제조합 대장균에서 과발현된 Citrobacter freundii KCTC2006 유래의 β-Tyrosinase를 이용한 3,4-Dihydroxyphenyl-L-alanine의 생산

이승구,노현수,홍승표,이규종,왕지원,태동년,엄기남,방상구,김영준,성문희 한국미생물생명공학회 ( 구 한국산업미생물학회 ) 1996 한국미생물·생명공학회지 Vol.24 No.1

재조합 대장균에서 대량발현 시킨 Citrobacter freundii KCTC 2006 유래이 효소 β-tyrosinase를 이용하여 pyrocatechol, sodium pyruvate, ammonium acetate로부터 3,4-dihydroxy phenyl-L-alanine을 생산하기 위한 연구를 수행하였다. 이 효소반응에 적합한 온도 및 pH 조건은 각각 18℃와 8.5로 결정되었고, 반응액 중의 ammonium acetate와 sodium pyruvate의 농도는 각각 300 mM, 50 mM 이상으로 조절하는 것이 적합하였다. Pyrocatechol의 경우는 20 mM에서 가장 높은 반응성을 나타냈으나, 기질을 반복적으로 첨가하며 장시간 동안 효소반응을 수행하는 경우에는 pyrocatechol의 고갈을 피하기 위하여, 20 mM에서 50 mM 사이로 조절하였다. 한편, 반응액 중에 ethanol을 10% 첨가한 경우에는 반응속도가약 20% 증가하였다. 이상과 같은 효소반응특성에 기초하여 조제한 기질용액에 β-tyrosinase를 1 unit/㎖ 농도로 가하고, pyrocatechol과 pyruvate가 고갈되지 않도록 간헐적으로 첨가하면서 효소반응을 수행한 결과, 24시간 만에 85.2%의 수율로 31.6g/ℓ의 3,4-dihydroxyphenyl-L-alanine를 생산할 수 있었다. By using the β-tyrosinase of Citrobacter freundii KCTC2006, which was cloned and overexpressed in Escherichia coli, 3,4-dihydroxy phenyl-L-alanine (L-DOPA) was synthesized efficiently from pyrocatechol, sodium pyruvate, and ammonium acetate. Optimal temperature and pH for the reaction were determined to be about 18℃ and 8.5, respectively. The effects of substrate concentrations were also examined at different concentrations of ammonium acetate, sodium pyruvate, and pyrocatechol. Ammonium acetate and sodium pyruvate increased the reaction rate until the concentrations reached to 300 mM and 50 mM, respectively. Although pyrocatechol showed the optimal concentration at 20 mM, it was controlled between 20 mM and 50 mM to avoid the depletion of substrate during the enzymatic synthesis. Based on above results, a reaction medium for the production of L-DOPA was prepared and incubated with 1 unit/㎖ of β-tyrosinase. Pyrocatechol and sodium pyruvate was added to the reaction solution intermittently to avoid the substrate depletion during the enzymatic reaction. After 24 hour of reaction, 31.6 g/ℓ L-DOPA was accumulated in the reaction solution as soluble and precipitated ones and the conversion yield was about 85.2%.

상수도 매설구간 공동의심부 3D-GPR 지반탐사 사례

문태남 ( Moon Tae Nam ),구문수 ( Gu Moon Su ),신창건 ( Shin Chang-gun ) 한국물환경학회 2020 한국물환경학회·대한상하수도학회 공동 춘계학술발표회 Vol.2020 No.-

최근 상수관로의 미세 누수 등으로 인한 공동부 생성 및 확대로 지반침하가 빈번히 발생하고 있다. 본 사례는 시설물의 안전관리 중 발견된 도로부(상수관 매설지역)에 요철현상이 관찰됨에 따라, 공동 여부를 파악하기 위해 3D-GPR 지반탐사를 실시하였다. 차량탑재형 GPR탐사기기를 사용하여 도로부에 총 4회(왕복2차선 도로) 탐사를 실시하였으며, 탐사결과 공동추정 신호 3개소를 확인하였다. 발견된 공동추정 신호 3개소 모두 가스관 주변지반범위(지하안전법 시행규칙 별표 3의2에 따름) 내 위치하고 있었고, 이는 가스관 매설 후 되메움 불량 및 강우 시 유선 등의 발달로 토양 세립자가 유실되면서 공동이 발달한 것으로 추정된다. 또한, 도로 상암석 등을 적재한 중차량 통행이 빈번한 점, 공동추정신호가 하나의 측선(측선번호 3)에 집중되어 탐사된 점을 고려할 때, 적재 중차량 통행이 공동 발달에 추가적으로 영향을 미친 것으로 판단되었다. 해당 의심추정 신호는 상수도 주변지반 범위 밖으로 2차 조사시 발생할 수 있는 위험(도로부 천공) 등을 고려할 때, 해당 지중시설물 관리주체 등 관련 담당자와 협의를 통해 2차 조사 수행 기관결정 등이 필요하며, 2차 조사(천공 및 내시경 작업)를 통한 공동 확인 후, 공동 크기 및 보수 물량 등을 결정하여 보수여부 결정이 필요할 것으로 판단된다.

상수도 매설구간 공동의심부 3D-GPR 지반탐사 사례

문태남 ( Moon Tae Nam ),구문수 ( Gu Moon Su ),신창건 ( Shin Chang-gun ) 한국물환경학회 2020 한국물환경학회·대한상하수도학회 공동 춘계학술발표회 Vol.2020 No.-

최근 상수관로의 미세 누수 등으로 인한 공동부 생성 및 확대로 지반침하가 빈번히 발생하고 있다. 본 사례는 시설물의 안전관리 중 발견된 도로부(상수관 매설지역)에 요철현상이 관찰됨에 따라, 공동 여부를 파악하기 위해 3D-GPR 지반탐사를 실시하였다. 차량탑재형 GPR탐사기기를 사용하여 도로부에 총 4회(왕복2차선 도로) 탐사를 실시하였으며, 탐사결과 공동추정 신호 3개소를 확인하였다. 발견된 공동추정 신호 3개소 모두 가스관 주변지반범위(지하안전법 시행규칙 별표 3의2에 따름) 내 위치하고 있었고, 이는 가스관 매설 후 되메움 불량 및 강우 시 유선 등의 발달로 토양 세립자가 유실되면서 공동이 발달한 것으로 추정된다. 또한, 도로 상암석 등을 적재한 중차량 통행이 빈번한 점, 공동추정신호가 하나의 측선(측선번호 3)에 집중되어 탐사된 점을 고려할 때, 적재 중차량 통행이 공동 발달에 추가적으로 영향을 미친 것으로 판단되었다. 해당 의심추정 신호는 상수도 주변지반 범위 밖으로 2차 조사시 발생할 수 있는 위험(도로부 천공) 등을 고려할 때, 해당 지중시설물 관리주체 등 관련 담당자와 협의를 통해 2차 조사 수행 기관결정 등이 필요하며, 2차 조사(천공 및 내시경 작업)를 통한 공동 확인 후, 공동 크기 및 보수 물량 등을 결정하여 보수여부 결정이 필요할 것으로 판단된다.

180˚ 곡관덕트에 연결된 직관덕트에서 층류정상 유동의 PIV계측에 의한 유동해석

박길문(Gil-moon Park),이행남(Haeng-nam Lee),손현철(Hyun-chull Sohn),이종구(Jong-gu Lee),이홍구(Hong-gu Lee),이덕구(Duct-gu Lee),윤완중(Wan-jung Yun) 대한기계학회 2002 대한기계학회 춘추학술대회 Vol.2002 No.5

The velocity vectors and kinetic energy of laminar steady flows in the exit region connected to the square-sectional 1800 curved duct investigated experimental by PIV measurements.<br/> The experimental study for air flows was conducted to measure velocity vectors and kinetic energy distributions by using the Particle Image Velocimetry (PIV) system with the data acquisition of cactus'2000 software.<br/> The results obtained from experimental studies arc summarized as follows :<br/> (1) The Velocity vector distributions in the entrance region was influenced by centrifugal force of the curved duct as the flow develops to downstream in the exit duct<br/> (2) The maximum kinetic energy of laminar steady flow was gradually increased as the Reynolds number (Re) increased.<br/> (3) The entrance length is correlated as ??=0.026 in developing laminar steady flows. The entrance length is a function of hydraulic diameter (D_h)and Reynolds number(Re).

항장요법에 의한 白毛藤의 즉각형 과민반응 억제효과

문구,문석재,원진희,김태균,배남규,이종덕,임미양 대한동의병리학회 2001 동의생리병리학회지 Vol.15 No.5

The Solanum Iyratum Thunb. (Solanaceae) has been used against allergic disease for generations, and it still occupies an important place in traditional oriental medicine. In this study, the author investigated the effect of an aqueous extract of Solanum Iyratum on immediate-type hypersensitivity by anal administration. Anal administration of Solanum Iyratum showed a marked inhibition rate in systemic hypersensitivity with a dose of 0.1, 1 g/kg 1 hr before intraperitoneal injection of compound 48/80. Anal administration of Solanum Iyratum significantly reduced plasma histamine contents induced by compound 48/80. Anal administration of Solarium Iyratum (0.1 g/kg) also inhibited to 61.2%(P〈0.01) local allergic reaction activated by anti-dinitrophenyl (DNP) lgE significantly. In addition, Solanum Iyratum dose-dependency inhibited the histamine release from the peritoneal mast cells by compound 48/80 or anti-DNP IgE, When Solanum Iyratum was added, the level of cAMP in peritoneal mast cells transiently and significantly increased compared with that of basal cells. These results provide evidence that anal therapy of Solanum Iyratum may be beneficial in the treatment of systemic and local immediate-type hypersensitivity by inhibition of histamine release from mast cells in vivo and in vitro.

정상 혹은 감소된 혈장 ACTH 를 보이면서 IPSS에 의해 진단된 쿠싱증후군 2예

문태건,남대근,문재현,황지윤,김지홍,안진광,손석만,김인주,김용기,김학진,조용구 대한내과학회 2002 대한내과학회지 Vol.62 No.5

Cushing's syndrome results from prolonged exposure to high levels of glucocorticoid hormones. ACTH-dependent Cushing's syndrome accounts for about 85% of endogenous causes. Autonomous pituitary ACTH secretion, called Cushing's disease, is responsible for 80% of these causes, whereas ectopic ACTH secretion is responsible for 20% of them. Appropriate therapy of patients with Cushing's syndrome depends on accurate diagnosis and classification of the disorder. In addition to the history and clinical evaluation, the laboratory evaluation of a patient with Cushingoid appearance is necessary to establish the diagnosis and determine the cause of hypercortisolism. A major problem in the differential diagnosis of ACTH-dependent Cushing's syndrome is distinguishing Cushing's disease from the ectopic ACTH syndrome. Both entities can have similar clinical and laboratory features. In addition, both pituitary microadenoma and ectopic ACTH-secreting tumors may be radiologically occult. Bilateral inferior petrosal sinus and peripheral vein catheterization with simultaneous collection of samples for measurement of ACTH is one of the most specific tests available to localize the source of ACTH production. We report two cases of Cushing's syndrome diagnosed by inferior petrosal sinus sampling associated with normal or low ACTH levels on routine basal hormone assay. -

Electrochemical Performance of Li-Ion Batteries Containing Biphenyl, Vinyl Ethylene Carbonate in Liquid Electrolyte

Nam, Tae-Heum,Shim, Eun-Gi,Kim, Jung-Gu,Kim, Hyun-Soo,Moon, Seong-In The Electrochemical Society 2007 Journal of the Electrochemical Society Vol.154 No.10

A New White Wheat Cultivar, "Younbaek" for Noodle Making

Moon Seok Kang,Jong Jin Hwang,Choon Ki Lee,Jung Hyun Nam,Hyong Ho Park,Chul Soo Park,Jae Hwan Seo,Yang Gil Kim,Mi Ja Lee,Chong Chul Park,Jae Seong Choi,Seong Gu Lee,Sung Ju Kang 한국육종학회 2006 한국육종학회지 Vol.38 No.2

“Younbaek”, a new noodle making wheat cultivar, was developed from the cross between “Keumkang” with white grain color and “Tapdongmil” by the Honam Agricultural Research Institute(HARI), National Institute of Crop Science (NICS), RDA, Korea in 2005. Amon

Purification and Identification of Antioxidant Compounds from Dolichos lablab L. Seeds

Nam Woo Kwon(권남우),Jae Yeon Kim(김재연),Yong Beom Cho(조용범),Bang Yeon Hwang(황방연),Jun Gu Kim(김준구),Sun Hee Woo(우선희),Moon Soon Lee(이문순) 한국약용작물학회 2019 한국약용작물학회지 Vol.27 No.6

Background: This study aimed to identify antioxidant compounds from the seeds of Dolichos lablab L. by bioassay-guided isolation and recrystallization. Methods and Results: The water layer of D. lablab L. seed extract inhibits intracellular reactive oxygen species (ROS) expressing the 2’,7’-dichlorofluorescein diacetate (DCF-DA), Cu/Zn superoxide dismutase (SOD) and catalase genes, as determined by quantitative real-time PCR (qRTPCR). Two compounds were purified from the water layer of the seeds of D. lablab L. using column chromatography and prep-high performance liquid chromatography (HPLC). Using nuclear magnetic resonance (NMR) and electrospray Ionization mass spectrometry (ESI-MS), their chemical structures were identified as 5-[(2-acetyl-2,3-dihydro-1H-indazol-1-yl)carbonyl]-4,5-dihydro-3H–furan-2-one (C<SUB>14</SUB>H1<SUB>4</SUB>N₂O₄) and stachyose. Conclusions: Two active antioxidant compounds were purified from the seed extract of D. lablab L. seed extract and the structures of these compounds were identified as C<SUB>14</SUB>H<SUB>14</SUB>O₄N₂ and stachyose.

Polymorphism and Phylogenetic Analyses in Rices Cultivated in Korea by RAPDs and RFLPs

김남수,신영범,은무영,조용구,윤경민,심교문,조윤상 한국유전학회 1996 Genes & Genomics Vol.18 No.3

Genetic variations in rice germplasm were investigated using RAPD and RFLP analysis. Seventy varieties of rice, including 31 Korean varieties, 18 Korean landraces, and 21 foreign accessions, were analysed. Seventy-three out of 273 RAPD and RFLP fragments were polymorphic among the seventy vices. The variation among the Korean landraces was lower than adapted modern cultivars, which was contrast to other reports. We detected several RFLP fragments which were present only among the Korean cultivars and foreign accessions but absent in the landraces. Therefore, these fragments were regarded as the DNA fragments which were introduced from foreign germplasm during the cultivar development by crosses between Korean varieties and indica typed foreign rice varieties. On the contrast with these fragments, one fragment was present among the landraces and foreigners but not in the Korean cultivars. This fragment was considered to be the DNA fragment that was screened out in the courses of cultivar developing programs. So, these RFLP fragments could be useful in rice breeding program if they are related to agronomically important traits. The genetic variation among the indica vices was higher than that of japonica rices and the japonica rices were differentiated from the indica ones at the similarity level of 0.75.