두부 손상이 골절 칭 촉진 시동물 모델 및 유전자 발현 분석

김경희,김학선,문성환,김향,권언해,이환모,박희완,한수봉,문은수 대한정형외과연구학회 2003 대한정형외과연구학회지 Vol.6 No.2

통풍성 관절염의 임상적 고찰

윤채중,정승문,김영학,김동규,허광식,김태원,배학연,정종훈,이승일,김평남 朝鮮大學校 附設 醫學硏究所 1997 The Medical Journal of Chosun University Vol.22 No.2

통풍은 Purine 대사의 이상으로 발생하는 질환으로, 고뇨산혈증인 사람의 전부가 통풍으로 발현되지 않고 증상의 출현 양상이 다양하여 진단과 치료에 주의가 필요하며, 조기에 적절한 조치를 한다면 충분히 조절이 가능한 질환이다. 본대학 내과학교실에서는 통풍으로 치료한 32명의 환자에서 임상양상, 병력과 검사소견을 분석하여 다음과 같음 결과를 얻었다. 1. 32명 모두 남자이며, 최초 발병 시기는 24세에서 72세로 평균 43.8±11.9세이었으며, 30대에서 40대까지가 19명(59.4%)으로 대부분을 차지하였다. 내원 당시 나이는 27세에서 75세까지로 평균 52.3±10.4세였으며, 내원시 까지 평균 유병기간은 8.5±6.8년으로 나타났다. 2. 동반 질환으로는 고지혈증 12례, 신장질환 10례, 고혈압 12례, 비만 8례, 당뇨 2례 등이었다. 3. 이환된 관절은 단관절 침범이 19례(59.4%), 다관절 침범이 13례 이었으며, 최초 이한된 관절은 족무지 중족골지골 관절로 19례(59.4%)로 가장 많았고, 통풍 결절은 20례(62.5%)에서 관찰되었으며, 유병기간이 10년 이상된 12례중 11례 (91.7%)에서 결절이 관찰되었다. 4. 평균 혈중 요산치는 9.17±1.75 ㎎/dl이었으며, 8.0 ㎎/dl에서 9.9 ㎎/dl 사이가 19명으로 전체의 59.4%를 차지하였다. Objective: The gout is a heterogeneous group of diseases resulting from tissue deposition of monosodium urate or uric acid crystals from extracellular fluids supersaturated with respect to this end product of human purine metabolism. The clinical manifestations are such as hyperuricemia, gouty arthritis, gouty nephropathy, uric acid nephrolithiasis. We analyze of clinical manifestations and associated factors in gout. Method: We have reviewed the medical records, radiologic findings and clinical results of thirty-two patients admitted at our department from April 1996 to July 1997. Result: 1) All patients were male. The mean age at initial attack was 43.8 years old, ranging from 24 to 72 years old. 2) The mean level of serum uric acid was 9.17mg/dl on admission. 3) The first metatarsopharyngeal joint was involved in 19 cases (59.4%). Tophus was observed in 20 cases (62.5%). 4) Hyperuricemia was associated with hypertension, obesity, nephrolithiasis and hyperlipidemia.

Single-Chip 마이크로 콘트롤러를 이용한 이상 전류 검출기

김경렬,나채동,문학룡,유두영 崇實大學校 生産技術硏究所 1993 論文集 Vol.23 No.-

The development of the measurement technology has occupied an important position of not only the electrical, electronic but also scientific technology sphere. In the past, the abnormal current detector was used by the analog method, had many the trouble and the inconvenience of measurement, dissatisfied the accuracy and had difficulty in storing, preserving the data. In this paper, these are improved by using single-chip microcontroller which detects the abnormal current and saves in back-up RAM after digitizing. For the efficient management, processing of the memory, it was used the index method. The data in the memory can be transmitted to the personal computer by the serial communication, and also be independently transmitted by the parallel communication to the personal computer which is interfaced with. The software to control system components are structured in assembly language and C language, in order to process and analyze the software is used in C language.

액체막법에 의한 중금속이온의 분리 및 회수

曺文換,丁學鎭,이상인,金振浩,金始中 江原大學校 附設 環境硏究所 1994 環境硏究 Vol.11 No.-

거대고리 리간드는 금속이온과 선택적으로 결합하는데, 이를 이용하여 에멀젼 액체막을 통한 금속이온의 이동에 관하여 연구를 수행하였다. 금속이온은 유기층에 있는 운반체에 의하여 source phase에서 receiving phase으로 이동한다. 운반체로는 거대고리 리간드인 DBN_(3)O_(3)를 사용하였다. 여기에서 에멀젼 액체막을 통한 금속이온의 이동에 관한 요인과 어떤 금속이온의 선택적 분리에 관하여 검토하였다. 금속이온과 거대고리 리간드 그리고 금속이온과 receiving phase내에 있는 음이온에 대한 안정도 상수를 금속이온의 선택적 이동에 대한 척도로 조사하였다. 납이온이 혼합 용액에서 다른 금속이온보다 높은 이동속도를 나타내었다. Receiving phase내의 음이온이 금속이온의 이동에 중요한 역할을 한다. Receiving phase내에 있는 NO_(3)^(-)을 S_(2)O_(3)^(2-) 대치하면 이동량이 증가함을 보였는데 이는 Pb^(2-)-S_(2)O_(3)^(2-) 상호작용이 Pb^(2+)-NO_(3)^(-) 상호작용보다 크기 때문이다. Macrocyclic ligand has been know to selectively bind with metal ions so that ability applied for the transport of metal ions across the emulsion liquid membrane in this study. The metal ions are transproted from the source phase to the receiving phase by the carrier of the organic phase. Several factors involved in the transport of metal ions acrose the emulsion membrane we reported here and these factors proveided the informations for the selective seperation of some metal ion. Stability constants for cation-macrocyclic ligand and metal ion-anion receiving phase interaction are examined as parameters for the prediction of metal ion transport selectivities Pb^(2+) was transported higher rates than the other metal ions in the mixture solution. The interaction of metal ion to anion in receiving phase is important. S_(2)O_(3)^(2-) in replacement of NO_(3)^(-) in the receiving phase enhances the transport of pb^(2-) since Pb^(2-)-S_(2)O_(3)^(2-) interaction is greater than Pb^(2+)-NO_(3)^(+) interaction.

대형 재해관리의 문제점과 개선방안

김학수,고재문 한국응급구조학회 2003 한국응급구조학회지 Vol.7 No.1

If see disaster administration system of our country, start in terms of is coping by countermeasure after four immediately after disaster occurrence many problems blessing with a sons by tribe and so on of link nature between formation, disaster administration complete charge utensil's absence, disaster charge manpower and budget be indicated and join. If examine improvement way accordingly, is as following; Necessity of synthetic disaster administration system, Fire fighting formation's independence guarantee. Integration of fire fighting connection similarity business. Disaster administration's permanent establishment complete charge utensil's necessity, disaster administration midautumn complete charge utensil at a metropolitan autonomous fire fighting system reorganization. Role division of labor between center and local government, Disaster administration professional human strength positivity, Disaster administration information system construction practical use, Equipment and improvement of budget state, Education public information for safety culture consciousness fixing, Internationalization of fire fighting business, globalization propulsion, Structure, member of rescue confrontation system and efficiency. Fire fighting environment is changing greatly, and fire fighting must become center to correspond to do confrontation that do one thing troble when produce disaster.

HBx형질전환 생쥐에서 발생한 간세포암종에서 H-ras 및 c-myc의 발현에 관한 연구

문형배,소병준,김학철,윤기중,한원철,조향정,유대열,정영진 圓光大學校 醫科學硏究所 2002 圓光醫科學 Vol.17 No.2

<연구목적> HBx형질전환 생쥐에서 발생한 간세포암종의 발암과정에 종양유전자(H-rgs, c-myc)의 발현 정도를 조사하고자 하였다. <연구방법> 정상생쥐 12마리(4-18개월) 및 HBx 형질전환 생쥐 44마리(4-18개월)를 대상으로 포르말린에 고정하고 파라핀에 포매한 간 조직을 이용하여 면역조직화학적염색을 실시하였다. 실험군은 정상 부위, 이형성 부위 및 종양 부위로 구분하였으며, 종양 부위는 소결절성병변 부위와 간세 포암종 부위로 구분하였고, 이형성병변 부위는 이형성병변만 발견되는 부위, 소결절성병변과 동반된 이형성병변 부위 및 간세포암종과 동반된 이형성병변 부위로 구분하였다. <연구결과> H-rgs의 발현은 정상 간조직에 비하여 이형성병변 부위(P<0.05) 및 종양 부위(P<0.01)에서 증가하였으며, 소결절성병변 부위과 간세포암종 부위 사이에서는 간세포암종 부위에서 증가된 경향이었으나 통계학적으로 유의한 차이가 없었으며, 각 이형성병변 부위 사이에서도 유의한 차이는 없었다. c-myc의 발현은 정상 간조직 및 이형성병변 부위에 비해 종양 부위에서 증가하였으며(P<0.001), 소결절성병변 부위와 간세포암종 부위에서는 비슷하였고, 각 이형성병변부위 사이에서도 비슷하였다. <결론> HBx형질전환 생쥐에서 발생하는 간세포암종의 발생에 H-rgs는 이형성 변화를 일으키는 시기에 관여하며, c-myc은 이형성병변에서 암으로 이행하는 시기에 관여할 것으로 생각한다. Background: This experiment was designed for the expression of H-ras and c-myc in hepatocarcinogenesis of the HBx transgenic mice. Methods: Immunohistochemical stains in the paraffin embedded tissue of the liver were used for the detection of H-ras and c-myc in the 12 normal mice and 44 HBx transgenic mice of the 4-18 month old. Results: Expression of the H-ras was significantly increased in the dysplastic area (P<0.05) and tumor area (P<0.01) than in the normal liver. But there were no differences of H-ras expression between areas of microscopically identified hepatocellular carcinoma (MI-HCC) and grossly identified hepatocellular carcinoma (GI-HCC) and dysplastic areas among the only dysplastic areas, dysplastic areas with MI-HCC and GI-HCC. Expression of the c-myc was significantly increased in the tumor area (P<0.001) than in the normal liver and dysplastic area. But there were no differences of c-myc expression between areas of MI-HCC and GI-HCC, and dysplastic areas among only dysplastic areas, dysplastic areas with MI-HCC and GI-HCC. Conclusions: Our study suggests that H-ras is related to the dysplastic change and c-myc is related to the neoplastic change in the hepatocarcinogenesis of the HBx transgenic mice.

경골 근위부 자가 망상골을 이용한 치조열 골 이식술

김학균,김은석,문재필,정창우 충남대학교 의과대학 지역사회의학연구소 1998 충남의대잡지 Vol.25 No.2

Two cases of alveolar bone graft with autogenous cancellous bone from proximal tibial metaphysis was done. Satisfactory amount and quality of autogenous cancellous bone was obtained. Considering its overall lack of morbidity, the proximal tibial metaphysis is a useful donor site for small to moderate bone defect in oral & maxillofacial region.

핑거형 신축이음장치의 연결부에 대한 안전성 검토

김운학,유영화,박문식 안성산업대학교 2002 論文集 Vol.34 No.-

Several considerations on structural safety of connection part in Finger-Type expansion joint system were presented in this paper. Special considerations were focused on the compressive strength of sealant concrete, the stress of anchor bolt through introducing prestressing force and he reasonability on the magnitude of prestressing force. Tow-and three-dimensional finite element analyses using MIDAS have been performed to check the structural safety of the expansion joint system. The analysis results showed that the excessive compressive stress acting on concrete can be dispersed or reduced only when over 40% of maximum tensile stress acting on bolt obtained by hand calculation is added to the prestressing force. And the design compressive strength of sealant concrete over 400 kgf/㎠ is reasonable, but more attention should be paid to quality control and construction management even though he design value of 500 kgf/㎠ in original design can improve a structural safety.

계면활성제 용액중에서 diazo coupling 반응을 이용한 procaine의 흡광도 정량

김영학,안문규 慶星大學校 1999 論文集 Vol.20 No.2

The use of a micellar solution as a means for improving the colorimetric determination of procaine by diazotization and coupling method is studied. SDS, Triton X-100 and N-CPC, together with the diazotizable substances procaine and coupling agents 1-naphythylamine, NED and H-Acid are used as model compounds. Procaine-azo dyes in surfactants solution, the coupling rate increase greatly when pH is below 3.5, allowing the possibility to obtain the protonated dye directly and quickly, thus avoiding the last pH adjustment of the conventional procedure for the determination of procaine. The calibration curves were linear up to 10? ㎍/mL ~ 100 ㎍/mL for procaine. The relative standard deviation for the analysis of five replicates of a solution containing 50 ㎍/mL of procaine was 1.7%.

백서에서 신 허혈성 손상에 미치는 칼슘 길항제의 효과

문철웅,정종훈,박천국,이승일,배학연,장경식,김만우,정춘해,홍순표,이병래,김호중 朝鮮大學校 附設 醫學硏究所 1993 The Medical Journal of Chosun University Vol.18 No.1

Renal ischemia is one of the most common causes of acute renal failure. Four factors related to the pathogenesis of acute renal failure are vasoconstriction, decreased glomerular filtration rate, tubular back leak of filtrate, and intratubular obstruction. The cellular response to renal ischemic insults include decreased content of adenosine trihosphate, lipid peroxidation induced membrane degradation, alteration in cellular pH, and calcium or phospholipase induced mitochondrial dysfunction. Much attention has been given to the role of increased cellular calcium as a pathogenetic contributor to cell injury during ischemia. Author studied the protective effects of calcium antagonists on cellular injury during renal ischemia in rat. To investigate the protective role of these agents, author measured the amount of malondialdehyde(MDA) and the enzyme activities of free radical scarvengers-superoxide dismutase(SOD), catalase and glutathione peroxidase from non-pretreated group and calcium antagonists pretreated groups in control, ischemia and reflow subgroups. The results were summerized as follows: 1) The amount of MDA in non-pretreated group was higher in the reflow compared with the control(<p<0.01). But, in all pretreated groups, there was no statistically difference in the amount of MDA. 2) The SOD activity in non-pretreated group was lower in both the ischemia and the reflow compared with the control (P<0.05). But, in both verapamil and trifluoperazine-pretreated groups, there was no statistically difference in the SOD activity. 3) Both catalase and glutathione peroxidase activities in non-pretreated group were lower in both the ischemia and the reflow compared with the control (P<0.05). But in all pretreated groups, there was no statically difference in both catalase and glutathione peroxidase activities. These results suggest that free radical mediated ischemic injury by renal artery clamp in rat can be protected by intraperitoneal pretreatment with calcium antagonists. As trifluoperazine has a protective effect in renal ischemia, the calcium activated calmodulin dependent enzyme may play a role in renal ischemic injury.