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Xing-Jia Shen,Yong-Zhu Yi,Shun-Ming Tang,Zhi-Fang Zhang,Yi-Ren Li,Jia-Lu He,Xiang-Fu Wu 한국잠사학회 2004 International Journal of Industrial Entomology Vol.8 No.2
Bombyx mori nucleopolyhedrovirus (BmNPV) ecdysteroid UDP-glucosyltransferase gene (egt) promoter fragments of different lengths were amplified from BmNPV ZJ-8 genomic DNA by PCR. Reporter plasmids pBmegt542-luc, pBmegt309-luc and pBmegt159- luc with luciferase (luc) driven by egt promoters were constructed. Both in vitro and in vivo expressions showed that BmNPV egt promoter activity requires the transactivation of viral factor(s), and expression of luc was detected earliest at 24 hrs post infection (pi). BmNPV ZJ-8 homologous region 3 (hr3) increased the expression of luc by over 1,600-fold. Molting hormone of 1.0 ~ 2.0 mg/ml can dramatically down regulate expression of luc. Juvenile hormone analogue of 0.5 ~ 2.0 mg/ml increased expression of luc by 145.8% to 75.7%. Deletion assay revealed that the promoter fragment of 159 bp contains the basal promoter structure; Promoter fragments of 309 bp and 542 bp showed similar but much higher transcriptional activities than that of 159 bp, suggesting that nucleotide from -159 to -309 nt upstream the translation initiation site harbors the main cis-acting elements.
Inhibitory Lignans against NFAT Transcription Factor from Acanthopanax koreanum
Xing Fu Cai,Im Seon Lee,Nguyen Tien Dat,Guanghai Shen,강종성,Dong Hyun Kim,Young Ho Kim 대한약학회 2004 Archives of Pharmacal Research Vol.27 No.7
Three lignans isolated from the roots of A. koreanum (Araliaceae), namely eleutheroside E (1), tortoside A (2), and hemiariensin (4), were evaluated for their ability to inhibit NFAT transcription factor. Of these compounds, compound 4, possessing a diarylbutane skeleton, exhibited potent inhibitory activity against NFAT transcription factor (IC50: 36.3 ± 2.5 mM). However, the activities of 1 (IC50: > 500 mM) and 2 (IC50: 136.1 ± 9.4 mM), which possess bisaryldioxabicyclooctane skeletons, were lower. As the lignan derivatives of the same skeletons, hinokinin (5) and (-)-yatein (6) with diarylbutane skeletons and (+)-syringaresinol (3) with a bisaryldioxabicyclooctane skeleton were also studied for their inhibitory effects on NFAT transcription factor.
Fu Hanguang,Song Xuding,Lei Yongping,Jiang Zhiqiang,Xing Jiandong,Yang Jun,Wang Jinhua 대한금속·재료학회 2009 METALS AND MATERIALS International Vol.15 No.3
The effects of quenching treatment on the microstructure, hardness, impact toughness, and wear resistance of low-carbon high-boron cast steel (LCHBS) containing 0.15-0.3 %C, 1.4-1.8 %B, 0.3-0.8 %Si, 0.8-1.2 %Mn, 0.5-0.8%Cr, 0.3-0.6%Ni, and 0.3-0.6%Mo have been investigated by optical microscopy (OM), scanning electron microscopy (SEM), and transmission electron microscopy (TEM), and via an electron probe microanalyzer (EPMA), X-ray diffraction (XRD) analysis, impact tester, hardness tester, and wear tester. The as-cast matrix of LCHBS consists of pearlite and ferrite. There is 8-10 vol.% Fe2(B, C) type borocarbides in the matrix. The micro-hardness of Fe2(B, C) is 1430-1480 Hv. Fe2(B,C) shows no obvious change and the matrix completely transforms into lath martensite upon quenching at 900 °C to 1100 °C. The microhardness of the matrix and the macrohardness of the LCHBS sample show a slight increase with an increase of homogenization temperature. When the homogenization temperature exceeds 1050 °C, no distinct change in the hardness is observed. The change of homogenization temperature has no apparent effect on the impact toughness of LCHBS. The mass losses of LCHBS increase distinctly when the wear load increases. The homogenization temperature is less than 1000 °C and the wear rate of LCHBS decreases with an increase of temperature. The wear rate does not display any obvious change after exceeding a homogenization temperature of 1000 °C. The effects of quenching treatment on the microstructure, hardness, impact toughness, and wear resistance of low-carbon high-boron cast steel (LCHBS) containing 0.15-0.3 %C, 1.4-1.8 %B, 0.3-0.8 %Si, 0.8-1.2 %Mn, 0.5-0.8%Cr, 0.3-0.6%Ni, and 0.3-0.6%Mo have been investigated by optical microscopy (OM), scanning electron microscopy (SEM), and transmission electron microscopy (TEM), and via an electron probe microanalyzer (EPMA), X-ray diffraction (XRD) analysis, impact tester, hardness tester, and wear tester. The as-cast matrix of LCHBS consists of pearlite and ferrite. There is 8-10 vol.% Fe2(B, C) type borocarbides in the matrix. The micro-hardness of Fe2(B, C) is 1430-1480 Hv. Fe2(B,C) shows no obvious change and the matrix completely transforms into lath martensite upon quenching at 900 °C to 1100 °C. The microhardness of the matrix and the macrohardness of the LCHBS sample show a slight increase with an increase of homogenization temperature. When the homogenization temperature exceeds 1050 °C, no distinct change in the hardness is observed. The change of homogenization temperature has no apparent effect on the impact toughness of LCHBS. The mass losses of LCHBS increase distinctly when the wear load increases. The homogenization temperature is less than 1000 °C and the wear rate of LCHBS decreases with an increase of temperature. The wear rate does not display any obvious change after exceeding a homogenization temperature of 1000 °C.
( Xing Jia Shen ),( Yong Zhu Yi ),( Shun Ming Tang ),( Zhi Fang Zhang ),( Yi Ren Li ),( Jia Lu He ),( Xiang Fu Wu ) 한국잠사학회 2007 International Journal of Industrial Entomology Vol.8 No.2
Bombyx mori nucleopolyhedrovirus(BmNPV) ecdysteroid UDP-glucosyltransferase gene (egt) promoter fragments of different lengths were amplified from BmNPV ZJ-8 genomic DNA by PCR. Reporter plasmids pBmegt542-luc, pBmegt309-luc and pBmegtl59-luc with luciferase (luc) driven by egt promoters were constructed. Both in vitro and in vivo expressions showed that BmNPV egt promoter activity requires the transactivation of viral factor(s), and expression of luc was detected earliest at 24 hrs post infection (pi). BmNPV ZJ-8 homologous region 3 (hr3) increased the expression of luc by over 1,600-fold. Molting hormone of 1.0∼2.0㎍/㎖ can dramatically down regulate expression of luc. Juvenile hormone analogue of 0.5∼2.0㎍/㎖ increased expression of luc by 145.8% to 75.7%. Deletion assay revealed that the promoter fragment of 159 bp contains the basal promoter structure; Promoter fragments of 309 bp and 542 bp showed similar but much higher transcriptional activities than that of 159 bp, suggesting that nucleotide from -159 to -309 nt upstream the translation initiation site harbors the main cis-acting elements.
A high-efficiency simulation method of wind field and its application on transmission line
Xing Fu,Xing-Heng Zhang,Hong-Nan Li,Gang Li,Hui-Juan Liu 한국풍공학회 2021 Wind and Structures, An International Journal (WAS Vol.33 No.4
Generally, the fluctuating wind is simplified as several independent one-dimensional multivariate stationary Gaussian processes in simulating a natural wind field. The correlation in the lateral, longitudinal and vertical directions should all be considered in the simulation of longitudinal wind field for the large-span spatial structures. In fact, this type of structure has lots of simulation points. The calculation amount of wind field simulation by the harmonic superposition method depends on the scale of cross-spectral density matrix, which is directly related to the number of simulated points, leading to a low efficiency when generating the time-varying wind speed. This paper innovatively proposes a high-efficiency simulation method for the longitudinal wind field based on Taylor’s hypothesis. Subsequently, the effectiveness of the proposed wind field method was verified by the numerical simulation. Finally, the dynamic responses of a transmission tower-line system under the wind loadings generated with the new method and traditional method are calculated and compared. The percentages difference of the mean and maximum axial force at the main tower members are less than 0.02% and 1%, respectively, indicating the effectiveness of the proposed time delay method. The results also show that the proposed simulation method of wind field can not only ensure the simulation accuracy, but also significantly improve the efficiency of wind speed generation, which is suitable for the wind load simulation of large-span spatial structures.
Fu-Bao Zheng,Chang-Wen Zhang,Pei-Ji Wang,Hang-Xing Luan 한국물리학회 2013 Current Applied Physics Vol.13 No.4
Using full-potential linearized augmented plane wave (FLAPW) method, we investigated the effects of intrinsic vacancies on electronic and magnetic properties in graphene-like ZnO nanosheets. The results show that the oxygen vacancy (VO) has no influence on magnetism in ZnO nanosheet, whereas the Zn vacancy (VZn) lead to spin polarization of the nanostructures with a total magnetic moment of 2.0mB due to O-2p and Zn-3d hybridization. When the distance of two VZn defects increases to 6.499 A, the system shows an intriguing half-metallic character with 100% spin-polarized carriers due to O(2p)-Zn(3d)-O(2p) coupling chain between two VZn defects.