour-added cookies was similar to that of the wheat flour cookie whereas their overall acceptance was better. Therefore the rice cookies partially supplemented with extruded rice flours may have a potential as early childhood foods which require soft texture and allergy reduction.

임계지역 석회암의 일축압축강도와 점하중강도지수의 상관관계 연구

김경만(Gyoung Man Kim),김대훈(Dae Hoon Kim),강중석(Jung Seock Kang),강상수(Sang Soo Kang),백환조(Hwanjo Baek) 한국암반공학회 2012 터널과지하공간 Vol.22 No.5

일축압축강도(UCS)는 암석의 주요 물성 중 하나이다. 그러나 항상 UCS 실험을 위한 시료를 취득할 수는 없다. 이러한 경우 점하중시험과 같은 간접시험법이 유용한 대안이 될 수 있다. 본 연구에서는 임계지역에 분포하는 석회암을 대상으로 일축압축강도와 점하중강도지수 사이의 상관관계를 분석하였으며 선형 회귀식이 제안되었다. 또한 석회암을 대상으로 수행된 이전 연구결과와 이번 연구 결과를 비교하였다. 동일한 암종이라도 국내에서 분포하는 지역에 따라 변환계수가 다를 수 있으며, 분석의 정확성을 높이기 위해서는 더 많은 자료가 필요할 것으로 판단된다. Though the uniaxial compressive strength (UCS) of rock material is one of the key properties for rockmass characterization purposes, core samples for the test cannot always be obtained from the field. Indirect tests such as the point load test (PLT) can be a useful alternative. In this study, correlation between the UCS and the point load strength index of limestone of Imgye area was analyzed, and the linear regression equation obtained from regression analysis of two variables was suggested. The results of this study were also compared with previous studies on limestone. It was suggested that conversion factors for the same rock type from diverse areas in Korea may have different values, and more data should be obtained to increase the accuracy of regression analysis.

브레이크 캘리퍼 내부 검사를 위한 비전시스템 개발

권경훈 ( Gyoung Hoon Kwon ),추형곤 ( Hyung Gon Chu ),김진영 ( Jin Young Kim ),강준희 ( Joonhee Kang ) 한국센서학회 2017 센서학회지 Vol.26 No.1

Development of vision system as a nondestructive evaluation system can be very useful in screening the defective mechanical parts before they are assembled into the final product. Since the tens of thousands of the mechanical parts are used in an automobile carefully inspecting the quality of the mechanical parts is very important to maximize the performance of the automobile. To sort out the defective mechanical parts before they are assembled, auto parts fabrication companies employ various inspection systems. Nondestructive evaluation systems are getting rapidly popular among various inspection systems. In this study, we have developed a vision system to inspect the inside of the brake caliper, a part that is used to compose a brake which is the most important to the safety of the drivers and the passengers. In a brake caliper, a piston is pushed against the brake disk by oil pressure, causing a friction to damp the rotation of the wheel. Inside the caliper, a groove is positioned to adopt an oil seal to prevent the oil leaks. Inspecting the groove with our vision system, we could examine the existence of the contaminants which are normally the residual tiny pieces from the machining process. We used a high resolution GigE camera, 360 degree lens to look in the inside view of the caliper at once, and a special illumination system in this vision system. We used the edge detection technique to successfully detect the contaminants which were in the form of small metal chips. Labview graphical program was used to process the digital data from the camera and to display the vision and the statistics of the contaminants. We were very successful in detecting the contaminants from the various size calipers. We think we are ready to employ this vision system to the caliper production factories.

Genetic Variants in Histone Modification Regions are Associated with the Prognosis of Lung Adenocarcinoma

( Yong Hoon Lee ),( Jae Yong Park ),( Hyo-Gyoung Kang ),( Shin Yup Lee ),( Jin Eun Choi ),( Sook Kyung Do ),( Mi Jeong Hong ),( Jang Hyuck Lee ),( Ji Yun Jeong ),( Young Woo Do ),( Eung Bae Lee ),( Ky 대한결핵 및 호흡기학회 2021 대한결핵 및 호흡기학회 추계학술대회 초록집 Vol.129 No.0

We investigated the association between genetic variants in the histone modification regions and the prognosis of lung adenocarcinoma after curative surgery. Potentially functional SNPs were selected using integrated analysis of ChIP-seq and RNA-seq. The SNPs were analyzed in a discovery set (n=166) and a validation set (n=238). The associations of the SNPs with overall survival (OS) and disease-free survival (DFS) were analyzed. A total of 279 SNPs were selected for genotyping. Among these, CAPN1 rs17583C>T was significantly associated with better OS and DFS (P = 0.001 and P = 0.007, respectively), and LINC00959 rs4751162A>G was significantly associated with worse DFS (P = 0.008). Luciferase assays showed a significantly lower promoter activity of CAPN1 in the rs17583 T allele than C allele (P = 0.008), and consistently the CT+TT genotypes had significantly lower CAPN1 expression than CC genotype (P = 0.01) in clinical samples. The rs4751162 G allele had higher promoter activity of GLRX3 than A allele (P = 0.05). The motif analyses and ChIP-qPCR confirmed that the variants are located in the active promoter/enhancer regions where transcription factor binding occurs. This study showed that genetic variants in the histone modification regions could predict the prognosis of lung adenocarcinoma after surgery.