Quantitative Assay for the Binding of Jun-Fos Dimer and Activator Protein-1 Site

Lee, Sang-Kyou,Park, Se-Yeon,Jun, Gyo,Hahm, Eun-Ryeong,Lee, Dug-Keun,Yang, Chul-Hak Korean Society for Biochemistry and Molecular Biol 1999 Journal of biochemistry and molecular biology Vol.32 No.6

The Jun and Fos families of eukaryotic transcription factors form heterodimers capable of binding to their cognate DNA enhancer elements. We are interested in searching for inhibitors or antagonists of the binding of the Jun-Fos heterodimer to the activator protein-1 (AP-1) site. The basic-region leucine zipper (bZIP) domain of c-Fos was expressed as a fusion protein with glutathione S-transferase, and allowed to form a heterodimer with the bZIP domain of c-Jun. The heterodimer was bound to glutathione-agarose, to which were added radiolabeled AP-1 nucleotides. After thorough washing, the gel-bound radioactivity was counted. The assay is faster than the coventional electrophoretic mobility shift assay because the gel electrophoresis step and the autoradiography step are eliminated. Moreover, the assay is very sensitive, allowing the detection of picomolar quantities of nucleotides, and is not affected by up to 50% dimethylsulfoxide, a solvent for hydrophobic inhibitors. Curcumin and dihydroguaiaretic acid, recently known inhibitors of Jun-Fos-DNA complex formation, were applied to this Jun-GST-fused Fos system and revealed to decrease the dimer-DNA binding.

Quantitative Assay for the Binding of Jun-Fos Dimer and Activator Protein-1 Site

Yang, Chul Hak,Jun, Gyo,Lee, Dug Keun,Lee, Sang Kyou,Park, Se Yeon,Hahm, Eun Ryeong 생화학분자생물학회 1998 BMB Reports Vol.32 No.6

The Jun and Fos families of eukaryotic transcription factors form heterodimers capable of binding to their cognate DNA enhancer elements. We are interested in searching for inhibitors or antagonists of the binding of the Jun-Fos heterodimer to the activator protein-1 (AP-1) site. The basic-region leucine zipper (bZIP) domain of c-Fos was expressed as a fusion protein with glutathione S-transferase, and allowed to form a heterodimer with the bZIP domain of c-Jun. The heterodimer was bound to glutathione-agarose, to which were added radiolabeled AP-1 nucleotides. After thorough washing, the gel-bound radioactivity was counted. The assay is faster than the conventional electrophoretic mobility shift assay because the gel electrophoresis step and the autoradiography step are eliminated. Moreover, the assay is very sensitive, allowing the detection of picomolar quantities of nucleotides, and is not affected by up to 50% dimethylsulfoxide, a solvent for hydrophobic inhibitors. Curcumin and dihydroguaiaretic acid, recently known inhibitors of Jun-Fos-DNA complex formation, were applied to this Jun-GST-fused Fos system and revealed to decrease the dimer-DNA binding.

Quantitative Assay for the Binding of Jun-Fos Dimer and Activator Protein-1 Site

Yang,Chul-Hak,Jun,Gyo,Hahm,Eun-Ryeong,Lee,Sangkyou,Park,Seyeon,Lee,Dug-Keun The Korea Science and Technology Center 1999 BMB Reports Vol.32 No.6

The Jun and Fos families of eukaryotic transcription factors form heterodimers capable of binding to their cognate DNA enhancer elements. We are interested in searching for inhibitors or antagonists of the binding of the Jun-Fos heterodimer to the activator protein-1 (AP-1) site. The basic-region leucine zipper (bZIP) domain of c-Fos was expressed as a fusion protein with glutathione S-transferase, and allowed to form a heterodimer with the bZIP domain of c-Jun. The heterodimer was bound to glutathione-agarose, to which were added radiolabeled AP-1 uncleotides. After thorough washing, the gel-bound radioactivity was counted. The assay is faster than the coventional electrophoretic mobility shift assay because the gel electrophoresis step and the autoradiography step are eliminated. Moreover, the assay is very sensitive, allowing the detection of picomolar quantities of nucleotides, and is not affected by up to 50% dimethylsulfoxide, a solvent for hydrophobic inhibitors. Curcumin and dihydroguaiaretic acid, recently known inhibitors of Jun-Fos-DNA complex formation, were applied to this Jun-GST-fused Fos system and revealed to decrease the dimer-DNA binding.

An Investigation on the Life of Master Won Guk Lee, the Creator of Chung-Do-Kwan

( Seon Young Jung ),( Jun Ho Lee ),( Seung Woo Shin ),( Chun Hwan Cho ),( Seung Hyun Jang ),( Won Shin ) 경남대학교 기초과학연구소 2014 기초과학지 Vol.31 No.-

The purpose of this study is to review (or, investigate) the life of Won Guk Lee. He is the creator of Chung-Do-Kwan, which is one of the five original Taekwondo kwans. Data was gathered from advanced research, newspapers, documentaries, the Internet, magazines, and journals etc. Thus, we were able to create a sequence of events based on the research data. First, we can say that in Won Guk Lee``s career, he created Chung-Do-Kwan and brought Dangsoodo to Korea. These events have had a significant development on Taekwondo. Second, Taekwondo curriculum, which was published by Won Guk Lee, established the foundation of Taekwondo poomsae, as well as giving us the pure spirit of Korean martial arts.

고요산혈증의 위험인자 : 아산시 지역 주민을 대상으로

김용배,장원기,황보영,김화성,안규동,이병국,이용진,이종은,이준숙,이찬구,이한정,이성수 순천향대학교 2006 Journal of Soonchunhyang Medical Science Vol.12 No.1

Objective : Serum uric acid has now been identified as a marker for a number of metabolic and hemodynamic abnormalities. The aim of this study is to evaluate risk factors which influence hyperuricemia in the citizens of Asan. Method : The study design was cross-sectional and research subjects were 1086 citizens of Asan. General characteristics, cigarette smoking, alcohol consumption, and past history were obtained by interview using a structural questionnaire. Also, we measured height, weight and blood pressure, and serum uric acid of the subjects. Results : The mean serum uric acid level was 5.27 ㎎/㎗ in men and 4.01 ㎎/㎗, in women, In the multiple logistic regression analysis, statically significant risk factors of hyperuhcemia were gender, body mass index, blood lead concentration, and serum creatinine. Conclusion : Our results suggest that gender, body mass index, blood lead concentration, and serum creatinine might be the most important risk factors of hyperurcemia. Education program as well as routine check-up for serum uric acid was highly recommended for the effective prevention of hyperuricemia.

소음인(少陰人) 태음병(太陰病) 심하비증(心下?證)의 병증약리(病證藥理) 고찰

서영광,이지원,이준희,최원철,이의주,고병희,Seo, Young-Kwang,Lee, Ji-Won,Lee, Jun-Hee,Choi, Won-Cheol,Lee, Eui-Ju,Kho, Byung-Hee 사상체질의학회 2010 사상체질의학회지 Vol.22 No.3

1. Objective: Gastric Stuffiness (心下?證) in the Soeumin Taeeum symptomatology (少陰人 太陰病) in Sasang Constitutional Medicine shows an interesting evolutionary process. Originally found in the texts of Sanghanlun (傷寒論), the perspectives regarding the symptoms of Gastric Stuffiness were expanded by many medical scholars and Lee Jema. To comprehend the Jema's concept of Taeeum symptomatology and utilize his prescription clinically, it deserves to study on the Gastric Stuffiness in Donguisuseowon (東醫壽世保元). 2. Methods: Many texts including Sanghanlun (傷寒論) and Dongeuibogam (東醫寶鑑) were reviewed in order to track the evolutionary process concerning the symptoms of Gastric Stuffiness. Also, Lee Jema's works including the different versions of Donguisuseowon (東醫壽世保元) were reviewed to examine the development of Lee's concepts regarding the Gastric Stuffiness in the Soeumin Taeeum symptomatology. 3. Results and Conclusions: 1) Most of the previous concepts on Gastric Stuffiness were generally similar to those found in Sanghanlun, especially in that they addressed Yang deficiency of the middle triple energizeer as the central pathogenic process. 2) Lee Jema's views on the epigastric discomfort in the Soeumin Taeeum symptomatology evoloved over time and culminated in the finalized pathology and therapeutics appearing in his last work, the Shinchuk version of Donguisuseowon (東醫壽世保元). 3) 4 kinds of prescription which invented by Lee Jema to treat the Gastric Stuffiness in the Soeumin Taeeum symptomatology can be matched to the prescription of Sanghanlun respectively. However through evolutionary process of Jema's prescriptions, the indication of them expanded: not only to treat Gastric Stuffiness but to diverse symptoms of Soeumin.

휴대폰 카메라용 자동초점 구동기의 충격해석

이성민,김봉석,송준호,이수훈,이혜진,이문구,송준엽,이창우 한국공작기계학회 2008 한국공작기계학회 춘계학술대회논문집 Vol.2008 No.-

Recently, the robustness against daily impact is important according to portablization and downsizing of mobile electronics. Especially, almost parts of cellular phone should undergo drop test when they fall 1.5 m above ground. This test simulates the case when cellular phone slips through user's fingers while he is talking on the phone. This paper studies a drop test of auto focusing (AF) actuator for camera module in cellular phone. This component is composed of voice coil motor (VCM) as an actuator and leaf spring as a guide and suspension. The leaf spring's deformation is essential for the test because its permanent distortion disables the focusing, and then, a high quality photograph cannot be obtained. Up to now the drop test has been carried out after fabricating real AF actuator. We propose a dropt test model which simulates the drop test based on finite element analysis. This model makes us enable investigate the stress acting on the clamping and curved parts of leaf spring. Stress over Von Mises criterion lets the spring deformed permanently, and then AF actuator malfunctioned, It helps us to design and modify AF actuator without manufacturing the real product. And also, it saves the time and cost for the development of new products.

Effect of Impact Velocity on Interface Characteristics of HT‑9 Steel Joints Fabricated by Magnetic Pulse Welding

Jun‑Woo Song,Jin‑Ju Park,Gyoung‑Ja Lee,Min‑Ku Lee,Kyu‑Hyun Park,Soon‑Jik Hong,Jung Gu Lee 대한금속·재료학회 2020 METALS AND MATERIALS International Vol.26 No.3

The weldability window in magnetic pulse welding (MPW) is proposed for the end-closure joining of HT-9 steel tubularworkpieces. Based on both theoretical and experimental investigations, the impact velocity of the flyer tube at the momentof collision had a dominant effect on the bonding characteristics of the resultant joints and the optimal impact velocity wasestimated to be in the range of 265–290 m/s in this alloy system. Under the optimal process conditions, no leak was detectedduring helium leak testing. Moreover, the integrity of the joint interface was preserved until the tube section ruptured duringinternal pressure burst testing. For HT-9 steel, the ductile δ-ferrite phase produced by the localized interfacial melting duringMPW acted as an effective obstacle to crack propagation, thereby enhancing the fracture resistance of the joint interface. Allof the results demonstrated the high integrity and durability of the HT-9 steel tube assembly fabricated by the MPW process.

Insecticidal toxicities of carvacrol and thymol derived from Thymus vulgaris Lin. against Pochazia shantungensis Chou & Lu.

Jun?Hwan Park,Na-Hyun Lee,Sang-Guei Lee,Hoi-Seon Lee 한국농약과학회 2017 한국농약과학회 학술발표대회 논문집 Vol.2017 No.4

특성화고등학교 학생들의 과학 수업에서의 흥미에 관한 내러티브 연구: 전공 교과목과의 비교를 중심으로

장준규 ( Jun-kyu Chang ),이준기 ( Jun-ki Lee ) 전북대학교 과학교육연구소 2023 과학과 과학교육 논문지 Vol.48 No.1

이 연구는 특성화 고등학교에 입학한 학생 4명의 자발적 참여를 통해 이루어 졌다. 이 연구 대상자들이 어떠한 부분에서 과학에 대한 흥미를 경험했고, 무엇이 과학에 대한 흥미를 잃게 되었는지에 대해 연구 참여자들의 관점에서 확인하고 분석하고자 내러티브 탐구 방법을 통해 깊이 있는 면담을 진행했다. 이 연구의 목적은 특성화 고등학교 학생들이 과학에 흥미를 잃게 하는 다양한 요인들을 파악하는데 있다. 본 연구의 주요 결론을 제시하면 다음과 같다. 첫째, 연구 참여 학생들은 초 · 중학교시기에 연구 참여 학생들은 과학에 대한 흥미를 어느 정도 가지고 있었지만 특성화 고등학교에 입학 후 잃게 되었다. 둘째, 학생들이 과학교과에 흥미를 느낄 수 있게 해주는 환경이 조성되지 못했다. 결론을 종합하면 연구 참여 학생들은 과학교과에 대한 필요성을 인지하고 있음에도 불구하고, 과학교과에 대한 흥미를 느끼지 못해 수업에 집중할 수 없었다. 또한 연구 참여학생들의 과학교과는 일관적인 이론수업으로 진행되었고, 그 과정에서 학생들의 이해도는 고려되지 않았다. 결국 학생들은 과학교과에 대해 어려움을 느꼈고 흥미를 잃게 되었다. This study was conducted through the voluntary participation of four students who entered a vocational high school. The purpose of this research was to identify various factors that cause students at vocational high schools to lose interest in science, by examining and analyzing from the perspectives of the research participants which parts of science they experienced an interest in and what caused them to lose interest. In-depth interviews were conducted using narrative inquiry method. The main findings of this study are as follows. First, the participating students had some interest in science during their elementary and middle school years, but lost it after entering the vocational high school. Second, the environment that allows students to develop an interest in science was not provided. In conclusion, although the participating students were aware of the importance of science, they could not focus on class because they did not have an interest in science. In addition, their science classes consisted of consistent theoretical lessons, without considering the students' level of understanding. As a result, the students found science classes difficult and lost interest.