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Yukyung Choi,Heeyoung Lee,Soomin Lee,Sejeong Kim,Jeeyeon Lee,Jimyeong Ha,Hyemin Oh,Yohan Yoon 한국축산식품학회 2017 한국축산식품학회지 Vol.37 No.6
In the present study, centrifugation and filtration pretreatments were evaluated to decrease sample preparation time and to improve the sensitivity and specificity of multiplex polymerase chain reaction (PCR) for the detection of low levels of pathogenic Escherichia coli in various foods. Pathogenic E. coli (E. coli NCCP11142, E. coli NCCP14037, E. coli NCCP 14038, E. coli NCCP14039, and E. coli NCCP15661) was inoculated into pork, beef, and baby leafy vegetables at 1, 2, and 3 Log CFU/g. The samples were shaken 30 times (control), then centrifuged or filtered. DNA extracts from the samples were subjected to PCR using the PowerchekTM Diarrheal E. coli 8-plex Detection Kit. In the pork samples, no E. coli was detected in the control samples, while E. coli were detected in 100% of 3-Log CFU/g inoculated and centrifuged samples, and in 100% of 2 and 3-Log CFU/g inoculated, and filtered samples. In the beef samples, all control samples appeared to be E. coli-negative, while E. coli was detected in 50-75% of centrifuged samples, regardless of inoculated level, and in 100% of 2 and 3-Log CFU/g inoculated, and filtered samples. In baby leafy vegetables, E. coli were not detected in 25-50% of the control samples, while E. coli were detected in 0-25% of the centrifuged samples, and 75-100% of the filtered samples, depending on the inoculum amount. In conclusion, filtration pretreatment can be used to minimize sample preparation time, and improve the sensitivity and specificity of rapid detection of pathogenic E. coli in various foods.
Choi, Yukyung,Lee, Yewon,Lee, Soomin,Kim, Sejeong,Lee, Jeeyeon,Ha, Jimyeong,Oh, Hyemin,Shin, Il-Shik,Yoon, Yohan The Korean Society of Fisheries and Aquatic Scienc 2019 Fisheries and Aquatic Sciences Vol.22 No.11
Background: The monitoring of pathogens of fishery auction markets is important to obtain safe fishery products regarding hygiene and sanitation. In this study, aerobic, coliform, Escherichia coli, and Vibrio cholerae were monitored in the fishery products and environmental samples obtained from fishery auction markets. Methods: The fishery products (flounder, octopus, skate, rock cod, sea bass, snail, monkfish, flatfish, comb pen shell, corb shell, conger eel, hairtail, croaker, and pilchard) were placed in filter bags, and the environmental samples (samples from the water tanks at the fishery auction markets, seawater from the fishery distribution vehicles, ice from wooden or plastic boxes, and surface samples from wooden and plastic boxes used for fish storage) were collected. Aerobic bacteria, E. coli, and coliform in the samples were enumerated on aerobic count plates and E. coli/coliform count plates, respectively. For V. cholerae O1 and V. cholerae non-O1 quantification, most probable number (MPN)-PCR analysis was performed. Results: Aerobic and coliform bacteria were detected in most samples, but E. coli was not detected. Wooden boxes were contaminated with high levels of aerobic and coliform bacteria in all seasons (spring, summer, and fall). During fall, V. cholerae non-O1 were detected in snails, hairtails, croakers, flatfishes, pilchards, plastic boxes, and water samples. Conclusions: These results indicate an increased prevalence of V. cholerae contamination in fishery products in fall, including food contact samples, which can be vehicles for cross-contamination.
Yukyung Choi,Sujung Lee,Heeyoung Lee,Soomin Lee,Sejeong Kim,Jeeyeon Lee,Jimyeong Ha,Hyemin Oh,Yewon Lee,Yujin Kim,Yohan Yoon 한국축산식품학회 2018 한국축산식품학회지 Vol.38 No.4
The objective of this study was to determine the minimum enrichment time for different types of food matrix (pork, beef, and fresh-cut lettuce) in an effort to improve Escherichia coli detection efficiency. Fresh pork (20 g), beef (20 g), and freshcut lettuce (20 g) were inoculated at 1, 2, and 3 Log CFU/g of Escherichia coli. Samples were enriched in filter bags for 3 or 5 h at 44.5℃, depending on sample type. E. coli cell counts in the samples were enriched in E. coli (EC) broth at 3 or 5 h. One milliliter of the enriched culture medium was used for DNA extraction, and PCR assays were performed using primers specific for uidA gene. To detect E. coli (uidA) in the samples, a 3–4 Log CFU/mL cell concentration was required. However, E. coli was detected at 1 Log CFU/g in fresh pork, beef, and fresh-cut lettuce after 5, 5, and 3-h enrichment, respectively. In conclusion, 5-h enrichment for fresh meats and 3-h enrichment for fresh-cut lettuce in EC broth at 44.5℃, and PCR analysis using uidA gene-specific primers were appropriate to detect E. coli rapidly in food samples.
산야초 추출물을 함유한 식사 대용식을 이용한 초저열량 식사요법이 성인 비만 여성들의 체중감량과 건강 개선에 미치는 영향
장유경(Yukyung Chang),김지영(Jiyoung Kim),김상연(Sangyeon Kim),최형석(Hyeongsuk Choi),최승(Sung Choi),박미현(Mihyeon Park),홍성길(Seonggil Hong),이무영(Moo-Young Lee),황성주(Sungjoo Hwang) 한국식품영양과학회 2005 한국식품영양과학회지 Vol.34 No.1
본 연구는 산야초 추출물이 함유된 식사대용식을 이용한 초저열량 식사요법의 체중감량과 건강개선에 미치는 효과를 평가하고자 실시되었다. 그 결과 식사대용식을 이용하여 초저열량 식사요법을 한 성인 비만여성들의 경우 일반 음식으로만 초저열량 식사요법을 한 성인 비만여성들보다 체중과 체지방율이 유의하게 감소하였음을 알 수 있었다. 건강관련 인자는 두 군간에 유의한 차이는 없었으나 산야초 추출물이 함유된 식사대용식을 섭취한 군내에서 식사중재 기간 동안 유의하게 지질 개선 효과가 있었다. 한편, 산야초 추출물이 함유된 식사대용식의 맛에 대한 기호도도 긍정적으로 평가되었다. 그러므로, 본 연구에서 사용된 산야초 추출물을 주성분으로 하여 제조된 식사대용식은 향후 임상에서 비만치료제로 응용함에 있어 크게 도움이 될 것으로 사료된다. 단, 앞으로 요요 현상의 가능성에 대한 보다 장기간에 걸친 임상연구가 이루어져야 할 것으로 사료된다. This study was performed to investigate the effects of very low calorie diet (VLCD) using meal replacements that contain the wild grass extracts that are consisted of mainly Samul-tang ingredients on weight reduction and health promotion in the obese women (BMI≥25㎏/㎡) for four weeks. Fifty six premenopausal women participated in this experiment. Each subject was either classified as part of the control group (C group, n=28) that consumed 3 regular meals a day or the meal replacement group (MR group, n=28) that consumed 1 regular meal and 2 meal replacements within 600 kcal/day. Anthropometric measurements, body composition, health related factors, and psychological factors were assessed before (the initial) and after (the 4^(th) week) the study. Obesity was significantly decreased after the diet intervention. Anthropometric measurements such as weight, waist and hip circumference, and BMI and body composition such as body fat, and fat mass were significantly decreased in all groups after the diet intervention. Anthropometric measurements and body composition of the MR group were significantly decreased more than those of the C group. Serum total cholesterol was significantly decreased in the MR groups but not in the C group and serum triglyceride concentration was significantly decreased in all groups after the diet intervention. All the biochemical measurements were within normal ranges after the diet intervention. Psychological factors such as stress and depression were significantly decreased in two groups after the diet intervention. Therefore, VLCD using meal replacements is very effective for weight reduction in the obese premenopausal women.
최유경(Yukyung Choi),나성권(SyungKwon Ra),김창환(ChangHwan Kim),박민용(Mignon Park),박성기(Sung-Kee Park) 대한전자공학회 2007 대한전자공학회 학술대회 Vol.2007 No.7
In this paper, we introduce a new remote meeting system which makes a robot imitate human arm motions captured from a remote space. For the real-time performance and accuracy, we propose a modified potential function of metaballs, downsized point cloud data. We have implemented a tangible interface using our method and successfully performed speed and accuracy test.
Mainstreaming of Health Equity in Infectious Disease Control Policy During the COVID-19 Pandemic Era
Choi, Hongjo,Kim, Seong-Yi,Kim, Jung-Woo,Park, Yukyung,Kim, Myoung-Hee The Korean Society for Preventive Medicine 2021 Journal of Preventive Medicine and Public Health Vol.54 No.1
The Korean government's strategy to combat coronavirus disease 2019 (COVID-19) has focused on non-pharmaceutical interventions, such as social distancing and wearing masks, along with testing, tracing, and treatment; overall, its performance has been relatively good compared to that of many other countries heavily affected by COVID-19. However, little attention has been paid to health equity in measures to control the COVID-19 pandemic. The study aimed to examine the unequal impacts of COVID-19 across socioeconomic groups and to suggest potential solutions to tackle these inequalities. The pathways linking social determinants and health could be entry points to tackle the unequal consequences of this public health emergency. It is crucial for infectious disease policy to consider social determinants of health including poor housing, precarious working conditions, disrupted healthcare services, and suspension of social services. Moreover, the high levels of uncertainty and complexity inherent in this public health emergency, as well as the health and socioeconomic inequalities caused by the pandemic, underscore the need for good governance other than top-down measures by the government. We emphasize that a people-centered perspective is a key approach during the pandemic era. Mutual trust between the state and civil society, strong accountability of the government, and civic participation are essential components of cooperative disaster governance.
Yukyung Jung,Jae Ho Han,Hyo Jung Choi,Jae Hee Lee 한국청각언어재활학회 2022 Audiology and Speech Research Vol.18 No.4
Purpose: The aim of this study was to examine test-retest reliability and validity of the adaptive Korean matrix sentence-in-noise test for elderly group with normal hearing (ENH) and elderly group with hearing impairment (EHI). Methods: Twenty ENH listeners and 20 EHI listeners based on the World Health Organization (WHO)-proposed hearing-impairment grade participated. For the test-retest reliability of the Korean matrix sentence-in-noise test, each subject was assessed twice in a 2-week interval using three types of noise: speech-shaped noise, International Collegium of Rehabilitative Audiology noise, and International Speech Test Signal. The test-retest agreement was judged by the intraclass correlation coefficient (ICC) and Bland-Altman plots. To differentiate between real change and random measurement error, the standard error of measurement (SEM) and the minimal detectable change (MDC) were also obtained as another reliability index. For the concurrent validity, the results of the Matrix test were compared to the results of the Korean-version Hearing In Noise Test (K-HINT). Results: The test-retest reliability was good to excellent for ENH and EHI groups (ICC, 0.84~0.98). Both groups showed stable reliability, but the ENH group showed smaller SEM and MDC values than the EHI group. No systematic errors were found from the Bland- Altman plots. The correlation between the results of the Matrix test and the K-HINT test was high, revealing good concurrent validity. Conclusion: The Korean matrix sentence-in-noise test appeared to be valid and reliable for the elderly. The Korean matrix sentence-in-noise test can be utilized for clinical measurement of the speech-in-noise ability of elderly individuals.