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      • High-performance Liquid Chromatographic Analysis for Quantitation of Marker Compounds of Artemisia capillaris Thunb

        ( Kyung Min Park ),( Ying Li ),( Bora Kim ),( Haiyan Zhang ),( Kyong Hwangbo ),( Dong Gen Piao ),( Mei Juan Chi ),( Mi Hee Woo ),( Jae Sue Choi ),( Je Hyun Lee ),( Dong Cheul Moon ),( Hyeun Wook Chang 영남대학교 약품개발연구소 2013 영남대학교 약품개발연구소 연구업적집 Vol.23 No.0

        Two stable high-performance liquid chromatography (HPLC) methods were developed that could quantitatively analyze 10 major marker compounds of Artemisia capillaris Thunb and could also distinguish among `Injinho` and `Myeon-injin` and `Haninjin`--A. capillaris collected in autumn, A. capillaris collected in spring and A. iwayomogi, which can be misused as `Injinho` in Korean herbal drug markets. The first HPLC method was a reversed-phase chromatography using a C18 column with an isocratic solvent system of phosphoric acid (0.05%) and acetonitrile at the flow rate of 1.0 mL/min, ultraviolet (UV) detection wavelength at 254 nm and column temperature at 40°C. Calibration andquantitation were made by using acetaminophen as an internal standard (I.S-A) and chlorogenic acid (1) was determined within 20 min. The second HPLC method was a reversed-phase chromatography using a C18 column with a gradient solvent system of phosphate buffer (0.015 M, pH 6) and acetonitrile at the flow rate of 1.0 mL/min, UV detection wavelength at 254 nm and column temperature at 40°C. Calibration and quantitation were made by using ethylparaben as an internal standard (I.S-B) and 3,5-di-O-caffeoylquinic acid (2), 3,4-di-O-caffeoylquinic acid (3), 4,5-di-O-caffeoylquinic acid (4), hyperoside (5), isoquercitrin (6), isorhamnetin 3-O-robinobioside (7), isorhamnetin-3-O-galactoside (8), isorhamnetin-3-O-glucoside (9) and scoparone (10) were determined within 60 min. Pattern recognitionanalysis of data from the 60 samples classified them clearly into three groups. These assay methods could be applied for QA/QC of A. capillaris and Artemisia iwayomogi.

      • KCI등재

        High-performance Liquid Chromatographic Analysis for Quantitation of Marker Compounds of Artemisia capillaris Thunb.

        Kyung Min Park,손종근,Ying Li,Bora Kim,Haiyan Zhang,Kyong Hwangbo,Dong Gen Piao,Mei Juan Chi,우미희,최재수,이제현,문동철,장현욱,김재룡 대한약학회 2012 Archives of Pharmacal Research Vol.35 No.12

        Two stable high-performance liquid chromatography (HPLC) methods were developed that could quantitatively analyze 10 major marker compounds of Artemisia capillaris Thunb and could also distinguish among ‘Injinho’ and ‘Myeon-injin’ and ‘Haninjin’ – A. capillaris collected in autumn,A. capillaris collected in spring and A. iwayomogi, which can be misused as ‘Injinho’ in Korean herbal drug markets. The first HPLC method was a reversed-phase chromatography using a C18 column with an isocratic solvent system of phosphoric acid (0.05%) and acetonitrile at the flow rate of 1.0 mL/min, ultraviolet (UV) detection wavelength at 254 nm and column temperature at 40oC. Calibration and quantitation were made by using acetaminophen as an internal standard (I.S-A) and chlorogenic acid (1) was determined within 20 min. The second HPLC method was a reversed-phase chromatography using a C18 column with a gradient solvent system of phosphate buffer (0.015 M, pH 6) and acetonitrile at the flow rate of 1.0 mL/min, UV detection wavelength at 254 nm and column temperature at 40oC. Calibration and quantitation were made by using ethylparaben as an internal standard (I.S-B) and 3,5-di-O-caffeoylquinic acid (2), 3,4-di-O-caffeoylquinic acid (3), 4,5-di-O-caffeoylquinic acid (4), hyperoside (5), isoquercitrin (6), isorhamnetin 3-O-robinobioside (7), isorhamnetin-3-O-galactoside (8), isorhamnetin-3-O-glucoside (9) and scoparone (10) were determined within 60 min. Pattern recognition analysis of data from the 60 samples classified them clearly into three groups. These assay methods could be applied for QA/QC of A. capillaris and Artemisia iwayomogi.

      • Cutting performance of tungsten carbide tools coated with diamond thin films after etching for various times

        Kim, Jong Seok,Park, Yeong Min,Bae, Mun Ki,Kim, Chi Whan,Kim, Dae Weon,Shin, Dong Chul,Kim, Tae Gyu World Scientific Publishing Company 2018 Modern physics letters. B, Condensed matter physic Vol.32 No.20

        <P>In this study, diamond thin films were deposited on tungsten carbide tools using surface-wave plasma-enhanced chemical vapor deposition (SWP-CVD). To eliminate the adverse effects of cobalt on the diamond deposition process, the cobalt was removed from the surface of the tools by etching with Murakami’s reagent for various times (30, 60, and 120 min). The cutting performance of the untreated and the diamond-coated WC tools was examined by performing cutting test on carbon fiber-reinforced plastic (CFRP). The results showed that all the diamond-coated tools exhibited great improvement on the durability and wear resistance compared to the uncoated one. In addition, the diamond-coated tool lift time is found to be proportional to the etching time. An increase more than twofold has been achieved when the etch time was increased from 30 min to 120 min.</P>

      • KCI등재

        도심 수목원에서의 경관감상과 산책이 인체의 생리적 안정에 미치는 영향

        박범진 ( Bum Jin Park ),가재남 ( Jae Nam Ka ),이민선 ( Min Sun Lee ),김선아 ( Seon A Kim ),박민우 ( Min Woo Park ),최윤호 ( Yoon Ho Choi ),정다워 ( Da Wou Joung ),권치원 ( Chi Weon Kwon ),염동걸 ( Dong Geol Yeom ),박순주 ( Soon Jo 한국산림과학회 2014 한국산림과학회지 Vol.103 No.4

        This research was conducted to investigate the impact of viewing scenery and walking in the urban forest onphysiological relaxation of humans. The experiment was conducted in Hanbat Arboretum located in Daejeon, and thecontrol experiment was conducted in front of Daejeon City Hall. The subjects that participated in the experimentcomprised 24 Korean male university students in their 20s (average age, 21.1±2.5 years), participated in the experimentas the subject, and these subjects were classified into three groups divided into three locations such as the pine forest inArboretum, the pond in Arboretum, and the city. The subjects sat down and viewed scenery for 10 min, and they thenwalked for 15 min. Further, physiological changes were measured using indicators such as heart rate variability (HRV),blood pressure, and pulse rate. As a result, when subjects viewed scenery and walked in the urban forest, a statisticallysignificant increase in the high frequency (HF) power of HRV and decrease in systolic pressure was observed comparedwith when subjects viewed scenery and walked in the city. Through this research, it was found that activities like viewingscenery or walking in Arboretum of the city are effective in increasing the physiological relaxation of the city residents.

      • 경북 천생산, 유학산, 숲데미산지역의 식물군락유형

        박강민,김종현,강치욱,이종운 嶺南大學校基礎科學硏究所 2001 基礎科學硏究 Vol.21 No.-

        From April to October 2001, we studied on vegetation of 60 plots through the methods of Braun-Blanquet at Cheonsaengsan, Yuhaksan and Supdemisan in Gyeongbuk. Through the methods of interspecific affinity analysis, DECORANA and TWINSPAN the patterns of plant communities of 60 were analysed. It showed 6 communities at Cheonsaengsan, 5 communities at Yuhaksan, 6 communities at Supdemisan and total appearance of 128 species and covered with 50∼95% of tree layer, 20∼90% of shrub layer, 20∼90% of herb layer. As the result of interspecific affinity analysis it showed one big specific complex composed with 52 species and the 60 plots were divided into 4 groups by classification and ordination. One big species complex as a result of affinity analysis and eigenvalue of 0.303 at first step by classification indicate that Cheonsaengsan, Yuhaksan and Supdemisan are in developing stage of succession.

      • KCI등재
      • 경북 유학산, 천생산과 성암산 일대의 식물군락유형 분석

        박강민,김종현,정현아,강치욱,이종운 嶺南大學校基礎科學硏究所 2002 基礎科學硏究 Vol.22 No.-

        At the area of Yuhak, Cheonsaeng and Seongam mountains in Kyeongbuk, we studied the vegetation patterns with 75 plots on the base of Braun-Blanquet methods from March to October 2002. For the analysis of community patterns with 75 plots we used interspecies Affinity analysis method, DECORANA and TWINSPAN. The investigated area was divided into 17 communities with 177 species, and 20∼100% of treelayer, 10∼95% of shrublayer and 20∼100% of herblayer. By toe method of affinity analysis, it showed a big community with 86 species, but divided into 6 small groups by ordination and classification methods. Although it appeared as one group by the affinity test, the area divided into two groups by classification method at second level with eigenvalue of 0.320 and 0.430. It shows that the area of Yuhak, Cheonsaeng and Seongam mountains have own properties and in the process of succession.

      • 고강도 콘크리트 디스크를 이용한 혼합모드 파괴에 관한 연구

        진치섭,김희성,박현재,김민철 부산대학교 생산기술연구소 2000 生産技術硏究所論文集 Vol.59 No.-

        RILEM 위원회가 제시한 3점 휨 시험은 하중-변위곡선 형상이 불규칙하고 안정된 균열 발생 후 최종균열이 발생하기 때문에 정확한 파괴인성을 구하는 것은 어렵다. 그러나, 디스크 시험은 균열 개시 하중만 알면 쉽게 파괴인성을 구할 수 있다. 중앙에 노치가 있는 고강도 콘크리트 디스크를 실험하여 실험결과와 유한요소에 의한 결과를 비교하였다. 또한, 실험에 의한 파괴 포락선과 이론에 의한 파괴 포락선도 비교하였다. It is difficult to obtain accurate fracture toughness values by RILEM committees proposed three point bend test(TPB) because the shape of load-deflection curve is irregular and final crack propagation of occurs after some slow stable cracking. However, for disk test, fracture toughness is easily obtained from crack initiation load. We examined the center notched high strength concrete disk and the experimental results were compared with the results by finite element analysis(FEA). Also, we compared experimental

      • 경북 천생산, 유학산, 숲데미산지역의 현존식생 및 식물군락

        김종현,박강민,강치욱,이종운 嶺南大學校基礎科學硏究所 2001 基礎科學硏究 Vol.21 No.-

        From April to October 2001, we studied on vegetation of 60 plots through the methods of Braun-Blanquet at Cheonsaengsan, Yuhaksan and Supdemisan in Gyeongbuk. In whole area of Cheonsaengsan, Yuhaksan, Supdemisan we gave the grades based on the criteria of ministry of environment, and described an actual vegetation map based on physiognomy. Through the methods of Braun-Blanquet it showed 6 communities at Cheonsaengsan, 5 communities at Yuhaksan, 6 communities at Supdemisan and total appearance of 128 species and covered with 50∼95% of tree layer, 20∼90% of shrub layer, 20∼90% of herb layer. The vegetation in actual vegetation map divided by physiognomy were 8 communities at Cheonsaengsan, 8 communities at Yuhaksan, 10 communities at Supdemisan. The vegetation with more than vegetation grade Ⅲ was not appeared and it means that this area needs continuous conservation plan.

      • PVD 방법에 의한 TiN barrier metal 형성과 공정개발

        염병렬,박형호,최치규,이종덕,김건호,이정용,서경수,강민성 濟州大學校 基礎科學硏究所 1997 基礎科學硏究 Vol.10 No.2

        Ar과 N₂?가스가 혼합된 분위기에서 반응성 스퍼터링 방법에 의하여 TiN 박막을 증착하였다. N₂ 가스의 농도는 화학양론적으로 TiN이 형성되는 조건에 맞도록 조절하였으며, 기판의 온도는 실온에서 부터 700℃의 범위내로 유지하였다. (111) texture 구조를 가지면서 화학양론적으로 Ti??N??인 박막은 기판의 온도가 600℃ 이상에서 형성되었고, 기판의 온도가 600℃ 이하에서는 형성된 박막은 N-과다형이었다. XRD, XPS 및 RBS 분석 결과 TiN 박막의 조성비는 기판의 온도에서 다소 의존하였으나 약 5% 이내에 불과하였다. TiN 박막의 면저항은 기판온도의 증가에 따라 감소하였고, 기판온도가 600℃에서 증착된 TiN 박막의 면저항능 14.5Ω/□ 였고, Ar―가스 분위기에서 700℃로 30초간 열처리한 후는 8.9Ω/□ 이었다. 따라서 반응성 스퍼터링방법에 의하여 형성되는 양질의 TiN 박막은 기판온도가 600℃이상이 최적조건임을 알았다. Titanium nitride (TiN) films were prepared by reactive sputter deposition in mixed gas of Ar+N₂?. The volume percentage of N₂ in the working gas was chosen so as to grow stoichiometric TiN films and the substrate temperature during film growth was set from room temperature to 700℃. stoichiometric Ti??N?? films with (111) texture were grown at temperatures over 600℃, while films prepared at temperatures below 600℃ showed N-rich TiN. The composition x and y in the Ti??N?? films determined by XPS and RBS varied within 5% with the substrate temperature. The sheet resisstance of the TiN films decreases as the substrate temperature increased. TiN film prepared at 600℃ showed 14.5Ω/□, and it decreased to 8.9Ω/□ after the sample was annealed at 700℃, 30 sec in Ar-gas ambient by RTA. By far, high quality stoichiometric TiN films by reactive sputtering in the mixed gas ambient could be prepared at substrate temperature over 600℃.

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