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Zhiguang Liu,Erxia Yang,Suhua Liu 한국멀티미디어학회 2006 한국멀티미디어학회 국제학술대회 Vol.2006 No.-
Based on the technology of Flash and ActionScript2.0 program, the paper discusses research methods in construction of virtual chemistry laboratories for comprehensive experiment. Through comparing with the three kinds of construction methods, the best method, which is the netted type to construct a virtual chemistry laboratory of comprehensive experiment, is selected. The comprehensive experiment is complex, and needs several laboratories to complete. When constructing a virtual laboratory of comprehensive experiment with general method, document produced by it is big and short of intelligence. The netted type exactly solves the problem well. It divides the big software into several small softwares, and each independent software is related with each other through exterior data transmission, forming an integrated onstage software commonly. Then it is related with the backstage database through PHP, JSP or ASP and forms a virtual laboratory of comprehensive experiment based on the network. The VCL constructed from the netted type is advantageous in running in the network, because it is small and the speed carrying out is quick. Users not only could be familiar with the apparatus but also master the processes after finishing experiments in the VCL, since the scene is reality, and the operation is flexible and believable. The goal is to provide online access to a remote laboratory for classes and deliver the educational benefits of hands-on experimentation to students anywhere, at any time, and the user can hover willfully in the virtual chemistry laboratory.
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You, Jiangfeng,Liu, Xing,Zhang, Bo,Xie, Zhongkai,Hou, Zhiguang,Yang, Zhenming The Korean Society of Ginseng 2015 Journal of Ginseng Research Vol.39 No.1
Background: In Changbai Mountains, Panax ginseng (ginseng) was cultivated in a mixture of the humus and albic horizons of albic luvisol in a raised garden with plastic shade. This study aimed to evaluate the impact of ginseng planting on soil characteristics. Methods: The mixed-bed soils were seasonally collected at intervals of 0-5 cm, 5-10 cm, and 10-15 cm for different-aged ginsengs. Soil physico-chemical characteristics were studied using general methods. Aluminum was extracted from the soil solids with $NH_4Cl $(exchangeable Al) and Na-pyrophosphate (organic Al) and was measured with an atomic absorption spectrophotometer. Results: A remarkable decrease in the pH, concentrations of exchangeable calcium, $NH_4^+$, total organic carbon (TOC), and organic Al, as well as a pronounced increase in the bulk density were observed in the different-aged ginseng soils from one spring to the next. The decrease in pH in the ginseng soils was positively correlated with the $NH_4^+$ (r=0.463, p<0.01), exchangeable calcium (r=0.325, p<0.01) and TOC (r= 0.292, p < 0.05) concentrations. The $NO_3^-$ showed remarkable surface accumulation (0-5 cm) in the summer and even more in the autumn but declined considerably the next spring. The exchangeable Al fluctuated from $0.10mg\;g^{-1}$ to $0.50mg\;g^{-1}$ for dry soils, which was positively correlated with the $NO_3^-$ (r=0.401, p < 0.01) and negatively correlated with the TOC (r=-0.329, p < 0.05). The Al saturation varied from 10% to 41% and was higher in the summer and autumn, especially in the 0-5 cmand 5-10 cm layers. Conclusion: Taken together, our study revealed a seasonal shift in soil characteristics in ginseng beds with plastic shade.
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Jiangfeng You,Xing Liu,Bo Zhang,Zhongkai Xie,Zhiguang Hou,Zhenming Yang 고려인삼학회 2015 Journal of Ginseng Research Vol.39 No.3
Background: In Changbai Mountains, Panax ginseng (ginseng) was cultivated in a mixture of the humus and albic horizons of albic luvisol in a raised garden with plastic shade. This study aimed to evaluate the impact of ginseng planting on soil characteristics. Methods: The mixed-bed soils were seasonally collected at intervals of 0-5 cm, 5-10 cm, and 10-15 cm for different-aged ginsengs. Soil physico-chemical characteristics were studied using general methods. Aluminum was extracted from the soil solids with NH₄Cl (exchangeable Al) and Na-pyrophosphate (organic Al) and was measured with an atomic absorption spectrophotometer. Results: A remarkable decrease in the pH, concentrations of exchangeable calcium, NH₄⁺, total organic carbon (TOC), and organic Al, as well as a pronounced increase in the bulk density were observed in the different-aged ginseng soils from one spring to the next. The decrease in pH in the ginseng soils was positively correlated with the NH⁺₄ (r=0.463, p<0.01), exchangeable calcium (r=0.325, p<0.01) and TOC (r = 0.292, p < 0.05) concentrations. The NO₃ showed remarkable surface accumulation (0-5 cm) in the summer and even more in the autumn but declined considerably the next spring. The exchangeable Al fluctuated from 0.10 mg g<SUP>-1</SUP> to 0.50 mg g<SUP>-1</SUP> for dry soils, which was positively correlated with the NO₃ (r=0.401, p < 0.01) and negatively correlated with the TOC (r=-0.329, p < 0.05). The Al saturation varied from 10% to 41% and was higher in the summer and autumn, especially in the 0-5 cmand 5-10 cm layers. Conclusion: Taken together, our study revealed a seasonal shift in soil characteristics in ginseng beds with plastic shade.
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A Stealthy Attack Against Tor Guard Selection
Quangang Li,Peipeng Liu,Zhiguang Qin 보안공학연구지원센터 2015 International Journal of Security and Its Applicat Vol.9 No.11
Tor is a popular low-latency anonymous communication system which could provide anonymity and anti-censorship. Based on previous researches on de- anonymization of Tor, this paper proposes a novel approach to attack users’ guard selection which can pose great threat against Tor users' anonymity. Under the current design of Tor, once entry guards are compromised, the probability that an attacker observes both ends of a Tor circuit will be highly improved. Actual and simulated experiments both show that an attacker (e.g., a local or national government which have the power to monitor a Tor user’s internet connection) can successfully compromise a specific Tor user’s entry guard in about 30 minutes, and this can further help de-anonymize the user’s anonymous communication.
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Jiangfeng You,Xing Liu,Bo Zhang,Zhongkai Xie,Zhiguang Hou,Zhenming Yang 고려인삼학회 2015 Journal of Ginseng Research Vol.39 No.1
Background: In Changbai Mountains, Panax ginseng (ginseng) was cultivated in a mixture of the humusand albic horizons of albic luvisol in a raised garden with plastic shade. This study aimed to evaluate theimpact of ginseng planting on soil characteristics. Methods: The mixed-bed soils were seasonally collected at intervals of 0e5 cm, 5e10 cm, and 10e15 cmfor different-aged ginsengs. Soil physico-chemical characteristics were studied using general methods. Aluminum was extracted from the soil solids with NH4Cl (exchangeable Al) and Na-pyrophosphate(organic Al) and was measured with an atomic absorption spectrophotometer. Results: A remarkable decrease in the pH, concentrations of exchangeable calcium, NH4þ, total organiccarbon (TOC), and organic Al, as well as a pronounced increase in the bulk density were observed in thedifferent-aged ginseng soils from one spring to the next. The decrease in pH in the ginseng soils waspositively correlated with the NHþ4 (r¼0.463, p<0.01), exchangeable calcium (r¼0.325, p<0.01) and TOC(r ¼ 0.292, p < 0.05) concentrations. The NO3 showed remarkable surface accumulation (0e5 cm) in thesummer and even more in the autumn but declined considerably the next spring. The exchangeable Alfluctuated from 0.10 mg g1 to 0.50 mg g1 for dry soils, which was positively correlated with the NO3(r ¼ 0.401, p < 0.01) and negatively correlated with the TOC (r¼0.329, p < 0.05). The Al saturation variedfrom 10% to 41% and was higher in the summer and autumn, especially in the 0e5 cmand 5e10 cm layers. Conclusion: Taken together, our study revealed a seasonal shift in soil characteristics in ginseng bedswith plastic shade.
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Yuxiao Zhu,Li Qian,Qing Liu,Jing Zou,Ying Zhou,Tao Yang,Gan Huang,Zhiguang Zhou,Yu Liu 대한당뇨병학회 2020 Diabetes and Metabolism Journal Vol.44 No.2
Background: The detection of glutamic acid decarboxylase 65 (GAD65) autoantibodies is essential for the prediction and diagnosis of latent autoimmune diabetes in adults (LADA). The aim of the current study was to compare a newly developed electrochemiluminescence (ECL)-GAD65 antibody assay with the established radiobinding assay, and to explore whether the new assay could be used to define LADA more precisely. Methods: Serum samples were harvested from 141 patients with LADA, 95 with type 1 diabetes mellitus, and 99 with type 2 diabetes mellitus, and tested for GAD65 autoantibodies using both the radiobinding assay and ECL assay. A glutamic acid decarboxylase antibodies (GADA) competition assay was also performed to assess antibody affinity. Furthermore, the clinical features of these patients were compared. Results: Eighty-eight out of 141 serum samples (62.4%) from LADA patients were GAD65 antibody-positive by ECL assay. Compared with ECL-GAD65 antibody-negative patients, ECL-GAD65 antibody-positive patients were leaner (P<0.0001), had poorer β-cell function (P<0.05), and were more likely to have other diabetes-associated autoantibodies. The β-cell function of ECLGAD65 antibody-positive patients was similar to that of type 1 diabetes mellitus patients, whereas ECL-GAD65 antibody-negative patients were more similar to type 2 diabetes mellitus patients. Conclusion: Patients with ECL-GAD65 antibody-negative share a similar phenotype with type 2 diabetes mellitus patients, whereas patients with ECL-GAD65 antibody-positive resemble those with type 1 diabetes mellitus. Thus, the detection of GADA using ECL may help to identify the subtype of LADA.
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우기도,김재황,권의표,문민석,이현범,Tatsuo Sato,Zhiguang Liu 대한금속·재료학회 2010 METALS AND MATERIALS International Vol.16 No.2
To fabricate an Al-V matrix composite reinforced with submicron-sized Al2O3 and AlxVy (Al3V, Al10V) phases,high energy mechanical milling (HEMM) and sintering were employed. By increasing the milling time, the size of mechanically milled powder was significantly reduced. In this study, the average powder size of 59 μm for Al, and 178 μm for V2O5 decreased with the formation of a new product, Al-Al2O3-AlxVy, with a size range from 1.3 μm to 2.6 μm formed by the in-situ combustion reaction during sintering of HEM milled Al and V2O5 composite powders. The in-situ reaction between Al and V2O5 during the HEMM and sintering transformed the Al2O3 and AlxVy (Al3V, Al10V) phases. Most of the reduced V reacted with excess the Al to form AlxVy (Al3V, Al10V) with very little V dissolved into Al matrix. By increasing the milling time and weight percentage of V2O5, the hardness of the Al-Al2O3-AlxVy composite sintered at 1173 K increased. The composite fabricated with the HEMM Al-20wt.%V2O5 composite powder and sintering at 1173 K for 2 h had the highest hardness.
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Yujia Shi,Xingli Fu,Qi Cao,Zhengdao Mao,Yi Chen,Yun Sun,Zhiguang Liu,Qian Zhang 대한천식알레르기학회 2018 Allergy, Asthma & Immunology Research Vol.10 No.3
Purpose: Molecular mechanisms leading to asthma is still ill-defined. Though the function of microRNAs (miRNAs) in asthma was previously reported, the involvement of miR-155 in important features of this disease remains unknown. The present study was designed to uncover the probable involvement of miR-155-5p in the proliferation and migration of IL-13-induced human bronchial smooth muscle cells (BSMCs) and the intrinsic regulatory mechanism. Methods: The effects of different concentrations of IL-13 on the proliferation and migration of BSMCs as well as the expression of miR-155-5p and its predicted target transforming growth factor (TGF)-β-activated kinase 1/MAP3K7-binding protein 2 (TAB2) were investigated. The effects of miR-155-5p on the proliferation and migration of interleukin (IL)-13-induced BSMCs was determined in vitro using BSMCs transfected with miR-155 mimic/inhibitor and induced by a high concentration of IL-13. The quantitative real-time polymerase chain reaction (qRT-PCR) was employed for determining the expression of miR-155-5p and TAB2. Western blotting was applied to analyze the expression of TAB2 at the protein level. Cell proliferation and migration were assessed using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and Transwell assays, respectively. Results: The proliferation and migration of BSMCs were dose-dependently increased with IL-13 treatment. Contrariwise, IL-13 dose-dependently inhibited the expression of miR-155-5p in BSMCs. Mechanistic studies showed that inhibition of miR-155-5p further promoted the stimulatory effects of IL-13, whereas overexpression of miR-155 significantly inhibited these effects. In silico studies and luciferase reporter assays indicated that TAB2 was a negatively regulated miR-155-5p target. Conclusions: These results suggested that miR-155-5p-inhibit the IL-13-induced proliferation and migration of BSMCs by targeting TAB2 and that the IL-13/miR-155/TAB2 pathway could serve as a therapeutic target for pulmonary diseases, especially asthma.
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