겨울철 제주연안 열수지 특성 : 강설현상 snowfall

김경보,장승민,김태희,우종규,윤용훈 濟州大學校 海洋硏究所 2004 해양과환경연구소 연구논문집 Vol.28 No.1

In other to know the characteristics of the air sea heat flux at on the coastal of Jeju Island. when there was a snowfall in winter. It is used as the basic data are Automatic Synoptic Observation System(ASOS) compiled by Jeju Regional Meteorological Office(JRMO) from 1993 to 2002, and SST data derived from National Oceanographic Research Institute(NORI) and National Fisheries Research & Development Institute(NFRDI) observations. the heat flux of the coastal of Jeju Island shows its maximum in January(latent heat flux : 35Wm^(-2) , sensible heat flux : 85Wm^(-2)), and shows its minimum in July(latent heat flux : -l3Wm^(-2), sensible heat flux : -5Wm^(-2)) the more the temperature gap between the air and SST is large and the stronger a wind is, the more it supplies the heat from the sea to the air. it shows that the north-western areas is about three times higher than other areas in Jeju Island. when fresh snow cover is 0.0∼0.9 ㎝, the mean bowen's ratio(for 1993∼2002) is 0.63∼0.67. when it is 1.0∼4.9 ㎝, the mean bowen's ratio(for 1993∼2002) is over 0.72 or more. When bowen's ratio increased upto 0.63∼0.80. fresh snow cover is increased gradually, therefore, it is researched the there is proportion between the fresh snow cover and the bowen's ratio.

扶正抗癌湯의 抗腫瘍效果에 關한 實驗的 硏究

文錫哉,文九,金柄住,田炳薰,元秦喜 대한동의병리학회 1997 동의생리병리학회지 Vol.11 No.2

腫瘍으로 인한 死亡率은 多樣한 名種 治療에도 不拘하고 循環器疾患 다음으로 2위를 차지하고 있다. 이에 著者는 韓醫學과 西醫學의 結合治療를 通해서 抗腫瘍藥物의 效果를 增進시키고 副作用을 줄이는 藥物의 開發을 爲하여 健脾 益氣 祛痰 補腎 杭癌效能을 갖고 잇는 扶正杭癌湯抽出液을 使用하여 Colony 形成抑制實驗, Sulforhoda-mine B(SRB) assay를 觀察하였고, 扶正抗癌湯抽出液과 抗癌化學療法劑인 Mitomycin C(MMC)를 Ehrlich carcinoma의 solid tumor와 ascites tumor에 各各 單獨 및 倂用 投與하여 抗腫瘍效果 및 Iysosomal enzymes의 活性에 대한 效果를 觀察하였다. 먼저 扶正抗癌湯抽出液을 Caki-1 cell, Hep 3B 및 A549에 투여한 후 增殖抑制作用을 colony 形成抑制實驗과 SRB assay를 통하여 觀察한 결과 濃度依存的으로 腫瘍細胞의 成長을 抑制하였다. Ascites form of Ehrlich carcinoma에 대한 抗腫瘍效果 實驗에서 扶正抗癌湯抽出液과 MMC를 병용투여한 결과, 扶正抗癌湯抽出液에 의하여 MMC의 抗腫瘍效果가 增加하였다. Solid form of Ehrlich carcinoma에 대한 抗腫瘍效果 實驗에서 扶正抗癌湯抽出液과 MMC를 병용 투여하였을 경우, MMC를 단독 투여하였을 경우보다 腫瘍의 크기가 현저하게 減少하는 결과를 보였다. Ehrlich ascites carcinoma cell에 扶正抗癌湯抽出液과 MMC를 병용 투여하였을 경우, MMC를 단독 투여하였을 경우보다 lysosomal enzymes의 活性이 强하게 나타났다. 本 實驗을 통하여 扶正抗癌湯抽出液은 MMC의 抗腫瘍效果를 증가시키는 效果를 보였다. 또한 扶正抗癌湯抽出液은 마우스에서 solid form of Ehrlich ascites carcinoma의 크기를 감소시키는 效果를 나타냈으며, 생존기간 實驗에서는 扶正抗癌湯抽出液과 MMC를 병용투여시 ascites tumor에 MMC를 단독으로 투여하였을 때와 비교하여 유의하게 생존기간을 증가시키는 결과를 보였다. 이상의 결과를 종합하여 볼 때 扶正抗癌湯抽出液은 用量에 依存的으로 直接的인 抗腫瘍效果가 있으며, 抗癌療法劑인 MMC의 效果를 亢進시키는 것으로 보아 間接的인 抗腫瘍效果도 있을 것으로 思科되나 正確한 效果를 究明하기 위해서는 보다 正確한 抗腫瘍機轉에 대한 硏究와 藥物學的 硏究 및 臨床的 硏究가 追加되어야 할 것이다. In order to investigate the effects of Bujeonghangamtang Extract(扶正抗癌湯抽出液) on antitumor effects after human cell lines(A549, Hep3B, Caki-1, Ehrlich) transplantation into the peritoneal cavity or right groin in mice induced by RPMI 1640 and DMEM etc, the extracts of its herbal medicines were orally administered for 10 or 12 days. Experimental studies were performed for measurement of antitumor effect of Mitomycin C(MMC) and lysosomal enzyme's activities using colony forming efficiency, SRB assay which were regarded as a valuable method for the measurement of antitumor effects of unknown compound on tumor cell lines. The results obtained in this studies were as follows: 1. The change of colony-forming efficiency and SRB assay of Caki-1 cells, Hep3B and A549 Cells after exposure to the extract of Bujeonghangamtang extract depressed the growth of tumor cells by concentration of Bujeonghangamtang. 2. Antitumor activity of the ethanol extract of Bujeonghangamtang extract and MMC on ascites form of Ehrlich carcinoma in mice is slightly improved. Especially the mean of survival times in the group of 200㎎/㎏ and MMC 0.1㎎/㎏ is improved over 30.9%. 3. When Bujeonghangamtang extract and MMC are administered together, the weight of tumor is more decreased than MMC alone. 4. The lysosomal enzyme's activities of the Bujeonghangamtang extract and MMC are more significantly improved than MMC alone. According to the above result, it could be suggested that Bujeonghangamtang extract has indirect antitumor effect by the increase of MMC uptake.

최대근력부하와 폭발적 근력부하에 의한 신경-근피로와 그 회복과정 비교 분석

유관호,이무연,이승훈,김민섭,육조영,임정일,임완기,김대경 한국스포츠리서치 2004 한국 스포츠 리서치 Vol.15 No.5

The result which was gained from this study is as follows. From the conclusion of this study it was confirmed that resistant motion at high level strength brings about rise in density of lactate in blood at the same time decline of muscular power and generates acute fatigue of nerve-muscle accompanied by decline in electrical activity of muscle. On the other hand explosive loading produced fatigue at body center and decline in nervine-muscular activity particularly for men, In case of women explosive load did not produce fatigue in the same level as men and they were also quicker in recovery after exercise. Based on 2 types of loading method used in this study there was clearly difference in the process of restoration from resistant motion. For greater decline in muscular power and electromyogram activity more longer period of recovery was require.

Highly selective adsorption of SF₆ over N₂ in a bromine-functionalized zirconium-based metal-organic framework

Kim, Min-Bum,Kim, Kyung-Min,Kim, Tea-Hoon,Yoon, Tae-Ung,Kim, Eun-Jung,Kim, Jeong-Hoon,Bae, Youn-Sang Elsevier 2018 CHEMICAL ENGINEERING JOURNAL -LAUSANNE- Vol.339 No.-

<P><B>Abstract</B></P> <P>We have systematically synthesized a series of functionalized UiO-66-X (X = NO<SUB>2</SUB>, NH<SUB>2</SUB>, Cl, Br, I, Br<SUB>2</SUB>) compounds to further improve the selective SF<SUB>6</SUB> adsorption of UiO-66, which is one of the best-performing adsorbents, and to investigate the effect of the polarizability of adsorbent atoms on the SF<SUB>6</SUB> and CF<SUB>4</SUB> adsorption, especially at low pressures, which is the industrially relevant condition. UiO-66-Br<SUB>2</SUB> with the highest polarizability exhibited a high Henry’s constant (104 mmol g<SUP>−1</SUP> bar<SUP>−1</SUP>) for SF<SUB>6</SUB> adsorption as well as high SF<SUB>6</SUB>/N<SUB>2</SUB> selectivity (200), both of which are considerably higher than those of other adsorbent materials, including MOFs. Moreover, UiO-66-Br<SUB>2</SUB> showed good SF<SUB>6</SUB>/N<SUB>2</SUB> separation under a dynamic mixture flow condition as well as facile regeneration without increasing the temperature. These results show that UiO-66-Br<SUB>2</SUB> is a potential adsorbent for the selective capture of SF<SUB>6</SUB> from various SF<SUB>6</SUB>/N<SUB>2</SUB> mixtures in industry.</P> <P><B>Highlights</B></P> <P> <UL> <LI> A series of UiO-66-X (X = NO<SUB>2</SUB>, NH<SUB>2</SUB>, Cl, Br, I, Br<SUB>2</SUB>) was synthesized. </LI> <LI> UiO-66-Br<SUB>2</SUB> with the highest polarizability exhibits the highest SF<SUB>6</SUB>/N<SUB>2</SUB> selectivity values ever reported. </LI> <LI> UiO-66-Br<SUB>2</SUB> shows good SF<SUB>6</SUB>/N<SUB>2</SUB> separation under a dynamic mixture flow condition. </LI> <LI> UiO-66-Br<SUB>2</SUB> can be easily regenerated by pure N<SUB>2</SUB> without increasing the temperature. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

Cloning and Characterization of the Lactococcus lactis subsp.lactis ATCC 7962 ptsHI Operon

Kim, Tea Youn,Park, Rae Jun,Chang, Hae Choon,Chung, Dae Kyun,Lee, Jong Hoon,Lee, Hyong Joo,Kim, Jeong Hwan 한국미생물 · 생명공학회 2000 Journal of microbiology and biotechnology Vol.10 No.6

The ptsH and ptsl genes of Lactococcus lactis subsp. lactis ATCC 7962 (L. lactis 7962), encoding the general proteins of phosphotransferase system (PTS) components, HPr and enzyme I, respectively, were cloned and characterized. A 1.3kb PCR product was obtained using a primer set that was hybridized to the internal region of the L. lactis 7962 ptsHI genes and then subcloned into a low-copy number vector, pACYC 184. The 5' upstream and 3' downstream regions from the 1.3kb fragment were subsequently cloned using the chromosome walking method. The complete ptsHI operon was constructed and the nucleotide sequences determined. Two ORFs corresponding to HPr (88 amino acids) and enzyme I (575 amino acids) were located. The ptsHl genes of LL lactis 7962 showed a very high homology (84-90%) with those genes from other Gram-positive bacteria. A primer extension analysis showed that the transcription started at either one of two adjacent bases upstream of the start codon. Using a Northern analysis, two transcripts were detected; the first, a 0.3kb transcript corresponding to ptsH and the second, a 2kb transcript corresponding to ptsH and ptsl. The transcription level of ptsH was higher than that of ptsl. The concentration of the ptsH transcript in cells grown on glucose was similar to that in cells grown on lactose, yet higher than that in cells grown on galactose. The ptsl transcript was scarcely detected in cells grown on lactose or galactose. The results of a sequence analysis and Northern blot confirmed that the ptsH and ptsI genes of L. lactis 7962 were arranged in an operon like other known ptsHI genes and the expression of the ptsHI genes was regulated at the transcriptional level in response to the carbon source.

최근 국내 유입 Vibrio cholerae균 및 1995년도 국내 집단 발생 콜레라의 역학적 양상

김호훈,신영학,강연호,유천권,박미선,김동술,유재연,전정훈,이복권,박기덕,김동진,정태화,이종구,박기동,김상순,이동모,김문식,조병륜 대한감염학회 1996 감염 Vol.28 No.6

목적 : 1992년 이후 국내에 유입, 확인되는 V. cholerae 균은 현격한 증가 추세를 보이고 있으며, 1995년도 국내 집단 발생 콜레라의 역학적 양상이 종래의 경우와 상이한 바 있어 최근의 콜레라균 유입상황과 국내 역학적 특성을 정리하여 향후의 방역 대책에 참고로 하기 위하여 본 연구를 시행하였다. 방법 : 국내 유입 V. cholerae 균은 세균학적 방법에 의해 1986-1995년 사이에 확인된 균주를 대상으로 분석하였고, 국내 집단발생 콜레라는 1995년도에 내국인 거주자에게서 진성 콜레라 환자로 구명된 환자를 대상으로 역학적 양상을 살펴보았다. 결과 : 국내 유입 V. cholerae균은 1992년 이후 1995년 사이에 계절과 무관하게 뚜렷한 증가 추세를 보였다. 1995년도 콜레라 국내 집단 발생의 역학적 양상은 초발 환자가 인지되면서 단기간 사이에 지역적 연고가 없는 다양한 지역에서 콜레라 환자 발생이 확인되었다. 환자 발생 지역 내에서는 비교적 소규모 환자 발생에 그쳤고 진성 콜레라 환자로 확인된 환자 중 사망자는 없었으며 남성에서 여성보다 발병율이 높았다. 결론 : V.cholerae O1균의 국내 유입이 최근 현격히 증가되었고, 1994년에는 V. cholerae O139균의 국내유입이 처음으로 확인되었다. 콜레라 환자의 국내 집단 발생 위험은 1992년 이후 증가되었으며 1995년도의 집단 환자 발생은 지역적 연고가 없는 다양한 지역에서 비교적 단기간에 사망자 없이 소규모의 환자 발생양상을 보였다. Background: Imported Vibrio cholerae strains have increasingly been found since 1992 and the pattern of recent cholera epidemic outbreak in Korea showed a little difference from that of former epidemic outbreak. Methods: We had collected suspected V. cholerae isolates from Quarantine Stations or Public Health Laboratories, which were identified as V. cholerae O1 or O139. Epide miological analysis was made on the base of field surveys. Results: During the period from 1986 through 1995, 138 V. cholerae strains were imported. Nearly 85.5% of the strains (118 strains) were imported within recent 4 years from 1992 to 1995. One hundred and twenty-eight strains were E1 Tor-Ogawa type V. cholerae O1, 6 strains were V. cholerae O139 and 4 strains were E1 Tor-Inaba type V. cholerae O1. Of 138 strains, 71 strains were isolated from the airplane toilet swab and 67 strains from the passengers who entered into Korea after international travel. From 1993 to 1995, 101 strains were imported from the Southeast Asian countries; including Thailand(65), Indonesia(17), Philippines(10), Hongkong(6) Singapore(2) and Vietnam(1). During the cholera epidemic in 1995, 68 patients with V. cholerae O1 (E1 Tor-Ogawa) were found. Oneset of the index-case occurred on August 26th and the last on September 13th. Geographically it occurred in Incheon city(25), Chungcheongnam do(25), Kangwon do(6), Kyungsangbuck do(5), Kyunggi do(4), Daejeon city(2) and Pusan city(1). Of total 68 cases, 39 were male. Cases were found in all age groups, but it was more common in elderly groups. During the epidemic, E1 Tor-Ogawa type V. cholerae O1 strains were also isolated from the coastal sea water of Kanghwa kun where the epidemic outbreak occurred. Conclusions: In Korea, the imported V. cholerae O1 and V. cholerae O139 strains have increasingly been found without seasonal variation since 1992. Cholera epidemic outbreak in 1995, occurred simultaneously at many different local areas within a short period without mortality.

Observation of Olefin/Paraffin Selectivity in Azo Compound and Its Application into a Metal-Organic Framework

Kim, Seo-Yul,Yoon, Tae-Ung,Kang, Jo Hong,Kim, Ah-Reum,Kim, Tea-Hoon,Kim, Seung-Ik,Park, Wanje,Kim, Ki Chul,Bae, Youn-Sang American Chemical Society 2018 ACS APPLIED MATERIALS & INTERFACES Vol.10 No.32

<P>Olefin/paraffin separation is an important and challenging issue because the two molecules have similar physicochemical properties. Although a couple of olefin adsorbents have been developed by introducing inorganic nanoparticles into metal-organic frameworks (MOFs), there has been no study on the development of an olefin adsorbent by introducing a certain organic functional group into a MOF. In this study, we posited that azo compounds could offer olefin/paraffin selectivity. We have revealed using first-principles calculations that the simplest aromatic azo compound (azobenzene, Azob) has an unusual propylene/propane selectivity due to special electrostatic interactions between Azob and propylene molecules. On the basis of this interesting discovery, we have synthesized a novel propylene adsorbent, MIL-101(Cr)_DAA, by grafting 4,4′-diaminoazobenzene (DAA) into open metal sites in a mesoporous MIL-101(Cr). Remarkably, MIL-101(Cr)_DAA exhibited enhanced propylene/propane selectivity as well as considerably higher propylene heat of adsorption compared to pristine MIL-101(Cr) while maintaining the high working capacity of MIL-101(Cr). This clearly indicates that azo compounds when introduced into MOFs can provide propylene selectivity. Moreover, MIL-101(Cr)_DAA showed good C<SUB>3</SUB>H<SUB>6</SUB>/C<SUB>3</SUB>H<SUB>8</SUB> separation and easy regeneration performances from packed-bed breakthrough experiments and retained its propylene adsorption capacity even after exposure to air for 12 h. As far as we know, this is the first study that improves the olefin selectivity of MOF by postsynthetically introducing an organic functional group.</P> [FIG OMISSION]</BR>

토석류 표면유속 측정을 위한 딥러닝을 이용한 비접촉식 STIV 기법의 적용

김연중(Kim, Yeon Joong),김태우(Kim, Tea Woo),윤종성(Yoon, Jong Sung),정재훈(Jeong, Jae Hoon) 대한토목학회 2020 대한토목학회 학술대회 Vol.2020 No.10

Efficient SF6/N2 separation at high pressures using a zirconium-based mesoporous metal–organic framework

Min-Bum Kim,Tea-Hoon Kim,Tae-Ung Yoon,Jo Hong Kang,Jeong Hoon Kim,Youn-Sang Bae 한국공업화학회 2020 Journal of Industrial and Engineering Chemistry Vol.84 No.-

Adsorptive separation of SF6/N2 mixtures is an important issue since SF6 is a significant greenhouse gas. Although separation performances should be evaluated under high pressure conditions tofind anefficient adsorbent candidate for pressure swing adsorption (PSA) processes, no studies on metal–organicframeworks (MOFs) have focused on SF6/N2 separation at high pressures above 1 bar. In this study, weevaluated the potential of three hydrothermally stable adsorbents, including two MOFs (UiO-66 andUiO-67) and zeolite-13X, for SF6/N2 separation under normal PSA operation pressures ( 10 bar). Interestingly, UiO-67 with a high surface area and large pore size exhibited very high SF6/N2 selectivity(30 37) at 10 bar as well as a significantly large SF6working capacity (5.94 mmol g 1) between 10 bar and1 bar. Moreover, UiO-67 showed facile regeneration at the ambient temperature as well as good cyclicadsorption and desorption behavior during 20 cycles. These results show that UiO-67 is a potentialadsorbent for adsorptive SF6/N2 separation.

Fast and Efficient Isolation of Mouse Bone Marrow-Derived Mesenchymal Stem Cells by Using a Biocompatible Polymer

( Han Soo Kim ),( June Seok Heo ),( Jung Mok You ),( Tea Hoon Park ),( You Jeong Choi ),( Eunk Young Kim ),( Hyun Ok Kim ) 한국조직공학과 재생의학회 2010 조직공학과 재생의학 Vol.7 No.4

Mesenchymal stem cells (MSCs) differentiate into bone, fat, cartilage, tendon, and other organ progenitor cells. The rarity of MSCs in bone marrow necessitates fast and efficient isolation and/or in vitro expansion prior to clinical and biomedical applications. Previously, we reported that UV-exposed diphenylamino-s-triazine bridged p-phenylene vinylene (DTOPV-UV) with a hydrophilic and negative surface-containing carboxyl group is highly biocompatible and provides a substrate for efficient human bone marrow-derived MSC attachment. In this study, we applied this polymeric film to early adhesion and enrichment of MSCs from mouse bone marrow. With its high protein- binding capacity, DTOPV-UV film was more efficient in early capture of adherent bone marrow cells than conventional tissue culture polystyrene (TCPS). Cell binding to DTOPV-UV reached full capacity within 1 hr, whereas cell attachment to TCPS gradually increased over time. The isolated and culture-expanded MSCs from mouse bone marrow displayed typical morphology, phenotype, and differentiation into osteoblasts, adipocytes, and chondrocytes. Here, we demonstrate a novel method for isolating MSCs from mouse bone marrow using a biocompatible polymer. This method will aid the development of rapid and efficient isolation and in vitro expansion protocols for rare adherent cells.