당뇨병 백서의 간세포에서 Glucokinase 활성도 및 유전자 발현에 대한 인슐린의 영향

강성이,팽정령,서광식,안규정,우정택,김성운,양인명,김진우,김영설,김광원,최영길 경희대학교 유전공학연구소 1993 遺傳工學論文集 Vol.5 No.-

목적 당대사의 조절 상태에 따른 생체 변화를 분자 수준에서 이해하고자 식이 조건을 달리한 정상 백서와 화학적으로 유도된 당뇨병 백서의 간조직에서 혈당수준과 인슐린치료 정도에 따라 나타나는 글루코키나제 활성도 및 유전자 발현을 분석하였다. 방법 스트렙토조토신 정맥투여 후 당뇨병의 유발을 확인하고, 인슐린을 1일 3회 3일간 복강내로 투여하여 상태를 안정시킨 후, 인슐린 투여군은 인슐린 투여 6시간이내에 그리고 인슐린 비투여군은 24시간 후 단두하여 채혈하고 복강을 열어 간조직을 채취하였다. 채취한 간조직에서 글루코키나제 활성도는 인산화된 포도당에서 NADH의 형성을 형광분광계로 측정하였으며, 글루코키나제 유전자 mRNA발현은 Northern 분석법을 이용하였다. 성적 정상 백서에서 공복상태와 식이를 섭취한 경우에 간조직의 글루코키나제 효소의 활성은 차이가 없었으나, 글루코키나제 유전자 mRNA 발현은 증가되었다. 당뇨병이 유발된 백서의 간조직에서 글루코키나제 효소의 활성 및 글루코키나제 유전자의 mRNA 발현은 정상 백서에 비하여 낮았다. 인슐린 투쳐 후 글루코키나제 효소의 활성 및 글루코키나제 유전자의 mRNA 발현이 증가되었고, 특히 혈당이 정상화된 경우에서 글루코키나제 유전자의 mRNA 발현이 증가도었다. 결론 인슐린에 의한 간조직에서 글루코키나제 효소의 활성 및 글루코키나제 유전자의 mRNA을 증가를 볼수 있었다. 당뇨병 백서에서 인슐린 투여 후에 혈당조절이 안된 경우 간조직의 글루코키나제 유전자의 mRNA 발현이 증가가 없는 것으로 보아 글루코키나제 mRNA의 발현에는 인슐린 이외의 다른 요소가 관여할 것으로 생각된다. The liver-specific hexokinase isoenzyme, referred to as glucokinase, is thought to play a key reglulatory role in hepatic glucose metabolism. The glucokinase gene is, therefore, of interest both because of its tissue-specific expression and because of the several regulatory processes that can be analyzed. The level of hepatic glucokinase activity appears to be determined essentially by regulation of the rate of enzyme synthesis, with insulin playing a leading role as an inducer. We investigated the role of insulin for the induction of glucokinase in the liver of diabetic rats. Experimental diabetes was induced by injection of streptozotocin 7 days before the experiment. Regular insulin was given by three days intraperitoneal injection at 8-h interval. The glucokinase mRNA in the liver was estimated by Nothern blot assay, as well as by fluorometric enzyme activity assay. Glucokinase activity was not reduced in the liver of normal fasting rats as compared to normal fed rats. And glucokinase activity was reduced in the liver of diabetic rats as compared to normal rats. In diabetic rats treated with insulin, glucokinase enzyme activity were increased. But glucokinase mRNA expression was only increased in normoglycemic diabetic rat with treated with insulin as compared to hyperglycemic rat. These data indicate that insulin stimulates hepatic glucokinase enzyme activity and mRNA expression. But other hormonal or metabolic factors may be contribute to regulation of glucokinase mRNA expression.

기능적 자기공명 영상법의 이해

서정진,정광우,강형근 全南大醫大 2003 전남의대학술지 Vol.39 No.4

폐광산 주변의 목본 식생 현황 및 오염 토양에 대한 목본 종자의 발아 특성

서경원,손요환,김래현,구진우,노남진,경지현,김정규 한국환경농학회 2006 한국환경농학회지 Vol.25 No.1

정신과적 관점에서 본 임신과 출산 : The Psychiatric Perspectives

서정석,김소정,남범우 건국대학교 의과학연구소 2004 건국의과학학술지 Vol.13 No.-

The pregnancy and delivery are the most significant and valuable life events at any women regardless of a race, culture, social status, and religion. Psychiatric disorders in women occur most frequently between the ages of 18 and 45 years, which these years are the childbearing period. Pregnant women have many changes, such as cravings, aversive behaviors, pica, sickness, changes in taste and smelling and pseudocyesis. Also, the postpartum period represents a time of heightened risk for the onset or worsening of mood disorder and other mental disorder. Among these psychiatric disorders, postpartum mood syndrome are generally classified as postpartum blue, postpartum depression, and postpartum psychosis. So, we reviewed the changes during pregnancy and the postpartum mood syndrome in view of psychiatric perspectives.

과학고 교육과정 운영에 대한 과학교사 및 학생의 인식

서혜애,손정우 한국교육과정학회 2007 교육과정연구 Vol.25 No.2

양극성 장애의 우세삽화와 임상양상 간의 연관성 : 예비연구

서호준,우영섭,채정호,전태연,김광수,박원명 大韓神經精神醫學會 2007 신경정신의학 Vol.46 No.5

Objective : This study was performed to fmd clinical implications ofdistinctive pattems in bipolar patients with predominance of depressive episodes or manic episodes and to find possibilities to predict predominant episodes in bipolar disorder. Methods : The recmitment period was between 2002 and 2004, among inpatients who had received psychiatric treatment for bipolar disorder. The data about the number and the polarity of the past episodes were collected through medical records, and Sub-jects were divided into depressive or manic episode predominant groups. The two groups were compared regarding Sociodemo-graphic and clinical variables. Results : 74 patients met the inclusion criteria during the study period. 21 subjects were classified as depressive episode Pre-dominant group (depressive episode group), and 53 subjects as manic episode predominant group (manic episode group). There were no differences in sociodemographic data, the number of total episodes and hypomanic episodes, and onset age. However, 90.5% (n=19) subjects of depressive episode group showed depressive episode as first episode, and 88.7% (n=47) of manicepisode group experienced manic episode as first episode (χ²=42.13, df=1, P<0.00l). Psychotic symptomswere significantly more prominent in the manic episode group (χ²=6.85, df=1, P=0.009), and the number ofsubjects who had suicide attempt experience was higher in the depressive episode group (χ²=7.39, df=1, P=0.007). In the comparison of highest GAF scores during the past year, significant difference was found between the two group (p=0.046), as the mean scores of depressive epi-sode group was 68.337±7.47 and manic episode groupwas 71.51±5.42. Conclusion : The results ofthis study show that there are differences in clinical features according to predominant episodes in bipolar disorder. The type of onset episode appears to be related to predominant episode in this study, and it would be a valid Pa-rameter with therapeutic implication in bipolar patients.

제한효소 DNA 분석법에 의한 국내분리 렙토스피라균의 동정

장우현, 김석용, 서정선 忠北大學校 遺傳工學硏究所 1988 遺傳工學硏究 Vol.2 No.1

본문참고

조기 발병 양극성 장애 환자의 임상적 특징 : 후향적 의무기록 조사 연구

우영섭,박민현,서호준,채정호,전태연,박원명 大韓神經精神醫學會 2007 신경정신의학 Vol.46 No.5

Objective : Bipolar disorder is a disease with diverse clinical profiles and outcomes. In recent years, it is suggested that if bipolar disorder occurs early in one's life, functional and symptomatic prognoses are poor. The prognoses include severe Symp-toms, frequent psychotic symptoms, comorbidity ofmental illnesses, slow improvement of the symptoms, and high suicide rate. We investigated the clinical characteristics of early onset bipolar inpatients. Method : The subjects of this study were selected from the patients who were discharged after hospitalization between January 1, 2001 and May 31, 2005 and diagnosed with bipolar disorder according to DSM-IV criteria. These patients were examined for the following data ; the type of bipolar disorder, comorbid psychiatric disorders, the age at onset of the illness, the acuteness of the disorder, and the duration of the disorder. The presence of four major clinical psychopathology, rapid cycling, Psychotic symptoms, comorbid psychiatric disorders, and suicide attempt, was also examined. The patients whose onset of the illness was before the age of 19 were classified as the early onset group and after the age of 19 were classified as the late onset group. Data on demographics, family histories, four major clinical psychopathologies and other clinical variables were compared between the early onset group and the late onset group. Results : Of the 53 patients, 19 patients belonged to the early onset group. There was higher incidence ofpsychotic Symp-toms in the early onset group than in the late onset group, and the difference was statistically significant according to the χ² - test (84.2% vs 44.1%, P=0.005). The incidence of comorbid psychiatric disorders was higher in the early onset group than in the late onset group, and the difference was also statistically significant according to the χ²-test (52.6% vs 23.5%, P=0.032).However, there was no statistically significant difFerence between the groups regarding the incidence of rapid cycling and suicide attempt. The result of logistics regression showed correlation of psychotic symptoms (0R=6.756 ; 1.655≤95% CI≤27.580) and comorbid psychiatric disorders (0R=3.611 ; 1.088≤95% CI≤11.984) with the early onset group. Conclusion : Early onset of bipolar disorder is related to the manifestation of psychotic symptoms and frequently accom-panies comorbid psychiatric disorders. The results of this study will help understand the mechanism of the onset of complexbipolar disorder and estimate the prognosis.

위장관의 원발성 B 세포 악성 림프종의 재분류와 bcl-2, cyclin D1, bcl-6 및 p53 발현의 의미

서정균,김태원,김병수,조은택,박찬국,김만우,이미자,전호종 조선대학교 2001 The Medical Journal of Chosun University Vol.26 No.2

Background and Objectives : In recent years, the REAL (Revised European American Lymphoma) classification provided a new category of MALT (mucosa-associated lymphoid tissue) lymphoma and mantle cell lymphoma in B cell lymphomas. We have analyzed the expression of bcl-2, cyclin D1, bcl-6 and p53 and correlated with the subtypes and histologic grade of primary gastrointestinal B cell lymphoma. Also we investigated the usefulness of immunophenotypic features in diagnosis of low grade B cell lymphoma. Materials and Methods : Twenty-two cases of primary gastrointestinal B cell lymphoma were recategorized in low grade MALT lymphoma, low/high grade MALT lymphoma, high grade MALT lymphoma, and diffuse large cell lymphoma according to the morphological findings. We investigated the expression of bcl-2, cyclin D1, bcl-6 and p53 by immunohistochemical method. Results : The bcl-2 protein expression was higher in the low grade MALT lymphoma than in the high grade lymphoma. The cyclin D1 protein expression was negative in all cases. The expression of bcl-6 and p53 protein was negative in all low grade MALT lymphoma. Conclusion : The results suggest that we can differentiate the low grade lymphoma from the high grade lymphoma by immunohistochemical staining for bcl-2, bcl-6, and p53 protien.

정신과 폐쇄 병동 환경 평가에 영향을 미치는 임상변인 : 치료의 질적 향성을 위한 예비적 연구 The Preliminary Study for Promoting the Quality of Psychiatric Inpatient Treatment

서정석,류은정,이상미,한미희,최귀순,손인기,남범우 건국대학교 의과학연구소 2003 건국의과학학술지 Vol.13 No.-

Purpose: IS prelminary study was designed to assess the clinical variables influencing on the ward atmosphere in psychiatric closed ward and to promote the quality of psychiatric inpatient treatment. Method: 41 psychiatric inpatients were selected in Chung-ju hospital, Konkuk university. Ward Atmosphere Scale(W AS) was used to evaluate patients' perception of ward milieu. WAS score were compared among subgroups with respect to the clinical variables such as duration of admission, frequency of admission, and diagnosis. Results: Significantly, patients who have hospitalized for 1 month to 2 months reported lower score of WAS and patients who have hospitalized for longer than 2 months reported higher score of WAS. The relationship between educated levels and WAS score was not significant. First admitted patients reported significantly lower score of WAS. Bipolar patients reported significantly higher score of WAS and alcohol related patients reported significantly lower score of WAS. Conclusion: The 1st admitted patients, patients with alcohol problem and educated patients were had less satisfied with psychiatric closed ward. Thus, by considering these factors, more specified therapeutic approach and plan should be conducted.