FK506이 T 림프구 사멸에서 활성산소 생성에 미치는 영향

이호균(Ho Kyun Lee),정상영(Sang Young Chung),최수진나(Soo Jin Na Choi) 대한외과학회 2009 Annals of Surgical Treatment and Research(ASRT) Vol.77 No.5

Purpose: Tacrolimus (FK506) has been widely used as an immunosuppressant in organ transplanted recipients to suppress organ rejection phenomenon. We investigated the role of oxidative stress and heme oxygense-1 by FK506 on human Jurkat T cells. Methods: The cells viability was examined by DAPI stain, enzyme activity of caspase family proteins, and western blotting for Baks, PUMA, iNOS, HO-1. Cells were cultured in the absence or presence of CoPPIX or ZnPPIX and the fluorescence intensity was analyzed using a flow cytometry. Results: Treatment with FK506 increased the generation of reactive oxygen species (ROS), including hydrogen peroxide and superoxide anion, and NO in Jurkat cells in a dose-dependent manner. Immunohistochemistry and Western blot analysis data revealed the hemoxygenase-1 (HO-1) was induced by the addition of FK506 in Jurkat cells. Induction of CoPP, HO-1 inducer, resulted in decreased intracellular H₂O₂ and NO concentrations. Instead ZnPP, an HO-1 competitive inhibitor did it reversely. In addition, ZnPP regulates iNOS protein synthesis by inhibition of HO-1. Conclusion: Increase of HO-1 expression would induce to decrease the intracellular H₂O₂ and NO concentrations. Also, HO-1 would regulate iNOS protein synthesis. Consequently, we can expect the regulation of HO-1 expression with concomitants use of FK506 to suppress organ rejection phenomenon by enhancing apoptosis.

Mycophenolic Acid에 의해 유도된 Jurkat 세포주 세포자멸사에서 Heme Oxygenase-1의 발현이 미치는 영향

이호균(Ho Kyun Lee),최수진나(Soo Jin Na Choi) 대한외과학회 2010 Annals of Surgical Treatment and Research(ASRT) Vol.78 No.6

Purpose: This study demonstrates that pharmacologic induction of heme oygenase-1 (HO-1) along with catalytic activation significantly modulated apoptosis of Jurkat cells induced by mycophenolic acid (MPA). Methods: Cells were cultured with the presence or absence of MPA. Flow cytometric analysis was performed after propidium iodide staining. Western blotting of HO-1, Bcl, and Bax was also performed. Cells were stained 4’-6-Diamidino-2-phenylindole (DAPI) and measured by flow cytometry in the absence or presence of CoPPIX. Results: Treatment of MPA decreased cell viability in a dose- and time-dependent manner. MPA-induced cell death was confirmed as apoptosis characterized by sub G0/G1 phase arrest. Expression of HO-1 assumes a pattern of decline after rising at the initial phase. CoPPIX, HO-1 inducer, significantly inhibited the cisplatin-induced apoptosis. Treatment of MPA resulted in reactive oxygen species (ROS) generation in Jurkat cells. CoPPIX attenuated ROS production in MPA-treated cells. Conclusion: This result suggests that the protective mechanism of HO-1 on MPA-induced cytotoxicity is associated with direct inhibition of ROS generation and mitochondrial permeability transition.

조구등(釣鉤藤)의 4-VO로 유발한 흰쥐뇌허혈에 대한 신경방어효과

李仁煥,林康鉉,李鍾錫,石庚浩,安德均,朴虎君,김頀哲 대한본초학회 1999 大韓本草學會誌 Vol.14 No.2

Uncariae Ramulus et Uncus(UR, ???) has sweet in flavour and slightly cold in property, acting on the liver and pericardium channels. This drug was described in a medical classic as having the ability to remove "heat", check hyperfunction of the liver and relieve dizziness, tremors, and convulsion, and subdue "endogenous wind". So this study was planned to check the neuroprotective effect of UR on the global ischemia induced by 4-vessel occlusion in Wistar rats. and UR extract was lyophilized after extraction with 70% methanol. We induced 4-vessel occlusion for 10 minutes and reperfused again. The number of CA1 pyramidal neurons were counted after 7 days of reperfusion under the cresyl violet staining. In 4-VO ischemia model, UR showed significantly neuroprotective effects(1,000 and 500 ㎎/㎏ of UR extracts, p<0.05) compared with control group. Each neuroprotective ratio was about 23.0%, 19.0% respectively. Consequently, Uncariae Ramulus et Uncus has neuroprotective effects on the global ischermia induced by 4-vessel occlusion in Wistar rats. So we expect that Uncariae Ramulus et Uncus can be used as a drug for neurodegenerative disease.

韓醫學으로 보는 文宗의 疑問死에 대하여

이종호(Lee, Jong-Ho),안덕균(Ahn, Duk-Kyun) 백산학회 2009 白山學報 Vol.- No.83

The authors explained the hypothesis that Moonjong of Lee's Dynasty maybe died by the unexplaining treatment of the Medical Officer named JeonSoonEu. Moonjong had very slight desease called swelling, but Medical Officer gave him very poisonous Pinellia ternata in Pheasant meat, So he suddenly died in the three year of reign means very short period among the Lee's Dynasty. Generally Pheasant like to eat Pinellia ternata during the Summer, So If one want to eat the Pheasant meat must waite till the winter. Because during the winter Pheasant can't found the Pinellia ternata, so Pheasant meat not toxic. ... Who is the orderer, are not yet reaveled instead of JeonSoonEu. One cann't give the confidence as a prime mover of JeonSoonEu, because he is only one of the Medical Officier. So one thinks that probably SeJo, the younger brother, one of the most powerful king during the Lee's Dynasty is the main suspecter. Sejo killed the nephew Danjong, Son and succesor of MoonJong, and catch the reins of government by 'coup d'etat’. If Sejo connect with the Moonjong's died, maybe he is the only one suverean who killed the two kings overall the Korean history.

해수에서의 알루미늄 합금의 부식 거동에 관한 연구

許振,李承哲,李浩均,李宰鎬 弘益大學校 科學技術硏究所 1997 科學技術硏究論文集 Vol.8 No.-

(O_R_V) which vaporizes the LNG to NG is consisted of the tube and the header whose substrate is aluminum alloy. The corrosion of the tube is very severe because of the sea water being used as the heating source. In this study to protect the (O_R_V) substrate material, the corrosion behaviors of several aluminum alloys have been investigated. Al1080, Al3003, Al5083, coextruded and thermally sprayed Al-Zn (<2wt%) and Al-Zn (<2wt%) aluminum alloys were prepared for the electrochemical and immersion tests, and the surface analysis. As the result of the investigations, the corrosion potential of Al-Zn (<2wt%) alloy is lower than that of Al3003 alloy regardless of the content of oxygen dissolved in the sea water. The same trend was also observed with time. The surface roughness and the adhesion of coextruded coating layer of Al-Zn (<2wt%) is superior to those of the thermally sprayed coating layer. From the results of the immersion test to observe the corrosion behavior of Al-Zn (<2wt%) aluminum alloy, the corrosion products has the double layer that is considered as the oxide or the hydroxides layer.

서울의 Penicillinase Producing Neisseria Gonorrhoeae 발생빈도(1996)

김재홍,황동규,전재홍,김윤석,김중환,김용준,이창균,임동진,김현수,조창근,김경문,박상훈,전우형,김희성,이호정,차명수,김갑형,김형석,김석우,황지환,박병순,권오상,이민수,송기훈,성소영,이인섭,부태성 대한화학요법학회 1999 대한화학요법학회지 Vol.17 No.2

Background : In recent years, gonorrhea has been panedemic and remains one of the most commom STDs in the world, especially in developing countries. Objective & Methods: For the detection of a more effective therapeutic regimen and assessing the prevalence of PPNG, we have been trying to study the patients who have visited the VD Clinic of Choong-Ku Public Health Center in Seoul since 1980 by means of the chromogenic cephalosporin method. Results: In 1996, 139 strains of N. gonorrhoeae were isolated, among which 53(39.0%) were PPNG. Conclusion: Our results suggests that after a peak of 74.3% in 1993, the prevalence of PPNG in Seoul is gradually declining.

Pichia pastoris로부터 Toll-like Receptor 9의 세포 내 도메인 단백질의 발현과 순수분리 정제

이균영,이곤호 Plant molecular biology and biotechnology research 2005 Plant molecular biology and biotechnology research Vol.2005 No.

Toll-like receptors (TLR) are important components of innate immunity in the defense against pathogens. TLRs recognize pathogen-associated common molecular patterns. TLRs are similar to the receptors involved in defense responses in plants. TLR protein is a type 1 membrane protein, consisting of an extracellular domain containing leucine-rich repeats and a cytoplasmic domain. The cytoplasmic domain delivers ligand recognition signals that result in production of anti-microbial agents. The cytoplasmic domain (amino acid 858-1032) of toll-like receptor 9 has been expressed using methylotrophic yeast Pichia pastoris. The protein expression was confirmed by Western-blot, N-terminal sequencing and MALDI-TOF mass spectrometry. The proteins have been purified by nickel affinity, cation exchange and gel-filtration chromatography.

杜仲과 木瓜의 混合 煎湯液이 흰쥐의 卵巢摘出로 유발한 骨多孔症에 미치는 影響

李鍾安,盧昇鉉,安德均,崔湖榮 대한본초학회 2001 大韓本草學會誌 Vol.16 No.1

The purpose of this study is to examine the effects of Eucommiae Cortex and Chaenomelis Fructus on the aged ovariectomized rat, similar model with postmenopausal osteoporosis. The results of the experiment are as follows, 1. The body weight in OVX/ECCF group showed a increase.. 2. The level of S-AST and S-ALT in OVX/ECCF group significantly reduced. 3. Urinary volume and creatinine in OVX/ECCF group reduced.. The level of urinary deoxypyridinoline in OVX/ECCF group fignificantly reduced. According to the above results, this experiment indicates that OVX/ECCF group stimulated born formation and diminished the loss of born marrow, then recovered the failure of liver function on ovariectomized rat. In conclusion, an extract of Eucommiae Cortex and Chaenomelis Fructus showed preventive and curing effects in treating osteoporosis of ovariectomized rats. This result suggests that it can be used in preventing senile and postmenopausal osteoporosis. As for its effects, continuous clinical study is needed.

황금(黃芩)의 4-VO로 유발한 흰쥐뇌허혈에 대한 신경방어효과

李秉哲,林康鉉,金榮玉,金善礪,安德均,朴虎君,金護哲 대한본초학회 1999 大韓本草學會誌 Vol.14 No.2

Scutellariae Radix(SR), the root of Scutellaria baicalensis G_EORGI, whose property, flavour and channel tropism is bitter in flavour, cold in property(寒), acting on the lung, gallbladder, stomach and large intestine channels(歸?,?,?,大??), has the effects of clearing away heat and dampness(淸熱??), purging fire, detoxicating(?火解?), stopping bleeding(止血) and preventing miscarriage(安?). This drug is one of the popular drugs in traditional Korean medicine, which has been used to antipyretic, antibacterial, antitoxic and antihypersensitive effects. So this study was planned to check the neuroprotective effect of SR on the global ischemia induced by 4-vessel occlusion in Wister rats. and SR extract was lyophilized after extraction with 70% methanol. We induced 4-vessel occlusion for 10 minutes and reperfused again. The number of CA1 pyramidal neurons were counted after 7 days of reperfusion under the cresyl violet staining. The result obtained that in 4-VO ischemia, SR showed significantly neuroprotective effects(1,000 and 500 ㎎/㎏ of SR extracts, p<0.05) compared with control group. Each neuroprotective ratio was about 27.4 %, 23.2 % respectively. Consequently, Scutellariae Radix has neuroprotective effects on the global ischemia induced by 4-vessel occlusion in Wistar rats.

이개강 전위피판을 이용한 외이도 입구 확장술

이호기,김희남,문성균,김은서,박남성 釜算慶南耳鼻咽喉科學會 1994 임상이비인후과 Vol.5 No.2