miR-34a Inhibitor May Effectively Protect against Sevoflurane-Induced Hippocampal Apoptosis through the Wnt/β-Catenin Pathway by Targeting Wnt1

Xiaoling Zhao,Yue Sun,Yongbo Ding,Jun Zhang,Kezhong Li 연세대학교의과대학 2018 Yonsei medical journal Vol.59 No.10

Purpose: Research has shown that sevoflurane-induced toxicity causes neurodegeneration in the developing brain. miR-34a has been found to negatively regulate ketamine-induced hippocampal apoptosis and memory impairment. However, the role of miR-34a in sevoflurane-induced hippocampal neurodegeneration remains largely unclear. Materials and Methods: C57/BL6 mice (7-day-old) inhaled 2.3% sevoflurane for 2 h/day over 3 consecutive days. miR-34a expressionwas reduced through intracerebroventricular injection with miR-34a interference lentivirus vector (LV-anti-miR-34a) into mouse hippocampus after anesthesia on the first day of exposure. Hippocampal apoptosis was detected by TUNEL assay and flow cytometry analysis. Spatial memory ability was evaluated by the Morris water maze test. The interaction between miR-34a and Wnt1 was confirmed by luciferase reporter assay, RNA immunoprecipitation, Western blot, and immunofluorescence staining. The effects of miR-34a on protein levels of B-cell lymphoma 2 (Bcl-2), bcl-2-like protein 4 (Bax), and Wnt/β-catenin pathway-related proteins were evaluated using Western blot analysis. Results: Sevoflurane upregulated hippocampal miR-34a, and miR-34a inhibitor attenuated sevoflurane-induced hippocampal apoptosis and memory impairment. miR-34a negatively regulated Wnt1 expression by targeting miR-34a in hippocampal neurons. Moreover, forced expression of Wnt1 markedly undermined miR-34a-mediated enhancement of sevoflurane-induced apoptosis of hippocampal neurons, while Wnt1 silencing greatly restored anti-miR-34a-mediated repression of sevoflurane-inducedapoptosis of hippocampal neurons. Increased expression of miR-34a inhibited the Wnt/β-catenin pathway in hippocampalneurons exposed to sevoflurane, while anti-miR-34a exerted the opposite effects. Conclusion: miR-34a inhibitor may effectively protect against sevoflurane-induced hippocampal apoptosis via activation of the Wnt/β-catenin pathway by targeting Wnt1.

Dually flat and projectively flat Finsler warped product structures

Xiaoling Zhang,Xuesong Zhang,Lili Zhao 대한수학회 2022 대한수학회보 Vol.59 No.6

In this paper, we study the Finsler warped product metric which is dually flat or projectively flat. The local structures of these metrics are completely determined. Some examples are presented.

Histone demethylase KDM4A plays an oncogenic role in nasopharyngeal carcinoma by promoting cell migration and invasion

Zhao Jingyi,Li Bingyan,Ren Yongxia,Liang Tiansong,Wang Juan,Zhai Suna,Zhang Xiqian,Zhou Pengcheng,Zhang Xiangxian,Pan Yuanyuan,Gao Fangfang,Zhang Sulan,Li Liming,Yang Yongqiang,Deng Xiaoyu,Li Xiaole,C 생화학분자생물학회 2021 Experimental and molecular medicine Vol.53 No.-

Compelling evidence has indicated the vital role of lysine-specific demethylase 4 A (KDM4A), hypoxia-inducible factor-1α (HIF1α) and the mechanistic target of rapamycin (mTOR) signaling pathway in nasopharyngeal carcinoma (NPC). Therefore, we aimed to investigate whether KDM4A affects NPC progression by regulating the HIF1α/DDIT4/mTOR signaling pathway. First, NPC and adjacent tissue samples were collected, and KDM4A protein expression was examined by immunohistochemistry. Then, the interactions among KDM4A, HIF1α and DDIT4 were assessed. Gain- and loss-of-function approaches were used to alter KDM4A, HIF1α and DDIT4 expression in NPC cells. The mechanism of KDM4A in NPC was evaluated both in vivo and in vitro via RT-qPCR, Western blot analysis, MTT assay, Transwell assay, flow cytometry and tumor formation experiments. KDM4A, HIF1α, and DDIT4 were highly expressed in NPC tissues and cells. Mechanistically, KDM4A inhibited the enrichment of histone H3 lysine 9 trimethylation (H3K9me3) in the HIF1α promoter region and thus inhibited the methylation of HIF1α to promote HIF1α expression, thus upregulating DDIT4 and activating the mTOR signaling pathway. Overexpression of KDM4A, HIF1α, or DDIT4 or activation of the mTOR signaling pathway promoted SUNE1 cell proliferation, migration, and invasion but inhibited apoptosis. KDM4A silencing blocked the mTOR signaling pathway by inhibiting the HIF1α/DDIT4 axis to inhibit the growth of SUNE1 cells in vivo. Collectively, KDM4A silencing could inhibit NPC progression by blocking the activation of the HIF1α/DDIT4/mTOR signaling pathway by increasing H3K9me3, highlighting a promising therapeutic target for NPC.

The effects of main anoxic section oxidation-reduction potential on the metabolism of PHA and TP in continuous-flow single-sludge treatment system

Xiaoling Wang,Hai Lu,Tiehong Song,Ke Zhao 한국화학공학회 2019 Korean Journal of Chemical Engineering Vol.36 No.3

The experimental results and material balance analysis in this paper revealed the regularity of poly-hydroxy alkanoates (PHA) and total phosphorus (TP) metabolism in a continuous-flow single-sludge wastewater treatment system under different main anoxic section oxidation-reduction potential (ORPan) conditions. We also evaluated the effectiveness of the operation control parameters of ORPan as the continuous-flow single-sludge sewage treatment system from the aspect of the reaction mechanism. Using a programmable logic controller (PLC) automatic control system to take the circulating flow in nitrification as the controlled variable based on the feedback control structure, an experimental study was carried out under the condition of ORPan setting value of 143mV, 123mV, 105mV, 95mV, 72 mV and 57mV, respectively, with other operational design parameters remaining unchanged. Influent water quality of chemical oxygen demand/total nitrogen (COD/TN) was 5.0±0.6. The results showed that when ORPan was set at 95mV, the maximum values of PHA synthesis and storage rate, PHA degradation rate, phosphorus release rate and phosphorus absorption rate in anaerobic and pre-anoxic segments were 82.34, 7.90, 47.31, 14.27, 1.50 and 8.52mg/ (L·h), respectively. According to the metabolic mechanism of PHA and TP, ORPan was further proved to be the operation control parameter of the continuous-flow single-sludge sewage treatment system, and when the COD/TN value was 5.0±0.6, the optimal setting value was 95mV.

Phase II Trial of Epigallocatechin-3-Gallate in Acute Radiation-Induced Esophagitis for Esophagus Cancer

Xiaoling Li,Ligang Xing,Yujun Zhang,Peng Xie,Wanqi Zhu,Xiangjiao Meng,Yinxia Wang,Lingling Kong,Hanxi Zhao,Jinming Yu 한국식품영양과학회 2020 Journal of medicinal food Vol.23 No.1

Acute radiation-induced esophagitis (ARIE) is among the most serious form of toxicities associated with definitive radiotherapy or chemoradiotherapy used for treatment of patients with esophageal cancer. Our preliminary phase I and II trials of lung cancer patients who received radiotherapy indicated epigallocatechin-3-gallate (EGCG) as a promising therapeutic option against ARIE. Therefore, we conducted a prospective phase II study to validate the efficacy and safety of EGCG in the treatment of ARIE. The patients who received chemoradiotherapy or definitive radiotherapy for treatment of esophageal cancer in the Shandong Cancer Hospital and Institute in China were enrolled for the present study. EGCG (440 μM) was administered with first onset of ARIE and then at weeks after final radiotherapy. The patients were monitored every week for dysphagia, Radiation Therapy Oncology Group (RTOG) score, and esophagitis-related pain. Moreover, tumor response and the effect on survival following the treatment were also evaluated. Comparison of the RTOG score in the first, second, third, fourth, fifth, and even sixth week after EGCG prescription and the first and second week after radiotherapy with baseline indicates a significant reduction. The tumor response rate was 86.3%. The overall survival rate in 1, 2, and 3 years was found to be 74.5%, 58%, and 40.5%. Oral administration of EGCG solution seems to be feasible for treating ARIE in patients with esophageal cancer who receive radiation therapy. EGCG might be an ARIE-reliever without compromising the efficacy of radiation therapy. A randomized study with a control group is needed for further evaluation.

Transcriptome explores changes in gene expression profile contributed by Camellia sinensis (L.) in Apis cerana cerana and Apis mellifera ligustica

Zhao Dongxu,Chen Daoyin,Su Xiaoling,Zhang Xiaojun,Liu Yanjie 한국응용곤충학회 2022 Journal of Asia-Pacific Entomology Vol.25 No.3

Camellia sinensis (L.) is widely cultivated in China and other countries as an economically important tree and supplies unique nutrients to ensure the development of Apis cerana cerana and Apis mellifera ligustica colonies in early spring in southern China. A. c. cerana was domesticated in China many years ago and exhibits a smaller body size, smaller colony size and some distinct physiology and behavior compared to A. m. ligustica. Here, to explore the changes in gene expression induced by C. sinensis in A. c. cerana and A.m. ligustica, we performed transcriptomic analysis of the 1 st -instar, 5 th -instar, 1 st -adult and 7 th -adult stages of the two species fed the nectar and pollen of C. sinensis. A total of 489, 1190, 991 and 243 differentially expressed genes (DEGs) were identified in the four developmental stages of A. m. ligustica, and 995, 2785, 1513 and 213 DEGs were found in the cor responding stages of A. c. cerana. The changes in the gene expression profile were more significant in the 5 th -instar and 1 st -adult. Furthermore, the upregulated genes in the 5 th -instar and 1 st -adult were mainly enriched in pathways involved in growth- and development-related pathway: Hippo signaling pathway. In addition to the upregulated DEGs, growth- and development-related pathways, including the Wnt signaling pathway, Hedgehog signaling pathway-fly, and FoxO signaling pathway, were enriched in A. c. cerana. Finally, we hypothesized that C. sinensis pollen is beneficial to the growth and development of honeybees and give rise to diverse corresponding regulation to A. c. cerana than to A. m. ligustica.

Mitochondrial Location of Severe Acute Respiratory Syndrome Coronavirus 3b Protein

Yuwen Cong,Xiaoling Yuan,Yajun Shan,Zhenyu Yao,Jianyong Li,Zhenhu Zhao,Jiapei Chen 한국분자세포생물학회 2006 Molecules and cells Vol.21 No.2

Evaluation of Curtain Grouting Efficiency by Cloud Model - based Fuzzy Comprehensive Evaluation Method

Yushan Zhu,Xiaoling Wang,Shaohui Deng,Mengqi Zhao,Xuefei Ao 대한토목학회 2019 KSCE JOURNAL OF CIVIL ENGINEERING Vol.23 No.7

Because of the concealment and uncertainty of dam foundation grouting, the accurate evaluation of the quality of grouting construction is a major concern. Most of the previous studies pertaining to the assessment of grouting efficiency only take a single factor into account, and only a few efforts have been devoted to the comprehensive evaluation of grouting efficiency, which fail to consider the fuzziness problems caused by the complex relationships among indicators as well as the randomness problems caused by subjective consciousness. Therefore, the curtain grouting efficiency evaluation method considering the uncertainty in evaluation process is proposed which includes three main parts: 1) Based on conventional evaluation indicators of grouting efficiency, where groutability is introduced as a new indicator; 2) a fuzzy comprehensive evaluation method based on cloud model is developed, by which the fuzziness and randomness in assessment are organically combined; and 3) the fuzzy entropy was calculated for indicating the complexity of grouting efficiency level. Then, the proposed method is used to assess efficiency of the curtain grouting for a hydropower station in China, and compared with three other methods. The outcomes display the consistency, representativeness, robustness and superiority of this evaluation method, which make the evaluation results more scientific and objective.

Effects of dietary spermine supplementation on cell cycle, apoptosis, and amino acid transporters of the thymus and spleen in piglets

Cao, Wei,Wu, Xianjian,Jia, Gang,Zhao, Hua,Chen, Xiaoling,Wu, Caimei,Cai, Jingyi,Wang, Jing,Liu, Guangmang Asian Australasian Association of Animal Productio 2018 Animal Bioscience Vol.31 No.8

Objective: This study investigated whether spermine supplementation could regulate cell cycle, apoptosis, and amino acid transporter-related genes expression in the thymus and spleen of early weaned piglets. Methods: Eighty female piglets were randomly distributed to receive adequate nutrients supplemented with spermine (0.4 mmol/kg body weight/24 h) or to be provided with restricted nourishment supplemented with normal saline for 7 h or 3, 6, or 9 d in pairs. Results: Regardless of administration time, spermine supplementation significantly up-regulated cyclin A2 gene expression but down-regulated p21 and cyclin D3 mRNA levels in the thymus and spleen and reduced cyclin E2 gene expression in the thymus of piglets (p<0.05). Irrespective of the treatment period, the reduced Bax and caspase-3 gene expressions and improved Bcl-2 mRNA level were observed in the thymus and spleen of spermine-administrated piglets (p<0.05). Regardless of supplementation time, spermine intake significantly enhanced the expressions of amino acid transporter-related genes (SLC1A1, SLC1A5, SLC7A1, SLC7A7, and SLC15A1) in both thymus and spleen, as well as SLC7A9 in the spleen of piglets (p<0.05). In addition, extended spermine administration also markedly promoted cell proliferation, depressed apoptosis and modulated amino acid transport (p<0.05), and such effects were the greatest during prolonged spermine supplementation (6 d) compared to the other time periods (p<0.05). Conclusion: Spermine supplementation may regulate cell cycle during the G1/S phase, suppress apoptosis and modulate amino acid transport. A period of 6 d of spermine supplementation is required to produce the optimal effects on nutritional implications.

Modeling net energy requirements of 2 to 3-week-old Cherry Valley ducks

Yang Ting,Yu Lexiao,Wen Min,Zhao Hua,Chen Xiaoling,Liu Guangmang,Tian Gang,Cai Jingyi,Jia Gang 아세아·태평양축산학회 2020 Animal Bioscience Vol.33 No.10

Objective: A total of three hundred unsexed ducks were utilized to estimate net energy requirements of maintenance (NEm) and weight gain (NEg) for 2 to 3-week-old Cherry Valley ducks and to establish a model equation to predict NE requirements using the factorial method. Methods: To determine the apparent metabolizable energy (AME) of the diet, fifty 7-day-old ducks at approximately equal body weights (BWs) were randomly assigned into five groups that were fed at different levels (ad libitum, 85%, 75%, 65%, and 55% of ad libitum intake), and the endogenous acid-insoluble ash as indigestible marker. The two hundred and fifty 7-day-old ducks were used for a comparative slaughter experiment. At the beginning of the experiment, ten ducks were sacrificed to determine the initial body composition and energy content. The remaining ducks were randomly assigned into five groups (same as metabolic experiment). Ducks of the ad libitum group were slaughtered at 14 and 21-day-old. At the end of the experiment, two ducks were selected from each replicate and slaughtered to determine the body composition and energy content. Results: The results of the metabolizable experiment showed AME values of 13.43 to 13.77 MJ/kg for ducks at different feed intakes. The results of the comparative slaughter experiment showed the NEm value for 2 to 3-week-old Cherry Valley ducks was 549.54 kJ/kg of BW0.75/d, and the NEg value was 10.41 kJ/g. The deposition efficiency values of fat (Kf) and crude protein (Kp) were 0.96 and 0.60, respectively, and the values of efficiency of energy utilization (Kg) and maintenance efficiency (Km) were 0.75 and 0.88, respectively. Conclusion: The equation for the prediction of NE requirements for 2 to 3-week-old Cherry Valley ducks was the following: NE = 549.54 BW0.75+10.41 ΔW, where ΔW is the weight gain (g).