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Regulation of Nrf2 Transactivation Domain Activity by p160 RAC3/SRC3 and Other Nuclear Co-Regulators
Lin, Wen,Shen, Guoxiang,Yuan, Xiaoling,Jain, Mohit R.,Yu, Siwang,Zhang, Aihua,Chen, J. Don,Kong, Ah-Ng Tony Korean Society for Biochemistry and Molecular Biol 2006 Journal of biochemistry and molecular biology Vol.39 No.3
Transcription factor NF-E2-related factor 2 (Nrf2) regulates the induction of Phase II detoxifying enzymes and antioxidant enzymes in response to many cancer chemopreventive compounds. In this study, we investigated the role of receptor associated coactivator (RAC3) or steroid receptor coactivator-3 (SRC3) and other nuclear co-regulators including CBP/p300 (CREB-binding protein), CARM1 (Coactivator-associated arginine methyltransferase), PRMT1 (Protein arginine methyl-transferase 1), and p/CAF (p300/CBP-associated factor) in the transcriptional activation of a chimeric Gal4-Nrf2-Luciferase system containing the transactivation domain (TAD) of Nrf2 in HepG2 cells. The results indicated that RAC3 up-regulated the transactivation activity of Gal4-Nrf2-(1-370) in a dose-dependent manner. The enhancement of transactivation domain activity of Gal4-Nrf2-(1-370) by RAC3 was dampened in the presence of dominant negative mutants of RAC3. Next we studied the effects of other nuclear co-regulators including CBP/p300, CARM1, PRMT1 and p/CAF, and the results showed that they had different level of positive effects on this transactivation domain activity of Gal4-Nrf2-(1-370). But importantly, synergistic effects of these co-regulators in the presence of RAC3/SRC3 on the transactivation activity of Gal4-Nrf2-(1-370) were observed. In summary, our present study showed for the first time that the 160 RAC3/SRC3 is involved in the functional transactivation of TAD of Nrf2 and that the other nuclear co-regulators such as CBP/p300, CARM1, PRMT1 and p/CAF can also transcriptionally activate this TAD of Nrf2 and that they could further enhance the transactivation activity mediated by RAC3/SRC3.
Behavior Characteristics of Hydrogen and its Isotope in Molten Salt of LiF-NaF-KF (FLiNaK)
Youshi Zeng,Wenguan Liu,WeiLiu,Yuan Qian,Nan Qian,Xiaoling Wu,Yu Huang,Shengwei Wu,Guanghua Wang 한국원자력학회 2019 Nuclear Engineering and Technology Vol.51 No.2
Experimental studies to investigate the behaviors of hydrogen in molten FLiNaK (LiF-NaF-KF) have beenconducted at 500e700 C. On the basis of previous studies, the diffusivity and solubility of hydrogen inFLiNaK have been revised, and the expressions can be correlated to the following Arrhenius equations:DH2 ¼ 1.62 10 5exp ( 48.20 103/Rg$T) [m2/s] and SH2 ¼ 1.03 10 4exp ( 14.96 103/Rg$T) [mol-H2/m3/Pa], respectively. The behavior characteristics of deuterium in FLiNaK were studied and comparedwith the hydrogen behaviors in FLiNaK. The results showed the behaviors of deuterium were consistencewith the behaviors of hydrogen in FLiNaK. The difference between hydrogen and deuterium has not beenobserved upon the experimental research of the behavior characteristics of hydrogen and deuterium inFLiNaK, which suggested the results obtained here might apply equally to the behavior characteristics oftritium in FLiNaK.
Polymorphisms in the Perilipin Gene May Affect Carcass Traits of Chinese Meat-type Chickens
Zhang, Lu,Zhu, Qing,Liu, Yiping,Gilbert, Elizabeth R.,Li, Diyan,Yin, Huadong,Wang, Yan,Yang, Zhiqin,Wang, Zhen,Yuan, Yuncong,Zhao, Xiaoling Asian Australasian Association of Animal Productio 2015 Animal Bioscience Vol.28 No.6
Improved meat quality and greater muscle yield are highly sought after in high-quality chicken breeding programs. Past studies indicated that polymorphisms of the Perilipin gene (PLIN1) are highly associated with adiposity in mammals and are potential molecular markers for improving meat quality and carcass traits in chickens. In the present study, we screened single nucleotide polymorphisms (SNPs) in all exons of the PLIN1 gene with a direct sequencing method in six populations with different genetic backgrounds (total 240 individuals). We evaluated the association between the polymorphisms and carcass and meat quality traits. We identified three SNPs, located on the 5' flanking region and exon 1 of PLIN1 on chromosome 10 (rs315831750, rs313726543, and rs80724063, respectively). Eight main haplotypes were constructed based on these SNPs. We calculated the allelic and genotypic frequencies, and genetic diversity parameters of the three SNPs. The polymorphism information content (PIC) ranged from 0.2768 to 0.3750, which reflected an intermediate genetic diversity for all chickens. The CC, CT, and TT genotypes influenced the percentage of breast muscle (PBM), percentage of leg muscle (PLM) and percentage of abdominal fat at rs315831750 (p<0.05). Diplotypes (haplotype pairs) affected the percentage of eviscerated weight (PEW) and PBM (p<0.05). Compared with chickens carrying other diplotypes, H3H7 had the greatest PEW and H2H2 had the greatest PBM, and those with diplotype H7H7 had the smallest PEW and PBM. We conclude that PLIN1 gene polymorphisms may affect broiler carcass and breast muscle yields, and diplotypes H3H7 and H2H2 could be positive molecular markers to enhance PEW and PBM in chickens.