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RISS 인기검색어
- 검색키워드
- 저자 : Qian Yuxin (검색결과 4 건)
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Zhao Chenyue,Huang Qunxia,Qian Yuxin,Zhao Xuhui,Guo Shigang,Kan Yunchao,Li Dandan 한국응용곤충학회 2023 Journal of Asia-Pacific Entomology Vol.26 No.3
The cuticle supports the insect body and serves as a protective barrier against pathogens and pesticides. We found that the gene CPH19, encoding the hypothetical cuticular protein 19 (CPH19) of the silkworm Bombyx mori, was highly expressed in the cuticle during larval molt. Injection of CPH19 double-stranded RNA (dsRNA) into the hemolymph of 2-day-old third instar larvae (2dLa3) accelerated insect molting at the La3-La4 and La4-La5 transitions. Nevertheless, the larvae injected with CPH19 dsRNA had difficulty shedding their cuticle and suffered from molt defects, and their exuviae were larger and heavier than those of the control group. Morphological comparison of the exuviae between CPH19 dsRNA-injected and control larvae showed that the exocuticle-endocuticle layer was thicker in the former. The effect of CPH19 dsRNA injection on gene expression was further investigated by quantitative real-time polymerase-chain reaction (qRT-PCR). Surprisingly, the mRNA levels of CPH19 and other CP genes such as CPH18, CPH20, CPR69, CPR96, CPR97, CPR98 and CPR100, as well as chitinase genes such as chs-2 and chsA, were significantly increased in the CPH19 dsRNA-injected insects compared to the control. Bioinformatic predictions and luciferase assay analyses identified a proximal promoter near the transcription start site of CPH19 with strong transcriptional activity that may be involved in an RNAbased promoter targeting mechanism mediated by small activating RNAs (saRNAs). Treatment with the insec ticide decamethrin revealed that CPH19 dsRNA-injected larvae were more resistant to insecticides than control larvae, which could be exploited for the development of new pest control strategies.
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m6A writer gene fl(2)d regulates the metamorphosis of silkworm Bombyx mori
Huang Qunxia,Huo Chunyue,Qian Yuxin,Zhao Chenyue,Cai Zhiyan,Zhang Kun,Zhao Xuhui,Guo Shigang,Kan Yunchao,Li Dandan 한국응용곤충학회 2023 Journal of Asia-Pacific Entomology Vol.26 No.4
The N6-methyladenosine (m6A) is one of the most abundant posttranscriptional modifications on RNAs, which is mediated by its effector proteins—writers, readers, and erasers. Female-Lethal(2)D (Fl(2)d) is one of the key writers to stabilize the interaction between another two writers Methyltransferase-like 3 (METTL3) and Methyltransferase-like 14 (METTL14). Here with dsRNA transfection, fl(2)d was knocked down in the BmN4 cell lines of silkworm, the expression of 61 genes were significantly increased, and 86 genes were significantly decreased after fl(2)d repression, which encoded proteins related to catalytic, hydrolase activity or in metabolism of nucleic acid. To elucidate the functional role of fl(2)d during silkworm development, dsRNA of which was injected into the hemolymph of the 2-d-old fourth instar larvae, the mounting process of silkworm was delayed, the expression level of genes in metabolism, RNA processing and ecdysone signaling pathway decreased significantly after fl(2)d knockdown, such as genes encoded Ubiquitin protein ligase E3C, the nuclear pore complex protein Nup98-Nup96, and the m6A reader YTHDF3, as well as Broad Complex isoform 2 (BRC-Z2), Fushi Tarazu Factor-1 (FTZ-F1), lactate dehydrogenase (LDH) and the ecdysone-induced protein 74EF isoform A (E74A). Total m6A level of RNAs also decreased after fl(2)d repression. These indicated that decreased level of fl (2)d and YTHDF3 decay the m6A level and translation of their target genes, which might lead to the retardation of metamorphosis, and resulted in the delayed mounting of silkworm.
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The Physiological Occlusion of the Central Canal May Be a Prerequisite for Syringomyelia Formation
Chuan Jiang,Xinyu Wang,Chunli Lu,Qian Li,Longbing Ma,Wei Li,Shengyu Cui,Kang Li,Xiang Wang,Yuxin Feng,Fengzeng Jian 대한척추신경외과학회 2023 Neurospine Vol.20 No.4
Objective: Syringomyelia is a common central nervous system disease characterized by the dilation of the central canal (CC). Regarding the pathogenesis of syringomyelia, cerebrospinal fluid (CSF) circulation obstruction in the subarachnoid space (SAS) of the spinal cord has been widely accepted. However, clinical and animal studies on obstructing the CSF in SAS failed to form syringomyelia, challenging the theory of SAS obstruction. The precise pathogenesis remains unknown. Methods: We utilized an extradural compression rat model to investigate the pathogenesis underlying syringomyelia. Magnetic resonance imaging enabled detection of syringomyelia formation. To assess CSF flow within the SAS, Evans blue was infused into the cisterna magna. Histological analysis allowed morphological examination of the CC. Furthermore, CSF flow through the CC was traced using Ovalbumin Alexa-Flour 647 conjugate (OAF-647). Scanning electron microscopy (SEM) enabled visualization of ependymal cilia. Results: The findings showed that the dura mater below the compression segment exhibited lighter coloration relative to the region above the compression, indicative of partial obstruction within the SAS. However, the degree of SAS occlusion did not significantly differ between syringomyelia (SM-Y group) and those without (SM-N group). Intriguingly, hematoxylin and eosin staining and CSF tracing revealed occlusion of the CC accompanied by reduced CSF flow in the SM-Y group compared to SM-N and control groups. SEM images uncovered impairment of ependymal cilia inside the syringomyelia. Conclusion: CC occlusion may represent a physiological prerequisite for syringomyelia formation, while SAS obstruction serves to initiate disease onset. The impairment of ependymal cilia appears to facilitate progression of syringomyelia.
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Sperm maturation screening and the effect of ecdysone on sperm development of silkworm Bombyx mori
Cheng Hao,Wang Yanyan,Chang Meiling,Zhang Kun,Cai Zhiyan,Qian Yuxin,Li Dandan 한국응용곤충학회 2022 Journal of Asia-Pacific Entomology Vol.25 No.2
There are two sperm morphs of silkworm, the nucleated spermatozoa (eupyrene) and anucleated spermatozoa (apyrene). Eupyrene sperm cannot complete fertilization successfully without the apyrene sperm. Here a modified rapid and efficient method for sperm identification was developed, after 10 s of fixation in para formaldehyde and 30 s of 4 ′ 6-diamidino-2-phenylindole (DAPI) or propidium Iodide (PI) staining, the sperm bundles can be detected easily using a fluorescence microscope. Sperm maturation process of silkworm from the fifth instar larvae to the adult was described with the above method, the precise time of earliest elongate apyrene bundles was detected on day 2 of pre-pupation, with a ratio of 5% in total sperm bundles, after which the percentage of apyrene sperm bundles increased rapidly and attained a relatively stable ratio of 75% at the end of pupation and nearly 80% after eclosion. Delayed mating leads to apyrene sperm accumulation and damaged fertilization. Previous study showed that ecdysone can increase the frequency of apyrene sperm bundles in vitro. Here 20-hydroxyecdysone (20E) was injected into hemolymph of the 2-d-old fifth instar larvae, the worms entered into mounting period after three days injection, but no apyrene sperm bundles were induced unless day 2 of pre-pupation. Interestingly, maturation of eupyrene sperm bundles were accelerated, and the ratio of eupyrene sperm bundles increased and exhibited a dose-dependent effect after 20E injection, which indicated that the development of eupyrene sperm can be accelerated by ecdysone before pupation of silkworm in vivo. These results will provide new clues for lepidopteran pest control.
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