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Zhao Chenyue,Huang Qunxia,Qian Yuxin,Zhao Xuhui,Guo Shigang,Kan Yunchao,Li Dandan 한국응용곤충학회 2023 Journal of Asia-Pacific Entomology Vol.26 No.3
The cuticle supports the insect body and serves as a protective barrier against pathogens and pesticides. We found that the gene CPH19, encoding the hypothetical cuticular protein 19 (CPH19) of the silkworm Bombyx mori, was highly expressed in the cuticle during larval molt. Injection of CPH19 double-stranded RNA (dsRNA) into the hemolymph of 2-day-old third instar larvae (2dLa3) accelerated insect molting at the La3-La4 and La4-La5 transitions. Nevertheless, the larvae injected with CPH19 dsRNA had difficulty shedding their cuticle and suffered from molt defects, and their exuviae were larger and heavier than those of the control group. Morphological comparison of the exuviae between CPH19 dsRNA-injected and control larvae showed that the exocuticle-endocuticle layer was thicker in the former. The effect of CPH19 dsRNA injection on gene expression was further investigated by quantitative real-time polymerase-chain reaction (qRT-PCR). Surprisingly, the mRNA levels of CPH19 and other CP genes such as CPH18, CPH20, CPR69, CPR96, CPR97, CPR98 and CPR100, as well as chitinase genes such as chs-2 and chsA, were significantly increased in the CPH19 dsRNA-injected insects compared to the control. Bioinformatic predictions and luciferase assay analyses identified a proximal promoter near the transcription start site of CPH19 with strong transcriptional activity that may be involved in an RNAbased promoter targeting mechanism mediated by small activating RNAs (saRNAs). Treatment with the insec ticide decamethrin revealed that CPH19 dsRNA-injected larvae were more resistant to insecticides than control larvae, which could be exploited for the development of new pest control strategies.
Wang, Dandan,Zhao, Gong-Bo,Wang, Yuting,Percival, Will J,Ruggeri, Rossana,Zhu, Fangzhou,Tojeiro, Rita,Myers, Adam D,Chuang, Chia-Hsun,Baumgarten, Falk,Zhao, Cheng,Gil-Marí,n, Hé,ctor,Ross, Oxford University Press 2018 MONTHLY NOTICES- ROYAL ASTRONOMICAL SOCIETY Vol.477 No.2
<B>Abstract</B><P>We present a measurement of the anisotropic and isotropic Baryon Acoustic Oscillations (BAO) from the extended Baryon Oscillation Spectroscopic Survey Data Release 14 quasar sample with optimal redshift weights. Applying the redshift weights improves the constraint on the BAO dilation parameter α(zeff) by 17 per cent. We reconstruct the evolution history of the BAO distance indicators in the redshift range of 0.8 < z < 2.2. This paper is part of a set that analyses the eBOSS DR14 quasar sample.</P>
Dandan Song,Sohaib Shujaat,Ruiting Zhao,Yan Huang,Eman Shaheen,Jeroen Van Dessel,Kaan Orhan,Greetje Vande Velde,Ruxandra Coropciuc,Ruben Pauwels,Constantinus Politis,Reinhilde Jacobs 대한영상치의학회 2020 Imaging Science in Dentistry Vol.50 No.3
Purpose: This study was performed to introduce an in vivo hybrid multimodality technique involving the coregistration of micro-computed tomography (micro-CT) and high-resolution magnetic resonance imaging (HR-MRI) to concomitantly visualize and quantify mineralization and vascularization at follow-up in a rat model. Materials and Methods: Three adult female rats were randomly assigned as test subjects, with 1 rat serving as a control subject. For 20 weeks, the test rats received a weekly intravenous injection of 30 μg/kg zoledronic acid, and the control rat was administered a similar dose of normal saline. Bilateral extraction of the lower first and second molars was performed after 10 weeks. All rats were scanned once every 4 weeks with both micro-CT and HR-MRI. Micro-CT and HR-MRI images were registered and fused in the same 3-dimensional region to quantify blood flow velocity and trabecular bone thickness at T0 (baseline), T4 (4 weeks), T8 (8 weeks), T12 (12 weeks), T16 (16 weeks), and T20 (20 weeks). Histological assessment was the gold standard with which the findings were compared. Results: The histomorphometric images at T20 aligned with the HR-MRI findings, with both test and control rats demonstrating reduced trabecular bone vasculature and blood vessel density. The micro-CT findings were also consistent with the histomorphometric changes, which revealed that the test rats had thicker trabecular bone and smaller marrow spaces than the control rat. Conclusion: The combination of micro-CT and HR-MRI may be considered a powerful non-invasive novel technique for the longitudinal quantification of localized mineralization and vascularization
Dandan BIAN,Xiaoming ZHAO,Li CHEN,Jiwu TIAN,Qiuning LIU,Chunlin ZHOU,Boping Tang 한국곤충학회 2018 Entomological Research Vol.48 No.1
Eukaryotic initiation factor 5A (eIF‐5A) is a highly conserved protein found in all eukaryotic organisms that plays a key role in the regulation of many cellular processes including translation elongation, cell proliferation, programmed cell death, mRNA turnover and decay, and abiotic stress responses. In this study, the eIF‐5A gene from the Chinese oak silkworm Antheraea pernyi (Lepidoptera: Saturniidae) was characterized. The full‐length ApeIF‐5A cDNA of 1056 bp includes a 5′‐untranslated region (UTR) of 138 bp, a 3′‐UTR of 435 bp, and an open reading frame of 483 bp encoding a polypeptide of 160 amino acids. The deduced ApeIF‐5A protein shares 99 %, 82 %, and 72 % sequence identity with orthologs in Bombyx mori, Drosophila melanogaster and Homo sapiens, indicating high conservation during animal evolution. Real‐time quantitative reverse transcription PCR revealed expression in all four developmental stages and in all nine tissues tested, consistent with an important role in development. After challenge with lipopolysaccharide, the expression levels of ApeIF‐5A were markedly upregulated. Phylogenetic analysis of amino acid sequences revealed A. pernyi eIF‐5A was closely related to B. mori eIF‐5A, consistent with traditional classification and other molecular data. The results indicate the potential value of eIF‐5A in phylogenetic analysis.
Structural Studies of G Protein-Coupled Receptors
Dandan Zhang,Beili Wu,Qiang Zhao 한국분자세포생물학회 2015 Molecules and cells Vol.38 No.10
G protein-coupled receptors (GPCRs) constitute the largest and the most physiologically important membrane protein family that recognizes a variety of environmental stimuli, and are drug targets in the treatment of numerous diseases. Recent progress on GPCR structural studies shed light on molecular mechanisms of GPCR ligand recognition, activation and allosteric modulation, as well as structural basis of GPCR dimerization. In this review, we will discuss the structural features of GPCRs and structural insights of different aspects of GPCR biological functions.
Yan Zhao,Hongyu Liang,Dandan Wu,Junjia Bian,Yanping Hao,Guibao Zhang,Sanrong Liu,Huiliang Zhang,Lisong Dong 한국고분자학회 2015 폴리머 Vol.39 No.2
Poly(1,2-propylene glycol adipate) (PPA) was used as an environmentally friendly plasticizer in flexible poly(vinyl chloride) (PVC). Thermal, mechanical, and rheological properties of the PVC/PPA blends were characterized by differential scanning calorimetry, dynamic mechanical analysis, tensile test, scanning electron microscopy and small amplitude oscillatory shear rheometry. The results showed that PPA lowered the glass transition temperature of PVC. The introduction of PPA could decrease tensile strength and Young’s modulus of the PVC/PPA blends; however, elongationat-break was dramatically increased due to the plastic deformation. The plasticization effect of PPA was also manifested by the decrease of dynamic storage modulus and viscosity in the melt state of the blends. The results indicated that PPA had a good plasticizing effect on PVC.