Heme oxygenase-1 induced by desoxo-narchinol-A attenuated the severity of acute pancreatitis via blockade of neutrophil infiltration

Bae, Gi-Sang,Kim, Dong-Goo,Jo, Il-Joo,Choi, Sun-Bok,Kim, Myoung-Jin,Shin, Joon Yeon,Kim, Dong-Uk,Song, Ho-Joon,Joo, Myungsoo,Park, Sung-Joo ELSEVIER 2019 INTERNATIONAL IMMUNOPHARMACOLOGY Vol.69 No.-

<P><B>Abstract</B></P> <P>Heme oxygenase-1 (HO-1) has an anti-inflammatory action in acute pancreatitis (AP). However, its mechanism of action and natural compounds/drugs to induce HO-1 in pancreas are not well understood. In this study, we investigated the regulatory mechanisms of HO-1 during AP using desoxo-narchinol-A (DN), the natural compound inducing HO-1 in the pancreas. Female C57/BL6 Mice were intraperitoneally injected with supramaximal concentrations of cerulein (50 μg/kg) hourly for 6 h to induce AP. DMSO or DN was administered intraperitoneally, then mice were sacrificed 6 h after the final cerulein injection. Administration of DN increased pancreatic HO-1 expression through activation of activating protein-1, mediated by mitogen-activated protein kinases. Furthermore, DN treatment reduced the pancreatic weight-to-body weight ratio as well as production of digestive enzymes and pro-inflammatory cytokines. Inhibition of HO-1 by tin protoporphyrin IX abolished the protective effects of DN on pancreatic damage. Additionally, DN treatment inhibited neutrophil infiltration into the pancreas via regulation of chemokine (C-X-C motif) ligand 2 (CXCL2) by HO-1. Our results suggest that DN is an effective inducer of HO-1 in the pancreas, and that HO-1 regulates neutrophil infiltration in AP via CXCL2 inhibition.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Desoxo-narchinol-A (DN) is a natural compound of HO-1 inducer in pancreas. </LI> <LI> Mechanism of DN-induced HO-1 is mediated by MAPK/Activator Protein-1/HO-1 signaling. </LI> <LI> DN-induced HO-1 blocks neutrophil infiltration into pancreas via inhibition of CXCL2. </LI> <LI> DN inhibits cerulein-induced acute pancreatitis (AP) and AP-associated lung injury. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

도시근린공원 방범설계 (CPTED)의 효과성 검증

홍소연(Hong, So Yeon),권순호(Kwon, Soom Ho),박세희(Park, Se Hee),김은기(Kim, Eun Kee) 한국공안행정학회 2020 한국공안행정학회보 Vol.29 No.1

이 연구는 도시근린공원에서의 범죄예방환경설계 효과성 검증을 목적으로 실시되었다. 연구목적 달성을 위해 선행연구에서 활용된 범죄예방환경설계 평가항목을 활용하여 대상 공원 두 곳의 범죄예방환경설계 수준을 객관적으로 평가하였고, 설계 수준이 우수한 공원과 낮은 공원 방문객들을 대상으로 각각 범죄피해두려움을 측정하였다. 분석결과 설계 수준이 우수한 공원보다 설계 수준이 낮은 공원에서 방문객들의 범죄피해두려움이 더 높게 나타남을 확인 할 수 있었으며, 두 공원에서 느끼는 범죄피해두려움의 평균차이는 통계적으로 유의한 차이로 확인되었다. 따라서 도시근린공원에서의 범죄예방환경설계는 이용객들의 범죄피해 두려움을 낮추는 효과가 있다고 할 수 있다. 그러나 이 연구는 범죄피해두려움에 영향을 미치는 다른 요인들을 통제하지 못하였다는 점, 조사대상 공원의 범죄예방환경설계 수준에 대한 평가의 정교성이 다소 낮다는 점 등의 한계가 존재한다. 그럼에도 불구하고 ‘CPTED 인식’과 범죄피해두려움의 관계를 주로 검토하여 효과성을 검증하고자 한 기존 선행연구와는 달리 객관적 평가척도를 활용하여 설계수준 차이에 따른 범죄피해 두려움의 차이를 검증함으로써 CPTED의 효과성을 검증하였다는 점에서 의의가 있는 연구라 하겠다. This study was conducted to verify the effectiveness of crime prevention environment design in urban neighborhood parks. To achieve the objectives of the research, the crime prevention environmental design evaluation items used in the previous studies were objectively evaluated, and the crime prevention environment design levels of two target parks were objectively evaluated. Fear of crime was measured. As a result, visitors were found to have higher fears of crime victimization in parks with lower design level than those with better design level, and the average difference between crime fears felt between two parks was statistically significant. Therefore, the design of crime prevention environment in urban neighborhood parks can be said to reduce the fear of crime victims. However, this study has limitations such as the lack of control of other factors affecting the fear of crime, and the low level of elaboration of the assessment of crime prevention environment design of the park under investigation. Nevertheless, unlike previous studies that mainly examined the relationship between CPTED awareness and fear of crime, CPTED by verifying the difference of crime damage fear according to the design level by using objective evaluation scale. It is a meaningful study in that it verified the effectiveness of.

Protein microarray를 이용한 Apin-단백질의 상호작용에 관한 연구

박주철,박선화,김흥중,박종태,윤성호,김지웅,이태연,손호현 대한치과보존학회 2007 Restorative Dentistry & Endodontics Vol.32 No.5

무등산국립공원 군부대 주둔지역 복원 종합계획^a

최윤호 ( Yeon-ho Choi ),오구균 ( Koo Kyun Oh ),나경태 ( Kyong Tae Na ),이영주 ( Young Ju Lee ),조우 ( Woo Cho ),오현경 ( Hyun Kyung Oh ),박석곤 ( Seok Gon Park ),김영선 ( Young Sun Kim ),최옥곤 ( Ok Gon Choi ),윤주옥 ( Ju Og Yun 한국환경생태학회 2017 한국환경생태학회 학술대회지 Vol.2017 No.1

Isolation of Megastigmane Sesquiterpenes from the Silkworm (Bombyx mori L.) Droppings and Their Promotion Activity on HO-1 and SIRT1

Ji-Hae Park,Do-Gyeong Lee,Seung-Woo Yeon,Hyuk-Sang Kwon,Jong-Hee Ko,Dong-Jin Shin,Han-Sol Park,Yong-Soon Kim,Myun-Ho Bang,NAM-IN BAEK 대한약학회 2011 Archives of Pharmacal Research Vol.34 No.4

The silkworm (Bombyx mori L.) droppings were extracted with 80% aqueous MeOH, and the concentrated extract was partitioned in succession with EtOAc, n-BuOH, and H_2O. From the EtOAc fraction, five megastigmane sesquiterpenes were isolated through repeated silica gel and ODS column chromatography. According to the results of spectroscopic data, such as NMR, MS, and IR, the chemical structures of the isolated compounds were determined as (3S,5R,8R)-3,5-dihydroxymegastigma-6,7-dien-9-one (1), (S)-dehydrovomifoliol (2), (6R,7E,9R)-9-hydroxy-4,7-megastigmadien-3-one (3), (3S,5R,6S,7E)-3,5,6-trihydroxy-7-megastigmen-9-one (4), (6R,9R)-9-hydroxy-4-megastigmen-3-one (5). Compounds 2 through 5 were isolated for the first time from silkworm droppings. GC/MS analysis indicated silkworm powder contained compound 3, and mulberry leaves contained compound 4. Compounds 1 and 5 increased the expression of heme oxygenase-1 and SIRT1 in HepG2 and HEK239 cells, respectively. Heme oxygenase-1 is considered to be an antioxidant enzyme that catabolizes heme to carbon monoxide, free iron and biliverdin, while SIRT1 is the mammalian homologue of the yeast silent information regulator (Sir)-2, which are involved in the suppression of inflammatory mediators or factors that may be used to improve atopy-related symptoms.

The Antioxidant Effect of the Rhaponticum uniflorum Extracts on the Oxidative Stress in H₂O₂ induced Human Keratinocytes

So Hee Ahn,Won Yeoung Choi,Ji Won Jeong,Geon Woo Park,Su Yeon Kang,Eun Jeong Kim,Su Rim Sin,Eun Seo Jang,Gye Won Lee,Young Ho Cho 한국식품영양과학회 2021 한국식품영양과학회 학술대회발표집 Vol.2021 No.10

In this study, To investigate the antioxidant activities of Rhaponticum uniflorum extracts (RUE), we assessed scavenging activities on DPPH, ABTS radicals, and reducing power. The DPPH and ABTS radical scavenging activities (SC50) of RUE were 96.97±1.19 μg/mL and 31.26±0.13 μ g/mL, respectively, and the reducing power significantly increased dose-dependent manner. To study the antioxidant effecters of RUE in H₂O₂ induced human keratinocytes, expression of Nuclear factor erythroid-2-related factor 2 (Nrf2) and heme oxygenase-1 (HO-1) were determined by Western blot and qRT-PCR. RUE induced the expression of HO-1, a critical target enzyme of Nrf2, in a concentration-dependent manner. The expression of HO- 1 mRNA was also induced in H₂O₂-treated HaCaT cells but no significant difference in the level of HO-1 mRNA was observed. Taken together, these results suggest that RUE may be useful as a potential ingredient for anti-aging products.

ImmunoDot법에 의한 전신성 류마티스 질환의 자가항체 검사

박성환,김선준,김성일,민준기,이상헌,홍연식,조철수,김호연 최신의학사 1997 最新醫學 Vol.40 No.7

태음인 체질의 비만아동에 대한 운동처방

박기호,정연희 仁川敎育大學校 科學敎育硏究所 2003 과학교육논총 Vol.15 No.-

본 연구는 비만해소 방안과 효과의 검토 및 자료를 제시하기 위하여, 비만도 20% 이상 초등학교 5·6학년 남학생 중 체질진단결과 태음인체질의 10명을 대상으로 10주 동안 주4회, 50∼70% HRmax의 강도로 운동처방프로그램을 실시하여 운동처방 전·후의 신장, 체중, 혈압, 심박수, 체지방량의 신체적 변화와 특수 인성검사A의 심리적 변화를 측정하였으며, 자료분석은 SPSS통계프로그램의 비모수 검정의 Wilcoxon 부호순위 검정을 이용하였다. 신체적 변화에서는 신장은 근사 유의확률이 P < .005, 혈압은 수축기와 이완기에서 P < .011과 P < .026, 체지방량은 P < .028로 유의한 것으로 나타났으나, 신장은 운동처방에 의한 영향이라고 볼 수 없으므로 유의한 차이가 없는 것이 타당하다. 체중과 심박수에서는 유의한 차이가 나타나지 않았다. 심리적 변화에서는 타당도 척도(Validity Scales), 일반 성격척도(General Personality Scales), 특수적응척도(Special Adaptation Scales)에서는 유의한 차이가 나타나지 않았다. 아동비만을 사상의학의 바탕인 체질의학에서 체질적 특징과 비만에 대한 신체적·심리적 특징을 파악하여 체질에 맞는 10주간의 짧은 기간이지만 규칙적으로 꾸준히 운동을 실시한다면 비만을 억제하거나 치료할 수 있고, 나아가 성인비만으로 이환될 가능성을 예방할 수 있을 것이라 생각된다. The purpose of this study was to investigate the effective anti-obesity measures and its effects. The research was conducted to ten boys(the 5th and 6th graders in elementary school) whose obesity degree is over 20 percent. The subjects with Taeeum-In constitution are exposed to exercise programs with 50 to 70 HRmax for 10 weeks. The exercise prescription program was implemented 4 times a week. The physical and psychological changes was measured by the pre & post-program. The analysis of data was relied on Wilcoxon Signed Ranks Test among nonparametric tests(significant level P<.05). The results of this research are as follows: 1) in respect of the physical changes, the blood pressure is significant(P<.011 and P<.026) in the contraction period and the relaxation period respectively. The amount of body fat is significant(P<.028). But heights is not significant(in spite of P<.05) because it was not influenced by the exercise prescription. Weights and heart beats are also not significant; 2) in respect of the psychological changes, the desirability of validity scales and General personality scales and special adaptation scales are not significant. This study suggests that regular exercise in accordance with human constitution may treat obesity and prevent transferability for adult obesity.

환자 만족도 조사를 통한 QI활동의 효과 평가 : 일개 병원을 중심으로

박연옥,김미숙,고은정,김연정,홍창호 한국의료QA학회 1998 한국의료질향상학회지 Vol.5 No.2

Background : Evaluation of patient's satisfaction is one of the most important aspects of quality improvement. If the patient highly satisfies with the medical service provided in the hospital, he/she will be likely to visit the same hospital again. Patient's satisfaction of a particular hospital is directly correlated with hospital profits as well as reputation in the community. For this reason, various kinds of survey measuring satisfaction level have been performed and many kind of QI activities for enhancing the profits as well as reputation in the community. For this reason, various kinds of survey measuring satisfaction level have been performed and many kind of QI activities for enhancing the patient's satisfaction. This study is to find the effect of hospital QI activities on the patient's satisfaction level. Methods : After questionnares were developed, survey of measuring satisfaction level was performed in August. 1998. On the basis of survey results. Ql activities were carried out to attain the target point of 4.0 and subsequent survey was done in November. 1998. Results : With three main principles of "problem solving approach with kindness". "helping patient to participate in medical procedure with sufficient information", and "putting employees into practice of attitude with human respect", the average level of satisfaction was enhanced from 3.45 to 3.55 level. Also kindness level of employees was increased from 3.71 to 3.82. Level of dissatisfaction about insufficient explanation and unkind attitude was dropped from 69% to 48% and from 82% to 46% respectively. Conclusion : With the result of this study overall satisfaction level was enhanced. In order to keep these advantages a operation of Quality Improvement Task Force Team in each subject will be required.

건강한 성인에서 발병한 칸디다와 연관된 위궤양 1례

박지훈,이상혁,김희,이재호,박성재,지삼룡,양성연,박은택,이연재,설상영,정정명,강미선 白中央醫療院 2005 仁濟醫學 Vol.26 No.1

Fungus such as Candida albicans is a normal flora that is frequently found in the oral cavity, gastrointestinal tract, genitourinary tract, vaginal mucosa in a normal person. However, candida can cause opportunistic infection in an immune compromised host. Candidiasis has broad spectrum of disease from mucocutaneous infection to invasive or disseminated infection. But, it is rarely reported that candida is associaed with gastrointestinal tract disease in a healthy adult. The case of gastric ulcer associated with candida particularly in a health adult is reported with relevant literature.