Lidocaine, Thrombin, Epinephrine 의 항균효과

김진우,이동건,전혜선,김승준,김석찬,안중현,김치홍,권순석,김영균,김관형,문화식,신완식,송정섭,박성학 대한감염학회 2005 감염과 화학요법 Vol.37 No.6

목적 : 기관지내시경 검사에서 흔히 사용되는 국소마취제인 lidocaine과 내시경시 지혈목적으로 사용되는 thrombin과 epinephrine이 각종 균주에 미치는 항균효과에 대해 알아보고자 하였다. 재료 및 방법 : 균주는 가톨릭대학교 성모병원에서 2004년 3월부터 2004년 9월까지 임상검체 에서 동정된 S. aureus, S. pneumoniae, K. pneumoniae, P. aeruginosa 각각 42, 42, 42, 43주를 대상으로 하였다. Lidocaine, thrombin, epinephrine 감수성 검사는 National Committee for Clinical Laboratory Standards (NCCLS, 2002)의 기준에 따랐다. 결과 : Lidocaine은 S. aureus, S. pneumoniae, P. aeruginosa에서 MIC_(50), MIC_(90) 모두 20,000 ㎍/mL 이었다. K. pneumoniae는 각각 10,000 ㎍/mL이었다. Thrombin은 S. aureus와 P. aeruginosa에서 MIC50 500 lU/mL 과 MIC_(90) 500 IU/mL 이상이었고, K. pneumoniae에서는 MIC_(50)과 MIC_(90)이 모두 500 lU/mL이상이었으나 S. pneumoniae에서는 MIC_(50)과 MIC_(90)은 125 IU/mL이었다. Epinephrine은 K. pneumoniae, S. pneumoniae에 대한 MIC_(50), MIC_(90)가 모두 >500 ㎍/mL이었고, S. aureus와 P. aeruginosa에 대한 MIC_(50), MIC_(90)가 모두 500 ㎍/mL이었다. 결론 : 기관지 내시경 검사에서 흔히 쓰이는 lidocaine, thrombin, epinephrine 등의 약제들이 호흡기 질환의 흔한 병원균인 S. aureus, S. pneumoniae, K. pneumoniae, P. aeruginosa 균주들에 대해 항균 효과가 있을 수 있어 세균배양검사에 영향을 미칠 수 있겠다. Background : We performed this stody to find out about antimicrobial effect of lidocaine which is commonly used local anesthetic, and thrombin and epinephrine used for hemostasis during bronchoscopic procedures. Materials and Methods : The microorganisms that were cultured from specimens obtained during bronchoscopy were Staphylococcus aureus (n=42), Streptococcus pneumoniae (n=42), Klebsiella pneumoniae (n=42), and Pseudomonas aeruginosa (n=43) collected from St. Mary's Hospital, from March to Sep 2004 were used for susceptibity testing. Susceptibility to lidocaine, thrombin, and epinephrine were tested according to the National Committee for Clinical Laboratory Standards. Result : MIC_(50) and MIC_(90) of lidocaine for S. aureus, S. pneumoniae, P. aeruginosa were all 20,000 ㎍/mL and that for K. pneumoniae were 10,000 ㎍/mL. MIC_(50) and MIC_(90) of thrombin for both S. aureus and P. aeruginosa was 500 IU/mL and above 500 IU/mL, respectively; that for K. pneumoniae were all above 500 IU/mL and for S. pneumoniae they were 125 IU/mL, MIC_(50) and MIC_(90) of epinephrine for K. pneumoniae and S. pneumoniae were above 500 ㎍/mL; that for S. aureus and P. aeruginosa were 500 ㎍/mL. Conclusion : We observed possible antimicrobial effect of lidocaine, thrombin, and epinephrine in vitro against pathogens such as S. aureus, S. pneumoniae, K. pneumoniae, P. aeruginosa, which are common respiratory microorganisms. The use of these agants could affect the result of bacterial culture.

혈액암환자에서 조혈모세포 이식 후 따르는 헤르페스 바이러스 감영 양상

이호섭,탁희상,신성훈,김양수,남성진,김혜수,박진희,정수현,김성빈,김예나 고신대학교 의과대학 2010 고신대학교 의과대학 학술지 Vol.25 No.1

배경 : 헤르페스바이러스과에는 단순포진 바이러스(HERPES SIMPLEX VIRUS, HSV), 수두대상포진 바이러스 (VARICELLA-ZOSTER VIRUS, VZV), 엡스타인-바 바이러스 (EPSTEIN-BARR VIRUS, EBV), 그리고 거대세포 바이러스 (CYTOMEGALOVIRUS, CMV) 등이 있다. 헤르페스바이러스 감염은 혈액암환자에서 조혈모세포 이식 환자에서 전파 (dissemination), 이차감염, 세균 중복 감염의 심각한 합병증을 일으킬 수 있다. 하지만 항암약물치료를 받는 림파종 환자에서 항 바이러스 약제의 사용 용량과 기간에 대한 concensus는 없다. 헤르페스바이러스 감염에 대한 발생율과 위험인자를 후향성으로 분석하였다. 방법 : 고신대학교 복음 병원에서 1995년 6월부터 2009 년 2월 사이에 새롭게 진단되어 조혈모세포 이식을 받은 전체 58명의 환자들이 후향적으로 현 연구에 등록했다. 헤르페스 바이러스 감염은 임상적 진단, 혈청학적 검사 또는 병리학적 진단에 기초하여 확진한다. 환자들의 특성은 다음과 같다: 평균 연령은 44세 (범위 19-62세)였고 남녀비는 30:28이었다. 등록된 질환은 백혈병 (n=9, 15.5%), 림프종 (n=30, 51.7%), 다발성 골수종 (n=12, 20.7%), 재생불량성 빈혈 (n=6, 10.3%) 그리고 골수이형성증후군 (n=1, 1.7%)이 포함되었다. 결과는 chi-squre test나 independent samples T test를 사용하여 분석되었다. Multivariate analysises에 대해 logistic regression test를 사용하였다. 결과 : 15명의 환자들 (25.9%)에서 조혈모세포 이식후 헤르페스 바이러스 감염이 발생하였다. 조혈모세포 이식 5년간 누적 발생율은 53.9%였다. Univariate analyses에서 헤르페스 바이러스 감염에 대한 유의한 위험 인자는 없었다. 하지만 조혈모세포 이식후 생존 기간(40.18 ± 30.14 months vs 20.06 ± 26.67 months, p=0.018)은 헤르페스 바이러스 감염의 낮은 발병율과 유의한 관련성이 있었다. 이식 편대 숙주질환 (GVHD)의 존재 (37.5% in developed GVHD vs 0% in no GVHD, p=0.200), 동종조혈 모세포 이식에서 면역억제요법의 기간 (15.98 ± 14.02 months vs 6.78 ± 3.67 months, p = 0.374)은 헤르페스 바이러스 감염에 대한 위험인자가 아니었다. 결론 : 조혈모세포 이식 후 생존 기간은 어사이클로비어 예방을 받은 혈액암 환자에서 헤르페스 바이러스 감염의 낮은 발병율과 연관성이 있는 것으로 보인다. 저용량의 어사이클로비어 예방은 헤르페스 바이러스 감염의 고위험군인 조혈모세포 이식을 받은 혈액암 환자에 필요하리라 생각된다. Background : Herpesviridae family includes herpes simplex virus, varicella zoster virus, Epstein-Barr virus, and cytomegalovirus, etc. Herpesviridae viral infection (HVI) can lead to serious complications including dissemination, secondary infection, bacterial superinfection in patients with hematologic malignancy following hematopoietic stem cell transplantation (HSCT). But there was no consensus on the dose and duration of antiviral agents prophylaxis in patients undergoing chemotherapy. We retrospectively analyzed the incidence and the risk factors for HVI. Method : A total of 58 patients who newly diagnosed and received HSCT with prophylaxis of acyclovir at the Kosin University Gospel Hospital, Busan, Korea between June 1995 and February 2009 were enrolled retrospectively in the current study. HVI was confirmed based on clinical diagnosis, serologic test or pathologic diagnosis. The characteristics of the patients were as follows: the median age was 44 years (range 19-62 years) with a female-to-male ratio of 30:28. The enrolled diseases included leukemia (n=9, 15.5%), lymphoma (n=30, 51.7%), multiple myeloma (n=12, 20.7%), aplastic anemia (n=6, 10.3%) and myelodysplastic syndrome (n=1, 1.7%). The results were analyzed using a chi-square test and independent samples T test. For the multivariate analysis, we used logistic regression test. Results : Fifteen patients (25.9%) developed HVI after HSCT. The cumulative incidence of HVI was 53.8% at 5 years after HSCT. In univariate analysis, there was no significant risk factor for HVI. The presence of graft-versus-host disease (GVHD) (37.5% in developed GVHD vs. 0% in no GVHD, p=0.200), duration of immunosuppressive therapy (IST) in allo-SCT (15.98 ± 14.02 months vs. 6.78 ± 3.67 months, p = 0.374) were not risk factors for HVI. Conclusion : The incidence of HVI was similar to that in historical other studies. There was no risk factor associated with development of HVI. Most of the HVI occurred within the first 24 months after transplantation. So long term use of antiviral prophylaxis may be needed to prevention of HVI after HSCT.

토양처리용 천연물제초제 개발을 위한 생물검정법 개선

김재덕(Jae Deong Kim),황현진(Hyun Jin Hwang),서보람(Bo Ram Seo),최정섭(Jung Sup Choi),김진석(Jin Seog Kim) 韓國雜草學會 2011 Weed&Turfgrass Science Vol.31 No.3

본 연구는 토양처리용 천연물 제초제의 개발을 보다 신속하게 효율적으로 진행하기 위한 방안으로서 실용적인 입장에서 보다 개선된 검정법을 확립하기 위해 수행되었다. 검정하고자 하는 화합물의 량이 50㎎ 이상 충분할 경우, 잡초 4초종(피, 바랭이, 자귀풀, 어저귀)이 파종된 50㎠ 토양 표면적을 가진 폿트에서 온실검정을 함으로서 기존의 방법(토양 표면적이 350㎠인 폿트 이용)에 비해 약제 소요량을 78배 줄일 수 있었다. 이때 검정초기 처리농도는 10,000㎍ mL<;SUP>;-1<;/SUP>; 내외로 하는 것이 적당하였다. 한편 검정용 시료의 량이 10㎎ 이하로서 미량일 경우는 6 well plate를 이용하되 각 well에 밭토양을 담고 4가지 초종을 파종한 다음 실내에서 제초활성을 검정하는 방법이 추천될 수 있었다. 이는 기존의 방법(토양 표면적이 350㎠인 폿트 이용)에 비해 약제 소요량 및 처리용액 부피를 14배 가까이 줄일 수 있었고 실제에 가까운 데이터를 확보할 수 있어 보다 빠른 속도로 천연물 제초제를 개발하는데 도움이 될 것으로 판단되었다. This study was carried out to establish an improved bioassay system on the side of practicality, pre-emergence bioassay which is more effective in developing soil application natural herbicides. A miniaturized method which have a 50 cm² of soil surface area and was efficient by 7 times compared to the existing soil application assay (350 cm² of soil surface area) was established, in which four weed species (Echinochloa crus-galli, Digitaria sanguinalis, Aeschynomene indica, and Abutilon theophrasti) were planted and grown in greenhouse. This would be applicable when the amount of screening compound is much more than 50 mg. The initial application rate was desirable at 10,000 μg mL-1. On the other hand, the 6 well plate assay which has 4 weed species in each well containing upland soil and could be conducted in growth chamber, was established. This assay was resulted in minimizing in level of 1/14 test volume and 1/14 amounts of test compound to the conventional method that has been used for screening of synthetic compounds in KRICT, and applicable for the small amount of test compound (less than 10 mg). Therefore, the improved bioassays established in this study would be helpful for a rapid and efficient development of soil application natural herbicides.

忠北觀光開發과 새마을運動

金震燮 청주대학교 새마을연구소 1988 새마을硏究論文集 Vol.5 No.-

Chung-buk province is richly endowed with many natural tourism resources such as Mt. Wolak national park, Mt. Sokri, and Lake Danyang, to cite a few. Consequently, tourism looms large and the number of tourists continue to in-crease. When it comes to the development and planning of tourism, however, Chung-buk area lags far behind many other provinces. The objective of the paper is that of examining the common characteristics of tourism development, identifying the need for tourism planning and providing a model for developing tourism, The paper also delives into the possibility of developing tourism through Sea-maul Undong in this area. The aim is to make a critical evaluation of the social, economic and environmental issues that spawn the need for tourism development through a new community movement. It is suggested that home visit program, joint community development of resorts, and agricultural tourism will play a vital part in tourism development in this province. While the paper leaves much for further study, it can stimulate greater interest by students, academicians, businessmen, government policymakers and the very important tourist, himself, to demand deeper study of tourism development in this part of world.

상부소화관출혈에 있어서 내시경적 분말약제산포의 효과

김상균,조주영,송상훈,김진오,이준성,이문성,심찬섭 순천향의학연구소;Soonchunhyang Medical Research Institute 2000 Journal of Soonchunhyang Medical Science Vol.6 No.1

Background : Bleeding after endoscopic procedure of the stomach carries an increased risk of morbidity and mortality. In case of oozing hemorrhage, not in active hemorrhage, powdered sodium alginate may play a role in control of bleeding with convenience. Therefore, we evaluated the clinical effect and efficacy of sodium alginate powder in upper G-Ⅰ bleeding. Methods : Forty-five patients who had upper G-Ⅰ bleeding after endoscopic procedure were enrolled in this study. Twenty-five patients of them were managed by the application of powder preparation of Sodium alginate(ALTO). The others were observed without treatment. The next day, we performed endoscopy in all patients to ascertain the hemostasis. Results : Of 25 patients managed with Sodium alginate, only two patients(8%) had bleeding (hemostatic rate : 92%), On the other hand, of 20 patients who did not receive any treatment, 3 patient(15%) had bleeding (hemostatic rate : 85%). There was no statistical difference between the two groups(p>0.05). Using other endoscopic procedure, We successfully treated the patient who continued to bleed. Conclusion : We evaluated the effect of the spary of sodium alginate on oozing hemorrhage. Although there was no statistical significance in our study, it seems that the spray of sodium alginate may be a useful method for the prevention of bleeding after endoscopic procedure.

혼화재 및 폴리프로필렌섬유의 사용에 따른 콘크리트의 품질변화에 대한 기초적 연구

김정섭,손순채,조창호,최진석,김민국 朝鮮大學校 建設技術硏究所 2001 建設技術硏究 Vol.21 No.1

This study is to identify the effect of using admixture and polypropylene fiber on the quality of concrete. The results of the changes in the quality of concrete that has not yet hardened and concrete that has hardened is as follows: 1. Depending on the proportions of admixture, the greater the fly ash content and the lower slag and silica fume, the greater the slump. Admixture and polypropylene together contributed to increased slump. 2. The more fly ash, slag and silica fume added, the less the air. 3. After 360 days of age, greater fly ash content contributed to a lower compressive strength, while greater slag added to higher compressive strength. Silica fume shows a high compressive strength when 10% of silica fume was added on the 360th day of age. 4. Tensile Strength at 360 days of age, the less fly ash and the more slag, the higher the tensile strength. Silica fume at 360 days of age increase strength when the mixing proportion was 30%. 5. The more fly ash and the less slag and silica fume that were mixed, the greater the fire resistance. When added to and polypropylene fiber, the fire resistance of concrete of no effect. 6. The more fly ash and silica fume, the more carbonation shown and when there was only 10% slag present, the neutralization was also shown.

南部地方에서의 통일벼 生産性에 미치는 N.P.K. 肥料의 效果

金鎭雨,安元燮 진주산업대학교 1975 論文集 Vol.13 No.-

最近 育成된 統一벼 栽培法 改善의 一環으로 南部地方에 있어서 이品種에 대한 N.P.K.의 肥料反應을 규명하기 위하여 無肥區(N_0P_0K_0), 三要素施用區(N_14P_10K_11), P.K.施用區(N_0P_10K_11), N.K施用用區(N_14P_0K_11) 및 N.P.施用區(N_14P_10K_0)의 5가지 條件에서 試驗하였던바 얻어진 結果를 要約하면, 1. 出穗期, 成熟期, 穗長, 登熟率, 玄米千粒重, 精租千粒重 等은 NPK의 영향을 받지 않았으나, 稈長, 株當穗數, 穗當粒數, 精租收量, 玄未收量, 等은 有意性이 認定되었다. 2. 株當穗數에 대하여 N의 施用效果가 컸으나 P.K는 영향을 미치지 못하였다. 3. 穗當粒數에 대하여도 N의 效果가 크게 나타났으며 P效果가 K보다 더크게 作用한 것으로 보였다. 4. 種實收量에 대하여 N施用이 매우큰 영향을 미쳤으며 K나 P도 매우 깊은 관계가 있는 것으로 보아, N와 더불어 적정 수준의 P.K.施用量 문제를 조속히 해결해야 할 필요성을 가지게 되었다. In order to investigate the effects of N.P.K. on a new HYV. Tongil in the southern part of Korea. variety, Tonil, four lends of nitrogen fertilization omount i.e. 14. 10. 11. per 10a. of nitrogen was treated. The results are summarized as follows: 1. Among the abserved characters, flowering date, heading date, culm length, panicle length. ripend grain ratio. 2. 1000-grain weight of brown rice lodging index showed no significant difference among. 3. The different nitrogen levels No. of panicles per hill was increased under the heavy nitrogen fertilization, while No. of grains per penicle was decreased in this case. 4. An optimun nitrogen fertilization level for Tongil was estimated as or more and the basal fertilization for the development of effective tellering and of no of grains should be emphasized.

有·無機質肥料가 水稻의 生育 및 收量에 미치는 影響

金鎭雨,安元燮,崔周鎬 진주산업대학교 1984 論文集 Vol.22 No.-

本 試驗은 有機質 및 無機質肥料가 水稻의 生育 및 收量에 미치는 影響을 알기 爲하여 施行되었으며 그 結果를 要約하면 다음과 같다. 1) N 18㎏, K_2O_5 9㎏, K_2O 12㎏, 施用區가 收量이 가장 높았다. 2) 有機質肥料만 施用한 區보다 無機質肥料만 使用한 區보다 無機質肥料만 使用한 區가 收量이 增加되었고 有機質 및 無機質肥料를 混用한 區의 收量이 가장 높았다. 3) N 21㎏, P_2O_5 9㎏, K_2O 12㎏ 施肥區가 初期生育이 가장 旺盛하였으나 千粒重 및 登熟率이 낮은 것으로 나타나 相對的으로 收量이 떨어졌다. The experiment was carried out to find the effect of growth and yield of the paddy depend upon the source of application fertilizers like inorganic, organic and the mixture of organic and inorganic with varying levels. In this experiment, fertilizer(inorganic) applied at the rate of 18, 9 and 12㎏ per.10 a (N:P:k) showed the most productive seed yield of all treatments. In the yield experiment, plants which received inorganic fertilizer alone was hisher than that of organic fertilizer alone, and plants which received the mixture of organic and inorganic fertilizer was the highest of all. The initial growth of the plants which received 21­9­12㎏/10 a (N­P­k) was the highest As results of that 1000 grains weight(g) and filled grain(%) was dereased, the yield was decreased.

UV-TiO₂광촉매 반응기를 이용한 미생물의 살균효과

김시욱,이정섭,정해광,박열,윤성명,유진철,이범규,이인화,박진열 조선대학교 환경공해연구소 1998 環境公害硏究 Vol.14 No.-

UV-TiO_(2) 반응기를 이용하여 Escherichia coli와 Aspergillus oryzae var. oryzae의 살균효과를 측정하였다. 254 ㎚에서 최대 14 watt의 자외선 방출량을 내는 램프를 원형 Pyrex유리관 중앙에 설치하였고 TiO_(2)는 석영관에 박막증착(Thin Film Coating)된 형태와 슬러리 형태로 나누어 회분식으로 살균정도를 측정하였다. E. coli에 대한 살균력은 1.7× 10^(7) cells/㎖에 대해 5분간 자외선 조사를 하였을 경우 2.0× 10^(2) cells/㎖으로 감소하였고, 자외선 조사와 함께 슬러리 형태의 TiO_(2)를 첨가하였을 경우에는 3.4× 10 cells/㎖으로, 자외선 조사와 함께 TiO_(2)가 석영관에 박막증착된 경우에는 7.6× 10^(2) cells/㎖으로 감소하였다. 한편 위와 같은 조건에 유리관 하부에서 기포를 주입후 11분 동안 자외선을 조사시킨 경우에는 1.3× 10^(2) cells/㎖으로, 자외선 조사와 함께 슬러리 형태의 TiO_(2)를 첨가하였을 경우에는 1× 100 cells/㎖으로, 자외선 조사와 함께 TiO_(2)가 박막증착된 경우에는 7.9× 10 cells/㎖을 나타내었다. 결국 UV-TiO_(2) 반응기에 사용되는 TiO_(2)가 슬러리 형태일 때 최대 살균효과를 나타내었으나 기포가 첨가되었을때는 오히려 살균에 장애를 받는 것으로 나타난 반면 석영관에 박막증착된 경우에는 기포가 첨가되는 것이 살균에 효과적인 것으로 관찰되었다. The killing effect of UV-TiO_(2) photocatalytic system on the Escherichia coli DH5-慣 and Aspergillus oryzae var. oryzae (KCTC 6095) was studied. The UV lamp which emits maximum 14 watts at 254 nm was set on the center of pyrex round glass tube. Two types of TiO_(2), one of which is slurry and another which is thin film coated form, were used to determine the killing effect. When UV was irradiated to 1.7 * 10^(5) cells/??of E. coli for 11 min, the living cell number decreased to 4.0 * 1.0^(0) cells/?? The effect of UV system together with slurried TiO_(2) was less than 1 cells/?? whereas that of UV-coated TiO_(2) system decreased to 7.1 * 10^(3) cells/?? To study the effect of bubble on the killing of microorganisms, air was bubbled to the bottom of glass tube. When 1.7 * 10^(5) cells/??were exposed to UV for 11 min in combination with air bubble, the living cell number decreased to 1.3 x 10^(2) cells/?? In the same condition except the addition of slurried TiO_(2), the living cells were 1 * 10^(2) cells/?? However, more cells could be killed by the system which consists of UV, coated TiO_(2), and air bubble (7.9 * 10^(1) cells/??. From these results, it was found that UV-slurried system is the most effective one, but its killing effect is not stimulated by air bubble. However, bubbling was very effective in the UV-coated TiO_(2) system.

水稻의 生育時期에 따른 잎 損傷이 收量에 미치는 影響

金鎭雨,安元燮,金正洙,崔周鎬 진주산업대학교 1985 論文集 Vol.23 No.-

本 硏究는 水稻의 葉身損傷 時期및 程度가 收量과 玄米中의 無構質 含量에 미치는 影響을 究明하기 위하여 1985年 4月부터 1985年 10月동안에 걸쳐서 葉身損傷 時期를 8月2日, 8月12日, 8月22日로 하고 損傷程度는 止葉을 포함한 上位4枚의 葉을 完全切葉, 半切葉, 半折返으로 水稻의 收量 玄米의 無構質 含量을 調査한 것으로서 그 結果를 要約하면 다음과 같다. 1. 登熟比率과 精玄比率은 葉身의 處理時期가 늦을수록 높았고 損傷程度에서는 공히 半折返區에서 高度의 有意性이 認定되었다. 2. 玄米重量및 正租收量은 處理時期가 늦은 半折返區에서 가장 높았다. 3. 秕重과 重層未은 處理時期와 損傷程度間에 高度의 有意性이 認定되었지만 稈長, 穗長, 穗數, 粒數 및 玄米千粒重에서는 有意性이 認定되지 않았다. 4. 玄米의 無構質 含量은 T-N K_2O는 完全切葉區에서 半切葉區, 半折返區보다 약간 높았고 P_2O_5 CaO MgO SiO_2는 공히 半切葉區에서 完全切葉區와 半折返區보다 높았다. 處理時期로는 T-N性分은 時期가 가장늦은 8月22日區에서 약간 높았고 P_2O_5 CaO MgO K_2O는 공히 處理時期가 가장 빠른 8月 2日區에서 많았다. SiO_2만은 8月 12日區에서 多少 높았다. This experiment was conducted to find out the degree and time of leafblade in jury of rice affects on yield and quality of rice grain. The investigation was complied from April, 1985 to October, 1985. To investigate the yield of rice and quality of rice grain. The leaves were injured by the three Types of treatments like full cutting, half cutting and bending the each 4 leaves locatea in the top portion of rice including flag leaf at three different time August 2, August 12 and August 22. According to the analysis of the date collected in this experiment, the main result obtained was a follows; 1. The later the treatment, the more the percent of ripeness and the percentage of hulled and unhulled rice increased. And the degree of injury showed high significance in bending plot. 2. Brown rice weight and winnowed rice yield showed the highest rate in late treatment of bending plot. 3. Empty grains weight and rice screenings weight showed high significance between the treatment time and degree of injury but not in culm length, panicle length, panicle number per plant, number of grains per spikelet and brown rice 1,000 grain weight. 4. The result of an analysis of the quality of rice grain showed that full cutting plot produced greater quantity than half cutting plot and bending plot in the content of T-N, K_2O. But in the content of p_2O_5, CaO, MgO, SiO_2, half cutting plot showed greater quantity than full cutting plot and bending plot. On the other hand as for the treatment time the ingredient of the T-N showed a little higher rate in the plot of August 22 with latest treatment time. But the ingredient of the P_2O_5, CaO, MgC, K_2O showed high rate in the plot of August 2 with the earliest treatment time. But only SiO_2 showed a little higher rate in the plot of August 12.