The Pathological Role of Astrocytic MAOB in Parkinsonism Revealed by Genetic Ablation and Over-expression of MAOB

An Heeyoung,허준영,C. Justin Lee,남민호 한국뇌신경과학회 2021 Experimental Neurobiology Vol.30 No.2

The cause of Parkinson’s disease has been traditionally believed to be the dopaminergic neuronal death in the substantia nigra pars compacta (SNpc). This traditional view has been recently challenged by the proposal that reactive astrocytes serve as key players in the pathology of Parkinson’s disease through excessive GABA release. This aberrant astrocytic GABA is synthesized by the enzymatic action of monoamine oxidase B (MAOB), whose pharmacological inhibition and gene-silencing are reported to significantly alleviate parkinsonian motor symptoms in animal models of Parkinson’s disease. However, whether genetic ablation and over-expression of MAOB can bidirectionally regulate parkinsonian motor symptoms has not been tested. Here we demonstrate that genetic ablation of MAOB blocks the MPTP-induced augmentation of astrocytic GABA-mediated tonic inhibition of neighboring dopaminergic neurons as well as parkinsonian motor symptoms, indicating the necessity of MAOB for parkinsonian motor symptoms. Furthermore, we demonstrate that GFAP-MAOB transgenic mice, in which MAOB is over-expressed under the GFAP promoter for astrocyte-specific over-expression, display exacerbated MPTP-induced tonic inhibition and parkinsonian motor symptoms compared to wild-type mice, indicating the importance of astrocytic MAOB for parkinsonian motor symptoms. Our study provides genetic pieces of evidence for the causal link between the pathological role of astrocytic MAOB-dependent tonic GABA synthesis and parkinsonian motor symptoms.

Analysis of Socio-Cultural Attitude towards Appearance and Related of MZ Generation Using Potential Profile Analysis

Heeyoung Lee(Heeyoung Lee),Eunsook Kim(Eunsook Kim) J-INSTITUTE 2022 Public Value Vol.7 No.2

Purpose: The purpose of this study is to examine and understand the types of consumers in their teens through their 30s who care for their appearance by analyzing the socio-cultural attitude types and related factors towards appearance using the potential profile analysis, and provide a practical assistance for marketing in line with the consumer type. Method: In this study, 520 online questionnaires were collected from among those in their teens and their 30s, and using SPSS 25ver and M-plus 7.0 programs, the basic analyses including frequency analysis, descriptive sta-tistical analysis, etc., as well as appearance using potential profile analysis were performed while cross-analysis and variance analysis were performed to identify differences in major variables according to the socio-cultural attitude type related classification and derived types. Results: First, as a result of the potential profile analysis, 4 groups were found to be the most appropriate, and the graphs were named 'overall low group', 'media awareness centric group', 'overall high group', and 'other perspective centric group'. As the main results of the difference test, the older the age group and the more office workers, the more likely they were to belong to the group that emphasized the point of view of others, while the interest in skin beauty and appearance were highly likely to belong to the general high-class and media aware-ness-centric group. That is, in the Korean society, there is a culture that emphasizes how you appear to others rather than yourself as you get older and enter social life, but this tendency does not have a positive effect on your interest in skin care or appearance. Whereas, such tendency can be interpreted as not having a positive effect on interest in skin beauty or appearance. Conclusion: According to the results of this study, the age group which entered society and occupation, etc. are highly likely to belong to the other-centric group because appearance is important, but the interest in ap-pearance and skin beauty tends to be higher in the media recognition-centric group. It can be seen that when it comes to taking care of one's appearance, it is more important for one to recognize the necessity through the media than to emphasize the interest of others. Hence, through this study, it is intended to be used as a promo-tional marketing strategy and provide services desired by consumers to increase their satisfaction and assistance lead to their reuse.

The Mediating Effect of Socio-Cultural Attitude towards Appearance on the Appearance Interest in the Relationship between Acne Perception and Appearance Satisfaction

Heeyoung Lee(Heeyoung Lee),Eunsook Kim(Eunsook Kim) J-INSTITUTE 2022 Protection Convergence Vol.7 No.2

Purpose: The purpose of this study was to examine and understand the mediating effect of socio-cultural attitude towards appearance on the appearance interest in the relationship between acne perception and appearance satisfaction, and provide a practical assistance for the programs for the acne skin in the beauty industry and market. Method: In this study, the statistical analysis was conducted using the SPSS 25.0 and AMOS 22.0 programs of 282 online questionnaires for the subjects with acne skin. Results: As for the results of this study, the socio-cultural attitude towards appearance has had a positively significant impact on the appearance interest(β=.453, p<.001), acne perception(β=.149, p<.05), and appearance satisfaction(β=.535, p<. 001), and the direct effect of socio-cultural attitude towards appearance on the appearance satisfaction was negatively significant(β=-.362, p<.001), while having a positively significant effect on the acne perception(β=.149, p<.05). Furthermore, in the relationship between the socio-cultural attitude towards appearance and appearance satisfaction, the interest in appearance demonstrated a positive mediating effect. This is a result demonstrating that if a person's socio-cultural attitude towards appearance is large, he or she can directly cause dissatisfaction with one's appearance, yet if one's interest in the appearance is raised before then, the appearance satisfaction might also be increased as a result. Conclusion: Based on the results of this study, it was confirmed that the larger the socio-cultural attitude towards the appearance, the larger the appearance interest and acne perception scores, and the larger the appearance interest, the larger the appearance satisfaction. Meanwhile, the socio-cultural attitude towards appearance negatively and significantly impacts the appearance satisfaction. Hence, it is apparent that the overall appearance is important as it impacts the appearance interest, not the skin type, and when consulting with the customers with acne skin, it is necessary to present the direction of interest in the appearance and suggest that it is also important to accurately perceive one's own skin condition. Through which, it is intended that the direction of social and cultural attitude towards appearance and interest in the appearance are presented to provide the services desired by consumers to increase their satisfaction with their appearance, and furthermore, provide assistance for the practical on-site management programs and counseling skills for acne skin in the medical skin care beauty industry and market.

Quantitative Microbial Risk Assessment for Campylobacter jejuni in Ground Meat Products in Korea

Lee, Jeeyeon,Lee, Heeyoung,Lee, Soomin,Kim, Sejeong,Ha, Jimyeong,Choi, Yukyung,Oh, Hyemin,Kim, Yujin,Lee, Yewon,Yoon, Ki-Sun,Seo, Kunho,Yoon, Yohan Korean Society for Food Science of Animal Resource 2019 한국축산식품학회지 Vol.39 No.4

This study evaluated Campylobacter jejuni risk in ground meat products. The C. jejuni prevalence in ground meat products was investigated. To develop the predictive model, survival data of C. jejuni were collected at $4^{\circ}C-30^{\circ}C$ during storage, and the data were fitted using the Weibull model. In addition, the storage temperature and time of ground meat products were investigated during distribution. The consumption amount and frequency of ground meat products were investigated by interviewing 1,500 adults. The prevalence, temperature, time, and consumption data were analyzed by @RISK to generate probabilistic distributions. In 224 samples of ground meat products, there were no C. jejuni-contaminated samples. A scenario with a series of probabilistic distributions, a predictive model and a dose-response model was prepared to calculate the probability of illness, and it showed that the probability of foodborne illness caused by C. jejuni per person per day from ground meat products was $5.68{\times}10^{-10}$, which can be considered low risk.

Comparative analysis between combination and single-agent chemotherapy for elderly patients with advanced non-small cell lung cancer: A nationwide population-based outcome study

Lee, Yun-Gyoo,Lee, Ju Hyun,Kim, Se-Hyun,Kim, Yu Jung,Lee, Heeyoung,Ahn, Soyeon,Jang, Joung-Soon,Lee, Jong-Seok,Kim, Jee Hyun Elsevier 2018 Lung cancer Vol.122 No.-

<P><B>Abstract</B></P> <P><B>Introduction</B></P> <P>To determine whether an upfront combination compared to single-agent therapy is beneficial for elderly patients with advanced non-small cell lung cancer (NSCLC) in the real world, a population-based epidemiologic study was conducted.</P> <P><B>Methods</B></P> <P>Patients ≥70 years with advanced NSCLC from 2007 to 2012 were identified in the National Health Insurance Service Database of Korea. A Cox proportional-hazards regression model and propensity score analysis were used to examine the effect of treatment modality on survival.</P> <P><B>Results</B></P> <P>Among 41,276 patients newly diagnosed with lung cancer, 8274 (20.0%) identified to be treated with upfront palliative chemotherapy were eligible for this study. After excluding 976 patients who received a first-line anti-epidermal growth factor receptor (EGFR) treatment, 7298 (88.2%) who received cytotoxic chemotherapy were included in further analyses: 5636 (77.2%) received doublet chemotherapy and 1662 (22.8%) received monotherapy. The most frequent regimen in combination group was gemcitabine and platinum doublet (44.7%), whereas that in monotherapy group was gemcitabine (46.7%). Multivariate analyses indicated lower use of combination chemotherapy with increasing age (odds ratio [OR] 0.73; 95% CI 0.67–0.79; <I>P</I> < 0.001) and female sex (OR 0.71; 95% CI 0.62–0.80; <I>P</I> < 0.001). Receipt of combination over single-agent chemotherapy was associated with a reduced risk of death (hazard ratio [HR] 0.91; 95% CI 0.86–0.96; <I>P</I> = 0.001) in overall population and (HR 0.89; 95% CI 0.80–0.98; <I>P</I> = 0.019) in the propensity-matched cohort.</P> <P><B>Conclusion</B></P> <P>In elderly patients with advanced NSCLC excluding those receiving frontline anti-EGFR targeted agents, receiving initial combination chemotherapy compared to single-agent was associated with improved survival.</P> <P><B>Highlights</B></P> <P> <UL> <LI> This population-based study included all patients ≥70 years of advanced NSCLC. </LI> <LI> We examined if an upfront combination compared to singlet therapy is beneficial. </LI> <LI> Combination over singlet chemotherapy was associated with a reduced risk of death. </LI> <LI> Our results were consistent between overall and propensity score-matched cohort. </LI> </UL> </P>

Combination of Hydrophobic Filtration and Enrichment Methods for Detecting Bacillus cereus in Fresh-Cut Cabbage

Lee, Sujung,Choi, Yukyung,Lee, Heeyoung,Kim, Sejeong,Lee, Jeeyeon,Ha, Jimyeong,Oh, Hyemin,Lee, Yewon,Kim, Yujin,Yoon, Yohan,Lee, Soomin The Korean Society of Food Hygiene and Safety 2018 한국식품위생안전성학회지 Vol.33 No.5

본 연구에서는 신선편이 양배추 내 Bacillus cereus의 최적 증균 온도를 선정하고 증균배양액에 소수성필터를 적용하여 multiplex PCR의 검출률을 확인하였다. B. cereus 증균온도는 B. cereus 균주 5 개를 혼합하여 1 Log CFU/mL이 되도록 증균배지에 접종하고 $30^{\circ}C$, $37^{\circ}C$, $42^{\circ}C$에서 증균한 뒤 3시간 간격으로 MYP agar에 도말한 후 계수하여 선정하였다. 소수성필터 미적용 그룹은 B. cereus 균주 5 개를 혼합하여 신선편이 양배추에 접종한 뒤 최적 증균온도에서 증균하였으며, 증균배양액을 가열하여 DNA를 추출한 뒤 multiplex PCR을 진행하였다. 소수성필터 적용 그룹은 증균배양액을 소수성 필터에 적용하고 필터에 있는 균을 멸균증류수로 현탁한 뒤 가열하여 추출된 DNA로 multiplex PCR을 진행하였다. 증균온도 확인 결과, 6시간 증균 시 $42^{\circ}C$에서 증균된 샘플($5.4{\pm}0.3Log\;CFU/mL$)과 $30^{\circ}C$에서 증균된 샘플($4.6{\pm}0.6Log\;CFU/mL$) 간 유의차가 확인되었다(p < 0.05). 소수성필터 적용 유무에 따른 multiplex PCR 결과, 1 Log CFU/g 접종된 샘플의 검출률이 소수성 필터 적용 전 60%(3/5)에서 100%(5/5)로 향상되었다. 2 Log CFU/g 접종 샘플은 소수성필터 적용 전 80%(4/5)에서 소수성 필터 적용 후 100%(5/5)로 검출률이 증가하였으나, 3 Log CFU/g 접종 샘플은 소수성 필터 적용 전후 모두 100%(5/5)로 확인되었다. 이상의 결과를 통해 신선편이 양배추 내 B. cereus 검출 시 증균배양액에 소수성필터를 적용하고 multiplex PCR을 적용했을 때 신속하고 효율적인 검출이 가능할 것으로 판단된다. This study developed a rapid detection method for Bacillus cereus in fresh-cut cabbages. Fresh-cut cabbage samples were inoculated at 1-, 2- and 3-Log CFU/g, and pathogens were enriched in tryptic soy broth containing 0.15% polymyxin B at $30^{\circ}C$, $37^{\circ}C$, and $42^{\circ}C$ to determine the detection limit and appropriate enrichment temperature for multiplex PCR detection. Enriched bacterial cells in enrichment broth were collected in a hydrophobic filter prior to DNA extraction for multiplex PCR. Filters were resuspended in distilled water, and DNA was extracted from the suspension. DNA samples were further analyzed by multiplex PCR. Detection limit of multiplex PCR was 5-Log CFU/mL. B. cereus cell counts were higher (P < 0.05) at $42^{\circ}C$ than other temperatures. Detection rate of 1-, 2-, and 3-Log CFU/g inoculated samples were 60%, 80%, and 100% after enrichment respectively. However, when enriched samples were filtered with hydrophobic membrane filter, detection rates became 100%, regardless of inoculation level. Results indicate a combination of enrichment with hydrophobic filtration improves rapid detection efficiency of B. cereus in fresh-cut cabbage by multiplex PCR.

Quantitative Microbial Risk Assessment for Clostridium perfringens in Natural and Processed Cheeses

Lee, Heeyoung,Lee, Soomin,Kim, Sejeong,Lee, Jeeyeon,Ha, Jimyeong,Yoon, Yohan Asian Australasian Association of Animal Productio 2016 Animal Bioscience Vol.29 No.8

This study evaluated the risk of Clostridium perfringens (C. perfringens) foodborne illness from natural and processed cheeses. Microbial risk assessment in this study was conducted according to four steps: hazard identification, hazard characterization, exposure assessment, and risk characterization. The hazard identification of C. perfringens on cheese was identified through literature, and dose response models were utilized for hazard characterization of the pathogen. For exposure assessment, the prevalence of C. perfringens, storage temperatures, storage time, and annual amounts of cheese consumption were surveyed. Eventually, a simulation model was developed using the collected data and the simulation result was used to estimate the probability of C. perfringens foodborne illness by cheese consumption with @RISK. C. perfringens was determined to be low risk on cheese based on hazard identification, and the exponential model ($r=1.82{\times}10^{-11}$) was deemed appropriate for hazard characterization. Annual amounts of natural and processed cheese consumption were $12.40{\pm}19.43g$ and $19.46{\pm}14.39g$, respectively. Since the contamination levels of C. perfringens on natural (0.30 Log CFU/g) and processed cheeses (0.45 Log CFU/g) were below the detection limit, the initial contamination levels of natural and processed cheeses were estimated by beta distribution (${\alpha}1=1$, ${\alpha}2=91$; ${\alpha}1=1$, ${\alpha}2=309$)${\times}$uniform distribution (a = 0, b = 2; a = 0, b = 2.8) to be -2.35 and -2.73 Log CFU/g, respectively. Moreover, no growth of C. perfringens was observed for exposure assessment to simulated conditions of distribution and storage. These data were used for risk characterization by a simulation model, and the mean values of the probability of C. perfringens foodborne illness by cheese consumption per person per day for natural and processed cheeses were $9.57{\times}10^{-14}$ and $3.58{\times}10^{-14}$, respectively. These results indicate that probability of C. perfringens foodborne illness by consumption cheese is low, and it can be used to establish microbial criteria for C. perfringens on natural and processed cheeses.

Development of User-Friendly Modeling Software and Its Application in Processed Meat Products

Lee, Heeyoung,Lee, Panho,Lee, Soomin,Kim, Sejeong,Lee, Jeeyeon,Ha, Jimyeong,Choi, Yukyung,Oh, Hyemin,Yoon, Yohan The Korean Society of Food Hygiene and Safety 2018 한국식품위생안전성학회지 Vol.33 No.3

본 연구에서는 육제품의 다양한 조건(포장, 저장온도, 염농도, 아질산염농도)에서의 식중독세균의 생장을 예측하는소프트웨어를 예측미생물학에 대한 지식이 부족한 비전문가도 손쉽게 이용할 수 있도록 개발하였다. 육제품에서의 식중독세균예측소프트웨어(FAME: Foodborne bacteria Animal product Modeling Equipment)는 Javascript와 HTML을 이용하여 개발하였으며, 육제품에 대한 카이네틱모델과 확률모델을 포함하고있다. FAME에서는 검증(validation) 기능을 포함하고 있으며, FAME에 탑재 되어있는 예측모델의 수식을 자유롭게 수정할 수 있도록 고안 하였다. FAME에는 포장조건, 온도, 염농도, 아질산염농도 조합에 따라 실험한 데이터를 카이네틱모델(5,400 데이터)과 확률모델(345,600 데이터)에 탑재하였다. 사용자가 FAME을 이용하여 육제품의 제조 조건을 소프트웨어에 입력하면, 시간에 따른 식중독세균의 생장패턴과 생장확률이 즉시 계산 되어진다. 따라서 예측 미생물학에 대한 전문 지식이 없는 비전문가라고 하더라도 FAME을 이용하여 직접 실험을 하지 않고도 육제품에서의 식중독세균의 생장을 쉽고 빠르게 예측할 수 있어, 육가공분야에서 매우 유용하게 사용되어 질 수 있을 것으로 판단된다. The objective of this study was to develop software to predict the kinetic behavior and the probability of foodborne bacterial growth on processed meat products. It is designed for rapid application by non-specialists in predictive microbiology. The software, named Foodborne bacteria Animal product Modeling Equipment (FAME), was developed using Javascript and HTML. FAME consists of a kinetic model and a probabilistic model, and it can be used to predict bacterial growth pattern and probability. In addition, validation and editing of model equation are available in FAME. The data used by the software were constructed with 5,400 frankfurter samples for the kinetic model and 345,600 samples for the probabilistic model using a variety of combinations including atmospheric conditions, temperature, NaCl concentrations and $NaNO_2$ concentrations. Using FAME, users can select the concentrations of NaCl and $NaNO_2$ meat products as well as storage conditions (atmosphere and temperature). The software displays bacterial growth patterns and growth probabilities, which facilitate the determination of optimal safety conditions for meat products. FAME is useful in predicting bacterial kinetic behavior and growth probability, especially for quick application, and is designed for use by non-specialists in predictive microbiology.

Development of User-Friendly Modeling Software and Its Application in Processed Meat Products

Heeyoung Lee,Panho Lee,Soomin Lee,Sejeong Kim,Jeeyeon Lee,Jimyeong Ha,Hyemin Oh,Yohan Yoon,Yukyung Choi 한국식품위생안전성학회 2018 한국식품위생안전성학회지 Vol.33 No.3

The objective of this study was to develop software to predict the kinetic behavior and the probability of foodborne bacterial growth on processed meat products. It is designed for rapid application by non-specialists in predictive microbiology. The software, named Foodborne bacteria Animal product Modeling Equipment (FAME), was developed using Javascript and HTML. FAME consists of a kinetic model and a probabilistic model, and it can be used to predict bacterial growth pattern and probability. In addition, validation and editing of model equation are available in FAME. The data used by the software were constructed with 5,400 frankfurter samples for the kinetic model and 345,600 samples for the probabilistic model using a variety of combinations including atmospheric conditions, temperature, NaCl concentrations and NaNO2 concentrations. Using FAME, users can select the concentrations of NaCl and NaNO2 meat products as well as storage conditions (atmosphere and temperature). The software displays bacterial growth patterns and growth probabilities, which facilitate the determination of optimal safety conditions for meat products. FAME is useful in predicting bacterial kinetic behavior and growth probability, especially for quick application, and is designed for use by non-specialists in predictive microbiology.

Combination of Hydrophobic Filtration and Enrichment Methods for Detecting Bacillus cereus in Fresh-Cut Cabbage

Sujung Lee,Yukyung Choi,Heeyoung Lee,Sejeong Kim,Jeeyeon Lee,Jimyeong Ha,Hyemin Oh,Yewon Lee,Yujin Kim,Yohan Yoon,Soomin Lee 한국식품위생안전성학회 2018 한국식품위생안전성학회지 Vol.33 No.5

본 연구에서는 신선편이 양배추 내 Bacillus cereus의 최 적 증균 온도를 선정하고 증균배양액에 소수성필터를 적용하여 multiplex PCR의 검출률을 확인하였다. B. cereus 증균온도는 B. cereus 균주 5 개를 혼합하여 1 Log CFU/ mL이 되도록 증균배지에 접종하고 30oC, 37oC, 42oC에서 증균한 뒤 3시간 간격으로 MYP agar에 도말한 후 계수하여 선정하였다. 소수성필터 미적용 그룹은 B. cereus 균 주 5 개를 혼합하여 신선편이 양배추에 접종한 뒤 최적 증균온도에서 증균하였으며, 증균배양액을 가열하여 DNA 를 추출한 뒤 multiplex PCR을 진행하였다. 소수성필터 적용 그룹은 증균배양액을 소수성 필터에 적용하고 필터에 있는 균을 멸균증류수로 현탁한 뒤 가열하여 추출된 DNA 로 multiplex PCR을 진행하였다. 증균온도 확인 결과, 6시간 증균 시 42oC에서 증균된 샘플(5.4 ± 0.3 Log CFU/mL) 과 30oC에서 증균된 샘플(4.6 ± 0.6 Log CFU/mL) 간 유의 차가 확인되었다(p < 0.05). 소수성필터 적용 유무에 따른 multiplex PCR 결과, 1 Log CFU/g 접종된 샘플의 검출률이 소수성 필터 적용 전 60%(3/5)에서 100%(5/5)로 향상 되었다. 2 Log CFU/g 접종 샘플은 소수성필터 적용 전 80%(4/5)에서 소수성 필터 적용 후 100%(5/5)로 검출률이 증가하였으나, 3 Log CFU/g 접종 샘플은 소수성 필터 적용 전후 모두 100%(5/5)로 확인되었다. 이상의 결과를 통해 신선편이 양배추 내 B. cereus 검출 시 증균배양액에 소수성필터를 적용하고 multiplex PCR을 적용했을 때 신속하고 효율적인 검출이 가능할 것으로 판단된다. This study developed a rapid detection method for Bacillus cereus in fresh-cut cabbages. Fresh-cut cabbage samples were inoculated at 1-, 2- and 3-Log CFU/g, and pathogens were enriched in tryptic soy broth containing 0.15% polymyxin B at 30oC, 37oC, and 42oC to determine the detection limit and appropriate enrichment temperature for multiplex PCR detection. Enriched bacterial cells in enrichment broth were collected in a hydrophobic filter prior to DNA extraction for multiplex PCR. Filters were resuspended in distilled water, and DNA was extracted from the suspension. DNA samples were further analyzed by multiplex PCR. Detection limit of multiplex PCR was 5-Log CFU/mL. B. cereus cell counts were higher (P < 0.05) at 42oC than other temperatures. Detection rate of 1-, 2-, and 3-Log CFU/g inoculated samples were 60%, 80%, and 100% after enrichment respectively. However, when enriched samples were filtered with hydrophobic membrane filter, detection rates became 100%, regardless of inoculation level. Results indicate a combination of enrichment with hydrophobic filtration improves rapid detection efficiency of B. cereus in fresh-cut cabbage by multiplex PCR.