비강 및 비인두에 발생한 도립유두종과 편평상피암종의 p53단백 및 세포증식능에 관한 연구

한주호,윤기중,이재규,신대균,박근호,조향정,문형배 圓光大學校 醫科學硏究所 1996 圓光醫科學 Vol.12 No.2

The inverted papilloma and squamous cell carcinoma are common neoplasia in the sinonasal cavity and nasopharynx, but the incidence of these tumors are very low and the study on the oncogenesis or biological activity of the tumor cells are not well known. This study was designed to evaluate the oncogenic roles of the p53 gene and the proliferative activity of the tumor cells in the inflammatory polyp, inverted papilloma and squamous cell carcinoma. The experiment was carried by the immunohistochemical stains about the p53 protein, PCNA and Ki-67, histochemical stain about the AgNORs. and flow cytometric analysis about the DNA ploidy using the formalin fixed paraffin embedded tissues. The frequency of the expression of p53 protein was 0%(0/16 cases) in the inflammatory polyps, 18.8% (3/16 cases) in the inverted papillomas, and 87.5%(14/16 cases) in the squamous cell carcinomas. The labelling index(%) of the PCNA and Ki-67 was 5.3% and 3.3% in the inflammatoy polyps, 29.6% and 25.2% in the inverted papillomas, and 51.9% and 36.8% in the squamous cell carcinoma. The expression of the PCNA and Ki-67 was distributed in the periphery of the tumor islands of the inverted papilloma and was distributed in the both of center and periphery of the tumor islands of the squamous cell carcinoma. The number of nuclear AgNORs was increased in the order of inflammatory polyps (0.96), inverted papillomas(1.34) and squamous cell carcinoma(2.09). The frequency of the DNA aneuploidy was 0%(0/16 cases) in the inflammatory polyps, 18.8%(3/16 cases) in the inverted papillomas. and 12.5%(2/16 cases) in the squamous cell carcinomas. Above results indicates that the changes of the p53 gene and proliferative activity of the tumor cells are involved on the oncogenesis and the biological activity of the inverted papilloma or squamous cell carcinoma in the nasopharynx and sinonasa cavity.

침전법에 의한 CaCO₃:Sn^(2+) 형광체의 합성

한상목,신대용,윤봉구 강원대학교 부설 석재복합신소재 제품연구센터 2004 석재연 논문집 Vol.9 No.-

침전법을 이용하여 CaCO₃를 합성한 후, 활성제로서 Sn^(2+)를 도핑하여 CaCO₃:Sn^(2+) 형광체를 합성하고 결정상과 형상 및 Sn^(2+) 첨가량에 따른 형광특성을 조사하였다. CaCl₂·2H₂O-(NH₄)₂CO₃수용액에 NH₄OH를 첨가하여 pH가 증가하면 입자의 응집이 관찰되지 않고 vaterite상이 장시간 안정하게 존재하였으며 pH 11에서는 1~3㎛의 순수한 구형 vaterite상이 얻어졌다. Vaterite인 구형 CaCO₃:Sn^(2+) 형광체와 열처리한 구형의 calcite형 CaCO₃:Sn^(2+) 형광체는 230 nm에서 최대로 여기되어 465 nm에서 최대 발광강도를 나타내었다. 합성된 CaCO₃:Sn^(2+) 형광체는 결정상과 관계없이 SnCl₂·2H₂O의 첨가량이 2 mol%일 때 가장 강한 발광강도를 나타내었으며, 능면체의 calcite형 CaCO₃:Sn^(2+)형광체는 열처리하여 안정화된 구형의 calcite CaCO₃:Sn^(2+) 형광체와 구형의 vaterite형 CaCO₃:Sn^(2+) 형광체에 비해 높은 발광강도를 나타내었다. Vaterite type of CaCO₃ was synthesized by using the precipitation method(CaCl₂-(NH₄)₂CO₃ system) with addition of NH₄OH at different temperature, time, pH and concentration. As a result, pure vaterite having spherical shape(0.1~10 ㎛ in size) was formed at the condition of high pH(>11) and high concentration(>5 M). CaCO₃:Sn^(2+) phosphors(spherical vaterite) were synthesized at CaCl₂-SnCl₂-(NH₄)₂CO₃-NH₄OH system. CaCO₃:Sn^(2+) phosphors(spherical vaterite) were transformed to CaCO₃:Sn^(2+) phosphors(spherical calcite) by heating at 550℃ for 10 min. The Particle sizes of CaCO₃:Sn^(2+) phosphors(rhombohedral calcite) and CaCO₃:Sn^(2+) phosphors (spherical vaterite) were 10~15 ㎛ and 1~3 ㎛ respectively. The excitation spectrum of CaCO₃:Sn^(2+) phosphors(spherical vaterite and spherical calcite) exhibited a maximum peak intensity at 230 nm with a narrow absorption band from 220 nm to 240 nm. At the excitation spectrum of 275nm, the emission spectrum of CaCO₃:Sn^(2+) phosphor (rhombohedral calcite) with SnCl₂of 2 mol% exhibited a maximum peak intensity at 465 nm of bright blue fluorescence. Also, the emission spectrum of CaCO₃:Sn^(2+) phosphors(spherical vaterite, spherical calcite) exhibited a maximum peak intensity at 465 nm but exhibited weak peak intensity than CaCO₃:Sn^(2+) phosphors(rhombohedral calcite).

청국장의 제조방법에 따른 향미 증진 효과

고한수,조대희,황성연,김영만 한국식품영양학회 1999 韓國食品營養學會誌 Vol.12 No.1

청국장 제조를 위한 분리균은 포자형성율이 높은 Bacillus subtilis로 대사력이 활발한 대수증식기의 것을 콩에 1,000CFU/g 이상의 균수를 접종하여 40℃와 상대습도 90%에서 배양하면 발효가 빠르게 진행되었다. 발효가 종료되면 포자형성율이 95% 이상 되어야 저온의 후숙과정에서 재번식이 쉽게 일어나지 않았으며 발효취는 발효 중에서만 형성되었다. 중립콩과 대립콩의 발효율이 가장 좋았다. 저온의 후숙과정은 5℃에서 1∼2일 후 경과하면 Bacillus subtilis는 더 이상의 증식이 일어나지 않고 발효취가 소멸되므로 저온의 유통과정을 거쳐야 불쾌취가 없는 구수한 청국장의 맛을 유지할 수 있었다. The strain isolated for making chungkuk-jang was Bacillus subtilis, which formed spore with 98% ratio. Logarithmical culture was inoculated (1,000 CFU /g) to the steamed soybeans and at the optimum fermentation conditions(40℃, RH 90%), fermentation progressed very rapidly and synchronously. Fermentation time was 24 hours on the optimum fermentation conditions. During activated fermentation, chungkuk-jang's aroma and flavor created. After finishing the fermentation, the spore forming ratio was 95% and replenishment was not occured easily during aging at the below 5℃.

다량의 하부위장관출혈이 병발된 Vibrio vulnificus 패혈증 1예

최주연,유진홍,한석원,이대훈,최민호,박순민,김연식,김선우,신완식,강문원,강성구 대한화학요법학회 1996 대한화학요법학회지 Vol.14 No.2

최근 저자들은 Vibrio vulnificus 감염에 의해 패혈증 및 이차성 피부 병변을 보인 42세의 남자 환자의 치료중 하부 위장관 출혈이 병발된 1예를 경험하였기에 보고하는 바이다. We experienced a case of Vibrop vulnificus septicemia which showed an unusual manifestation such as hematochezia. The patient was a 42-year-old man with chronic liver disease. The blood and wound culture revealed the organism. During intensive care, he suffered from massive hematochezi for several days. Colonoscopy and abdominal CT showed findings suspicious of ischemic colitis. To our knowledge, this is the first unusual case of V.vulnificus infection which showed massive lower gastrointestinal bleeding.

만성 승모판 패쇄부전증 환자에서의 안지오텐신 전환효소 억제제의 장기 투여 효과

김대희,이명묵,이해영,조현재,박승정,서재빈,서정원,양한모,윤창환,조상호,이준희,김용진,김명아,손대원,오병희,박영배 대한순환기학회 2004 Korean Circulation Journal Vol.34 No.2

철근 콘크리트 전단벽과 강재 보 전단접합부의 내진거동

이원호,윤현도,강대언,송한범,태경훈,박완신 대한건축학회 2004 대한건축학회 학술발표대회 논문집 - 계획계/구조계 Vol.24 No.1(구조계)

Ductile coupled flexural wall is the primary seismic load resisting system of buildings. In these systems, connections are generally headed stud bolt connection. The purpose of experimental study is to evaluate the seismic behavior of these connection under cyclic loading. A comprehensive experimental test involving 4 specimens has been performed and this program is used to study the effect of value of moment and edge distance. Through experimental test, the seismic behavior of typical headed stud bolt connections was established.

가족성 고콜레스테롤혈증 유병자에서 HMG Co-A Reductasen Inhibitor 및 Bile Acid Resin의 투여로 인한 아킬레스건 황색종의 감소

채인호,한기훈,박영배,조주희,김효수,손대원,김철호,오병희,이명묵,최윤식,서정돈,이영우 대한순환기학회 1997 Korean Circulation Journal Vol.27 No.9

전신성 색전증으로 발현된 감염성 심내막염 3례

김지수,박대균,박경창,홍경순,두영철,한규록,오동진,유규형,임종윤,고영박,이광학,이영 대한순환기학회 1999 Korean Circulation Journal Vol.29 No.8

HBx형질전환 생쥐에서 발생한 간세포암종에서 H-ras 및 c-myc의 발현에 관한 연구

문형배,소병준,김학철,윤기중,한원철,조향정,유대열,정영진 圓光大學校 醫科學硏究所 2002 圓光醫科學 Vol.17 No.2

<연구목적> HBx형질전환 생쥐에서 발생한 간세포암종의 발암과정에 종양유전자(H-rgs, c-myc)의 발현 정도를 조사하고자 하였다. <연구방법> 정상생쥐 12마리(4-18개월) 및 HBx 형질전환 생쥐 44마리(4-18개월)를 대상으로 포르말린에 고정하고 파라핀에 포매한 간 조직을 이용하여 면역조직화학적염색을 실시하였다. 실험군은 정상 부위, 이형성 부위 및 종양 부위로 구분하였으며, 종양 부위는 소결절성병변 부위와 간세 포암종 부위로 구분하였고, 이형성병변 부위는 이형성병변만 발견되는 부위, 소결절성병변과 동반된 이형성병변 부위 및 간세포암종과 동반된 이형성병변 부위로 구분하였다. <연구결과> H-rgs의 발현은 정상 간조직에 비하여 이형성병변 부위(P<0.05) 및 종양 부위(P<0.01)에서 증가하였으며, 소결절성병변 부위과 간세포암종 부위 사이에서는 간세포암종 부위에서 증가된 경향이었으나 통계학적으로 유의한 차이가 없었으며, 각 이형성병변 부위 사이에서도 유의한 차이는 없었다. c-myc의 발현은 정상 간조직 및 이형성병변 부위에 비해 종양 부위에서 증가하였으며(P<0.001), 소결절성병변 부위와 간세포암종 부위에서는 비슷하였고, 각 이형성병변부위 사이에서도 비슷하였다. <결론> HBx형질전환 생쥐에서 발생하는 간세포암종의 발생에 H-rgs는 이형성 변화를 일으키는 시기에 관여하며, c-myc은 이형성병변에서 암으로 이행하는 시기에 관여할 것으로 생각한다. Background: This experiment was designed for the expression of H-ras and c-myc in hepatocarcinogenesis of the HBx transgenic mice. Methods: Immunohistochemical stains in the paraffin embedded tissue of the liver were used for the detection of H-ras and c-myc in the 12 normal mice and 44 HBx transgenic mice of the 4-18 month old. Results: Expression of the H-ras was significantly increased in the dysplastic area (P<0.05) and tumor area (P<0.01) than in the normal liver. But there were no differences of H-ras expression between areas of microscopically identified hepatocellular carcinoma (MI-HCC) and grossly identified hepatocellular carcinoma (GI-HCC) and dysplastic areas among the only dysplastic areas, dysplastic areas with MI-HCC and GI-HCC. Expression of the c-myc was significantly increased in the tumor area (P<0.001) than in the normal liver and dysplastic area. But there were no differences of c-myc expression between areas of MI-HCC and GI-HCC, and dysplastic areas among only dysplastic areas, dysplastic areas with MI-HCC and GI-HCC. Conclusions: Our study suggests that H-ras is related to the dysplastic change and c-myc is related to the neoplastic change in the hepatocarcinogenesis of the HBx transgenic mice.

결핵성 육아종에서 Thioredoxin peroxidase-2 의 발현

박근호,유형륜,정영진,윤기중,한원철,유대열,문형배 圓光大學校 醫科學硏究所 1999 圓光醫科學 Vol.15 No.2

Background: Thioredoxin peroxidase(TPX) is a kind of recently discovered antioxidant enzyme which react as rapid hydrogen ion donor for the removal of hydroperoxide. The action and distribution of the TPX was poorly understood in the human diseases. This experiments were designed for the study about the distribution of the TPX in the chronic granulomatous inflammation and about the correlation between the expression of TPX and the site of inflammation, histological activities of tuberculous inflammation or existence of mycobacterium in the inflammatory foci. Methods: The immunohistochemical stains were performed for the localization of the TPX-2 in the epithelioid cells, giant cells and lymphocytes in the chronic granulomatous inflammation. The tissue sections were obtained from the paraffin blocks of the 54 cases of tuberculosis (lung 21 cases, lymph node 12 cases, bone and soft tissue 12 cases, kidney 9 cases; active 33 cases, inactive 21 cases by the histologic classification; presence of mycobacterium 15 cases, no mycobacterium 39 cases by PCR reaction). Results: The expression of TPX-2 was 16.7% in the giant cells, 27.8% in the epithelioid cells and 100% in the lymphocytes of tuberculous inflammations. The expression of TPX-2 in the giant cells and epithelioid cells of the tuberculosis were 28.6% and 57.1% of the pulmonary tuberculosis; 33.3% in each cells of the renal tuberculosis; 0% in each cells of the lymph node or bone and soft tissue tuberculosis. The expression of TPX-2 in the giant cells and epithelioid cells were 9.1% in each cells of the active tuberculosis and were 28.6% and 57.1% in each cells of the inactive tuberculosis by histologic classification. The expression of TPX-2 in the giant cells and epithelioid cells was 40% in each cells of tuberculosis which mycobacteria were detected and the expression of TPX-2 was 7.7% and 23.1% in each cells which mycobacteria were not detected by PCR reaction in the paraffin embedded tissue. Conclusions: The above results were summarized that the TPX-2 in the giant cells and epithelioid cells were more frequently expressed in the inactive tuberculosis than in the active tuberculosis. These results suggest that the TPX-2 is a kind of regulating or suppressing factors in the activity of the tuberculosis.