Effect of Chemical Admixtures on Setting Time, Fluidity and Mechanical Properties of Phosphorus Gypsum Based Self-Leveling Mortar

Zhenzhen Zhi,Jian Huang,Yanfei Guo,Siwen Lu,Baoguo Ma 대한토목학회 2017 KSCE JOURNAL OF CIVIL ENGINEERING Vol.21 No.5

Phosphorus Gypsum (PG) based Self-Leveling mortar (GSL) is a green and efficient material for floor construction. Its hydration kinetics, rheology property and their effect on mechanical strength of hydrated GSL were crucial to its final application. This study analyzed the effect on setting time of three retarders, namely Protein Salt (PS), Citric Acid (CA), and Sodium Tripolyphosphate (STPP). The fluidity of GSL was investigated individually using three different types of superplasticizer, namely naphthalene (FDN), polycarboxylate (PCE) and melamine (MSF). Finally, the effect of these chemical admixtures on mechanical properties of GSL hardened was examined. Results showed that the optimal contents of PS were 0.1%-0.15% by weight. With 0.1% PS, setting time of GSL retarded to 80min. To reach a maximum fluidity of GSL to 350mm, the optimal contents of PCE, MSF and FDN were 0.2%, 0.6% and 1.0% respectively and corresponding compressive strength of GSL were 54.25MPa, 53MPa and 52.25MPa. Suggesting PCE is the most effective dispersant in PG based GSL system.

Knockdown of Pyruvate Kinase M Inhibits Cell Growth and Migration by Reducing NF-κB Activity in Triple-Negative Breast Cancer Cells

Ma, Chaobing,Zu, Xueyin,Liu, Kangdong,Bode, Ann M.,Dong, Zigang,Liu, Zhenzhen,Kim, Dong Joon Korean Society for Molecular and Cellular Biology 2019 Molecules and cells Vol.42 No.9

Altered genetic features in cancer cells lead to a high rate of aerobic glycolysis and metabolic reprogramming that is essential for increased cancer cell viability and rapid proliferation. Pyruvate kinase muscle (PKM) is a rate-limiting enzyme in the final step of glycolysis. Herein, we report that PKM is a potential therapeutic target in triple-negative breast cancer (TNBC) cells. We found that PKM1 or PKM2 is highly expressed in TNBC tissues or cells. Knockdown of PKM significantly suppressed cell proliferation and migration, and strongly reduced S phase and induced G2 phase cell cycle arrest by reducing phosphorylation of the CDC2 protein in TNBC cells. Additionally, knockdown of PKM significantly suppressed $NF-{\kappa}B$ (nuclear factor kappa-light-chain-enhancer of activated B cells) activity by reducing the phosphorylation of p65 at serine 536, and also decreased the expression of $NF-{\kappa}B$ target genes. Taken together, PKM is a potential target that may have therapeutic implications for TNBC cells.

Knockdown of Pyruvate Kinase M Inhibits Cell Growth and Migration by Reducing NF-kB Activity in Triple-Negative Breast Cancer Cells

Chaobing Ma,Xueyin Zu,Kangdong Liu,Ann M. Bode,지강동,Zhenzhen Liu,김동준 한국분자세포생물학회 2019 Molecules and cells Vol.42 No.9

Altered genetic features in cancer cells lead to a high rate of aerobic glycolysis and metabolic reprogramming that is essential for increased cancer cell viability and rapid proliferation. Pyruvate kinase muscle (PKM) is a rate-limiting enzyme in the final step of glycolysis. Herein, we report that PKM is a potential therapeutic target in triple-negative breast cancer (TNBC) cells. We found that PKM1 or PKM2 is highly expressed in TNBC tissues or cells. Knockdown of PKM significantly suppressed cell proliferation and migration, and strongly reduced S phase and induced G2 phase cell cycle arrest by reducing phosphorylation of the CDC2 protein in TNBC cells. Additionally, knockdown of PKM significantly suppressed NF-kB (nuclear factor kappa-light-chain-enhancer of activated B cells) activity by reducing the phosphorylation of p65 at serine 536, and also decreased the expression of NF-kB target genes. Taken together, PKM is a potential target that may have therapeutic implications for TNBC cells.

Prognostic Value of the Evolution of HER2-Low Expression after Neoadjuvant Chemotherapy

Youzhao Ma,Mingda Zhu,Jingyang Zhang,Minhao Lv,Xiuchun Chen,Zhenzhen Liu 대한암학회 2023 Cancer Research and Treatment Vol.55 No.4

Purpose Patients with human epidermal growth factor receptor 2 (HER2)–low advanced breast cancer can benefit from trastuzumab deruxtecan. Given the unclear prognostic characteristics of HER2-low breast cancer, we investigated the prognostic characteristics of HER2-low expression from primary tumor to residual disease after neoadjuvant chemotherapy (NACT). Materials and Methods The data of HER2-negative patients receiving NACT at our center were collected. Pathological complete response (pCR) rate were compared between HER2-0 and HER2-low patients. The evolution of HER2 expression from primary tumor to residual disease and its impact on disease-free survival (DFS) were examined. Results Of the 690 patients, 494 patients had HER2-low status, of which 72.3% were hormone receptor (HR)–positive (p < 0.001). The pCR rates of HER2-low and HER2-0 patients (14.2% vs. 23.0%) showed no difference in multivariate analysis regardless of HR status. No association was observed between DFS and HER2 status. Of the 564 non-pCR patients, 57 (10.1%) changed to HER2-positive, and 64 of the 150 patients (42.7%) with HER2-0 tumors changed to HER2-low. HER2-low (p=0.004) and HR-positive (p=0.010) tumors before NACT were prone to HER2 gain. HER2 gain patients had a better DFS compared with HER2-negative maintained patients (87.9% vs. 79.5%, p=0.048), and the DFS of targeted therapy group was better than that of no targeted therapy group (92.4% vs. 66.7%, p=0.016). Conclusion Although HER2-low did not affect the pCR rate and DFS, significant evolution of HER2-low expression after NACT creates opportunities for targeted therapy including trastuzumab.

Effects of Dietary Lycopene Supplementation on Plasma Lipid Profile, Lipid Peroxidation and Antioxidant Defense System in Feedlot Bamei Lamb

Hongqin Jiang,Zhenzhen Wang,Yong Ma,Yanghua Qu,Xiao Nan Lu,Hailing Luo 아세아·태평양축산학회 2015 Animal Bioscience Vol.28 No.7

Lycopene, a red non-provitamin A carotenoid, mainly presenting in tomato and tomato byproducts, has the highest antioxidant activity among carotenoids because of its high number of conjugated double bonds. The objective of this study was to investigate the effect of lycopene supplementation in the diet on plasma lipid profile, lipid peroxidation and antioxidant defense system in feedlot lamb. Twenty-eight Bamei male lambs (90 days old) were divided into four groups and fed a basal diet (LP0, 40:60 roughage: concentrate) or the basal diet supplemented with 50, 100, and 200 mg/kg lycopene. After 120 days of feeding, all lambs were slaughtered and sampled. Dietary lycopene supplementation significantly reduced the levels of plasma total cholesterol (p<0.05, linearly), total triglycerides (TG, p<0.05) and low-density lipoprotein cholesterol (LDL-C, p<0.05), as well as atherogenic index (p<0.001), whereas no change was observed in high-density lipoprotein cholesterol (p>0.05). The levels of TG (p<0.001) and LDL-C (p<0.001) were decreased with the feeding time extension, and both showed a linear trend (p<0.01). Malondialdehyde level in plasma and liver decreased linearly with the increase of lycopene inclusion levels (p<0.01). Dietary lycopene intake linearly increased the plasma antioxidant vitamin E level (p<0.001), total antioxidant capacity (T-AOC, p<0.05), and activities of catalase (CAT, p<0.01), glutathione peroxidase (GSH-Px, p<0.05) and superoxide dismutase (SOD, p<0.05). The plasma T-AOC and activities of GSH-Px and SOD decreased with the extension of the feeding time. In liver, dietary lycopene inclusion showed similar antioxidant effects with respect to activities of CAT (p<0.05, linearly) and SOD (p<0.001, linearly). Therefore, it was concluded that lycopene supplementation improved the antioxidant status of the lamb and optimized the plasma lipid profile, the dosage of 200 mg lycopene/kg feed might be desirable for growing lambs to prevent environment stress and maintain normal physiological metabolism.

Genetic and antigenic characterization of fragments of the pheasant immunoglobulin Y heavy chain constant region

Jing Zhai,Weishan Chang,Zhenzhen Zhai,Xingliang Ma,Lingling Yang,Jinfeng Ti,Daozhen Song,Shijun Fu,Dapeng Li 한국유전학회 2016 Genes & Genomics Vol.38 No.8

Few studies have attempted to characterize the pheasant (Phasianus colchicus) immunoglobulin Y (IgY) heavy chain constant region. In the present study, fragments of the pheasant IgY heavy chain constant region were cloned, analyzed, and expressed. The cross-reactivity of IgY or immunoglobulin G (IgG)s with antigens from other vertebrate species was determined using dot-enzymelinked immunosorbent assay and western blot analysis. Five peptides of the pheasant IgY heavy chain constant region were synthesized to determine its immunoregulatory activity in vitro. The IgY heavy chain constant region from pheasant showed the highest homology with that from chicken (71.2 %) and duck (49.1 %). Phylogenetic analysis for IgY showed that pheasant was closely related to chicken and duck than to any other analyzed vertebrate species. The rabbit anti-chicken IgG showed immunologic cross-reactivity with recombinant proteins of the pheasant IgY heavy chain constant region. Four peptides were able to induce significant up-regulation of interleukin (IL)-1b, IL-4, and interferon-c in chicken peripheral blood lymphocytes, suggesting a new role of avian IgY in immune regulation.

Effects of Dietary Lycopene Supplementation on Plasma Lipid Profile, Lipid Peroxidation and Antioxidant Defense System in Feedlot Bamei Lamb

Jiang, Hongqin,Wang, Zhenzhen,Ma, Yong,Qu, Yanghua,Lu, Xiaonan,Luo, Hailing Asian Australasian Association of Animal Productio 2015 Animal Bioscience Vol.28 No.7

Lycopene, a red non-provitamin A carotenoid, mainly presenting in tomato and tomato byproducts, has the highest antioxidant activity among carotenoids because of its high number of conjugated double bonds. The objective of this study was to investigate the effect of lycopene supplementation in the diet on plasma lipid profile, lipid peroxidation and antioxidant defense system in feedlot lamb. Twenty-eight Bamei male lambs (90 days old) were divided into four groups and fed a basal diet (LP0, 40:60 roughage: concentrate) or the basal diet supplemented with 50, 100, and 200 mg/kg lycopene. After 120 days of feeding, all lambs were slaughtered and sampled. Dietary lycopene supplementation significantly reduced the levels of plasma total cholesterol (p<0.05, linearly), total triglycerides (TG, p<0.05) and low-density lipoprotein cholesterol (LDL-C, p<0.05), as well as atherogenic index (p<0.001), whereas no change was observed in high-density lipoprotein cholesterol (p>0.05). The levels of TG (p<0.001) and LDL-C (p<0.001) were decreased with the feeding time extension, and both showed a linear trend (p<0.01). Malondialdehyde level in plasma and liver decreased linearly with the increase of lycopene inclusion levels (p<0.01). Dietary lycopene intake linearly increased the plasma antioxidant vitamin E level (p<0.001), total antioxidant capacity (T-AOC, p<0.05), and activities of catalase (CAT, p<0.01), glutathione peroxidase (GSH-Px, p<0.05) and superoxide dismutase (SOD, p<0.05). The plasma T-AOC and activities of GSH-Px and SOD decreased with the extension of the feeding time. In liver, dietary lycopene inclusion showed similar antioxidant effects with respect to activities of CAT (p<0.05, linearly) and SOD (p<0.001, linearly). Therefore, it was concluded that lycopene supplementation improved the antioxidant status of the lamb and optimized the plasma lipid profile, the dosage of 200 mg lycopene/kg feed might be desirable for growing lambs to prevent environment stress and maintain normal physiological metabolism.

Efficacy and Safety of Anti-Tumor Necrosis Factor- Alpha Agents for Patients with Intestinal Behcet’s Disease: A Systematic Review and Meta-Analysis

Qingfeng Zhang,Chunyan Ma,Rongrong Dong,Weizhen Xiang,Meiqi Li,Zhenzhen Ma,Qingrui Yang 연세대학교의과대학 2022 Yonsei medical journal Vol.63 No.2

Purpose: Intestinal Behcet’s disease (BD) is a systemic autoimmune disease for which treatment options are limited. As a prospectivetherapeutic strategy for intestinal BD, anti-tumor necrosis factor-alpha (anti-TNF-α) agents have received increasing attention. In this study, we conducted a systematic review and meta-analysis to evaluate the efficacy and safety of anti-TNF-αagents for patients with intestinal BD. Materials and Methods: We searched PubMed, Embase, and Cochrane Library databases up to July 1, 2021 and articles that metthe eligibility criteria were further assessed. Pooled rates were synthesized by a randomized effects model using Stata software. Results: Eleven clinical trials covering 671 patients with intestinal BD were included. According to compositive data, the pooledrate for remission was 39% [95% confidence interval (CI) 26–52] in patients receiving anti-TNF-α agents. Intestinal symptomswere cured in 70% (95% CI 53–84) of the patients, and the rate for endoscopic healing was 65% (95% CI 52–78). Corticosteroid discontinuationwas achieved in 43% (95% CI 28–58) of the patients, and the dose reduction of corticosteroid was 20.43 mg (95% CI13.4–27.46). There were 239 adverse events and 80 serious adverse events during follow-up. Conclusion: Our study indicated that anti-TNF-α agents may serve as an effective treatment with acceptable safety for patientswith intestinal BD. However, more robust evidence from randomized controlled trials is urgently needed to assess the long-termefficacy and safety of anti-TNF-α agents for those patients.

Corn Peptides Protect Against Thioacetamide-Induced Hepatic Fibrosis in Rats

Jie Lv,Zhi-Kui Nie,Jiu-Liang Zhang,Feng-Yan Liu,Zhenzhen Wang,Zhi-Li Ma,Hui He 한국식품영양과학회 2013 Journal of medicinal food Vol.16 No.10

Certain bioactive peptides are reported to be able to alleviate hepatic fibrosis. Our previous work has confirmed the hepatoprotective effect of corn peptides (CPs) that are prepared from a high protein by-product, corn gluten meal, on acute liver injury in an animal model. However, the antifibrotic activity of CPs remained to be elucidated. In this study, the hepatoprotective effect of CPs on thioacetamide (TAA)-induced liver fibrosis was tested. Results showed that CPs (100 mg/kg body weight) significantly decreased the levels of alanine transaminase/aspartate transaminase, laminin, type IV collagen, and type III collagen in serum and increased the serum albumin levels and total antioxidant capacity. Additionally, with CP treatment (100 mg/kg body weight), a significant decrease was observed in the levels of malondialdehyde, nitric oxide, hydroxyproline, transforming growth factor b1, and lactate dehydrogenase activity as well as the liver index, while the activity of superoxidedismutase was significantly increased in livers. The histological and morphological analysis showed that the hepatocyte structure in CP-treated rats was superior to that of TAA-injured rats, and inflammation and fibrosis were also ameliorated. Therefore, CPs can be used as an option for prevention and adjuvant therapy of liver fibrosis.

Simultaneous regulation of photoabsorption and ferromagnetism of NaTaO3 by Fe doping

Huan Yang,Liguo Zhang,Lifang Yu,Fang Wang,Zhenzhen Ma,Jie Zhou,Xiaohong Xu 한국물리학회 2018 Current Applied Physics Vol.18 No.11

NaTa1-xFexO3 (0≤x≤0.40) nanocubes were synthesized by a relatively low temperature hydrothermal method, using Ta2O5, FeCl3 and NaOH as the precursors. The UV–vis diffuse reflectance spectra showed that NaTa1-xFexO3 had significant visible-light-absorbing capability, and the absorption edge of NaTaO3 shifted to longer wavelength with the increase of Fe dopants. Moreover, NaTa1-xFexO3 exhibited room-temperature ferromagnetism when Fe3+ occupied Ta5+ sites in NaTaO3 crystal lattice. The ferromagnetism is mainly attributed to the superexchange interactions between doped Fe3+, rather than the contribution of oxygen vacancies caused by Fe doping. Therefore, Fe doping can simultaneously regulate the optical and magnetic properties of NaTaO3 semiconductor, which will enable its potential applications in multifunctional optical-electronics and opticalspintronics devices.