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- 저자 : Chunlin ZHOU (검색결과 4 건)
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Cloning and expression of a Chk1 gene in Daphnia pulex during different modes of reproduction
Xiaoge Guo,Shanliang Xu,Xuemei Yan,Wei Zhou,Xinyu Dai,Xiu Zou,Chunlin Wang,Danli Wang,Yunlong Zhao 한국유전학회 2015 Genes & Genomics Vol.37 No.9
The full-length cDNA of a Chk1 gene (DpChk1) was cloned from Daphnia pulex using RACE method. This cDNA is 1767 bp in length, consisting of a 1497 bp open reading frame encoding 498 amino acid protein. The deduced DpChk1 protein sequence contains phosphorylation sites, three conserved Ser-Gln and Thr- Gln motifs and shares 51–55 % similarity with A. pisum, M. rotundata, D. melanogaster, A. echinatior, B. terrestris, R. pulchellus and M. occidentalis, respectively. Gene expression analysis showed that Chk1 is differentially expressed in D. pulex during different reproductive modes. DpChk1 expression in sexually-reproducing females and males is significantly higher than in parthenogenetic Daphnia. Whole-mount in situ hybridization revealed that DpChk1 was expressed during two kinds of reproductive modes. In parthenogenetic females, hybridization signals were found in the antennae and thoracic limb, whereas expression levels in the corresponding sites of sexuallyreproducing females were relatively strong, DpChk1 could not be detected in the gonads of ephippial and parthenogenetic females. Taken together, these different reproductive stages and sex specific expression patterns are regulated temporally and spatially. We speculate that DpChk1 may be involved in conversion between different stages of reproduction and in sexual differentiation in D. pulex.
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Inhomogeneous Fatigue Characteristics of Welded Ti–6Al–4V Thick Plate by the Electron Beam Method
Haiyan Wang,Caiyou Zeng,Caiyou Zeng,Yupeng Zhang,Yu Zhou,Chunlin Dong 대한금속·재료학회 2021 METALS AND MATERIALS International Vol.27 No.11
To recognize the specific safety problem brought by an increasing thickness, the layered microstructures, fatigue propertiesand the fractographies of a 100 mm-thick Ti–6Al–4V joint via electron beam welding were investigated. The results showthat the seam zone has textural and mechanical heterogeneities in the layered thickness regions. The mid layer has the lowestfatigue life and different fracture-position tendency (more broken in seam zone) due to the hybrid influences of the grain sizeand the phase fraction of α′ and β phases. The fatigue cracks initiate on the surfaces of fatigue specimens and grow in thetransgranular fracture mode. The fatigue deformation mode is adapted to the planar-slip mechanism with different degreesof dislocation blocking in distinct layers, which results in a “mid-layer favorite” crack initiation trend. Moreover, the midlayer has the highest crack propagation rate either.
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Dandan BIAN,Xiaoming ZHAO,Li CHEN,Jiwu TIAN,Qiuning LIU,Chunlin ZHOU,Boping Tang 한국곤충학회 2018 Entomological Research Vol.48 No.1
Eukaryotic initiation factor 5A (eIF‐5A) is a highly conserved protein found in all eukaryotic organisms that plays a key role in the regulation of many cellular processes including translation elongation, cell proliferation, programmed cell death, mRNA turnover and decay, and abiotic stress responses. In this study, the eIF‐5A gene from the Chinese oak silkworm Antheraea pernyi (Lepidoptera: Saturniidae) was characterized. The full‐length ApeIF‐5A cDNA of 1056 bp includes a 5′‐untranslated region (UTR) of 138 bp, a 3′‐UTR of 435 bp, and an open reading frame of 483 bp encoding a polypeptide of 160 amino acids. The deduced ApeIF‐5A protein shares 99 %, 82 %, and 72 % sequence identity with orthologs in Bombyx mori, Drosophila melanogaster and Homo sapiens, indicating high conservation during animal evolution. Real‐time quantitative reverse transcription PCR revealed expression in all four developmental stages and in all nine tissues tested, consistent with an important role in development. After challenge with lipopolysaccharide, the expression levels of ApeIF‐5A were markedly upregulated. Phylogenetic analysis of amino acid sequences revealed A. pernyi eIF‐5A was closely related to B. mori eIF‐5A, consistent with traditional classification and other molecular data. The results indicate the potential value of eIF‐5A in phylogenetic analysis.
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Jing Zhang,Jing Wang,Xiong Zhang,Chunping Zhao,Sixuan Zhou,Chunlin Du,Ya Tan,Yu Zhang,Kaizhi Shi 대한수의학회 2022 Journal of Veterinary Science Vol.23 No.1
Background: Co-infections of the porcine reproductive and respiratory syndrome virus (PRRSV) and the Haemophilus parasuis (HPS) are severe in Chinese pigs, but the immune response genes against co-infected with 2 pathogens in the lungs have not been reported. Objectives: To understand the effect of PRRSV and/or HPS infection on the genes expression associated with lung immune function. Methods: The expression of the immune-related genes was analyzed using RNA-sequencing and bioinformatics. Differentially expressed genes (DEGs) were detected and identified by quantitative real-time polymerase chain reaction (qRT-PCR), immunohistochemistry (IHC) and western blotting assays. Results: All experimental pigs showed clinical symptoms and lung lesions. RNA-seq analysis showed that 922 DEGs in co-challenged pigs were more than in the HPS group (709 DEGs) and the PRRSV group (676 DEGs). Eleven DEGs validated by qRT-PCR were consistent with the RNA sequencing results. Eleven common Kyoto Encyclopedia of Genes and Genomes pathways related to infection and immune were found in single-infected and co-challenged pigs, including autophagy, cytokine-cytokine receptor interaction, and antigen processing and presentation, involving different DEGs. A model of immune response to infection with PRRSV and HPS was predicted among the DEGs in the co-challenged pigs. Dual oxidase 1 (DUOX1) and interleukin-21 (IL21) were detected by IHC and western blot and showed significant differences between the co-challenged pigs and the controls. Conclusions: These findings elucidated the transcriptome changes in the lungs after PRRSV and/or HPS infections, providing ideas for further study to inhibit ROS production and promote pulmonary fibrosis caused by co-challenging with PRRSV and HPS.
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