방사선조사가 백서 법량질형성 과정에 미치는 영향에 관한 연구

최순철,유동수,최현배,박태원 大韓口腔顎顔面 放射線學會 1997 Imaging Science in Dentistry Vol.27 No.1

The purpose of this study was to investigate the radiation effect on the stages of amelogenesis. Twenty 11-day-old rats which were irradiated by 4Gy of gamma radiation on the 19th prenatal day were used for the experimental group and twenty 11-day-old rats which were not irradiated were used for the control group. The length of each zone of amelogenesis were measured on the sagittal section using a light microscopic enlargement at 400 x the normal view while the morphologic changes of ameloblasts of each zone were observed electron-microscopically. The obtained results were as followed: 1. The length of the region of facing pulp and facing dentin of the zone of presecretion were increased by 11.5%(p<0.05) and 17.7%(p<0.01), respectively. 2. The length of the zone of secretion was increased by 17.3%(p<0.01), but the zone of maturation was decreased by 15.3%(p<0.01). 3. The total length of the zone of amelogenesis was not changed significantly(p>0.05). 4. Electron-microscopically, enlargement of the cell membrane, rER, mitochondria, and nuclear membrane were observed. These changes were mostly severe in the zone of maturation.

Mycobacterium fortuitum-chelonei complex 균체항원의 민감성과 특이성

이현우,최철순,정상인,양용태 중앙대학교 의과대학 의과학연구소 1985 中央醫大誌 Vol.10 No.1

Antigenic preparations were made from seven strains of Mycobacterium fortuitum-chelonei complex(MFCC) extracted with phosphate buffered saline(PBS extracts), with 10%, trichloroacetic acid solution (TCA extracts) and with veronal buffered saline solution containing 0.1% Triton X-100(Triton extracts). Serologic reactivities of the antigenic preparations were assayed for immune precipitation by Ouchterlony double diffusion in-gel technique against antisera of rabbits immunized with M. fortuitum TMC 1529 and M. chelonei TMC 1544. Antigenic relationships between Triton extracts of M. fortuitum and M. chelonei were analyzed by two-dimensional immunoelectrophoresis(2D-IE) and fused rocket immunoelectrophoresis(FRIE). The results obtained are summarized as follows: 1. Of antigenic preparations of PBS extracts, TCA extracts and Triton extracts, the Triton extracts and the PBS extracts proved to be active for immune precipitation, but the TCA extracts was inactive. The precipitation reaction of Triton extracts was more sensitive than PBS extracts, and the precipitation reaction of Triton extracts was enhanced by disintegration of cells with sonification, but the cross reaction was also increased. 2.' The Triton extracts of 5 strains of M. fortuitum exhibited 9 common precipitinogens with reference serum against TMC 1529 and one common precipitinogen with reference serum against TMC 1544. but the antigens of TMC 1544 with or without disintegration of cells gave a specific precipitinogen with reference serum against TMC 1544 in addition to a common precipitinogen. 3. Although the electrophoretic patterns of Triton extracts obtained from different strains and species were not demonstrated, 7 strains of the MFCC could be divided into four groups by the relative electrophoretic mobilities(REM) of the antigens with respect to bovine serum albumin(REM value); group Ⅰ(REM =0.18) 2 strains, group Ⅱ(REM = 0.27) 2 strains, group Ⅲ (REM = 0.38) 2 strains and group Ⅳ(REM = 0.49) 1 strain, but the existence of any significant relationship between the REM value and species was not recognized. 4. By means of FRIE, the presence of 3 to 5 common precipitinogens and 1 to 2 species-specific precipitinogens were observed in the Triton extracts of M. fortuitum and M. chelonei. 5. By the use of the reference antisera with a titre of at least 1 : 80 against M. fortuitum TMC 1529 and M. chelonei TMC 1544, the strains of M. fortuitum and M. chelonei could be differentiated by FRIE. In conclusion, this study established that the Triton extracts of the MFCC proved to be active for immune precipitation and contained species-specific procipitinogens, and reproducible amounts of such an antigen for immunodiffusion test could be easily prepared with one plate of heavy cultures.

상악동의 점막비후에 대한 WATERS방사선사진과 파노라마방사선사진 및 전산화단층사진의 진단능 비교

최순철,이삼선,현영민 大韓口腔顎顔面 放射線學會 1998 Imaging Science in Dentistry Vol.28 No.1

With the CT findings as gold standard, the sensitivity, the specificity, and the diagnostic accuracy of Water's radiography and Waters' radiography with panoramic radiography were compared in the diagnoses of antral mucosal thickening of 16 patients. Three oral radiologists and three non-oral radiologists interpreted the Waters' radiographs and after 4 weeks, interpreted the Waters' radiographs and panoramic radiographs simultaneously. The interpretation point was the existence or the non-existence of the mucosal thickening on the medial, the posterolateral, the floor, and the roof of maxillary sinus. The obtained results were as followed: 1. In oral radiologist group, the sensitivity, specificity and diagnostic accuracy of Waters' film were 0.7250, 0.8489 and 0.7578 respectively. 2. The sensitivity and the diagnostic accuracy in oral radiologist group were higher than those of non-oral radiologist group (P<0.05), but there was no significant difference between two groups in the specificity (P>0.05). 3. There was no significant difference of the diagnostic abilities except the specificity in oral radiologist group between Waters' radiography and Waters' radiography with panoramic radiography (P>0.05). 4. The sensitivity and the diagnostic accuracy were the highest in the case of medial wall interpretation, the specificity was the highest in the posterolateral wall. 5. In the posterolateral wall and the floor, the sensitivity and the diagnostic accuracy of oral radiologist group were higher than those of non-oral radiologist group (P<0.05).

식물플라크톤의 세포외배출유기물을 고려한 소양호의 1차생산과 유기물 부하

남궁현,김범철,황길순,최광순,김철구 江原大學校 附設 環境硏究所 2001 環境硏究 Vol.18 No.-

The autochthonous and allochthonous organic carbon loading were measured in Lake Soyang, to estimate the amount of carbon loading into the lake and the contribution of their sources to the lake's carbon loading. Autochthonous carbon loading was estimated from phytoplankton primary production with the extracellular organic carbon(EOC). Allochthonous loding was determined by measuring dissolved organic carbon(DOC) and particulate organic carbon(POC) concentration in the main inflowing Soyang River. Both autochthonous and allochthonous organic carbon loading were high during the summer, from July to September, and accounted for 43.2% and 71.7% of the annual loading, respectively. Primary productivity was elevated up to 1,000 mgCm^(-2)d^(-1)during summer and lowest in winter. EOC production from phytoplankton was also large in summer, resulting in a high DOC concentration in the lake water. Primary production of phytoplankton and allochthonous organic matter loading from the watershed contributed to 53.6% and 46.4% of total loading, respectively. The EOC production accounted for 4.4~21.2% of POC primary production, implying that EOC production of phytolankton must be considered in estimation of primary production.

몇가지 β-lactam 항생제의 약효평가를 위한 Minimum Inhibitory Concentration(MIC)의 비교

洪淳德,金鐘國,李東善,河相哲,李相瀚,李載蘭,安賢淑 慶北大學校 1991 論文集 Vol.51 No.-

In order to screen the new β-lactam antibiotics for clinical use. we were compared MIC values of various β-lactam antibiotics. We also tested the influence of pH, inoculum size, stability of antibiotics in medium and some kinds of media on MIC value. As a result, cefotaxime, cefoperazone, piperacillin, cefamandole, cephaloridine, cephapirin, cefazolin, cephalothin, cephaioylycn were scored in antimicrobial activity of MIC data.

투기종목 선수들의 무산소성 역치의 특성

조현철,송순천,김춘경,김의영,안용규,이규석,김학렬 龍仁大學校 體育科學硏究所 1991 體育科學硏究論叢 Vol.1 No.1

The purpose of the present study is to determine the levels of maximal oxygen uptake and anaerobic threshold of boxing players(n=23), ken-do players(n=18), Judo players(n=12), T.D.K players(n=18). Treadmill test was used to measure maximal state and anaerobic threshold of metabolic variables. Treadmill exercises protocol was 2-min incremental test from an initial work load of 0MPH(grade : 0%) to all-out by step of 1.0MPH(grade : 2%). The results of measurement was as follows : 1. Absolute(1/min) and relative(ml/kg/min) maximal oxygen uptake was highest(5.08l/min, 65.01 ml/kg/min) in Judo layers, followed in order by the T.D.K players (4.37l/min, 64.74 ml/kg/min), Boxing players(4.18) l/min, 63.19 ml/kg/min) and ken-do players(3.81l/min, 58.65 ml/kg/min). It is not significant difference in the relative maximal oxygen uptake, but is significant difference between group in the absolute maximal oxygen uptake(p<0.001) 2. AT-VO₂(l/min) and AT-VO₂(ml/kg/min) in the anaerobic threshold was highest (3.71 l/min, 47.28 ml/kg/min) in Judo players, followed in order by the T.K.D players(3.29 l/min, 48.75 ml/kg/min), boxing players(2.90 l/min, 43.91 ml/kg/min) and ken-do players(2.82 l/min, 43.52 ml/kg/min). It is not significant difference in the AT-V02(ml/kg/min), but is significant difference between group in the AT-V02(l/min). (p<0.001) 3. %HRmax and %V 02max in the anaerobic threshold was not significant difference between group.

성장발육에 따른 흰쥐의 악관절 신경분포의 변화

배용철,김영진,박국필,김현정,남순현 大韓小兒齒科學會 1999 大韓小兒齒科學會誌 Vol.26 No.1

The purpose of this study was to investigate the distribution of CGRP immunoreactive nerve fibers in rat squamosomandibular joint with postnatal development. Squamosomandibular joint with adjacent tissue of 8 groups of rats(1,5,10,15,20,25,35-postnatal day and adult groups) were removed en bloc and processed for immunohistochemistry and were subjected to light microscopic examination. The results obtained were as follows; 1. The anterior portion of the articular disk was most densely innervated, followed by the posterior, lateral, and medial portions in each group. 2. Increase of CGRP immunoreactive nerve fibers was evident in 10,15,20 postnatal day groups. 3. Almost no CGRP immunoreactive nerve fibers were observed in articular surface of disk proper, mandibular fossa and condyle head of each group. These result suggest that CGRP immunoreactive nerve fibers increased in synovial membrane and peripheral portion of articular disk during 10-20 postnatal day may play a important role in squamosomandibular joint function after weaning period.

파노라마방사선사진 지수와 임플란트 실패와의 관계에 관한 연구

조현정,이원진,허민석,안창현,이진구,이삼선,최순철 大韓口腔顎顔面 放射線學會 2004 Imaging Science in Dentistry Vol.34 No.1

Purpose : Several panoramic indices have been suggested to assess bone quality from the morphology and width of mandibular cortex on panoramic radiography. The purpose of this study was to compare dental implant failure group with control group in panoramic mandibular index (PMI), mandibular cortical index (MCI), and gonion index (GI) and to determine the effect of these panoramic indices on dental implant failure. Materials and Methods : A case-control study was designed. Test group (n = 42) consisted of the patients who had their implants extracted because of peri-implantitis. Control group (n = 139) consisted of the patients who retained their implants over one year without any pathologic changes and had been followed up periodically. They had dental implants installed in their mandibles without bone augmentation surgery from 1991 to 2001. The following measures were collected for each patients: 1) PMI, MCI, and GI were measured twice at one-week interval on preoperative panoramic views; and 2) age, sex, implant length, implant type, installed location, occluding dentition state, and complication were investigated from the chart record. Results : The PMI showed moderate level of repeatability. The intra-observer agreement of MCI and GI were good. There was statistically significant difference in PMI between two groups. There were significant different patterns of distribution of MCI and GI between two groups. Among the panoramic indices, PMI and MCI showed significant correlation with dental implant failure. Conclusion : Panoramic indices can be used as reference data in estimating bone quality of edentulous patients who are to have implants installed in their mandibles.

니페디핀이 암피실린의 흡수에 미치는 영향

정현정,용철순,최윤수,오두만 영남대학교 약품개발연구소 1997 영남대학교 약품개발연구소 연구업적집 Vol.7 No.-

Amino-β-lactam antibiotics are absorbed by the dipeptide transporter in the small intestine. These uptakes are coupled to a proton influx. The inward proton gradient is partly induced by the Na^(+)/H^(+) exchanger and cakium ion is involved in control of this antiport. Interaction between ampicillin which is one of the amino-β-lactam antibiotics and nifedipine which is one of calcium channel blocking agents was studied in rats in vivo and with rabbit jejunum mounted on the Sweetana/Grass diffusion cells in vitro. Bioavailability of ampicillin was increased significantly when nifedipine was co-administered orally in rats. There were no differences in the distribution phase and the elimination phase when ampicillin was given either alone or with nifedipine intravenously. Conditions for in vitro experiments were determined. The lift rate of O₂/CO₂ gas was controlled to 3 bubbles/sec and ampicillin was stable in the Kreb's buffer at pH6.0 Absorption of ampicillin was the greatest when the completely-stripped serosal membrane was used. Transport of ampicillin from mucosal to serosal side in the rabbit jejunum was enhanced by 32% in the presence of nifedipine (p=0.059). Above results suggest that nifedipine might increase the plasma level of ampicillin via the improved absorption in the intestine rather than the reduction in the elimination or/and alteration in the distribution.

광합성 세균에 대한 연구 : II. 야생형 Rhodos pirillum rubrum에 의한 수용성 색소의 생산

오덕철,이현순 제주대학교 1984 논문집 Vol.17 No.-

1. Korean strain of wild type of Rhodospirillum rubrum excretes water-soluble pigments into the medium when grown anaerobically under light. 2. The absorption spectrum of the supernatant medium shows peaks at 392.5 (main peak), 500,535 mp together with shoulders at 370, 555 mp. 3. Water-soluble pigments show pinkish-orange fluorescence under short-wave ultraviolet light. 4. The optical density of the supernatant medium increases markedly with time when aerated under dark, room temperature. 5. The culture of R. rubrum shows no distinct differences in the excretion of water-soluble pigments when cultured in orthophosphate or pyrophosphate medium as inorganic phosphate source. 6. It is postulated that the water-soluble pigments excreted by the wild type of R rubrum are mixture of a few kinds of porphyrins.