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Synergistic effect of ulipristal acetate and vitamin D with HIFU on uterine leiomyoma
( Somi Oh ),( Ku Heon Kwon ),( Jeonghun Yeom ),( Kyunggon Kim ),( Yong-il Kwon ) 대한산부인과학회 2020 대한산부인과학회 학술대회 Vol.106 No.-
Objective: Uterine leiomyoma (UL), are noncancerous growths of the uterus that often appear during childbearing years and also called uterine fibroids. Recently, combination of ulipristal acetate (UPA) and high intensity focused ultrasound (HIFU) have been applied on UL treatment and cholecalciferol have been also suggested as a synergistic component but no pathway of efficacy was investigated. Herein, comparative proteome approach on leiomyoma cell line model was applied to investigate related pathways for UA treatment based on HIFU. Methods: Uterine leiomyoma cell line (GM10964) was treated with 10uM of ulipristal acetate (UPA) or with 10uM of UPA and 1uM of cholecalciferol(VitD3), respectively. After 24-hour incubation, cell was treated with 200W of HIFU using JC200D with home-made mount on transducer. And quantitative proteome analysis for each cell was performed using high resolution LC-MS platform. Results: Totally 5,286 proteins are identified among groups and gene ontology analysis showed that proteins which increased in HIFU combined with UPA and VitD3 were involved in mitochondrial metabolism, inferring apoptosis for cell death. Conclusion: Comparative proteome analysis revealed that combination of UPA and VitD3 on HIFU treatment for uterine leiomyoma showed the synergistic effect in term of apoptosis caused by mitochondrial metabolism.
삼백초 추출물이 항산화활성과 멜라닌 합성에 미치는 영향
정소미(Somi Jung),박혜정(Hyejeong Park),김재호(Jaeho Kim),오영희(Yunghee Oh),김문무(Moon-Moo Kim) 한국생명과학회 2020 생명과학회지 Vol.30 No.10
뿌리, 잎, 꽃이 하얀색인 삼백초는 항균 활성 및 항암 효과를 갖는 것으로 알려져 있다. 본 연구에서는 SCEA의 항산화 활성과 멜라닌 합성에 미치는 영향을 조사하고자 하였다. SCEA를 헥산, 에틸 아세테이트, 부탄올 및 증류수로 분획 하였다. 용매 분획 중 가장 높은 항산화 활성을 갖는 ethyl acetate 분획물이 본 연구에 사용되었다. DPPH radical 소거능에서는 64 μg/ml의 SCEA는 양성 대조군과 비교하여 62% 라디칼 소거능을 보였다. 환원력시험에서 64 μg/ml의 SCEA는 양성 대조군으로 사용된 비타민 C보다 33% 더 낮은 감소력을 보였다. Tyrosinase 활성은 공시험군에 비해 64 μg/ml의 SCEA 처리군에서 26% 증가했다. 또한, 64 μg/ml의 SCEA는 Dopa 산화력 분석에서 공시험군과 비교하여 44%의 멜라닌 합성을 증가시켰다. MTT 분석 결과 SCEA는 0.5 μg/ml 이상의 세포 독성을 나타냈다. 1 μg/ml의 SCEA는 멜라닌 합성을 살아있는 세포에서 69% 증가시켰다. 또한 SCEA 분획을 실리카 칼럼 크로마토그래피로 13 개의 분획으로 분리 하였다. 특히, Fr. 2는 DPPH 라디칼 소거 활성이 가장 높았으며, 멜라닌 합성이 감소되었다. 또한 SCEA는 살아있는 세포인 B16F1에서 멜라닌 생성을 촉진시켰다. LCMASS는 Fr. 2의 분자량이 239임을 보여 주었다. 따라서 위의 결과는 SCEA가 멜라닌 합성 촉진과 관련된 천연흑모 모발화장품 개발에 사용될 수 있음을 시사한다. Saururus chinensis has white roots, leaves, and flowers and is known to have antibacterial activity and anti-cancer efficacy. The aim of this study was to investigate the effect of the ethyl acetate fraction of a methanol extract of Saururus chinensis (SCEA) on antioxidant activity and melanin synthesis. SCEA at 64 μg/ml showed 62% of the DPPH radical scavenging activity of vitamin C, and its reducing power was 33% greater than that of vitamin C. Tyrosinase activity was 26% higher and melanin synthesis was 44% higher in the presence of SCEA at 64 μg/ml than in a blank group in a dopa oxidation assay. MTT assay showed that SCEA displayed cytotoxicity above 0.5 μg/ml, and SCEA at 1 μg/ml increased melanin synthesis by 69% in live B16F1 cells. SCEA was also separated into 13 fractions by silica column chromatography, and fraction 2 (Fr. 2) showed the highest DPPH radical scavenging activity, reducing power, and melanin synthesis. SCEA also promoted melanin production in live cells. LC-MASS analysis showed that Fr.2 had a molecular weight of 239, and these findings suggest that SCEA could be available for the promotion of melanin synthesis in black hair.
( Thanh Thi Nguyen ),( Somy Yoon ),( Yi Yang ),( Ho Bin Lee ),( Soo Nok Oh ),( Min Hye Jeong ),( Jong Jin Kim ),( Sung Tae Yee ),( Florin Crisan ),( Cheol Moon ),( Kwang Youl Lee ),( Kyung Keun Kim ) 전남대학교 약품개발연구소 2014 약품개발연구지 Vol.23 No.-
Lichens are symbiotic organisms which produce distinct secondary metabolic products. In the present study, we tested the cytotoxic activity of 17 lichen species against several human cancer cells and further investigated the molecular mechanisms underlying their anti-cancer activity. We found that among 17 lichens species, F. cucullata exhibited the most potent cytotoxicity in several human cancer cells. High performance liquid chromatography analysis revealed that the acetone extract of F. cucullata contains usnic acid, salazinic acid, Squamatic acid, Baeomycesic acid, d-protolichesterinic acid, and lichesterinic acid as subcomponents. MlT assay showed that cancer cell lines were more vulnerable to the cytotoxic effects of the extract than non-cancer cell lines. Furthermore, among the identified subcomponents, usnic acid treatment had a similar cytotoxic effect on cancer cell lines but with lower potency than the extract. At a lethal dose, treatment with the extract or with usnic acid greatly increased the apoptotic cell population and specifically activated the apoptotic signaling pathway; however, using sub-lethal doses, extract and usnic acid treatment decreased cancer cell motility and inhibited in vitro and in vivo tumorigenic potentials. In these cells, we observed significantly reduced levels of epithelialmesenchymal transition (EMn markers and phosphor-Akt, while phosphor-c-Jun and phosphor-ERK1/2 levels were only marginally affected. Overall, the anti-cancer activity of the extract is more potent than that of usnic acid alone. Taken together, F. cucullata and its subcomponent, usnic acid together with additional component, exert anti-cancer effects on human cancer cells through the induction of apoptosis and the inhibition of EMT.
오소미,전천후,박선주,장보형,박정수,장수빈,신용철,고성규,Oh, Somi,Cheon, Chunhoo,Park, Sunju,Jang, Bo-Hyoung,Park, Jeong-Su,Jang, Soobin,Shin, Yong-Cheol,Ko, Seong-Gyu 대한예방한의학회 2014 대한예방한의학회지 Vol.18 No.1
Objective : This study is aimed to analyze the recent research trend of Sipjeondabo-tang (SJDBT) in Korea. Method : We searched Korean research database by using keyword 'Sipjeondabo-tang'. Databases are Korean Medicine Information System, Research Information Service System and Korean Studies Information Service System. We analyzed studies by research method, publication year, type of journal and subject of the study. Results : By analyzing studies published after 1990, we could find that experimental studies were primarily conducted in SJDBT study. During recent 10 years, the number of toxicity studies and components analysis studies is increasing. Among the SJDBT studies, the number of clinical study was only 1. Conclusion : We explored the recent research trend of SJDBT in Korea. As a result, We suggested three further research ways of SJDBT. Proposed research ways are exploring various effect of SJDBT, observing whether there is a difference of effect according to extracting and drying method, and conducting clinical research of SJDBT.